NaOCl의 적용 후 치수강 상아질에 대한 결합강도의 변화와 Sodium Ascorbate에 의한 환원 효과

권수미,김태균,유미경,이광원 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.6

본 연구에서는 치수강이 개방된 근관와동에 5.25% NaOCl용액을 각각 다른 시간 동안 적용시킨 뒤 접착레진에 의한 미세인장결합강도의 변화를 측정하였으며, 10% sodium ascorbate를 NaOCl 용액에 일정시간 노출시킨 상아질에 다시 10분 동안 적용하여 결합강도에 미치는 효과를 연구하고자 하였다. 연구결과 본 실험에서 상부 치수강 상아질과 하부 치수강 상아질에 대한 결합강도는 모든 실험군내에서 유의한 차이 (p > 0.05)를 보이지 않았다. 5.25% NaOCl 용액을 적용하지 않고 치수강 상아질에 접착시킨 대조군에 비해 20분간 적용시킨 실험군에서는 결합강도의 감소가 초래되었으나 통계적인 유의성 (p > 0.05)은 없었다. 그러나 5.25% NaOCl 용액을 40분과 80분 동안 치수강 상아질에 적용시킨 실험군에서는 대조군에 비해 유의하게 낮은(p < 0.05) 결합강도의 변화를 나타내었다. 5.25% NaOCl 용액을 40분동안 그리고 10% sodium ascorbate를 10분간 처리한 실험군에서는 유의한 결합강도의 회복을 나타내었다. 그러나 5.25% NaOCl 용액을 80분 동안 그리고 10% sodium ascorbate를 10분간 적용한 실험군에서는 대조군에 비해 통계적으로 유의한 차이(p < 0.05)를 보임으로써 여전히 결합강도가 회복되지 못하고 있음을 나타내었다. 5.25% NaOCl 용액을 5분간 적용한 뒤 10% sodium ascorbate를 10분간 적용한 실험군에서는 오히려 대조군에 비해 높은 결합강도(p < 0.05)를 보여줌으로써 높은 회복효과를 나타내었다. Clinical suggestion for the limitation of application time of NaOCl solution is needed to avoid large reductions in resin-dentin bond strength. The aim of this study was to measure the change of -tensile bond strength after the various application time of 5.25% NaOCl solution to pulp chamber dentin in endodontic access cavity, and to evaluate the effect of 10% sodium ascorbate application for 10 min on bond strength after the treatment of 5.25% NaOCl solution. In this experiment, there were no statistical differences(p > 0.05) in bond strengths between upper chamber dentin and lower chamber dentin. NaOCl-treated group for 20 min did not show any significant decrease(p > 0.05) in bond strength than non-treated control group. In contrast to that, bond strengths of NaOCl-treated groups for 40 & 80 min were significantly lower(p < 0.05) than that of non-treated control group. 10% sodium ascorbate retreated group for 10 min after 5.25% NaOCl application for 40 min to chamber dentin showed the recovery of bond strength significantly. However, the bond strength of sodium ascorbate retreated group after 5.25% NaOCl application for 80 min was still significantly lower(p < 0.05) compared to the non-treated control group, which means the reductions in resin-dentin bond strength were not fully reversed. On the contrary, sodium ascorbate retreated group after 5.25% NaOCl application for 5 min showed significantly higher(p < 0.05) bond strength compared to the control group, which demonstrates its superior recovery effect. In SEM exminations of specimens retreated with 10% sodium ascorbate after NaOCl application for 40 & 80 min showed that resin tags were formed clearly and densely, but weakly in density and homogeneity of individual resin tag compared to the control specimen.

파종시기가 다른 일미찰의 등숙 중 호화 특성 비교

김미정, 박효진, 이유영, 김선림, 김정태, 우관식, 권영업, 정일민 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

This study was conducted to investigate the pasting properties of waxy corn ‘Ilmichal’ during ripening with different sowing date. Starch content of Ilmichal was increased as matured (p<0.05). Analysis of pasting properties with waxy corn starch using a rapid visco analyser (RVA) showed linear changes in peak viscosity, trough viscosity, final viscosity, breakdown and consistency according to ripening. There were significantly positive correlation between starch content of Ilmichal and peak viscosity (0.39*), trough viscosity (0.58***), final viscosity (0.58***), and consistency (0.57**). Differential scanning calorimerty (DSC). The enthalpy of gelatinization was increased as ripening. But onset and peak temperature were decreased. There were significantly positive correlation between starch content of Ilmichal and onset temperature (0.44*), peak temperature (0.38*), and completion temperature (0.59***), on the other hand, crude protein content presented significantly negative correlation between completion temperature (-0.41*).

신규간호사를 위한 맞춤형 멘토 프로그램(새싹프로그램)의 개발 및 적용 : 일개 대학병원 중환자실을 중심으로

권은옥,조정숙,송경자,최스미,장선주,김주희,박승현,신효연,유미,김정아 대한간호학회 간호행정학회 2009 간호행정학회지 Vol.15 No.4

Purpose: The purpose of this study was to develop specialized mentor program to improve adaptation for new nurses of intensive care unit and to identify the effect of the program on early resignation rate. Methods: The study adopted nonequivalent control group, non synchronized design. Based on the identification of problems and the needs of new nurses, a 6 month specialized mentor program was developed. The program was consisted of three parts; developing knowledge, improving interpersonal relationship and increasing coping competency for emergency situation. Data were collected between July 2006 and 2008 from 37 nurses and the early resignation rate was compared before and after the implementation of the mentoring program. Results: Early resignation rate of the nurses who received the mentor program was significantly lower than that of the nurses who didn't. The resignation rate within 1 year dropped from 44.5% to 8.3%(p=.034). Conclusions: The specialized six month mentoring program was effective in reducing early resignation rate.

대량의 각혈로 사망한 장티푸스 1예

한상우,유진홍,권순석,김성훈,박지찬,홍은정,최경성,박용완,장준희,안지원,박유경,강지영,박상미,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

We experienced a 25 year-old male patient with typhoid fever complicated with massive hemoptysis. Pulmonary complication in typhoid fever is very rare and to our knowledge, there has been no report of hemoptysis as a main cause of death with this disease. We herein report a rare case of typhoid fever.

국내 설사환자로부터 분리된 Escherichia coli 균의 혈청군 및 Verocytotoxin 생성능

김호훈,강연호,김성한,박미선,유재연,이복권 대한감염학회 1998 감염 Vol.30 No.5

목적: 국내 설사 환자에게서 분리 동정된 인체 감염 유래 E. coli 균의 혈청군, verocytotoxin 생성능을 구명하여 EHEC 균의 인체 감염 실태를 구명하고, 동물 및 식품 유래 분리균주의 성상과 비교 검토하여 인체 감염 위험성에 대한 기초적 자료를 의학계에 제공하고자 하였다. 방법: EHEC 감염의심 설사 환자 분변 검체로부터 분리된 균주 중 생화확적 성상에 따라 대장균을 분리하였고 이들 균주 중 E. coli O157:H7를 분리하기 위해서는 Dsorbitol 음성균을 선별하였으며, 분리균에 대하여 항혈청으로 응집 시험을 실시하여 혈청군을 구명하였다. 혈청군이 확인된 균주에 대하여 중합효소 연쇄반응 (PCR) 및 역수동라텍스 응집시험 (Reversed Passive Latex Agglutination:RPLA) 으로 verocytotoxin 생성능을 구명하였다. 결과: 장출혈성 대장균 감염 의심설사 환자의 분변검체로부터 25주의 E. coli 균을 분리 동정하였으나 이들 균주는 모두 verocytotoxin을 생성하지 않았다. 분리 동정된 대장균으로부터 확인된 혈청군은 16종으로서 E. coli O1, O6, O8, O15, O20, O25 O26, O28, O29, O44, O86a, O119, O126, O128, O152, 및 O157:H-였으며 E. coli O157:H-혈청형 균주와 E.coli O25 혈청군 균주가 각각 3주씩 분리되어 비교적 높은 분리율을 보였다. 결론: 장출혈성 대장균 감염 의심설사 환자 분변 가검물로부터 총 25주의 E. coli균을 분리 동정하였으나 verocytotoxin을 모두 생산하지 않았다. Background: Since 1982, many countries has reported outbreaks or sporadic cases caused by enterohaemorrhagic Escherichia coli (EHEC) serogroup strains, mainly E. coli O157:H7 type strain. However, systemic investigation about EHEC agents, including E. coli O157:H7, have not been done in Korea. Therefore, we investigated serogroup and verotoxin productivity of E. coli strains isolated from diarrheal patients and estimated risk of human infection in comparison with EHEC strains isolated from cow, pig, and food material in Korea. Methods: Diarrheal patient stool samples were collected and E. coli strains were isolated, according to biochemical characteristics. In order to isolate E. coli O157:H7, D-Sorbitol negative strains were selected. Serogrouping of the E. coli isolates was done by agglutination test. Verocytotoxin productivity was investigated by polymerase chain reaction (PCR) and reversed passive latex agglutination (RPLA). Human infection risk was estimated in comparison with EHEC strains isolated from cow, pig and food materials in Korea. Results: Twenty-five E. coli strains were isolated from the diarrheal patients who were suspected to be infected with EHEC. However, none of these E. coli strains produced verocytotoxin. Out of 25 E. coli isolates, 16 serogroups of E. coli O1, O6, O8, O15, O20, O25, O26, O28, O29, O44, O86a, O119, O126, O128, O152 and 157:H-were found. In each of the E. coli O157:H-and O25 serogroups 3 strains were found. Conclusion: None of 25 E. coli isolated from diarrheal patients who were suspected of EHEC infection produced verocytotoxin producing E. coli have been reported recently in Korea.

연구논문 : 과학영재교육원 학생들의 과학 통합 탐구 능력

정은영 ( Eun Young Jeong ),권이영 ( Yi Young Kwon ),양주성 ( Joo Sung Yang ),고유미 ( Yu Mi Ko ) 경북대학교 과학교육연구소 2013 科學敎育硏究誌 Vol.37 No.3

과학영재교육원 학생들이 성공적으로 과학 탐구를 수행하기 위해서는 문제 인식, 가설 설정, 탐구 설계, 결론 도출 등의 과학 통합 탐구 능력이 요구된다. 이 연구에서는 김유향(2013)이 개발한 ‘과학 탐구 사고력 측정을 위한 서술형 평가도구’를 사용하여 과학영재교육원 학생들의 과학 통합 탐구 능력을 평가 요소별, 성별, 이수 과정별, 전공 분야별로 비교하였다. 이 평가도구는 탐구 사고력의 주요 요소들인 탐구 문제 도출, 변인 추출, 가설 설정, 실험 설계, 자료 변환, 자료 해석, 결론 도출, 일반화, 평가에 관한 문항이 각 2개씩 총 18문항으로 구성되어 있다. 이 평가도구를 2013년 현재 C 대학교 부설 과학영재교육원에 다니고 있는 학생들 중 102명 학생들(초등과학반 15명, 중등과학심화Ⅰ반 58명, 중등과학심화Ⅱ반 29명)을 대상으로 시행하였다. 그 결과, 과학 통합 탐구 능력의 요소 중 가설 설정, 변인 추출의 점수는 상대적으로 높았고, 자료 변환, 자료 해석, 평가의 점수는 상대적으로 낮았다. ‘자료 변환’에 해당하는 선 그래프 그리기 문항과 ‘평가’에 해당하는 실험 설계의 개선점 찾기 문항의 정답률은 약 40 %로 상대적으로 낮게 나타 났다. 영재교육을 받은 단계별로 비교하면 과학 통합 탐구 능력의 요소들 중 일반화의 경우 유의미한 차이가 있었다. 남학생과 여학생의 점수 차이는 유의미하지 않았다. 이 연구에서 사용한 평가도구가 생물 분야의 소재를 다루고 있어 생물 분야의 영재 학생들과 다른 분야(물리, 화학, 지구과학)의 영재 학생들과 비교한 결과, 유의미한 차이가 없었다. 과학영재교육원 학생들의 과학 통합 탐구 능력 향상을 위하여 자료 변환, 자료 해석, 평가에 관한 교수·학습을 강화하고, 과학 탐구와 관련된 구체적인 교수·학습 자료를 개발할 필요가 있다. The purpose of this study was to investigate science integrated process skill of the students in science education center for the gifted. In order to do this, ``free-response test for the assessment of science process skills`` developed by Yu-Hyang Kim(2013) was administered to 102 students(15 in elementary school science class, 58 in middle school science classⅠ, and 29 in middle school science classⅡ) who attend the program of science education center for the gifted in C university. The assessment tool measured 9 skills ; formulating inquiry questions, recognizing variables, formulating hypotheses, designing experiment, transforming data, interpreting data, drawing conclusions, formulating generalizations, and evaluating the designed experiments. As a result, the students in science education center for the gifted had relatively high scores in the area of ``formulating hypotheses`` and ``recognizing variables``, but they had relatively low scores in the area of ``transforming data``, ``interpreting data``, and ``evaluating the designed experiments``. The 2 items` percentage of correct answers were below 40% ; one is about a drawing a line graph in ``transforming data``, and the other requires finding improvements of the experimental design in ``evaluation``. There was no significant difference between boys`` scores and girls`s one, and between the scores of students in the field of biology and those of students in the other fields(physics, chemistry, and earth science) in science integrated process skills. And there was significant difference according to the periods receiving the gifted education in ``formulating generalizations``. The teaching and learning has to focus on improving science integrated process skills in the program of science education center for the gifted and teaching and learning materials needs to be developed.

Identification and Diagnostic Utility of Serologic Reactive Antigens from Mycobacterium tuberculosis Sonic Extracts

Kwon, Yu-Mi,Jung, Ki-Hwan,Choi, Go-Eun,Shin, A-Rum,Lee, Byung-Su,Won, Choul-Jae,Kim, Woo-Sik,Shin, Sung-Jae,Park, Jeong-Kyu,Chang, Chul-Hun L.,Kim, Hwa-Jung 대한미생물학회 2009 Journal of Bacteriology and Virology Vol.39 No.4

It is important to identify and to test serologically active antigens, so as to devise a cocktail of the best antigens or peptides. We searched for antigens that have serodiagnostic utility using two-dimensional fractionation of sonic extracts from Mycobacterium tuberculosis and probing with pools of sera from healthy subjects and patients with tuberculosis (TB). Reactive protein spots with patient sera were identified by tandem mass spectrometry. Three proteins, Rv0652, Rv2626c, and Rv3418c, which have not previously been described as serologic targets, were identified. Rv0652 protein among them was expressed in Escherichia coli and serum IgG antibodies against this antigen were measured in 150 patients with pulmonary TB and in 115 healthy subjects. The sensitivity and specificity were 39% and 92%, respectively. These results suggest that a newly identified protein, Rv0652 may be a valuable candidate to be included in a cocktail test kit for TB diagnosis.

Validation and Application of a Qualitative Real-Time Polymerase Chain Reaction Method for Detecting Genetically Modified Papaya line 55-1 in Papaya Products

Yu Jihn Kwon,So Young Chung,Eun Joo Koo,Ji Eun Park,Dong Hyuk Seo,Yo A Lee,Yu Young Jung,Hee Eun Min,Mi Ran Kim,Eungui Kang,Jeongyun Cho,Seong Soo Park,Sun Ok Choi,Chul Joo Lim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Genetically modified (GM) papaya (Carica papaya L.) line 55-1 (55-1), which is resistant to papaya ringspot virus infection, has been marketed internationally. Many countries such as the European Union, Japan, and Korea have a mandatory safety assessment, approval and labeling regulations for GM foods. Thus, there is a need for specific methods for detecting 55-1. In this study, we established a real-time PCR detection method applicable to 55-1 for a variety of papaya products. The limit of detection was possible for fresh papaya fruit up to dilutions of 0.005% and 0.01% (weight per weight [w/w]) for homozygous SunUp and heterozygous Rainbow cultivars, respectively, in non-GM papaya. The 55-1 event-specific detection method observed parallelism (r2>0.99) between the concentration of line 55-1 cultivars and Ct values obtained in amplification plots at concentrations of 0.005-10% for SunUp DNA and 0.01-10% for Rainbow DNA. The method was applicable to the qualitative detection in various types of processed products (cocktail fruit, dried fruit, juice, etc.) containing papaya as a main ingredient. Monitoring papaya products for the presence of GM papaya were demonstrated using a P35S and T-nos real-time PCR detection method but no amplification signals were detected.

Identification and Diagnostic Utility of Serologic Reactive Antigens from Mycobacterium tuberculosis Sonic Extracts

Yu-Mi Kwon,Ki-Hwan Jung,Go-Eun Choi,A-Rum Shin,Byung-Su Lee,Choul-Jae Won,Woo-Sik Kim,Sung-Jae Shin,Jeong-Kyu Park,Chulhun L. Chang,Hwa-Jung Kim 대한미생물학회 2009 Journal of Bacteriology and Virology Vol.39 No.4

Poster Session : PS 0704 ; