PCR 기법을 이용한 한국재래산양 β-casein 유전자의 특성

김지애,류승희,유성란,이준헌,서길웅,김선균,상병찬 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.2

본 연구는 한국 재래 산양 112두와 유산양인 Saanen종 7두의 혈액으로부터 genomic DNA를 추출하고, PCR-RFLP 방법에 의해 β-casein 유전자의 특성을 분석하여 한국재래산양의 효율적인 유전자원의 보전 및 개량을 위한 기초 자료로 제공하고자 실시하였다. 한국재래산양의 genomic DNA로부터 PCR기법을 이용하여 β-casein의 유전자좌를 증폭한 결과 각각 481bp 크기의 단편이 양호하게 증폭되었음을 확인하였다. β-casein 유전자좌의 증폭산물에 대한 Bal Ⅰ의 제한효소를 처리한 결과, β-casein AB형은 481bp, 284bp 및 197bp의 단편을, 그리고 BB형은 284BP와 197BP의 단편을 한국재래산양과 유산양인 Saanen 종에서 확인 할 수 있었다, 유전자형 빈도에 있어서는 한국재래산양에서 β-casein AB 및 BB의 빈도는 각각 6.25 및 93.75%이었고, 유산양인 Saanen 종은 각각 57.14 및 42.86%이었다. 유전자빈도에 있어서는 한국재래산양의 β-casein A 및 B의 빈도가 각각 0.031 및 0.969이었고, Saanen 종에서는 각각 0.286 및 0.714의 빈도를 보였다 한국재래산양의 β-casein 유전자의 염기서열과 이미 보고되어 있는 goat의 염기서열(GeneBank accession Number M90556)간에는 총 11개의 염기서열에 차이를 나타내어 97.71%의 상동성을 보였다, 따라서 한국재래산양의 β-casein 유전자의 다형성과 염기서열 분석에 의한 분자유전학적 특성의 규명은 한국재래산양의 유전자원의 보전 및 개량을 위한 기초 및 응용 자료로 이용될 수 있을 것으로 생각된다. This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from analysis of genetic characteristics of β-casein gene in Korean Native goat and Saanen using the PCR-RFLP. This study confirmed the amplified products of 481bp fragments obtained from the amplification of β-casein loci by PCR. The β-casein AB genotype showed 481, 284 and 197bp, and β-casein BB genotype showed 284 and 197bp fragments in Korean Native goat and Saanen. The frequencies of β-casein genotype in Korean Native goat were 6.25 and 93.75% for AA and AB and the frequencies of β-casein genotype in Saanen were 57.14 and 42.86% for AA and AB types. The frequencies of β-casein A and B alleles were 0.031 and 0.969 in Korean Native goat and the frequencies of β-casein A and B alleles are 0.286 and 0.714 in Saanen, respectively. The nucleotide sequence of β-casein gene of Korean Native goat was 97.71% higher homology with 11 nucleotide sequences difference of that of goat reported in GeneBank (M90556). Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphism and sequencing for β-casein gene should be used as basic and applying data for preservation and improvement of genetic resources in Korean Native goat breeding.

중증 화상 환자에서 영양지원

박지영,목혜숙,신현선,노순예,김애심,백금선,김도헌,허준,전욱 대한화상학회 2003 대한화상학회지 Vol.6 No.2

복막투석복막염 환자에서 발생한 창자벽공기증 1예

정선영,나지훈,최윤정,고성애,조규향,박종원,도준영,윤경우 영남대학교 의과대학 2009 Yeungnam University Journal of Medicine Vol.26 No.1

Peritonitis is a serious problem in patients undergoing peritoneal dialysis. Rarely pneumatosis intestinalis can occur as a complication of this infectious process. Pneumatosis intestinalis is a potential life threatening condition with a challenging management. The mortality of peritoneal dialysis patients with pneumatosis intestinalis secondary to mesenteric ischemia is almost 100%. We describe a rare case of pneumatosis intestinalis in a peritoneal dialysis patient who developed Staphylococcus aureus peritonitis which was initially treated with appropriate antibiotics. Since initial response to therapy was not achieved, an abdominal computerized tomography was done which revealed a pneumatosis intestinalis. A laparotomy was performed and small bowel necrosis was seen. A segmented resection with ileostomy, jejunostomy was done. Though surgical treatment was performed, the patient died in 2 weeks after admission. Pneumocystitis intestinalis in peritoneal dialysis peritonitis is a uncommon complication which requires prompt evaluation to rule out mesenteric ischemia as it carries a high mortality and its management will be surgical.

