일산화탄소가 뇌내 신경전달물질 및 그 합성효소에 미치는 영향

윤재순 梨花女子大學校 藥學硏究所 1991 藥學硏究論文集 Vol.- No.1

We studied the effect of carbon monoxide(CO)-induced bypoxia on monoamine neurotransmitter and their synthetic enzyme in rat brain. When the rats were acute or chronic intoxicated at CO 5000 ppm for 30 minutes or 2000ppm for 1 week each 3hours, dopamine content increased significantly with decreasing of its turnover on striatum and norepinephrine content was diminished in hypothalamus. 5-hydroxytryptamine content was increased significantly and its turnover was decreased both in striatum and hypothalamus. Tryosine hydroxylase activity was reduced in striatum. These results suggest that inhibition of TH activity in CO-induced hypoxia is owing to lack of oxygen supply threfore NE content is decreased. We suggest that increasing of dopamine and 5-hydroxytryptamine are due to reduction of its turnover.

6-Hydroxydopamine이 Clonidine의 抗高血壓 效能에 미치는 影響

尹再順,李恩珠 梨花女子大學校 韓國生活科學硏究院 1986 韓國生活科學硏究院 論叢 Vol.38 No.-

6-Hydroxydopamine(6-OHDA)은 交感神經切除 效果가 있다는 보고가 있어 中樞性抗高血壓藥 clonidine과 삼환계항우울약 desipramine 병용시의 효능변화와 DOCA-salt 처치로 高血壓症發生에 미치는 交感神經의 역활을 검토하고저 6-OHDA을 출생시 투여하여 抗高血壓藥物??효과에 미치는 영향을 관찰하였다. 정상쥐나 DOCA-salt 유발고혈압쥐에서 유의적인 큰 폭으로 나타난 clonidine의 抗高血壓反應은 6-OHDA 처치로 거의 나타나지 않았다. Desipramine 전처치로도 매우 유의적으로 억제되었다. 6-OHDA을 전처치한 쥐는 desipramine 투여에 관계없이 clonidine의 抗高血壓反應이 억제되었다. 또한 6-OHDA 처치한 후 DOCA-salt 투여시의 抗高血壓症도 미약하였다. 이상의 결과에서 6-OHDA, clonidine 및 desipramine은 모두 뇌신경과의 높은 친화력으로 中樞性 抗高血壓症發現이나 억제에 中樞交感神經을 개재하여 큰 영향을 미치며 특히 6-OHDA은 여러 요인의 抗高血壓發生初期의 기전에 중요한 역할을 하는 것으로 나타났다. The effect of neurotoxic compound 6-hydroxydopamine (6-OHDA) on the change in blood pressure by centrally acting agents has been studied in normal and deoxycorticosterone acetate-salt induced hypertensive rats. Administration of clonidine (100㎍ kg^-1 i.v.) lowered the blood pressure in rats. In the case of neonatally 6-OHDA (2×l00mg, 250mg kg^-1 s.c.) treated rats, the antihypertensive effect of clonidine was not shown. And there is significant difference (p<0.01) to be compared with the effect of clonidine alone in normal rats. The hypotensive response of clonidine was reduced markedly by simultaneously administered desipramine (600,㎍ kg^-1 i.v.) in normal and DOCA-salt induced hypertensive rats, but reduced without regard to treatment and untreatment of desipramine in neonatally 6-OHDA treated sympathectomized rats. Administration of 6-OHDA prevented the development of DOCA-salt hypertensive rats. In this hypertensive rats, the hypotensive response of clonidine was significantly reduced by 6-OHDA or desipramine pretreatment. These results suggest that high affinity with central neuron in brain of 6-OHDA, clonidine and desipramine was high significant correlation in development and inhibition of central hypertension mediated central adrenergic neuron.

Piracetam이 마우스의 기억력 및 대뇌피질내 Acetylcholine량 변화에 미치는 영향

尹再順,申精姬,李容筍,劉永瓚 梨花女子大學校 韓國生活科學硏究院 1991 韓國生活科學硏究院 論叢 Vol.47 No.-

The central cholinergic synapse is known to be closely connected with memory. Previously, piracetam has been reported to improve the intellectual ability. We used a scopolamine-induced amnesiac mouse to study the effects of piracetam on memory retrieval by passive avoidance learning test and on the acetylcholine level of cerebral cortex. The amnesia was produced in mouse by the intraperitoneal injection(i.p.) of scopolamine at a dose of 1 ㎎/㎏ body weight(b.w.). Upon administration of piracetam to amnesiac mouse at doses of 100 and 200 ㎎/㎏ b.w., i.p., 30 min, prior to retrieval test, the significant improvement on memory impairment was observed by examination 24 hr after training. The level of acetylcholine in cerebral cortex of mouse was decreased by the scopolamine treatment, however, this decrease was attenuated by piracetam. These results suggest that the effect of piracetam on memory may be attributed to activation of cholinergic nerve.

