서남해안 지방에서 발생한 Vibrio vulnificus 감염의 임상적 고찰

조남수,김춘호,김성중,김용배,조수형,안태훈 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: V. vulnificus infection in found between May and October when the man who has hepatic disorder or immunity disorder eats raw shellfish and it causes the systemic symptoms such as sudden fever, chilling, shock and stupor and local skin lesions like bleb and necrosis. This disease has 46∼61% of fatality rate in spite of intensive treatment. This study conducts the examination of history, epidemic study, ingesting raw fish and bacteriologic investigation in the patients with similar clinical symptoms to the above and examines the diagnostic dorrelations. Method: It is conducted with 31 cases who admitted at emergency medical center of Chosun University Hospital from May of 1995 to September of 1998 and are suspected to have V.vulnificus infection. It analyze bacteriologic examination, the survey of residence, eating raw shellfish, hepatic disorder, alcohol drinking habits and skin lesion. Results: 1) The rate of male and female is 15.5:1 and in the age, 18 cases are in fifties, seven are in forties and six are over sixties. 2) Clinically, V.vulnificus is detected in 20 of 31 cases which is suspected to have V.vulnificus infection and it is not detected in 5 cases. Other bacteria are detected in 6 cases and they include staphylococcus aureus, pseudomonas species, β-hemolytic streptococcus and E-coil. 3) In local distribution, Kohung has 10 cases, Shinan and Mokpo have six, Haenam and Kangjin have five, Yongkwang and Muan have five, Naju and Yongam have five and Kwangju has one. 4) Eating fishes and raw shellfishes is found in 31 cases. In the kind of shellfishes, eating raw clam is 10 cases, raw thin-shelled surf calm is 8 cases, raw oyster is 5 cases and sliced raw fish is 4 cases and pickled sea foods are 4 cases. 5) The cases with hepatic disorder are 23 and 15 cases with heavy alcohol drinking Carrier are included. The cases with Diabetic Mellitus are four and those with pulmonary tuberculosis are two. Two cases have not basal diseases. 6) On the opinion of skin lesion, 20 cases show vesicular necrosis, 7 cases have dendriform erythema, 3 cases have papule and one case doesn't show clear skin lesion. Conclusion: When V.vulnificus infection is detected, it is fatal and those who have hepatic disorder or heavy alcohol drinking habit must avoid eating fishes and shellfishes raw in summer. It is important to have emergency treatment for the patient whose V.vulnificus infection is suspected. It is considered that the administration of initial antibiotics is effective by generalizing the clinical symptom, epidemic opinion and patient history even before the results of bacteriologic confirmed.

전방 또는 전측방 흉수 종양 수술에서 후측방도달법

조경석,박춘근,성재훈,허필우,박춘근,백민우,김달수,강준기 대한신경외과학회 1997 Journal of Korean neurosurgical society Vol.26 No.5

