심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

서울지역 여대생들의 떡에 대한 인식 및 섭취 실태 조사

김영순,오순덕,김향미,신지홍,김현경,배유미,조규영,조성환 高麗大學校 倂設 保健大學 保健科學硏究所 2003 保健科學論集 Vol.29 No.1

This study conducted a survey of the improvement and popularization for Korea Rice Cakes, a Korean traditional food, on two hundred women students in five colleges in Seoul, Korea. It was investigated to get the information of the recognition, preference, and consumption patterns for the succession and improvement of Korean Rice Cakes. The results are as follows: 1. A portion of 41.5% among responders knows from three to six kinds of Korean Rice Cakes, and of 28% can make at least one among the kinds of Korean Rice Cakes. 2. A portion of 91% recognizes that Korean Rice Cakes are one of well-nourished food, but of 42% answers that Korean Rice Cakes cannot be replaced with the principle food. Also, there are no significant differences in the recognition of Korean Rice Cakes as the kinds of religions. 3. The frequency of purchase is about one or twice in a month (35.5%). Consumers (36.5%) intend to purchase Korean Rice Cakes for the commemoration of national holidays or anniversaries. 4. The reasons for the preference of other foods more than Korean Rice Cakes are the easiness of purchase (36.0%), the deliciousness (21.5%), the diverse kindness (17.5%), and the proper package (9.5%). It is important that constant and particular school / home education is need to improve and success Korean Rice Cakes.

항암화학요법 관련 오심과 구토 완화를 위한 비약물요법에 관한 연구동향

소향숙,최자윤,조인숙,김영재,김지영,김애숙,김옥미,김춘심,김현오,설영애,안정옥,이애리,이영자 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: Purposes of this study were to understand the current trends on complementary therapy in relieving chemotherapy-induced nausea and vomiting and to suggest the future research direction. Method: Subjects were selected on CINAHL, MEDLINE, Korean Academy Data Base from 1980 to 2001 which used nausea, vomiting, chemotherapy and complementary therapy as key words in experimental studies. Eight korean articles and twenty-one international articles were analyzed in terms of general characteristics, research methods, and types of complementary therapy. Data were analyzed by using descriptive statistical methods. Result: Since 2000, researchers have more actively used complementary therapy. In subject characteristics, mean age was 35.5 years old, 45% of the researchers were performed with high level of incidence of chemotherapy induced vomiting, 14% of them set limit of consecutive cycle during research, and 65% of them did not comment the selecting criteria of sample. About 60% of them were designed post-test only control group: 35% used INV by Rhodes, 31% used Likert scale, and 24% used VAS for dependent variable. Muscle relaxation therapy was mostly applied for relief of nausea and vomiting. Conclusion: Further studies will be needed to control extrinsic variables affecting nausea and vomiting in research design and to accumulate evidence with studies applying various complementary therapies.

포로 체험의 재구성과 기억의 공유

김미향 ( Kim Mi Hyang ) 아시아문화학술원 2017 인문사회 21 Vol.8 No.4

이 연구는 공적 기억인 역사 속에 숨어 있는 개인의 체험과 기억을, 문학을 통해 해석해 냄으로써 한국전쟁 포로 소설의 특징과 존재 방식을 재구해 내고자 했다. 이를 위해 Ⅱ장에서는 전쟁의 목적조차 잃은 채 과열된 갈등으로 폭력과 살육으로 폭주하는 포로들의 모습을 통해 이데올로기의 허구성을 증명하였고, Ⅲ장에서는 전쟁의 직접성에서 벗어나 일상이 전경화 된 상태에서 포로 체험의 기억을 타자와 공유하면서 공감을 불러일으키는 과정을 확인하였다. 포로들의 삶이 개인의 특별한 체험에 불과하다고 치부하거나 또는 `포로`라는 이름으로 이들의 삶을 일률적으로 규정짓지 않고 포로들 각각의 삶을 통해 그 함축된 의미를 고찰해낸다면 이데올로기 대결의 가장 중심에 놓인 핵심적 인물인 포로들이 전쟁으로 고통 받고 희생된 대표적 `비극적 삶의 주체`임을 증명하게 될 것이다. 그리고 이러한 과정을 통해 역사를 보는 시각은 더 다양해지고 우리 전쟁문학의 스펙트럼은 더욱 넓어질 것이다. In the situation tied to the immediacy of war, reorganization of repetitive captive experiences led to tragic ending by making captives` scars fixed. Also, fierce conflicts among captives in the prison camp resulted in cruel slaughter and violence, and Southern Government which had left them in the danger of murder and violence used them politically under the pretense of the release, never considering captives. Captives forgot the purpose of the war and rushed to the violence and slaughter without telling friends from enemies due to excessive conflicts among them, which obviously reveals unrealistic ideology. On the other hand, starting to share the memory of the captive experiences in a state that daily lives are in the foreground, the characters in Kim`s work who are free themselves from the immediacy of war arouse others` sympathy. However, whether the ending is tragic or reconciliatory, it has in common the fact that they are not courageous soldiers or vicious enemies who engaged in the battle field but are normal people and captives of Korean War. They show us that the violence in the prison camp wasn`t individual choice but inevitable result of the war, along with the tragic ending they chose. This makes us check that the captives, key characters placed in the center of ideology confrontation, may be actually representative victims by the ideology, all of whom were most irrelevant to the ideology.

