초음파 기구에 의한 치근단 와동형성후 역충전의 밀폐성에 대한 연구

이형일,손호현 大韓齒科保存學會 1993 Restorative Dentistry & Endodontics Vol.18 No.1

AbstractThis study was conducted to evaluate and compare the apical leakage in the following retrofil- ling techniques after apical resection ; No apical cavity preparation and no retrofilling(control group), Amalgam(group I) or silver glass ionomer cement(group II) retrofilling after apical cavity preparation with mini contra-angle and bur, Amalgam(group III) or silver glass ionomer cement(group IV) retrofilling after apical cavity preparation with ultrasonic micro endo tip.Extracted ninety upper anterior and lower canine teeth were fixed in skull simulators and root canals were prepared with step-back method and obturated with gutta-percha and zinc oxide eugenol sealer. Obturated roots were resected 2mm from apical ends and apical cavities of 1mm width and 2mm depth were prepared and retrofilled by above mentioned methods.After application of nail varnish on all surface except resected surface, apical 1/3 of the roots were placed in 1% methylene blue solution for 3 days. After longutudinal sectioning to expose central parts of filled materials, depths of penetrated dye were measured by measu- ring microscope and were analyzed statistically.The results were as follows. 1. Having no relation with instruments used in apical cavity preparation, amalgam retrofilling groups(group I and II) showed less apical leakage which was not significant statistically than no retrofilling group(control group) (P<0.05), but silver glass ionomer cement retrofi- lling gruoups(group II and W) showed significantly less apical leakage than no retrofilling group(control group) (P<0. Ol). 2. In the groups retrofilled with the same material, the apical leakage in cavities prepared with ultrasonic micro endo tip(group III and IV) was less than that in cavities prepared with mini contra-angle and bur(group I and II), but not significant statistically (P>0.05). 3. When apical cavities were prepared with same instrument, the egroups retrofilled with silver glass ionomer cement(group II and IV) showed significantly less apical leakage than the groups retrofilled with amalgam (group I and III.).

제지슬러지의 토지주입에 관한 연구

성낙창,김정권,손희정,김은호,김형석 東亞大學校生命資源科學大學附設 農業資原硏究所 1996 農業生命資援硏究 Vol.5 No.1

To examine the agricultural availability of paper sludge, this study investigated the degradation rate of sludge, CO₂ generation out of soil treated with sludge and the degration characteristics of sludge in soil 1. Degradation rate of paper in soil at weeks after treatment was 19.0% at natural temperature and 28.0% at incubation temperature(30℃) 2. The changes of T-C, T-N and C/N ratio of paper sludge in soil at 12 weeks after treatment were 15.5%, 0.22% and 71, respectively, at natural temperature, and 14.5%, 0.24% and 60, respectively at incubation temperature. 3. The changes of pH in soil treated with paper were 6.7∼7.4 at natural temperature and 6.1∼8.0 at incubation temperature. 4. CO₂ generations in soil treated with 1.0%, 3.0% and 5.0% of paper sludge at 12weeks after treatment were 247mg/100g, 334mg/100g and 458mg/100g, respectively, at natural temperature and 385mg/100g, 550mg/100g and 618mg/100g, respectively, at incubation temperature(30℃)

국산 체외 충격파 쇄석기의 음향학적 특성 평가

최민주,이종수,김성삼,조성찬,양형석,손종수,천원기 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

In the study we measured and evaluated the acoustical property of a domestic spark gap type extracorporeal shock wave lithotripsy (ESWL). Altering the discharging medium between the electrodes of the shock wave generator (water, 1% an 10% NaCl electrolyte), we measured shock waveforms and sound pressure level (SPL). For the shock waves produced using the discharging medium, water, it was seen that the shock wave peak pressure went up to 70 MPa, and the SPL was in the range of 90 - 100 dB. As the concentration of NaCl increased in the electrolyte. the SPL did not change much and was shown to increase correlation with the discharging voltage. In the case of the discharging medium. 10% NaCl electrolyte, it was found that the shock amplitudes varied in a narrow range when being high in the concentration of NaCl and were large when being high in discharging voltages. While the stone fragmentation was in process, we measured the acoustic emission and compared their spectral properties.

당뇨병환자의 후각에 대한 영향인자의 분석

박시영,구한얼,구수권,이상화,손성표,장형준,노환중 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

Micro cell array on silicon substrate using graphene sheet

Son, Hyeong-Guk,Oh, Hong-Gi,Park, Young-Sang,Kim, Dae-Hoon,Lee, Da-Som,Park, Woo-Hwan,Kim, Hyung Jin,Cho, Seung-Min,Lim, Ki Moo,Song, Kwang Soup Elsevier 2017 Materials letters Vol.196 No.-

<P><B>Abstract</B></P> <P>To fabricate micro-patterns for bioengineering applications, we used graphene sheet, metal mask, and plasma treatment rather than the commonly used photolithography process. Two types of micro-patterns were fabricated (line, and circle) on SiO<SUB>2</SUB>/Si (100, p-typed) substrate. In the line and circle micro-patterns, graphene etched areas were 100 and 150μm, respectively, with fluorinated graphene spacing. The efficiencies of early cell adhesion, which is necessary for the growth and proliferation of cells, were 62, 17, and 65% on the pristine, fluorinated, and etched graphene surface, respectively, for 6h of cell culture. After seeding the neuron cells on the patterned substrate, neuron cells proliferated and differentiated along the graphene etched regions. The graphene sheet was used as a passivation layer for micro-array of the neuron cell on SiO<SUB>2</SUB>/Si.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Graphene sheet has been used in the miniaturization process. </LI> <LI> Graphene sheet was partially etched by plasma treatment in O<SUB>2</SUB> gas environment. </LI> <LI> Graphene sheet was fluorinated by plasma treatment in C<SUB>3</SUB>F<SUB>8</SUB> gas environment. </LI> <LI> Fluorinated graphene sheet artificially control the adhesion of cell. </LI> <LI> Fluorinated graphene sheet was used as a passivation layer for micro-cell array. </LI> </UL> </P>

