화장품(에멀젼형)에서 Pseudomonas aeruginosa의 성장과 방부살균제효과

류미숙(Mi Sook Ryu),김장규(Jang Kyu Kim),김남기(Nam Ki Kim) 한국생물공학회 1992 KSBB Journal Vol.7 No.2

정신건강전문요원의 수련 및 근무 현황

이상훈(Sanghoon Lee),송열매(Yul-Mai Song),우지연(Ji-Yeon Woo),한정미(Jeongmi Han),류미숙(Mi-Sook Ryu),황태연(Tae-Yeon Hwang) 대한사회정신의학회 2017 사회정신의학 Vol.22 No.2

연구목적 : 개정된 정신건강복지법이 도입되면서, 지역사회에서의 정신건강전문요원의 역할도 증가되고 있다. 현재 정신건강전문요원(mental health professionals)은 그 수가 14101명에 이르고 있지만, 보수 교육 제도와 개인 데이터베이스의 부재 등 자격에 대한 관리가 부실하여, 현황 파악 및 인력 수급 계획에 한계가 있다. 이에 수련현황과 전문요원의 실태에 대하여 조사하고 개선 방향을 제시하고자 하였다. 방 법 : 본 연구는 219개의 수련기관과 3396명의 정신건강전문요원으로부터의 설문 조사를 통하여 시행하였다. 결 과 : 경기와 인천 지역이 인구 10만명 대비 4.1, 3.9명으로 근무 인력이 가장 부족하였고, 전문 요원 중 유휴 인력의 81%는 복직을 희망 하고 있었다. 정신건강간호사의 경우, 최근 5년 동안 매년 600명에서 500여명으로 배출이 감소되고 있으며, 55% 이상의 정신건강임 상심리사는 치료기관 위주로 근무하고 있었다. 최근 법제화된 보수교육에 대하여는, 관련법과 정책, 정신질환의 이해, 윤리 교육에 대한 교육 수요가 높았다. 그리고, 전문요원 수련에 관하여 수련기관, 수련과정, 수련비용에 대한 보다 구체적 지침이 필요하였으며, 수련 과정에 대한 공통의 평가의 필요성도 제기되었다. 결 론 : 정신건강간호사 신규 인력의 증가와 정신건강임상심리사의 지역 사회로의 유입이 필요하겠다. 그 동안 필요성이 지속적으로 제기되어 왔던 전문요원 데이터베이스가 2018년에 사용 가능할 것으로 예상되므로, 보수교육과 더불어 향후 전문요원의 수급계획과 질 관리에 대한 초석이 될 것으로 기대된다. Objectives : Against the backdrop of reform of Mental Health Act in 2017, role of mental health specialists has been increasing in community mental health care. The number of mental health specialists has reached up to 14,101, but database for these human resources has not been built. Despite of many precedent researches, lack of database was a hurdle of accurate outcome of these studies. So, we did research on the training and current situation of mental health professionals. Methods : The authors analyzed reports from 219 training institutions and a web-based survey for the current situation and needs of 3,396 mental health professionals. Results : Gyeonggi province and Incheon showed 4.1 and 3.9 per 100,000 persons, the lowest in human resources per capita and 81% of idle manpower has a willing to be back to work. For the last 5 years, the number of new mental health nurses has decreased to 500 from 600. More than 55% of Clinical psychologists work at clinical setting. Continuing training programs were legislated and the highest need for the programs is about policy and law, psychiatric disorder, and ethics. There is a need for guideline for training institutions, training curriculum, and training tuition. Also, the examination for certification by council was suggested. Conclusion : Reinforcement of nurses and rearrangement of psychologists to community setting is needed. The reform of Mental Health Act could be a momentum of improvement of training programs for mental health professionals. Continuing training programs and database system on mental health professionals, which is available from 2018, would be expected to be a corner stone of human resources management plan for community mental health services.