직업성질환 감시체계의 계획과 관리

조수헌,홍윤철,임종한,장성실,천병철 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.1

직업성 질환에 관련된 다양한 진료를 체계적으로 수집하고 관리하여 직업성질환의 예방 목적으로 이를 활용하고 자하는 노력이 우리나라에서도 이제 다양한 방법으로 시도되고 있다. 최근의 자료 시스템의 구축과 관리 방법의 향상은 새로운 직업성 잘환감시방법을 개발하게 하여 직업성질환과 손상을 예 방하는데 크게 기여하고 있다. 우리나라에서도 기존의 산업보건사업의 성과를 계승하면서, 외국의 직업성질환 감시의 여러 경험을 비판적으로 수용하여 한국적인 직업성질환 감시 모델을 구축해야 할 것으로 생각된다. 자발적인 지역차원의 직업성질환 감시체계들이 틀을 잡아가고 있는 이 시점에서 본격적으로 직업성질환 감시체계 수립을 위한 전략과 구체적 방법론을 마련하고 공유하여야 할 필요가 있다. 이를 위하여 우리나라의 직업성질환 감시체계를 구축하는데 기초가 될 수 있는 직업성질환 감시체계의 계획과 관리에 대한 내용을 정리하였다. 이러한 감시체계를 우리나라에 정립하려는 노력들의 일환으로 최근 직업성질환감시연구회의 결성 및 각 지역의 직업성질환 감시체계의 운영 등이 조금씩 결실을 맺어가고 있어 간략히 소개하면 다음과 같다.첫째, 최근에 인천, 천안, 대전, 여천, 구미 등에서 지역단위의 직업성질환 감시체계를 구축하고 있고 한국산업안전공단에서는 특수건강진단과 작업환경측정 자료를 전산화하여 이를 직업성질환 감시에 사용하는 전국단위의 감시체계와 천식, 근골격계질환, 피부질환 등 질환별 감시체계 등이 시도되고 있다.둘째, 이러한 움직임은 과거에 비하여 한층 발전된 양상이고 바람직하지만 이제는 국가적 차원에서 직업성질환 감시체계를 어떻게 설정하고 조율할 지를 고민하고 계획하여야 할 때이다. 즉 한국산업안전공단과 지역단위 직업성질환 감시체계들의 역할분담과 상호 정보의 교류 등이 정리되어야 이를 바탕으로 유기적인 국가 직업성질환 감시체계가 구축될 수 있다.셋째, 이를 위하여서는 직업성질환 감시체계 구축의 방법론을 서로 교류하고 공유할 필요가 있으며 나아가서는 이를 표준화하여 기본적인 자료의 교류가 원활하게 이루어질 수 있도록 하여야 한다. 이러한 역할은 최근에 결성된 직업성질환감시연구회와 산업안전보건연구원, 그리고 대한산업의학회, 산업간호학회, 산업위생학회 등이 협력하여 학술적인 작업과 함께 실무적인 지침을 만들어나가도록 하는 것이 바람직할 것이다.

천연항균성 물질을 이용한 Vibrio vulnificus의 살균 및 독소생성 억제효과

조성환,서일원,최종덕,전상수,라택균,정수근,강동훈 國立統營水産專門大學 附設 水産科學硏究所 1993 수산과학연구소보고 Vol.4 No.-

생선회용 해산물에 오염되어, 생식한 경우 발생되는 패혈병의 원인균주인 Vibrio vulnificus의 생육 및 독성생성을 억제할 목적으로 천연 항균제인 Grapefruit종자추출물(GPSE)을 이용하여 그 항균효과를 검토하였다. In vitro 시험결과, Vibrio vulnificus에 대한 GFSE의 생육최소저해농도는 50~100 ppm 정도이었으며, Vibrio vulnificus 균체세포틀 100 ppm 농도의 GFSE 용액으로 처리하고 전자현미경 촬영시료로 조제하여 촬영한 후, 미생물형태을 무처리대조구와 비교, 검토한 결과, GFSE 처리로 세포막 기능이 파괴되어 세포내용물이 균체외부로 유출되어 균체의 생육이 억제되고 균체내부가 빈 ghost 형태의 균체수가 증대되어 GFSE의 항균효과를 확인할 수 있었다. 한편, 생선회용 해산물에 GFSE를 근육주사하고 Vibrio vulnificus를 접종한 수조내에서 사육하여 채취한 어육부를 마쇄하여 일정량씩을 표준사료에 첨가해서 통닭용 병아리를 실험동물로 하여 사양실험을 실시한 결과. 체중증가율, 단백질이용효율, 혈청내 효소활성, 혈청의 주요성분함량 등에 있어서, GFSE 처리수조내에서 사육한 해산물을 첨가한 시료시험구의 경우, 무첨가사료처리구와 비교할 때 뚜렷한 차이를 목격할 수 없었으며, GFSE 첨가에 따른 실험동물의 성장저해 또는 독성현상을 유발하지 않음을 알 수 있었다. 아울러, 각 처리구에서 사양한 해산물의 어육부에 오염된 Vibrio vulnificus의 균수를 측정한 결과, 250ppm 이상의 GFSE 처리구에서는 Vibrio vulnificus를 전혀 검출할 수 없었다. To prevent food-poisoning outbreaks by Vibrio vulnificus the antimicrobial efficacy of grapefruit seed extract (GFSE) was examined. Minimal inhibitory concentration (in vitro) for the microorganism was found to be 50~100 ppm. Transmission electron micrographs of Vibrio vulnificus showed the biocidal action of this natural antimicrobial agent would be related to specific respiratory effect coupled with the destruction of permeable function of microbial cell membrane. After Anguilla japonia GFSE-injected to the body was incubated in the seawater contaminated by Vibrio vulnficus the fish meats were taken up, mixed with control diet and used as a diet in the feeding experiment. In this experiment the effect of GFSE treated with fish muscle on body weight protein efficiency ratio, serum enzymes and serum blood component of broiler chicks was investigated. It is proved from this study that there is neither Vibriosis nor toxicity associated with GFSE itself and fish meats treated with it when it is injected to the fish body at a level of 250 ppm or less.