도라지가 난소를 절제한 흰쥐의 혈 중 지질 함량 변화에 미치는 영향

김미향,김원희,박미라,한희선,배송자 신라대학교 자연과학연구소 2003 自然科學論文集 Vol.11 No.-

This study was designed to observe the effects of the feeding Platycodon grandiflorum A.DC(PG) extract on the improvement of the lipids on serum in ovariectomized rats. To investigate the effects of ethanol extract, Sprague-Dawley female rats were randomly assigned to groups as follows: Sham, OVX-control and ovariectomized rats supplemented with PG at 50㎎/㎏ bw/day. Body weight gain was not significantly different in groups. The levels of total cholestcrol was higher in ovaiectornized control rats than Sham-operated rats, but supplementation of PG ethanol extracts at 50mg/kg bw/day decreased the level of cholesterol in surum. These results might be expected that ethanol extract of PG is believed to be possible protective effects for the fatty serum increasing serum lipid.

미국과 일본 미술교육과정의 동향 및 내용에 관한 비교연구

김향미 서울교육대학교 초등교육연구소 1997 한국초등교육 Vol.9 No.2

The purpose of this study is to obtain fundamental materials on the curricular reform measures of art education by comparatively examining the tendency of educational curriculums between the USA and Japan, especially based on the historical curricular changes of elementary art education. The subject that is conclusively suggested is to establish a curricular direction in order to set its general thought and concretely achieve its goal. Through its accumulated achievements of the goal following the establishment of the curricular direction, more concrete theory would be provided and its achievements could be newly expected. A curriculum, a phenomenon situated in a border side between an ideal educational policy and actual educational state, needs to more flexible educational structure, not only by a planned theory as far as it's for human beings having a self-contradiction.

막걸리 농축액의 투여가 흰쥐의 혈중 지질 함량에 미치는 영향

김미향,김원희,배송자 가톨릭대학교 자연과학연구소 2001 自然科學論文集 Vol.9 No.-

동동주, 청맥 농축물의 투여가 흰쥐의 성장률, 혈청 효소활성 혈청 지질에 미치는 영향을 조사하였다. 실험동물의 체중 증가량은 동동주, 청맥 농축물의 농도별 투여가 대조군과 비슷한 경향을 나타내었으므로, 동동주, 청맥의 투여가 성장률에 지장을 주기 않음을 알 수 있었다. 총 콜레스테롤 함량, 중성지질 함향은 DD1군과 CM1군에서 대조군 보다 낮은 경향을 나타냈고, LDL함량은 DD1군에서 대조군 보다 낮은 경향을 나타냈다. 이러한 결과로부터 동동주 8g/kg 투여는 25%(v/v) 에탄올 5g/kg/day 경구 투여가 만성중독을 일으킬 수 있는데 반해 혈중 지질 함량을 감소시키므로 혈중 중성지질 농도 증가로 인한 심질환 예방에 기여 할 것으로 사료된다. Makkoli is a traditional and general alcoholic beverage in Korea. It's brewed from rice, shows an alcohol concentration of about 16%, has a characteristic fragrance, is slightly acidic, and has a sweet taste. Recently, taking these tradition alcoholic beverages are decreased. We examined the effect of Dongdongju and Cheongmaek kinds of Makkoli on serum enzyme activity and lipid concentration in rats. Sparague-Dawley rats were randomly assigned to groups as follows: control group, Dongdongju 8g/kg administered group(DD1), Dongdongju 1.6gkg administered group(DD2), Dongdongju 0.8g/kg administered group(DD3) and Cheongmaek 8g/kg administered group(CM1), Cheongmaek 1.6g/kg administered group(CM2), Cheongmaek 0.8g/kg administered group(CM3). Experimental diets were fed for 6 weeks. The body weight gain, seram enzyme activity and serum lipid values of all groups were compared with control group. There were no differences in growth. Alkaline phosphatase and GPT activities of DD3 and CM2 were lower than control group, but no significant differences. The total cholesterol concentrations of DD1 and CM1 were lower than that of control group. The triglyceride concentration of DD1 and CM1 was the same as results of total cholesterol concentration. These results suggest that Dongdongju and Cheongmaek positively influenced on serum lipid concentration.