Emulsion형 化粧品의 Escherichia coli 汚染防止에 대한 防腐制效果

柳美淑,金樟圭,元省皓,金男紀 成均館大學校 科學技術硏究所 1992 論文集 Vol.42 No.2

Most of cosmetics are emulsion products that contain the source of nutrition-vegetable oil, mineral oil and carbohydrate etc. -so there is much possibility of contamination by microbials. The purpose of this study is to get the data necessary not only to prevent dermatopathia occurred by Escherichia coli but to sustain the quality and the wear of cosmetics as the result of finding optimum growth condition and the growth condition of Escherichia coli for enviro-factor, utilizing antiseptic so as to prevent contamination by observing it in emulsion type cosmetic. The results are as follows : 1. The optimum growth condition of Escherichia coli : pH7.0 and 37.5℃ The mixing volume ratio of water / oil was 70/30 in this experiment. 2. Adding antiseptics into emulsion type cosmetic, the number of Escherichia coli was decreased from 10^8(cell/ml) to 8 x 10^3(cell/ml) 3. Antibacterial effect against Escherichia coli was better as follows, p-hydroxy benzoic acid propyl ester + phosphoric acid buffer solution > p-hydroxy benzoic acid buthyl ester + acetic acid buffer solution > p-hydroxy benzoic methyl ester + phosphoric acid buffer solution > p-hydroxy benzoic acid methyl ester + p-hydroxy benzoic acid buthyl ester >p-hydroxy

성교육 프로그램이 청소년 성의식과 성태도에 미치는 효과 : 정신병원 청소년병동 입원환자를 대상으로

류미숙 외 국립서울병원 정신보건교육·연구센터 2008 정신건강정책포럼 Vol.2 No.1

• Purpose : Sexual Education Program on adolescence sexual health has been developed by researchers in accordance with the national education courses. However, there have been not the study which found the effects of this program on sexual consciousness and sexual attitudes of psychiatric in-patient adolescents. • Method : Non-equivalent control group pretest-posttest design was used. Twenty six adolescents participated in an experiment. National Seoul mental hospital in-patients were selected for a convenience sampling method and have been taught the sexual education program during six weeks. • Result : There were significant differences in the sexual consciousness(t:-2.835. p:0.009) and sexual attitudes(t:-1.826. p:0.008) after the sexual education program • Conclusion : Since the sexual education program can affirmative influence on the sexual consciousness and sexual attitudes of the adolescence, it is recommendable to perform the sexual education program suitable for every stage of growth and development.

방부살균제에 의한 유화형화장품에서의 Staplylococcus aureus 의 성장억제

류미숙,김장규,김남기,원성호 한국산업안전학회 1992 한국안전학회지 Vol.7 No.3

S. aureus를 유화형 화장품시료에 접종배양한 결과, 최적성장조건인 pH 7.0, 온도 37℃에서 시료조성에 따른 성장률은 물과 오일의 부피비 조성에서 70 : 30, 80 : 20, 90 : 10, 30 : 70, 50 : 50의 순서로 균증식이 되었다. 물성에 있어 화장품시료의 pH는 40일 경과시 7.6에서 6.5로 변화되어고, 상대적으로 낮은 굴절률을 가는 물질들이 영양원으로 소모되어 시료(sample 3)의 굴절율이 1.4415에서 1.4490으로 변화되었으며, 균의 증식으로 인하여 균일하게 유화된 유화형 화장품시료의 相이 파괴되었고 약간의 변색, 변취와 함께 크리밍형상, 응집현상이 나타났다. 방부살균제가 첨가되지 않은 경우 약20일 경과후 부터 제품의 변질이 시작되었으나 방부살균제를 첨가하여 challenge test를 실시한 결과, S. aureus의 성장이 억제되었으며 40일 경과시 균수는 방부살균제가 첨가되지 않은 시료의 경우 10^8개/㎖이었으나 p-hydroxy benzoic acid propy ester - phosphric acid buffer solution을 200ppm 첨가한 경우 7×10^8개/㎖로 균수가 현저히 감소하였다. 균의 성장억제 효과는 p-hydroxy benzoic acid propylester+phosphoric acid buffer solution〉p-hydroxy benzoie acid methyl ester+phospheric acid buffer solution〉p-hydroxy benzoic acid methyl ester+phydroxy benzoic acid propyl ester〉p-hydroxy benzoic acid methyl ester+acetic acid buffer solution의 순으로 우수하였고 pH, 농도, 균수 등은 양호한 수준을 유지하였다. Emulsion-type cometics contain many kinds of carbon and energy source i.e., vegetable oil, mineral oil and carbohydrate etc., those can be used as nutrients and caused contamination by micrabials. Thereby we have to keep cosmetics from the possibility of contamination by microbials. From this viewpoint, the purpose of this study is to get the data necessary not only to prevent dermatopathia occurred by microbials but also to sustain the quality. In this experiment, we observed how many Staphylococcus aureus were grown in the prepared cosmetics with or without antiseptics so as to prevent contamination. When the contamination proceed, the stability of phase was disturbed and creaming phenomina was happened with some discoloration and bad smell. About 40 days after, the pH was changed from 7.6 to 6.5 and the refractive index of cosmetic raw materials were changed from 1.4415 to 1.4490(water : oil=70:30). By adding antiseptics into prepared cosmetics, the number of Staphylococcus aureus with challenge test method were decreased to 7×10^8 cell/㎖. For the antibacterial effect against Staphylococcus aureus, p-hydroxy benzoic acid propyl ester in phosphoric acid buffer solution was the best.