안구적출에 따른 위둔덕의 칼슘결합단백질의 재구축 및 상호 연관성

안병수,고길석,안명수,김경주,권안성,정명섭,박춘매,조병옥,김진우,Samudra Acharya,Parmeshwar Narayan Amatya,장인엽 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Background: Superior colliculus is a part of midbrain, and participates in the visual reflexes, It receives afferent fibers from optic nerve, visual cortex, and spinotectal tract. After optic deprivation, the microscopic structure of the superior colliculus changed. Calcium-binding proteins (CBPs) Play an important role in the neuronal protection, differentiation and reorganization of the central nervous system, Objectives and Methods: The effects of neonatal retinal deafferentation on a CBPs, calbindm D-28k (CB), Parvalbumin (PB) and calretimn (CR), and the existence of colocalization between the CBPs were examined immunohistochemically in the rat superior colliculus. Results: On the experimental (contralateral to enucleation) side of superior colliculus, the number of CB-immunoreactive (IR) cells was reduced (77.4% compared to control), but not fibers. The number of PB-IR neurons and fibers was also reduced on the experimental side (88.5% compared to control), In the other hand, the CR-IR cells were dramatically increased (642% compared to control), but CR-IR fibers were markedly decreased on the experimental side. The colocalization between CB-CR and PV-CR was rarely observed in the superior colliculus Conclusion: These results suggest that the changes of retinotectal projection may alter the expressional pattern of CBPs in different manners; relatively stable in CB- and PV-IR neurons and plastic in CR-IR neurons.

Aerobic dance를 사용한 3개월 간의 『운동+식사 프로그램』이 비만경향 여성의 형태 및 신체조성에 미치는 영향

육조영,김범수,이승훈,전혜령 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1

The purpose of this study was to evaluate the effects of a weight-loss program in obese women. Sixty-seven subjects, aged 37.1±8.7 years, joined in the program that comprised diet and exercise prescription. The exercise prescription consisted of a 60-rnin aerobic dance(2d/wk) and self-selected, unsupervised aerobic exercise. The duration of this program was 3months. As a result, body mass and %fat significantly decreased from 61.9±8.4 to 59.1 ±8.3㎏ and from 30.7±7.5 to 26.6±7.0%(p<0.05), respectively. Fat-free mass(FFM) remained essentially unchanged (42.6±4.2 vs. 43.0±3.7㎏). About 10% of all subjects lost FFM by more than 2㎏, and accordingly, their energy yield was not enough to maintain FFM. Among the subjects who successfully reduced excess fat and maintained FFM no change in energy yield and reduction in upper body fat were found. Conversely, the subjects who could not maintain FFM lost lean tissue in lower limbs. It is suggested that our weight-loss program with exercise is useful in improving body composition and diet ingestion, and that particularly aerobic dance is considered available for reducing excess fat.