정신분열병의 유전적 고위험군에서 인지, 정서 및 사회기능 연구

이경진,위휘,유소영,이애라,송지연,하태현,홍경수,김명선,권준수 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.1

Objectives : Vulnerability marker in biological relatives of schizophrenia may be very useful in identifying the propensity for the development of schizophrenia. This study suggests that the relatives at risk for genetically developing schizophrenia have impairments in several domains of cognition, social function and affective function as a vulnerability marker. Methods : A neurocognitive function test, a social function test and an affective function test were administered to fifteen healthy relatives from families with two or more patients with schizophrenia (geneticallly high risk subjects). Fifteen healthy controls matched for age and gender were recruited in this study. Results : Compared to the control subject, the genetic high risk subjects performed significantly more poorly in the selective attention and recall memory of neurocognitive function. The high dsk subjects also had lower scores in independence-performance and independence-competence of the social function test and emotion control of the affective function test. Conclusion : Selective attention, recall memory, independence-performance, independence-competence and emotion control ability may be a valuable marker for genetic study of schizophrenia.

정신분열병의 임상적 고위험군의 특징 : '서울청년클리닉'의 자연 추적 관찰 예비연구

유소영,이경진,강도형,이승재,하태현,위휘,이애라,송지연,김성년,권준수 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.1

Objectives : The aims of this study were to identify the intake and following process for subjects at high risk of transition to schizophrenia and to examine the neurocognitive, social, and emotional functions of the subjects compared with healthy controls. Methods : Symptomatic individuals judged at high risk for schizophrenia ('clinical high risk') within Youth Clinic were assessed and followed up. They performed a neurocognitive function test, a social function test and an affective function test. Twenty healthy controls were recruited in this study. Results : Among eighty-two subjects contacted through the Youth clinic, sixteen subjects were judged as the clinical high risk group. Fourteen subjects among the clinical high risk group showed deficits in several domains of neurocognitive functions, such as visual recall memory, verbal short term memory and executive function. Social and affective functions are also impaired in the Cli-nical high risk group compared with healthy controls. Two of 15 subjects (13%) developed a psychotic disorder within 6 months. Conclusion : This study illustrates the state of follow-up study for a clinical high risk group. Despite low numbers and short durations, some impairment of several functions in the clinical high risk group suggests possible predictors of psychosis.

Analysis of increased nuchal translucency: Chorionic villi sampling and second-trimester level II sonography

Ji Eun Park,Ji Kwon Park,In Ae Cho,Jong Chul Baek,Min Young Kang,Jae Ik Lee,Jeong Kyu Shin,Won Jun Choi,Soon Ae Lee,Jong Hak Lee,Won Young Paik 대한의학유전학회 2014 대한의학유전학회지 Vol.11 No.2

Purpose: To assess the outcomes of increased fetal nuchal translucency (NT), to aid in prenatal counseling and management in our practice. Materials and Methods: We retrospectively reviewed the medical records of patients who underwent first trimester fetal karyotyping using chorionic villi sampling (CVS) and second trimester level II sonography for a fetal NT thickness ≥3.0 mm between 11 weeks and 13 weeks 6 days' gestation, at Gyeongsang National University Hospital. Pediatric medical records and a telephone interview were used to follow-up live-born children. Exclusion criteria included incomplete data and CVS for other indications. Results: Seventy cases met the inclusion criteria (median NT thickness, 4.7 mm; range, 3.0-16.1 mm). Twenty-nine cases (41.4%) were aneuploid. The prevalence of chromosomal defects increased with NT thickness: NT 3.0-3.4 mm, 16.7%; NT 3.5-4.4 mm, 27.3%; NT 4.5-5.4 mm, 66.7%; NT 5.5-6.4 mm, 37.5%; NT ≥6.5 mm, 62.5%. The most common karyotype abnormality was trisomy 18 (n=12), followed by trisomy 21 (n=9). In chromosomally normal fetuses (n=41), fetal death occurred in 2 cases (4.9%), and structural malformations were found in 11 cases (26.8%). In chromosomally and anatomically normal fetuses (n=28), one child had neurodevelopmental delay (3.6%). Twenty-eight infants who had a prenatal increased NT were alive and well at follow-up (40%). Conclusion: Outcomes of increased fetal NT might help inform prenatal counseling and management. The high prevalence of chromosomal defects associated with increased fetal NT implies that CVS should be performed in the first trimester, particularly considering the stress associated with an uncertain diagnosis.