內分泌先調疾患에 대한 高麗人蔘의 藥效에 대한 硏究 : 甲狀腺疾患에 대하여 In Hypo or Hyperthyroidism

尹再順 梨花女子大學校 韓國生活科學硏究院 1980 韓國生活科學硏究院 論叢 Vol.26 No.-

호르몬으로 인한 甲狀腺機能亢進症과 低下症의 血淸中 酸素活性, 脂質, 蛋白質, 糖量 등을 측정하였을 때 亢進症에서는 S-GOT, S-GPT의 活性度 上昇, 總 cholesterol, trigyceride, 總脂質量 低下, 蛋白質量 低下, 血糖量 上昇과 같은 病的 狀態가 나타났으며, 여기에 人參을 투여하면, S-GOT, S-GPT의 活性度 上昇은 억제되고 血糖量은 低下되는 藥效를 나타내었고, 其他에 대해서는 통계학적으로 有意性 있는 영향을 미치지 못하였다. 低下症에서는 S-GOT, S-GPT, S-LDH 活性上昇, 總 cholesterol. 總脂質量 上昇, trigly-ceride 量 低下, 蛋白質量 上昇, 血糖量 低下와 같은 病的 狀態를 역시 招來했으나 人參 투텨로 酵素活性은 低下되고 總 cholesterol, 總脂質量의 증가는 억제되었고, 血糖量도 증가되어 正商化하는 데 有效하였다. 즉 TH 內分泌失調症患인 亢進症과 低下症의 血淸 중 成分變動은 人參으로 正商化되는 데 有效하였으며, 有害作用은 없었다. Activities of S-GOT, S-GPT, and S-LDH in rat serum those have been in hypo and huper-thyridism were investigated under the influences of ginseng extract in order ot find the thera-perutic effect. The levels of total lipids, proteins and glucose were measured concerning with the same aims. Activites od S-GOT, S-GPT, and levels of the serum glucose were proved to increase in hyperthyroidism, however levels of total cholesterol, triglyceride and total lipids were reduced. Raising of S-GOT, S-GPT activities and glucose level were stabilized to normal level by treatment of ginseng extract. In hypothyroidsm, activities of S-GOT, S-GPT, and S-LDH were found to increase, levels of total cholesterol, total lipies and proteins in serum increased altogether. But reductuon was observed in the levels of triglyceride and glucose in the same serum. Adding the ginseng extract, acthivities of the above three enzymes were stabilized, and cholesterol and lipids were normalized in their levels. Variation of these levels caused by either hypo or hyperthyroidism were found to be stabi-lizabie by the addition of ginseng extract giving no side effects.

Aloe vera가 Alloxan 糖尿흰쥐의 血糖量 및 Insulin量에 미치는 영향에 관한 硏究

尹再順,金惠善 梨花女子大學校 韓國生活科學硏究院 1983 韓國生活科學硏究院 論叢 Vol.31 No.-

Aloe vera Linne가 組織再生作用과 細胞復活作用이 있으며 췌장 β-cell의 insulin分泌上皮細胞의 增植過程을 촉진한다는 報告가 있어 A. vera의 糖尿病에 對한 效果와 그 作用機轉을 규명하고 저 alloxan 糖尿흰쥐와 epinephrine에 의한 一時的 過血糖흰쥐에게 A. vera, chloropropamide 또 두 藥物을 倂用投與하여 血糖量과 glycogen量 및 血中 insulin量의 變化를 Somogyi-Nelson法과 Radioimmunoassay法으로 各各 測定하였다. A. vera는 alloxan糖尿흰쥐에 있어서 血糖量降下作用과 肝藏中 glycogen含量增加作用이 있었으며 epinephrine投與에 의한 一時的過血糖흰쥐에 對해서 A. vera前處置로 血糖量上昇作用이 抑制되었다. 또한 alloxan 糖尿흰쥐에 對해서 A. vera는 insulin分泌促進作用으로 血中 insulin量이 增加되었다. They reported that Aloe vera was effective in regeneration of wound tissues and cells, and that Aloe vera stimulated the proliferative process in regeneration of insular epithelium of the pancreas. This experiment was carried out to investigate the effect of Aole vera on Diabetes mellitus. Crud powder of Aloe vera and chloropropamide were given orally to alloxan diabetic rats either alone or in combination. Level of blood glucose and serum insulin in alloxan diabeic rats were measured by Somogyi-Nelson method and radioimmunoassay method respectively. We also investigated that effect of Aloe vera on blood glucose level in transient hyperglycemic rats by epinephrine. Thus, it is possible to conclude that Aloe vera had lowering effect on blood glucose level and increasing effect on glycogen content in alloxan diabetic rats, inhibiting effect on induction of transient hyperglycemia by epinephrine, and stimulaing effect on the recretion of insulin in alloxan diabetic rats.