리모델링의 프로세스에 관한 연구

송천수,조형근 호남대학교 2003 호남대학교 학술논문집 Vol.24 No.2

아므르불가사리 (Asterias amurensis)의 배우자를 이용한 남해해역 연안해수의 생물학적 수질평가

유춘만,박지인,조기안,주현수,박종천,나명석 한국환경과학회 1999 한국환경과학회지 Vol.8 No.1

This study was carried out to evaluate off-shore water quality and to draw marine pollution map to Southern sea in Korea by bioassay using gametes and early development system of Asterias amurensis. From the bioassay, it was determined that the water qualites of Southern sea maintained the grade Ⅱ, because development of A. amurensis were moderately inhibited. However, Sunchon, Kwangyang, and Jinhae Bay belonged to grade Ⅲ, because development of A. amurensis were strongly inhibited. This could be due to the inflows of several river systems and poor water circulation. The water quality at Yosu island was the grade I, because A. amurensis were not inhibited in its early development.

天然洗劑의 生物分解에 관한 硏究

이춘식,최종수,박승조 동아대학교 환경문제연구소 1993 硏究報告 Vol.16 No.1

We were investigated with biodegradation of natural detergent by using jar-test, column test and activated sluge process. We were studied the BOD loading rate, the removal rate of organic material, the relationship between F/M, ratio, BOD and SS, SRT and MI, VSS/MLSS ratio. The results are summarized below. 1. In case of column height 160cm, suspended solid removal rate was 60.93%, COD, BOD and SS removal rate were 40%, 33% and 87% repectively in jar-test. In this case dosage of coagulant was 40.9g/㎥ alum, 4.5g lime/㎥ and 0.4g/㎥ polymer. 2. Wastewater containing natural detergent concentrationabout 600-1300mg/l was treated with activated sludge process and then removal rate of BOD is 80% in winter atmospheric temperature. In this case BOD concentration was about 10mg/l. 3. Wastewater containing natural detergent was treated with activated sludge process and then BOD volumetric loading rate is 0.3-0.8kg BOD/㎥·d. In this case BOD removal rate was about 90 %, MLVSS/MLSS ratio was 0.4-0.5 and SVI is 60-200.

6-Substituted Benzothiazolyl Sulfonamide 誘導體의 合成과 抗菌作用에 關한 硏究

이천수,조이섭 啓明大學校 産業技術硏究所 1988 産業技術硏究所 論文報告集 Vol.10 No.-

Ten compounds of 6-substituted benzothiazolyl sulfonamide derivatives were synthesized, and their authentic structures were identified by IR, H-NMR and ????? spectra with 2-(p-toluenesulfonamido)-benzothiazole(Ⅰ), 2-(p-toluenesulfonamido)-6-ethoxybenzothiazole(Ⅱ), 2-(p-toluenesulfonamido)-6-thiocyanatobenzothiazole(Ⅲ), 2-(p-toluenesulfonamido)-6-chlorobenzothiazole(Ⅳ), 2-(p-toluenesulfonamido)-6-methylbenzothiazole(Ⅴ), 2-(p-toluenesulfonamido)-6-ethylbenzothiazole(Ⅵ), 2-(benzenesulfonamido)-benzothiazole(Ⅶ), 2-(benzenesulfonamido)-6-ethoxybenzothiazole(Ⅷ), 2-(benzenesulfonamido)-6-nitrobenzothiazole(Ⅸ), 2-(benzenesulfonamido)-6-methylbenzothiazole(Ⅹ), Their antimicrobial activities against Staphylococcus aureus, Salmonella paratype, Escherichia coil and vibrio parahaemolyticus were investigated by the agar medium dilution method, In general, inhibitory effects of these compounds on growth of S.aureus and E. coli, and S. paratyphi and V. parahaemolyticus were showed at a concentration over 250ppm and 100ppm respectively.