일부 도시지역을 유배우여성들의 가족계획 실천정도에 관한 조사연구

김미향 순천청암대학 1989 論文集-順川看護專門大學 Vol.13 No.-

This survey aimed to grasp the actual practice of the family planning of married women in Urban areas. It was carried out through 400 numbers of random selected married women living in Jeonju City from July. 12, 1989 to August. 8, 1989. Questionnaire were used as the research means and the results were used as the research means and the results were analized by percentage and χ^2-test. The summarized results of this survey are as follows; 1. The average age at marriage of the group was 25.67years old, the average frequency of gravidity 4.16, and the number of their living chilgren 2.208. 2. The expieriences of Induced abortion were the average frequency of 1.157.(Age related to the Induced abortion p<.001^(***) level of significant difference) 3. 47.8% unwanted babies by reason of Induced abortion. 4. 68.5% of the group had the actual practice of contraception, and age, education level, religion and Occupation were not related to the significant difference) The most frequently used Contraceptive method was the Tubal Ligation 31.0%, Vasectomy 16.1%, Condoms 13.5%, Menstrual cycle method 13.5% and Oral Pill 6.6% were the next. 5. Regarding to the reasons for not using contraception "because of wanting to have more babies" commanded top ratio 42.1% and "worring about side reaction 19.O% were the next. 6. Most important route from which they knew about the Contraception was "Public health center. Hospital" commanded top ratio 43.3%. 7. 66.0% of the group knew about the correct meaning of family planning.

암환자의 원인지각 요인에 관한 연구

김유미,소향숙 성인간호학회 2001 성인간호학회지 Vol.13 No.4

The purpose of this study is to identify factors of the causal attribution of cancer and to determine related variables. Method: Subjects were one hundred and thirty three cancer patients. The tool of the perceived causal attribution used was developed by authors and basically founded on Kim's work(1993). The SAS program was used to analyze the data along with descriptive statistics. t-test. ANOVA. Duncan's Multiple range test, and Principal component analysis and varimax rotation. Results: 1) The perceived causal attribution measurement revealed four factors; overload, destiny, stress, and constitution. The total percentage of variance explained by the four factors was 44.3%. 2) The scores of destiny on women, having religion, unemployed, lower level of education, no spouse, groups of uterine cervix and lung cancer, not receiving an operation and receiving radiation were significantly higher than those other groups. 3) The scores of stress on women, having religion, and not having a job were significantly higher than those on men, without religion, and having a job. 4) The scores of constitution on those in their forties, women, not receiving an operation and receiving radiation were significantly higher than for those in their sixties, men, receiving operation and not receiving radiation. There was no significant difference in the factor scores of overload by any variables. Conclusion: Factors of the perceived causal attribution of cancer among Korean cancer patients were overload, destiny, stress, and constitution. The scores of each factor the perceived causal attribution was significantly different by general and disease related characteristics.

쑥이 흰쥐의 Collagen 합성에 미치는 효과

김미향 신라대학교 자연과학연구소 2004 自然科學論文集 Vol.13 No.-

폐경을 앞둔 여성에게는 폐경을 전후하여 지속되는 골 손실 가속화 등의 문제점을 가지고 있다. 그러므로 본 연구에서는 인위적 폐경을 유발시킬 수 있는 난소 절제 쥐에서의 골손실의 유무를 결합조직 중의 collagen 함량 변화를 통하여 그 효과를 검토하였다. 생화학적 골 대사 지표인 혈 중 ALP 활성은 난소 절제로(OVX-control)로 인하여 증가하는 반면, 난소절제 후 쑥 추출물 투여(OVX-AP)로 혈 중 ALP 활성은 감소하는 경향을 보여 골 흡수를 저하시킬 수 있는 가능성을 나타내었다. 한편 난소 절제 후 쑥 추출물 투여에 의해 피부 조직과 골·연골 조직에서 collagen 함량이 증가하는 경향을 보였고, 쑥 추출물 투여군 모두에서 collagen 합성량이 유의적으로 증가하였다. 쑥 추출물이 에스트로겐 감소로 인한 collagen 합성저하를 회복시킨 본 실험 결과로 미루어보아 쑥 중의 phytoestrogen이 에스트로겐 유사효과를 나타냄을 알 수 있었고, 외인성 에스트로겐 투여로 인한 부작용을 줄여 줄 수 있을 것으로 기대된다. 이상과 같이 폐경기 여성에게 쑥 추출물의 섭취는 폐경으로 인한 장애를 감소시키기 위하여 에스트로겐의 장기간 복용에 의한 부작용을 쑥 중의 phytoestrogen이estrogen 대체 작용을 함으로써 그 부작용을 감소시켜 줄 것으로 사료되며, 이들의 구조 및 기전에 대해서는 앞으로 연구가 더 필요한 것으로 생각된다. The aim of this study was to evaluate the effects of Artemisia Princeps Var. Orientalis (AP) on the collagen content of the connective tissues in ovariectomized rats. From day 2 until day 37 after the ovariectomy, Sprague-Dawley female rats were randomly assigned to the following groups : sham-operated rats (Sham), ovariectomized control rats (OVX-control), and ovariectomized rats supplemented with the AP 50 mg/kg bw/day (OVX-AP50). The AP ethanol extracts were orally administrated 1 mL per day. The OVX rats were significantly heavier than the sham-operated rats at all time points, but supplementation with the AP extracts tended to gain weight less than OVX-control. Supplementation with the AP extracts prevented a decrease in the collagen level in bone and cartilage tissues. These results are consistent with the conclusions based on the estrogenic activities of AP. Therefore, it may be used to possibly improve the quality of life in menopausal women.