청가시덩굴 추출물의 기능성 원료 표준화를 위한 지표성분 Resveratrol, trans-Scirpusin A의 분석법 개발 및 검증

권진관(Jin Gwan Kwon),정연우(Yeon Woo Jung),최윤혁(Yun-Hyeok Choi),이지은(Ji Eun Lee),정원식(Wonsik Jeong),이정아(Jung A Lee),최춘환(Chun Whan Choi),안은경(Eun-Kyung Ahn),최용문(Yongmun Choi),홍성수(Seong Su Hong) 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.11

본 연구는 HPLC를 이용하여 청가시덩굴 추출물을 개별인정형 건강기능식품의 기능성 원료로 개발하기 위한 원료 표준화의 일환으로, 청가시덩굴 추출물의 지표성분을 resveratrol과 trans-scirpusin A로 설정하고 이에 대한 HPLC 분석법을 확립하여 유효성의 검증을 실시하였다. 분석법 유효성 검증은 특이성, 직선성, 정확도, 정밀도, 검출한계 및 정량한계 등을 통해 분석법의 신뢰성을 검증하였으며, 그 결과 표준용액과 청가시덩굴 추출물 간의 HPLC 크로마토그램 및 UV spectrum의 일치 여부 등의 비교를 통해 다른 물질과 간섭 없이 피크가 분리된 것으로 특이성을 확인하였다. 또한 표준용액 검량선의 상관계수(R²)는 0.9999로 매우 우수한 직선성으로 관찰되어 분석에 적합한 것으로 확인되었으며, 검량선의 기울기 및 표준편차를 이용한 검출한계는 resveratrol이 0.98 μg/mL, trans-scirpusin A는 0.49 μg/mL였고 정량한계는 resveratrol이 2.98 μg/mL, trans-scirpusin A는 1.48 μg/mL로 각각 확인되었다. 청가시덩굴 추출물에 표준물질을 3개 농도 첨가하고 분석한 회수율은 resveratrol이 98.77~99.24%, trans-scirpusin A는 98.45~99.45%로 나타나 정확성이 있는 것을 확인할 수 있었다. 청가시덩굴 추출물의 조제 농도 2.2, 4.4 및 6.6 mg/mL에서 반복성은 resveratrol이 0.99~1.22%, trans-scirpusin A는 1.12~1.32%를, 실험실 내 정밀성에서는 일내 정밀성은 resveratrol이 0.67~0.87%, trans-scirpusin A는 1.18~1.33%로 나타났고 일간 정밀성은 resveratrol이 0.93~1.22%, trans-scirpusin A는 1.33~2.27%로 확인되어 본 분석법은 정밀성이 있음을 확인할 수 있었다. 이상의 분석결과를 통해 확립된 청가시덩굴 추출물의 지표성분인 resveratrol과 trans-scirpusin A의 HPLC 분석법은 적합한 시험법으로 검증되었으며, 본 시험법은 향후 청가시덩굴 추출물의 건강기능식품 기능성 원료 개발과 표준화를 위한 기초자료로 활용될 것으로 사료된다. This study was undertaken to establish an analytical method using high-performance liquid chromatography (HPLC). HPLC for the standard determination of resveratrol and trans-scirpusin A as functional ingredients in Smilax sieboldii extract. We evaluated the specificity, linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ) of various analytical methods for detecting resveratrol and trans-scirpusin A using HPLC. The specificity was confirmed by the chromatogram obtained using the HPLC analytical method. Also, the results of UV and the coefficient of correlation (R²) obtained was 0.999, which confirmed that this was a suitable analysis with high linearity. The LOD was 0.98, 0.49 μg/mL, and LOQ was 2.98, 1.48 μg/mL, which was confirmed as a suitable limit level for the analysis of resveratrol and trans-scirpusin A content in the S. sieboldii extract. The recovery of resveratrol and trans-scirpusin A content was determined to be 98.77±0.73∼99.24±1.47% and 98.45±1.18∼99.45±1.66%, respectively, indicating high accuracy. The intra-day repeatability and the intra-laboratory precision of the daily repetition were confirmed to be 0.67∼0.87%, 1.18∼1.33% and 0.93∼1.22%, 1.33∼2.27%, respectively, for trans-scirpusin A, for the relative standard deviation. These results indicate that the reported HPLC method is simple, reliable, and reproducible for the detection of resveratrol and trans-scirpusin A in S. sieboldii extract.

적외선열화상장치를 이용한 Buchanan plugger 표면의 온도상승 분석

최성아,김선호,황윤찬,윤창,오병주,최보영,정우남,정선와,황인남,오원만 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.4

This study was performed to evaluate the temperature rise on various position of the Buchanan plugger, the peak temperature of plugger's type and the temperature change by its touching time of heat control spring. The heat carrier system 'System B' (Model 1005. Analytic Technologies, USA) and the Buchanan's pluggers of F, FM, M and ML sizes are used for this study. The temperature was set to 200℃ which Dr. Buchanan's "continuous wave of condensation" technique recommended on digital display and the power level on it was set to 10. In order to apply heat on the Buchanan's pluggers, the heat control spring was touched for 1, 2, 3, 4 and 5 seconds respectively. The temperature rise on the surface of the pluggers were measured at 0.5 mm intervals from tip to 20 mm length of shank using the infraed thermography (Radiation Thermometer-IR temper, NEC San-ei Instruments, Ltd, Japan) and TH31-702 Data capture software program (NEC San-ei Instruments, Ltd, Japan). Data were analyzed using a one way ANOVA followed by Duncan's multiple range test and linear regression test. The results as follows. 1. The position at which temperature peaked was approximately at 0.5 mm to 1.5 mm far from the tip of Buchanan's pluggers (p<0.001). The temperature was constantly decreased toward the shank from the tip of it(p<0.001). 2. When the pluggers were heated over 5 seconds, the peak temperature by time of measurement revealed from 253.3±10.5℃ to 192.1±3.3℃ in a touch for 1 sec, from 218.6±5.0℃ to 179.5±4.2℃ in a touch for 2 sec, from 197.5±3.0℃ to 167.6±3.7℃ in a touch for 3 sec, from 183.7±2.5℃ to 159.8±3.6℃ in a touch for 4 sec and from 164.9±2.0℃ to 158.4±1.8℃ in a touch for 5 sec. A touch for 1 sec showed the highest peak temperature, followed by, in descending order, 2 sec, 3 sec, 4 sec. A touch for 5 sec showed the lowest peak temperature (p<0.001). 3. A each type of pluggers showed different peak temperatures. The peak temperature was the highest in F type and followed by, in descending order, M type, ML type. FM type revealed the lowest peak temerature (p<0.001). The results of this study indicated that pluggers are designed to concentrate heat at around its tip, its actual temperature dose not correlate well with the temperature which Buchanan's "continuous wave of condensation" technique recommended, and finally a quick touch of heat control spring for 1 sec reveals the highest temperature rise.

THE UNAM-KIAS CATALOG OF ISOLATED GALAXIES

Herná,ndez-Toledo, H. M.,Vá,zquez-Mata, J. A.,Martí,nez-Vá,zquez, L. A.,Choi, Yun-Young,Park, Changbom American Institute of Physics 2010 The Astronomical journal Vol.139 No.6

<P>A new catalog of isolated galaxies from the Sloan Digital Sky Survey Data Release 5 (SDSS DR5) is presented. A total of 1520 isolated galaxies were found in 1.4 sr of sky. The selection criteria in this UNAM-KIAS catalog are a variation on the criteria developed by Karachentseva, including full redshift information. Through an image processing pipeline that takes advantage of the high-resolution (~0<img entity='farcs' SRC='http://ej.iop.org/icons/Entities/farcs.gif' ALT='farcs' ALIGN='BOTTOM' />4 pixel<SUP>–1</SUP>) and high dynamic range of the SDSS images, a uniform g-band morphological classification for all these galaxies is presented. We identify 80% (Sa-Sm) spirals (50% later than Sbc types) on one hand, and a scarce population of early-type E (6.5%) and S0 (8%) galaxies amounting to 14.5% on the other hand. This magnitude-limited catalog is ~80% complete at 16.5, 15.6, 15.2, 14.6, and 14.4 mag in the ugriz bands, respectively. Some representative physical properties including SDSS magnitudes and color distributions, color-color diagrams, absolute magnitude-color, and concentration-color diagrams as a function of morphological type are presented. The UNAM-KIAS Morphological Atlas is also released along with this paper. For each galaxy of a type later than Sa, a mosaic is presented that includes (1) a g-band logarithmic image, (2) a g-band filtered-enhanced image where a Gaussian kernel of various sizes was applied, and (3) a red giant branch color image from the SDSS database. For E/S0/Sa galaxies, in addition to the images in (1), (2), and (3), plots of r-band surface brightness and geometric profiles (ellipticity ε, position angle PA, and A<SUB>4</SUB>/B<SUB>4</SUB> coefficients of the Fourier series expansions of deviations of a pure ellipse) are provided. The size of the sample, the redshift completeness, the availability of high-quality multicolor photometric data and detailed morphological and spectroscopic information make the UNAM-KIAS catalog of isolated galaxies a suitable sample to address important issues such as (1) comparative studies of environmental effects, (2) constraining the currently competing scenarios of galaxy formation and evolution, (3) the nature and evolution of elliptical and spiral galaxies in the field, (4) the spectral properties of a statistically significant number of isolated galaxies and their evolution as a function of redshift, and (5) the fraction of active galactic nuclei in isolated environments, among other important topics. The optimization and estimation of new structural parameters as well as important information to complement existing ones in other wavelengths is being carried out.</P>

Oxidative lipid modification of nicastrin enhances amyloidogenic γ‐secretase activity in Alzheimer’s disease

Gwon, A‐,Ryeong,Park, Jong‐,Sung,Arumugam, Thiruma V.,Kwon, Yong‐,Kook,Chan, Sic L.,Kim, Seol‐,Hee,Baik, Sang‐,Ha,Yang, Sunghee,Yun, Young‐,Kwang,Choi, Yuri,Kim, Sa Blackwell Publishing Ltd 2012 AGING CELL Vol.11 No.4

<P><B>Summary</B></P><P>The cause of elevated level of amyloid β‐peptide (Aβ42) in common late‐onset sporadic [Alzheimer’s disease (AD)] has not been established. Here, we show that the membrane lipid peroxidation product 4‐hydroxynonenal (HNE) is associated with amyloid and neurodegenerative pathologies in AD and that it enhances γ‐secretase activity and Aβ42 production in neurons. The γ‐secretase substrate receptor, nicastrin, was found to be modified by HNE in cultured neurons and in brain specimens from patients with AD, in which HNE–nicastrin levels were found to be correlated with increased γ‐secretase activity and Aβ plaque burden. Furthermore, HNE modification of nicastrin enhanced its binding to the γ‐secretase substrate, amyloid precursor protein (APP) C99. In addition, the stimulation of γ‐secretase activity and Aβ42 production by HNE were blocked by an HNE‐scavenging histidine analog in a 3xTgAD mouse model of AD. These findings suggest a specific molecular mechanism by which oxidative stress increases Aβ42 production in AD and identify HNE as a novel therapeutic target upstream of the γ‐secretase cleavage of APP.</P>

침수식 초음파 검사와 부분결합간섭 검사를 이용한 공식의 정확성 비교

윤일석(Il Suk Yun),박아람(A ram Park),최신영(Shin Young Choi),양홍석(Hong Seok Yang) 대한안과학회 2016 대한안과학회지 Vol.57 No.6

목적: 침수성 초음파 장치와 수동 각막곡률계를 이용한 인공수정체 계산공식과 IOL MasterⓇ를 이용한 계산공식의 예측오차를 비교하여 안축장과 전방 깊이, 각막곡률에 따른 침수성 초음파 검사와 수동각막곡률계를 이용한 인공수정체 검사의 정확성에 대해 보고하고자 하였다. 대상과 방법: 단일 술자에 의해 백내장수술을 받은 환자 65명 82안의 의무기록을 후향적으로 검토하였다. 생체계측은 IOL MasterⓇ, 수동각막곡률계, 침수식 방식의 초음파 검사를 순서대로 시행하여 측정하였다. 초음파 방식과 수동각막곡률계로 구한 생체계측 값을 SRK-T, Holladay 1 공식에 대입하였고 IOL MasterⓇ를 이용한 생체계측 값을 장비에 내장되어 있는 SRK-T, Holladay 2 공식에 대입하여 백내장 수술 후 굴절력을 예측하였다. 백내장 수술 후 7주 이상 지난 시점에 현성 굴절 검사를 시행하여 최종 굴절력을 구하였고, 각 공식들의 예측치와 이를 비교하였다. 결과: 평균 안축장과 평균 각막곡률값은 계측기기 간에 의미 있는 차이를 보였다. 그러나 평균절대오차는 두 계측방식 간에, 그리고 공식들 간에 안축장과 전방 깊이, 각막곡률에 따라서 의미 있는 차이가 관찰되지 않았다. 그러나 안축장과 전방 깊이, 각막곡률에 따라서 예측오차가 ±0.5디옵터 이내에 속하는 백분율은 4가지 공식들 간에 차이를 보였다. 결론: 침수식 초음파와 수동 각막곡률계를 이용한 생체계측 방식은 IOL MasterⓇ 방식과 비교하여 그 정확성이 떨어지지 않는 것으로 보인다. 그러나 더 정확한 인공수정체의 선택을 위해서는 안축장, 전방 깊이, 각막곡률에 따라서 더 적절한 인공수정체 공식을 선택하는 것이 필요할 것으로 생각된다. <대한안과학회지 2016;57(6):905-916> Purpose: To report the accuracy of intraocular lens (IOL) formulas according to axial length, anterior chamber depth, and mean corneal curvature when performing biometry with an immersion type A-scan with mannual keratomery and an IOL Master??. Methods: Retrospective medical chart reviews were carried out for 82 eyes of 65 patients who underwent cataract surgery performed by a single surgeon. Biometry was performed using IOL Master??, mannual keratometry, and immersion type A-scan ultrasound in sequence. Prediction diopter was obtained using Sanders-Retzlaff-Kraff/Theoretical (SRK-T) and Holladay 1 formulas calculated with the biometric value measured by mannual keratomery and A-scan, and using SRK-T and, Holladay 2 formulas with IOL Master??. The final refractive outcome was determined as manifested refraction at least 7 weeks after the surgery, and it was compared with the preoperative prediction dipoter (D) of the IOL formulas. Results: Mean axial length and mean keratomtric measurements as determined by A-scan with mannual keratomery showed significant statistical differences from those of IOL Master??. However, there was no difference in postoperative mean absolute error between biometric measurements, or among formulas according to axial length, anterior chamber depth, or mean corneal curvature. However, the percentage of actual refraction within ±0.50 D of the intended refraction was dirrerent among the four formalas according to axial length, anterior chamber dept, mean corneal curvature. Conclusions: Biometry measurement using the immersion-type A-scan with mannual keratomery is as accurate as that using IOL Master?? for predicting the postoperative refractive state of cataract surgery. However, it is suggested that the best IOL formula be chosen according to axial length, anterior chamber depth, and mean corneal curvature. J Korean Ophthalmol Soc 2016;57(6):905-916

Gut-Specific Delivery of T-Helper 17 Cells Reduces Obesity and Insulin Resistance in Mice

Hong, C.P.,Park, A.,Yang, B.G.,Yun, C.H.,Kwak, M.J.,Lee, G.W.,Kim, J.H.,Jang, M.S.,Lee, E.J.,Jeun, E.J.,You, G.,Kim, K.S.,Choi, Y.,Park, J.H.,Hwang, D.,Im, S.H.,Kim, J.F.,Kim, Y.K.,Seoh, J.Y.,Surh, C. Elsevier North Holland [etc.] 2017 Gastroenterology Vol.152 No.8