OB-33 : The effect of early third trimester emergency cervical cerclage on pregnancy outcome

( Ji Hye Lee ),( Ji Eun Park ),( Jeong Kyu Shin ),( Won Jun Choi ),( Soon Ae Lee ),( Jong Hak Lee ),( Won Young Paik ),( Ji Kwon Park ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

To evaluate the effectiveness of early third trimester (26-30 weeks gestation) emergency cervical cerclage on pregnancy outcome in women with cervical incompetence. Medical records were reviewed for demographic and clinical data, gestational age at cerclage, functional cervical length after cerclage, cerclage-delivery interval, gestational age at delivery, and neonatal birth weight. Forty-one patients underwent emergency cervical cerclage between 16-30 weeks gestation. Sixteen patients underwent cerclage at 16-21 6/7 weeks (Period I); 12 patients, at 22-25 6/7 weeks (Period II); and 13 patients, at 26-29 6/7 weeks (Period III). There were no significant differences among Periods I, II, and III with respect to functional cervical length after cerclage (2.6 ± 0.6, 2.4 ± 0.3, and 2.3 ± 0.5 cm, respectively), cerclage-to-delivery interval (71.7 ± 54.2, 69.2 ± 36.3, and 55.5 ± 25.2 days, respectively), gestational age at delivery (30 2/7 weeks ± 59 days, 34 5/7 weeks ± 36 days, and 35 3/7 weeks ± 30 days, respectively), rate of deliveries after 28 weeks (56.2, 83.3, and 84.6%, respectively), rate of deliveries after 32 weeks (50.0, 75.0, and 84.6%, respectively), rate of deliveries after 36 weeks (37.5, 58.3, and 53.8%, respectively), or neonatal birth weight (1871 ± 1473, 2588 ± 1006, and 2678 ± 944 g, respectively). Gestational age at emergency cerclage and gestational age at delivery were significantly correlated (p=0.001, R2 = 0.24). These results address the benefits of attempting emergency cerclage during the early third trimester.

Effect of Paquinimod on Neutrophilic Inflammation of Murine Asthma Model

( Ji Ae Jun ),( Da-jeon Bae ),( Tae-hyeong Lee ),( Hun Soo Chang ),( Jong Sook Park ),( Choon-sik Park ) 대한결핵 및 호흡기학회 2018 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.126 No.-

Background: S100A9 is known to exert pro-inflammatory effects in various chronic inflammatory disorders. We previously demonstrated the elevation of S100A9 in the sputum of neutrophilic inflammation in severe uncontrolled asthma and neutrophilc asthma model. The purpose of this study was to evaluate the effect of Paquinimod on the neutrophilic inflammation of murine asthma model. Materials and Methods: For neutrophilic inflammation model, mice were I.P. sensitized with OVA/CFA and challenged by I.N. OVA and does dependent of paquinimod with drinking water. BAL and histological assessment on inflammation were performed after the measurement of Airway resistance using a flexiVent. Lung tissue sections were stained with periodic acid-Schiff staining to count goblet cell. Levels of S100A9, Caspase-1, IL-1β were measured in the lung using western blotting. S100A9 and neutrophil elastase expression in the bronchus cells was assessed by using confocal microscopy. Result: Total cell and neutrophil were increased in the OVA/CFA mice and paquinimod suppressed the cell numbers in murine model. Concomitantly, the enhanced airway resistance of the OVA/CFA mice was attenuated. In histology, proportion of goblet cells in bronchus were markedly decreased by the treatment with paquinimod. On western blot, Paquinimod treatment significantly decreased the enhanced expression of S100A9, Caspase-1, IL-1β in CFA/OVA mice. Conclusion: Paquinimod may be an effective inhibitor on neutrophilic asthma of murine model via inhibition of inflammatory mediators and IL-17. (Fund code 2017-R1A2B40126)

