Virus 중화시험법에 의한 닭 빈혈성인자의 항체조사

류광선,고홍범,Ryoo, Gwang-seon,Koh, Hong-bum 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.2

A serological survey for antibody to chicken anemia agent(CAA) was carried out by virus neutralization test. Antibody to CAA was detected in broilers and layers at different age groups. The results obtained were summarized as follows ; 1. Of a total of 1,035 chicken sera from 1.16 flocks 617 samples of sera were detected as positive(59.6%) and 95 flocks of a total flocks as positive(81.9%). 2. Proportion of positive sera by age were 92.3 %(88.9~100%) at 1 to 2 weeks of age, 56.4%(16.7~77.8%) at 3 to 9 weeks of age, 85.0%(50.0~100%) at 10 to 14 weeks of age and all tested sera were positive at over the 15 weeks age. 3. In each broiler and layer chicken 63.6% and 68.4% chicks possessed positive sera respectively. 4. Neutralizing antibody titer in age group was various from 1:10 to 1:6,400 and mean titer was 1:400 to 1:800.

도축돈의 폐병변에서 Streptococcus suis 1 (+14)형, 2 (+1/2)형, 7형 그리고 9형의 Multiplex PCR을 통한 검출

구경민,임재향,고홍범,Koo, Kyung-Min,Lim, Jae-Hyang,Koh, Hong-Bum 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.4

Streptococcus suis is an important swine pathogen in nearly all countries with an extensive pig industry. It is associated with meningitis, arthritis, endocarditis, septicaemia, bronchopneumonia and sudden death. Attempts to control the disease are still hampered the lack of effective vaccines and sensitive diagnostic tools. A PCR method which can be used for the detection of virulent strains of serotype 2, which is most prevalent serotype, and serotype 1 was developed. However, serotype 1, 2, 7 and 9 strains are frequently isolated from diseased pigs. In European countries, S suis serotype 2 is the most prevalent type isolated from diseased pigs, followed by serotype 9 and 1. In Japan, capsular serotype 2 was also the most prevalent serotype, followed by capsular serotype 7. Most of S suis isolated from diseased pigs belong to a limited number of capsular serotype, often those between 1 and 9. We investigated the distribution of S suis serotype 1, 2, 7 and 9 from 740 pig lungs at abattoir in Jeolla and Chungcheong by rapid multiplex PCR assay. Fifty of 740 lung samples, 6.8%, were S suis postitive and identified S suis were divided by 38% (19/50) in serotype 2, 2% (1/50) in serotype 7 and 4% (2/50) in serotype 9. The distribution of S suis serotype in Korea was similar to other countries. Moreover, the multiplex PCR assay may be an useful diagnostic tool for the detection of pigs carrying serotype 1, 2, 7, 1/2, 9 and 14 strains in epidemiological and transmission studies and facilitate control and eradication programs.

국내 야생 집쥐(Rattus norvegicus)의 murine typhus 감염조사

김희선,강문일,고홍범,Kim, Hee-sun,Graham, Robert Ross,Kang, Mun-il,Koh, Hong-bum 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.4

Sixty eight wild rats, Rattus norvegicus, were caught from Seoul city, Kyonggi, Kangwon, Cholla, and Kyongsang-provinces. All sera collected from rats were examined by immunofluorescent test to find antibody against murine typhus. The antibody prevalance to murine typhus was 4.4%(3/68). Sero-positive rate was 12.5%(2/16) in Kyonggi province and 12.5%(1/8) in Cholla province. The sero-positive rate difference between sexes of rats was not recognized. Three Rattus norvegicus having antibody to murine typhus were morphologically classified as subadult with 8.3%(1/12), middle-aged adult with 5.9%(1/17), and old adult with 4.8%(1/21), respectively.

면역억압계군에서 Salmonella gallinarum 감염에 대한 면역반응

김계엽,임재향,고홍범,조경오,김태열,오명화,Kim, Gye-Yeup,Lim, Jae-Hyang,Koh, Hong-Bum,Cho, Kyoung-Oh,Kim, Tae-Youl,Oh, Myoung-Hwa 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.3

The immune response against Salmonella gallinarum infection was investigated in immunosuppresed chickens. Newly hatched chickens were treated with cyclophosphamide at the first and second day after birth and were challenged intraperitoneally with S gallinarum ($1{\times}10^7CFU/m{\ell}$) on day 6. Group 1, Immunosuppresed and Challenged group, was treated with cyclophaiphamide and challenged with S gallinarum; group 2, Immunosuppressed group, was only treated with cyclophsphamide; group 3, Challenged group, was only challenged with S gallinarum; group 4, Control group. In each group, the localization of lymphocytes of the lymphoid organs and intestine was immunohistochemically compared using a variety of monoclonal antiboies ($CD4^+$, $CD8^+$, and B lymphocyte). Also, S gallinarum were assessed by Maccallum-Goodpasture stain and immunohistochemical analysis in the paraffin-embedded intestinal tissues. In S gallinarum challenged chickens, $CD4^+$ and $CD8^+$ lymphocytes of the intestinal organs such as duodenum, jejunum, ileum and colon were increased. However, in cyclophophamide treated chickens, $CD4^+$ and $CD8^+$ lymphocytes and especially B lymphocytes of the lymphoid organs such as thymus, spleen, and bursa of Fabricius were dramatically decreased. These results suggest that cyclophsophamide is an immunosuppressive agent that especially causes depletion of B lymphocytes, suppress humoral immunity and eventually suppresses avian immune responses. Its protection against S gallinarum infection is mainly dependent on both cell-mediated mechanism and the humoral immune response.

도축돈에서 분리된 Streptococcus suis에 대한 연구

윤선종 ( Sun Jong Yoon ),고홍범 ( Hong Bum Koh ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.3

Streptococcus suis has been identified as a major cause of contagious disease in pigs Ithas been isolated worldwide from pigs suffering from meningitis, bronchopneumonia, polyserositis, polyarthritis and septicemia. Two hundred and fifty-five lung samples of slaughter pigs with gross lung lesions were collected from Jan. to Dec. 1995 in southern Chonnam area. Isolation of S suis were tested by biochemical, serological methods and antimicrobial susceptibility. The results were summerrized as follows; 1. S suis was isolated from 30 of 254 pneumonic lungs, 14 isolates were isolated only, 12 were isolated with P. multocida and 4 were isolated with unidentified Gram positive cocci. 2. In biochemical characteristics studies, all isolates were not grown in 6.5% NaCl medium, and most isolates utilized L-leucine-2-naphtylamide and trehalose, didn`t utilize sorbitol, ribose and L-arabinose. 3. In slide agglutination test, 6(20%) isolates were classified as serotype 2,4(13.3%) isolates were as serotype 1/2, 16,2 and 2(6.6%) were as serotype 1, 4, 13. 4. S suis isolates showed marked susceptibility to amoxicillin, oxacillin, cephalothin and cepofera-zone and high resistance to kanamycin, streptomycin, tetracycline and erythromycin.

국내 야생 집쥐(Rattus norvigicus)의 유행성 출혈열바이러스 감염증의 역학조사

김희선,이준학,강문일,고홍범,Kim, Hee-sun,Graham, Robert Ross,Lee, Jun-hak,Kang, Mun-il,Koh, Hong-bum 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.2

Sixty eight wild rats were caught from Seoul, Kyonggi, Kangwon, Cholla, and Kyongsang provinces in Korea. All of them were confirmed in the same species, Rattus norvigicus. All of sera from wild rats were examined by indirect immunofluorescent test against Hantaan-related virus. Detection of anti-body to Hantaan-related virus were 4 out of 68 rats(5.9%), including 2 of 8(25.0%) rats in Kangwon and 2 of 20 rats(10.0%) in Kyongsang province. No difference of the sero-positive rates between sex of rats tested to Hantaan-related virus was recognized. According to age, four rats with antibodies(1:320~640) to Hantaan-related virus belonged one subadult, one young adult, one middle aged adult, and one old adult, respectively.

국내 pasteurella anatipestifer 분리주의 오리에 대한 실험병리힉적 관찰

강문일(Mun Il Kang),고홍범(Hong Bum Koh) 한국예방수의학회 1993 예방수의학회지 Vol.17 No.1

The purpose of the study was pathologically to understand the pathogenecity of ducklings to a Korea isolate of Pasteurella anatipestifer (Pa) according to previous vaccination or not. One hundred eight ducklings were equally divided by six groups including 5 groups challenged with Pa after the inoculation of each killed vaccine prepared by undiluted (6×10⁹CFU/ml), ×5-, ×10-, ×20-, ×40- diluted concentrations of Pa, respectively, and one control group only inoculated with Pa without previous vaccination. Each bacterin was subcutaneously inoculated 2 times at 2 and 3-week old, and at 1 week after the final vaccination, all ducklings were subcutaneously inoculated with 0.5ml of Pa (6×10⁹CFU/ml) per head and sacrificed at 2 weeks later. Clinical signs were examined during the duration between the last vaccination and just before necropsy and all including dead and live cases during the experiment were carried out gross and histopathological examination. l. Among groups, the prevalence of clinical signs and mortality was gradually increased toward the groups inoculated with bacterins which made higher Pa-diluted concentration before challenge with Pa. Group treated with bacterin made by Pa-undiluted concentraion was no clinical signs and mortality, On the contrary, other groups with vaccination showed from 27.8% to 77.8% in clinical signs and from 11.1% to 27.8% in mortality. Control group was 100% clinical signs and 72.2% mortality. 2. Main clinical signs were early ocular discharge, greenish diarrhoea, sharp decrease of weight gain, ataxia, recumbency, torticollis, tremor of neck and death. 3. Principal gross findings were consisted of epicarditis and myocarditis, endocardial hemorrhage, increased volume of epicardial fluid with yellowish to red in colour, fibrinous airsacculitis and perihepatitis, mild splenohepatomegaly, pulmonary edema and congestion, petechiation of tracheal mucosa, and meningeal congestion and hemorrhage. 4. Main histopathological lesions comprised epicardium covered with fibrinous exudate including lots of heterophils, macrophages and homogenous eosinophilic fibrinous material, myocardium with edema, mononuclear cell infiltration and sometimes calcification in heart, diffuse vacuolar degeneration of hepatocytes and mononuclear cell infiltration in varing degrees of periportal areas in liver, mild hyperplasia of lymphocyte and reticular cells of spleen in live cases at autopy, but mild to marked deposition of eosinophilic substance and lymphocyte depletion in white pulp and compression of red pulp of spleen in dead case, mild to marked meningitis with many lymphocytes and few heterophils, perivascular lymphocytic infiltraion in brain and spinal cord, diffuse edema and congestion of interlobular space and lymphocytic hyperplasia around parabronchus of lungs. 5. Generally, the group treated with bacterin prepared by Pa-undiluted concentration prior to challenge showed milder severity and degree of pathological lesions than those in the other groups with vaccination. Grossly, the former was recorded just as 5.6% in their prevalence, but the latter was ranged from 50.0% to 88.9%.

Staphylococcus aureus에 의한 유방염에 대한 β-lactamase 저해제/β-lactam계 항균제 치료 효과

임숙경,임재향,주이석,문진산,이애리,고홍범,Lim, Suk-Kyung,Lim, Jae-Hhyang,Joo, Yi-Seok,Moon, Jin-San,Lee, Ae-Ri,Koh, Hong-Bum 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.1

The antimicrobial effect of ${\beta}$-lactam antibiotics, which had ${\beta}$-lactamase inhibitor activity, on Staphylococcus aureus isolated from mastitis was investigated in this study. Out of 166 isolates, 99 isolates (59.6%) produced ${\beta}$-lactamase, and 98 isolates of 99 were ${\beta}$-lactamase positive in above $12.5{\mu}g/m{\ell}$ MIC of penicillin. In the providence distribution, ${\beta}$-lactamase production rate of 4 providence, Gangwon, Gyeonggi, Chungcheong, and Jeolla was 100%, 65.7%, 58.8%, and 50.0%, respectively. Antibiotic activities of ${\beta}$-lactam antibiotics against lactamase positive isolates also were investigated. Antimicrobial effects of ampicillin/sulbactam or amoxicillin/clavulanic acid treated group were better than ampicillin or amoxicillin treated group. In antimicrobial effects on intracellular S aureus, there was no difference 1 hour and 4 hour treatment in control, ampicillin, and amoxicillin group, but in 18 hours treatment, ampicillin/sulbactam or amoxicillin/clavulanic acid had a better effect than ampicillin or amoxicillin (p<0.05).

중합효소연쇄반응을 이용한 돼지 증식성 장염 진단기법 확립

임숙경,이희수,우승룡,윤순식,문운경,이유영,고홍범,Lym, Suk-kyung,Lee, Hee-soo,Woo, Sung-ryong,Yoon, Soon-seek,Moon, Oun-kyong,Lee, Yoo-young,Koh, Hong-bum 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.1

Porcine Proliferative Enteropathy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 to 20 weeks of age. PPE has been diagnosed by clinical signs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs. Therefore, in this study, we established the polymerase chain reaction(PCR) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonuclease analysis with restriction enzyme Hae III and Pst I. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.

키토산올리고당 기질-유기태 아연(Zn-OCHT) 섭이에 의한 산란계 계란 고품질 생산성 연구

조향현 ( Hyang Hyun Cho ),김희경 ( Hee Kyong Kim ),고홍범 ( Hong Bum Koh ),김영호 ( Young Ho Kim ) 한국키틴키토산학회 2016 한국키틴키토산학회지 Vol.21 No.1

In this study, we investigated whether it is possible to substitute the inorganic zinc of chitosan oligosaccharide substrate organic zinc. We investigated the quality characteristics of egg produced from laying hens with non-supplemented diets or diets supplemented with organic zinc on chitosan oligosaccharide. Organic-zinc was obtained from substrate of chitosan oligosaccharide. Organic zinc or non-organic zinc were mixed normal laying hen diet. Laying hens were fed diet for 45 days containing powdered chitosan oligosaccharide-Zn, inorganic zinc or normal feedstuff. During the experimental period, organic zinc group increased the egg shell thickness, egg heights and lymphocyte compared to inorganic zinc or aminated form products (p<0.05). And, edible weight and yolk colors were no significant difference. But, each effectiveness are different from organic-zinc concentration to feeding period. It was investigated that organic zinc had equal or more effects compared with inorganic zinc or normal diet. These results suggest that organic zinc could be used as substitute for inorganic zinc as well as the supplement of laying hens diets to produce functional eggs.