<P>BACKGROUND & AIMS: Obesity and metabolic syndrome have been associated with alterations to the intestinal microbiota. However, few studies examined the effects of obesity on the intestinal immune system. We investigated changes in subsets of intestinal CD4(+) T-helper (T-H) cells with obesity and the effects of gut-tropic T(H)17 cells in mice on a high-fat diet (HFD). METHODS: We isolated immune cells from small intestine and adipose tissue of C57BL/6 mice fed a normal chow diet or a HFD for 10 weeks and analyzed the cells by flow cytometry. Mice fed a vitamin A-deficient HFD were compared with mice fed a vitamin A-sufficient HFD. Obese RAG1-deficient mice were given injections of only regulatory T cells or a combination of regulatory T cells and T(H)17 cells (wild type or deficient in integrin beta 7 subunit or interleukin 17 [IL17]). Mice were examined for weight gain, fat mass, fatty liver, glucose tolerance, and insulin resistance. Fecal samples were collected before and after T cell transfer and analyzed for microbiota composition by metagenomic DNA sequencing and quantitative polymerase chain reaction. RESULTS: Mice placed on a HFD became obese, which affected the distribution of small intestinal CD4(+) T-H cells. Intestinal tissues from obese mice had significant reductions in the proportion of T(H)17 cells but increased proportion of T(H)1 cells, compared with intestinal tissues from nonobese mice. Depletion of vitamin A in obese mice further reduced the proportion of T(H)17 cells in small intestine; this reduction correlated with more weight gain and worsening of glucose intolerance and insulin resistance. Adoptive transfer of in vitro-differentiated gut-tropic T(H)17 cells to obese mice reduced these metabolic defects, which required the integrin beta 7 subunit and IL17. Delivery of T(H)17 cells to intestines of mice led to expansion of commensal microbes associated with leanness. CONCLUSIONS: In mice, intestinal T(H)17 cells contribute to development of a microbiota that maintains metabolic homeostasis, via IL17. Gut-homing T(H)17 cells might be used to reduce metabolic disorders in obese individuals.</P>

Eicosanoid 유도체가 흰쥐 혈압 변화에 미치는 영향

윤재순,윤연숙,신정희,최현진,최진아 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

Arachidonic acid (AA, C20:4, w-6) and eicosapentanoic acid (EPA,C20:5, w-3), which are polyunsaturated fatty acids forming eicosanoids, were tested for their effects on blood pressure in Wistar rats and SHR. AA is the most important precursor for the biosynthesis of eicosanoids which include the prostaglandins, prostacyclin (PGI_2), thromboxane A_2 (TXA_2) and the leukotriens. TXA_2 is a potent vasoconstrictor and a powerful inducer of platelet aggregation causing myocardial infarction and hypertention. In contrast, PGI_2 induces vasodilation and inhibits platelet aggregation. In this study, AA markedly increased blood pressure, but its effect was antagonized by both EPA a structural analog of AA, and dazmegrel, a TX synthetase inhibitor. Also, AA enhanced the antihypertensive effects of hydralazine and captopril, and EPA reduced TXA_2 production. These results indicate that the hypotensive effects of EPA might be closely related to the decrease in TXA_2 biosynthesis due to competitive inhibition by structural similarity of the EPA to the AA, the precursor of TXA_2.

Addition of agricultural byproducts to feeding substrate affects growth performance and nutritional value of mealworms, Tenebrio molitor (Coleoptera: Tenebrionidae)

Sang A Oh,Duck-Soo Choi,Do Ik Kim,Sun Am Kim,Ji Soo Kim,Yun Ji Lim,Ju Young Lee 한국응용곤충학회 2024 한국응용곤충학회 학술대회논문집 Vol.2024 No.04

갈색거저리 유충의 사료인 밀기울은 대부분 수입에 의존하고 있는데 일부 국가의 식량 수출 중단 조치 등에 따른 국제 곡물가격 상승으로 밀기울 가격은 인상되고, 식용곤충 판매가격이 하락하면서 생산비 절감을 위한 사료 개발이 요구되고 있다. 농업부산물 3종을 50% 이상 급이하게 되면 유충 생육이 저하되었기 때문에 본 연구는 적정한 배합비율을 선정하기 위해 첨가사료 20, 30% 함량으로 밀기울과 혼합하여 사료를 급이하였을 때 갈색거 저리 유충의 생육 특성과 영양성분 변화에 대해 밀기울만 제공한 대조구와 비교하였다. 부산물 A와 B, C를 각각 30% 함유한 처리구에서 갈색거저리 유충 무게는 대조구와 차이가 없음을 확인하였다. 먹이소화율은 부산물B를 20% 함유한 처리구가 80.5%로 대조구에 비해 높았고, 부산물C 30% 처리구에서 72.6%로 가장 낮았다. 갈색거저 리 유충의 생육일수 100일 기준으로 부산물B 30% 처리구에서 용화율이 76.1%로 대조구보다 1.6배 높았으며, 부산물A 20% 처리구는 29.2%로 용화율이 가장 낮았다. 갈색거저리 유충의 조단백질 함량은 부산물 C 30% 처리 구에서 대조구보다 10.3% 증가하여 아미노산 분석을 진행한 결과 sarcosine과 ornithine이 2.5배 이상 증가하였다. 이를 통해 부산물 B나 C를 30% 함유한 사료를 급이하였을 때 사육 원가를 절감하여 유충을 생산할 수 있을 것으로 사료된다.

Al2(SO4)3, AlK(SO4), MgSO4 및 CuSO4의 희석액 열탕처리가 압화용 황색 카네이션 꽃잎의 변색에 미치는 영향

박윤점 ( Yun Jum Park ),안정숙 ( Hung Suk Ah ),김현아 ( Hyun A Kim ),김현주 ( Hyun Ju Kim ),최경혜 ( Koung Hea Choi ),김유진 ( Yu Jin Kim ),허북구 ( Buk Gu Heo ) 한국화예디자인학회 2008 한국화예디자인학 연구 Vol.19 No.-

This study was conducted to develop the preservation technique of materials for the pressed flower from discoloration. We have collected yellow carnation petals, and immersed that in Al2(SO4)3, AlK(SO4), MgSO4 and CuSO4 solution diluted with hot distilled water (85-90℃) by 0, 5, 10, 20 and 30% for 30 seconds. We dried and applied petals ultraviolet rays for 21 days and examined into those discoloring degree. Hunter L*, a*, b* and △E* values for that immersed in Al2(SO4)3 and MgSO4 solution diluted by 30% showed the least discoloring degree. Discoloration of carnation petals in terms of Hunter L*, a* and b* values immersed in 30% diluted AlK(SO4 ) solution, and △E* values in 5% solution had the least changes compared with that in control. Those hunter L*, a*, b* and E* values treated with 20% CuSO4 diluted solution had the smallest changes. Consequently, it was assumed that immersing the yellow carnation flowers in diluted Al2(SO4)3, AlK(SO4), MgSO4 and CuSO4 solution was a appropriate way to preserve the petals from discoloration for the pressed flower.

축하화환에서 폐기되는 적색 거베라 추출액을 이용한 화훼디자인용 망사잎의 염색성

박윤점 ( Yun Jum Park ),안정숙 ( Hung Suk Ah ),김현아 ( Hyun A Kim ),김현주 ( Hyun Ju Kim ),최경혜 ( Koung Hea Choi ),허북구 ( Buk Gu Heo ) 한국화예디자인학회 2008 한국화예디자인학 연구 Vol.19 No.-

This study investigated special quality of Gerbera extract and stainability of skeletonized leaves along with examination for wasted at celebration wreath gerbera(Gerbera hybrida Hort.) use as nature dye. Dyed by gerbera extracts 1.50%, as the dyeing times increase 10 to 60 minutes, color differences were significant, and color value showed contrary tendency. a* value were 5.21 to 8.39 in case of skeletonized leaves. Effect of dyeing temperature, a* value showed the maximum value 9.07 of skeletonized leaves dyed at 90℃. They showed sudden increase at the extract density of 1.50% and showed slow increase at more than 1.50% density. a* value showed the maximum value 10.05 of skeletonized leaves dyed at 2.00% liquid. Stainability that follow for pH of extraction liquid is pH 1 to 8.29 and red color decreased a little in pH 3~7, and silk fabric in pH 7 move that to green direction in pH 5 appear. Color difference and color fastness according to the kinds of mordants and timing of mordants were getting significantly increased at the processing of mordants on the whole. Skeletonized leaves dyed by iron extract were significantly increased in case of the kinds of mordants, skeletonized leaves were bigger at the pre mordants. When treat mordanting after dyeing in gerbera extraction liquid by sequence of above, I could know various tone and red color, red-yellow, yellow etc. of become color development variously and value as nature dye.