Activation of the Nrf2 signaling pathway and neuroprotection of nigral dopaminergic neurons by a novel synthetic compound KMS99220

Lee, Ji Ae,Son, Hyo Jin,Choi, Ji Won,Kim, Jinwoo,Han, Se Hee,Shin, Nari,Kim, Ji Hyun,Kim, Soo Jeong,Heo, Jun Young,Kim, Dong Jin,Park, Ki Duk,Hwang, Onyou Elsevier 2018 Neurochemistry International Vol.112 No.-

<P><B>Abstract</B></P> <P>The transcription factor Nrf2 is known to induce gene expression of antioxidant enzymes and proteasome subunits. Because both oxidative stress and protein aggregation have damaging effects on neurons, activation of the Nrf2 signaling should be beneficial against neurodegeneration. In this study, we report a novel synthetic morpholine-containing chalcone KMS99220 that confers neuroprotection. It showed high binding affinity to the Nrf2 inhibitory protein Keap-1 and increased nuclear translocation of Nrf2 and gene expression of the antioxidant enzymes heme oxygenase-1, NAD(P)H:quinone oxidoreductase-1, and the catalytic and modifier subunits of glutamate-cysteine ligase in dopaminergic CATH.a cells. KMS99220 also increased expression of the proteasome subunits PSMB5, PSMB7, PSMB8 and PSMA1, and the respective chymotrypsin and trypsin-like proteasomal enzyme activities, and reduced α-synuclein aggregate in GFP-α-syn A53T-overexpressing cells. KMS99220 exhibited a favorable pharmacokinetic profile with excellent bioavailability and metabolic stability, did not interfere with activities of the cytochrome p450 isotypes, and showed no apparent in vivo toxicity when administered up to 2000 mg/kg. In 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated mice, oral administration of KMS99220 prevented degeneration of the nigral dopaminergic neurons, induced the Nrf2 target genes, and effectively prevented the associated motor deficits. These results suggest KMS99220 as a potential candidate for therapy against Parkinson's disease.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We synthesized a novel compound KMS99220 that activates the Nrf2 signaling pathway. </LI> <LI> KMS99220 elevates Nrf2-dependent gene expression of HO-1, NQO1 and GCLM/GCLC. </LI> <LI> It raises expression and activities of proteases and lowers α-synuclein aggregation. </LI> <LI> It prevents nigral dopaminergic neurodegeneration and motor deficits in MPTP mice. </LI> <LI> It exhibits favorable pharmacokinetics, bioavailability and metabolic stability. </LI> </UL> </P>

Case Report : Torsion of a parasitic myoma that developed after abdominal myomectomy

( In Ae Cho ),( Jong Chul Baek ),( Ji Kwon Park ),( Dae Hyun Song ),( Wan Ju Kim ),( Yoon Kyoung Lee ),( Ji Eun Park ),( Jeong Kyu Shin ),( Won Jun Choi ),( Soon Ae Lee ),( Jong Hak Lee ),( Won Young 대한산부인과학회 2016 Obstetrics & Gynecology Science Vol.59 No.1

Iatrogenic parasitic myomas are rare. The condition is defined by the presence of multiple smooth-muscle tumorous nodules in the peritoneal cavity. This may be attributable to seeding of myoma particles during uterine surgery. The clinical course is usually indolent. The disease is often asymptomatic and is usually discovered only incidentally. A 38-year-old woman who had undergone abdominal myomectomy 7 months prior presented with acute abdominal pain and a huge pelvic mass. We performed exploratory laparotomy. A parasitic mass 17 cm in diameter with a twisted omental pedicle was identified. En bloc excision of the mass and omentum was performed, followed by total abdominal hysterectomy. Histopathological examination of multiple sections revealed features compatible with an infarcted leiomyoma. Thus, we present a very rare case of an iatrogenic, rapidly growing parasitic myoma complicated by omental torsion (which caused the acute abdominal pain). We also offer a literature review.