Eicosanoid 유도체가 흰쥐 혈압 변화에 미치는 영향

윤재순,윤연숙,신정희,최현진,최진아 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

Arachidonic acid (AA, C20:4, w-6) and eicosapentanoic acid (EPA,C20:5, w-3), which are polyunsaturated fatty acids forming eicosanoids, were tested for their effects on blood pressure in Wistar rats and SHR. AA is the most important precursor for the biosynthesis of eicosanoids which include the prostaglandins, prostacyclin (PGI_2), thromboxane A_2 (TXA_2) and the leukotriens. TXA_2 is a potent vasoconstrictor and a powerful inducer of platelet aggregation causing myocardial infarction and hypertention. In contrast, PGI_2 induces vasodilation and inhibits platelet aggregation. In this study, AA markedly increased blood pressure, but its effect was antagonized by both EPA a structural analog of AA, and dazmegrel, a TX synthetase inhibitor. Also, AA enhanced the antihypertensive effects of hydralazine and captopril, and EPA reduced TXA_2 production. These results indicate that the hypotensive effects of EPA might be closely related to the decrease in TXA_2 biosynthesis due to competitive inhibition by structural similarity of the EPA to the AA, the precursor of TXA_2.

Dactylomyces thermophillus의 代謝産物에 關한硏究

尹再順 梨花女子大學校 韓國生活科學硏究院 1975 韓國生活科學硏究院 論叢 Vol.15 No.-

Terremutin, terreic acid, and terrein were obtained from Dactylomyces thermophillus. When D. thermophillus was grown on a medium consisting of soybean meal, dextrose, yeast extract, K_2HPO_4 and NaCl under well shaken condition, a large quantity of terremutin was accumulated at an earlier stage of growth, and the compound was transformed to terreic acid at a later stage. On the other hand, terremutin and terrein were accumulated under relatively less shaken condition. When the microbe was grown on another medium consisting of peptone, dextrose, yeast extract and KH_2PO_4, terrein was accumulated. The microbe was also grown well on a mineral dextrose media consisting of (NH_4)_2SO_4, MgSO_4, K_2HPO_4, KH_2PO_4, CaCO_3, dextrose and yeast extract. However, none of those compounds were accumulated. On the basis of U.V absorption spectral analysis for metabolites accumulated in culture broth, a revised biosynthetic pathway of terreic acid was postulated.

일산화탄소 중독 저산소증 마우스의 대뇌피질 신경세포 분포에 관한 연구

尹再順,安有淑,李熙來 梨花女子大學校 韓國生活科學硏究院 1991 韓國生活科學硏究院 論叢 Vol.47 No.-

We studied the changes of the distribution on nerve cells in cerebral cortex of hypoxic mice induced by carbon monoxide(CO). When the mice exposed to 5,000 ppm CO for 60 minutes, carboxyhemoglobin level was 82%. At zero, 1st, 3rd, 5th, 7th and 14th day after CO intoxication, hypoxic and normal mice were fixed by 3.5% formalin and each of motor(area 4), somatosensory(area 3) and visual(area 17) cortices of the cerebral cortex, was sectioned and stained with cresyl violet. Using light microscope quantitative analysis was followed. Our results could be summarized as follows : The insignificant and significant decreases in number of nerve cells, were observed in the motor and somatosensory cortices, at zero, 1st day and at 3rd, 5th, 7th, 14th day after CO intoxication respectively. The same mode of decreases in the number of nerve cells was also observed in the visual cortex, at zero and at 1st, 3rd, 5th, 7th, 14th day after CO intoxication respectively. A series of decreasing phenomena in the above results were more evident in visual cortex than motor and somatosensory cortices. It may be concluded from these findings that the decrease of nerve cells in the mice exposed to CO causes the damage of nerve cell in the cerebral cortex and it is connected with necrosis.