자발성 점막하 식도 박리 1예

김수철,김우식,김병호,이정일,최수철,김효종,박주철,장영운,이재영,류미숙,동석호,장린 대한소화기내시경학회 1998 Clinical Endoscopy Vol.18 No.2

A 55-year-old male was admitted to our hospital complaining of chest discomfort, dysphagia and severe pain on swallowing. Esophageal manometry identified the lower esophageal sphincter as having no response to swallow. Barium swallow showed typical esophagograms of double barrelled esophagus or mucosal stripe. An endoscopic examination revealed an appearance as if an esophago-tracheal fistula had been made at the level of the upper thoracic esophagus. Also, two slitlike mucosal tears were seen on the lower esophagus. Subsequent fasting and total parenteral nutrition for several weeks failed to bring about any improvement in his symptoms. So, as a treatment, primary closure of the upper opening of the false channel was performed under general anesthesia. Soon after the surgical procedure, the patients symptoms disappeared except for mild dysphagia. He was discharged after oral intake had been judiciously commenced with fluids and soft diets. During his follow-up in the out-patient department, he was examined and found to have no specific symptoms including fever or dysphagia. Moreover, there was great improvement from the massive dissection of the esophagus on esophagogram.

성장호르몬에서 뇌하수체 선종의 성장호르몬 방출호르몬 수용체 유전자의 발현과 내분비적 양성과의 관계

김진우,최영길,김영설,김선우,김성운,양인명,우정택,박승준,박철영,류미숙 대한내분비학회 1999 Endocrinology and metabolism Vol.14 No.2

Background: Growth hormone-releasing hormone (GHRH) plays a key role in the regulation of the proliferation and differentiation of somatomammotroph cells as well as secretion of GH. The actions of GHRH are mediated through the GHRH receptor (GHRH-R) that is a G protein coupled receptor with seven transmembrane domains. It has been demonstrated that alternative splicing occurs in the third cytoplasmic domain of rat and human GHRH-R mRNA, However, the clinical significance of the altemative splicing remains to be unsolved. To find an insight into the clinical significance, we investigate the correlation between the GHRH-R gene expression and a variety of clinical clinical and endocrinological findings in 11 acromegalic patients. Methods: Eleven acromegalic patients (3 males and 8 females, mean age 43.5 years) were included in this study. Six endocrine tests were carried out to evaluate the GH seeretory function of tumors. Invasiveness of tumors were evaluated by preoperative MRI findings on the basis of Hardys classification. Sequence the gsp oncogene and estimate the GHRH-R gene expression by RT-PCR and in vitro transcription. Results: Three different sized cDNA fragments, 250 bp, 700 bp and 810 bp, were found after RT-PCR. The amount of 250 bp fragment was higher than those of the other two fragments. The clinical findings (age, size, GH level, frequency of paradoxical response to TRH or GnRH, octreotide response, hypothalamic somatostatinergic activity) of the group with high expression of the 250 bp fragment did not significantly differ from those of the group with low expression. The GHRH-R gene expression of tumors with gsp oncogene did not significantly differ from that of tumors without gsp oncogene. Conclusion: These results suggest that the expression of GHRH-R gene may not be an important determinant for tumor growth, and the lower GH response to GHRH of tumors with gsp oncogene may not be attributed to the lower expression of GHRH-R gene. The expression of GHRH-R is likely to be regulated by a certain property of tumors for GH secretion and growth. (J kor Soc Endocrinol 14:241-254, 1999)