Cathepsin B Inhibitor, E-64, Affects Preimplantation Development, Apoptosis and Oxidative Stress in Pig Embryos

Son, Hyeong-Hoon,Min, Sung-Hun,Yeon, Ji-Yeong,Kim, Jin-Woo,Park, Soo-Yong,Lee, Yong-Hee,Jeong, Pil-Soo,Koo, Deog-Bon The Korean Society of Animal Reproduction 2013 Reproductive & developmental biology Vol.37 No.4

Cathepsin B is abundantly expressed peptidase of the papain family in the lysosomes, and closely related to the cell degradation system such as apoptosis, necrosis and autophagy. Abnormal degradation of organelles often occurs due to release of cathepsin B into the cytoplasm. Many studies have been reported that relationship between cathepsin B and intracellular mechanisms in various cell types, but porcine embryos has not yet been reported. Therefore, this study evaluated the effect of cathepsin B inhibitor (E-64) on preimplantation developmental competence and quality of porcine embryos focusing on apoptosis and oxidative stress. The expression of cathepsin B mRNA in porcine embryos was gradually decreased in inverse proportion to E-64 concentration by using real-time RT-PCR. When putative zygotes were cultured with E-64 for 24 h, the rates of early cleavage and blastocyst development were decreased by increasing E-64 concentration. However, the rate of blastocyst development in $5{\mu}M$ treated group was similar to the control. On the other hand, both the index of apoptotic and reactive oxygen species (ROS) of blastocysts were significantly decreased in the $5{\mu}M$ E-64 treated group compared with control. We also examined the mRNA expression levels of apoptosis related genes in the blastocysts derived from $5{\mu}M$ E-64 treated and non-treated groups. Expression of the pro-apoptotic Bax gene was shown to be decreased in the E-64 treated blastocyst group, whereas expression of the anti-apoptotic Bcl-xL gene was increased. Taken together, these results suggest that proper inhibition of cathepsin B at early development stage embryos improves the quality of blastocysts, which may be related to not only the apoptosis reduction but also the oxidative stress reduction in porcine embryos.

Cathepsin B Inhibitor Affects Developmental Competence, Apoptosis and Oxidative Stress in Pig Embryos

Hyeong-Hoon Son,Sung-Hun Min,Enok Lee,Humdai Park,Deog-Bon Koo 한국동물생명공학회(구 한국동물번식학회) 2012 발생공학 국제심포지엄 및 학술대회 Vol.2012 No.1

Micropatterned Cell Array on Silicon Substrate

Hyeong-Guk SON,Dae-Hoon KIM,Min-Hye KIM,Da-Som LEE,Young-Sang PARK,Hong-Gi OH,Hyo-Geun NAM,Kwang-Hwan JHEE,Kwang-Soup SONG 한국생물공학회 2014 한국생물공학회 학술대회 Vol.2014 No.4

The solid-state fermentation of <i>Artemisia capillaris</i> leaves with <i>Ganoderma lucidum</i> enhances the anti-inflammatory effects in a model of atopic dermatitis

Son, Hyeong-U,Lee, Seul,Heo, Jin-Chul,Lee, Sang-Han UNKNOWN 2017 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.39 No.5

<P><I>Artemisia capillaris</I>, which belongs to the Asteraceae family and the genus <I>Artemisia</I>, has been reported to exert inhibitory effects on diabetes, cancer and inflammation. In this study, in order to enhance the bioactivity potential of the leaves of <I>Artemisia</I> by <I>Ganoderma lucidum</I> mycelium, we prepared aqueous samples of <I>Artemisia capillaris</I> (<I>Ac</I>) leaves, <I>Ganoderma lucidum (Gl</I>) and aqueous fractions produced by the solid fermentation of <I>Ganoderma lucidum</I> on <I>Artemisia capillaris</I> leaves (<I>afAc/Gl</I>). Thereafter, we evaluated whether these samples have potential to attenuate inflammation-related symptoms in an amimal model of 2,4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis. We found that <I>afAc/Gl</I> exhibited enhanced anti-inflamamatory activity following the solid fermentation process when compared with <I>Ac</I> or <I>Gl</I> on ear thickness, ear epidermal thickness and eosinophil infiltration in the skin tissues. The expression of nitric oxide (NO) synthases (NOSs) was measured by immunohistochemical staining. The results revealed that <I>afAc/Gl</I> decreased endothelial NOS and inducible NOS expression compared with the DNFB group, while neuronal NOS expression was not altered. By comparing NO production, we found that as opposed to <I>Ac, afAc/Gl</I> has potential to inhibit atopic dermatitis-related symptoms during the inflammatory event. As regards matrix metalloproteinase (MMP) expression patterns, <I>afAc/Gl</I> exerted potent inhibitory activity on the mRNA expression of MMP-2, -7, -9, -12, -14 and -19. Taken together, these results suggest that the solid state fermentation of <I>Ac</I> by <I>Gl</I> is an effective strategy to obtaining useful ingredients which are converted into valuable compounds during an atopic inflammatory insult.</P>