Eicosapentaenoic acid의 藥理效果에 關한 硏究

尹再順 梨花女子大學校 韓國生活科學硏究院 1984 韓國生活科學硏究院 論叢 Vol.34 No.-

Trienoic prostaglandins의 전구체 Eicosapentaenoic acid(EPA)를 다량 식이로 섭취하면 急性心筋梗症, 動脈硬化를 방지하고 오히려 출혈경향이 있다. 따라서 EPA가 혈압에 미치는 영향을 규명해보고자 EPA 투여후 마취 흰쥐의 혈압을 측정하였다. EPA 200㎎/㎏ 투여시 23∼31%의 혈압 강하작용이 2시간 지속되었고 indomethacin(Ind.) 2㎎/㎏ 5일간 전처리로 EPA에 의한 혈압강하작용이 39.0∼74.0% 억제되었고 H-blocker 전처리로는 69∼89%까지 억제되었다. 또 적출혈관에 대한 수축력을 측정하였다. 흰쥐 흉부 대동맥은 EPA에 의해서 norepinephrine(NE) 의존수축반응곡선을 약간 완화하였고 토끼의 적출상장간막동맥은 EPA 10^-3M 용액에서 NE 의존수축반응곡선을 현저히 억제하였으며 최대수축력이 control의 25%에 불과했다. 또한 Ind. 3X10^-6M 전처리로 EPA 10^-3M 용액에서 NE 3X10^-4M 적용시의 수축반응이 58.3%가 억제되었다. 결론적으로 EPA는 혈압강하작용이 있으며, 이것은 Ind.과 H-blocker로 억제되었고 혈관에 대한 수축작용은 동물종, 혈관부위에 따라 다르다. Epidemiologic studies suggested that dietary intake of eicosapentaenoic acid(EPA), a precursor of trienoic prostaglandins is associated with a low incidence and reduced extent of myocardial infarction and atherosclerosis. The purpose of this study was to investigate the pharmacological effect of EPA on blood pressure and isolated vascular reactivity. EPA was infused directly into femoral vessel of anesthetized rats and carotid arterial blood pressure was measured. Blood pressure of anesthetized rats were observed to be lowered by 23.0∼31.0% and continued for 2 hours by administration of EPA 200㎎/㎏. The hypotensive action induced with EPA was inhibited by 39.0∼74.0% by pretreatment with indomethacin 2㎎/㎏ for 5days. The contractile force to isolated thoracic aorta and superior mesenteric artery from rats and rabbits were measured. When EPA was infused into organ bath with isolated vascular duct, the spiral strips of rat and rabbit thoracic aorta were weakly inhibited and superior mesenteric arterial strips isolated from rabbits were significantly inhibited in response to the contractile effect of norepinephrine (NE). From the above results we conclueded that EPA showed the significant hypotensive response depending on the administerd dosage and inhibited in dose-response to the contractile effect of NE according to animal species and parts of vascular system.

Phytic Acid의 抗酸化 效果에 關한 硏究

尹再順 梨花女子大學校 韓國生活科學硏究院 1976 韓國生活科學硏究院 論叢 Vol.16 No.-

A new antioxidant, phytic acid on epinephrine and liver oil composed of V-A and V-D was studied. As epinephrine, adrenal medullary hormone was contacted with air and light, it was oxidized in red colour and lost biologucal activities. Moreover, as epinephrine existed in water or contained alkali and trace metal from crude glass, its oxidation was peomoted so much the more. So, after 0.2, 0.15, 0.1, 0.08, 0.05, 0.01% phytic acid and oxidizing agent were added in epinephrine solutions, oxidative ration to adrenochrome from epinephrine was determined. Oxidative ratio of epinephrine was 95.7% in control, 1.4% in epinephrine solution added NaHSO_3 and 1.2% in epinephrine solution added 0.2% phytic acid. It is certain, therefore, that phytic acid, antioxidizing agent was most effective for the sake of preventing the oxidation of epinephrine. The antioxidative effect of epinephrine solution polluted with trace metal was also more effectivive in 0.2% phytic acid than in control or 0.08 w/v% NaHSO_3 solution. In spite of liver oil containing V-A and V-D was stored in lightlessness, its biological effect was decreased in 10-20% for one year. It is an oxidizing phenomenon of liver oil. The more oil is oxidative, the more peroxide values are increased. Therefoer, after 0.5, 0.1, 0.05, 0.01, 0.005% phytic acid were added in oil, the antioxidizing effects of materials were added in oil, the antioxidizing effects of materials were measured by the peroxide development in the oil after 5 hours under P.O.V. condition. The P.O.V. in conteol oil was 26, but in contrast with the control, P.O.V. in oil added 0.5% phytic scid was 7. Thus, it can be concluses that phytic acid is very effective for the prevention of oxidizing of epinephrine and liver oil composed of V-A and V-D.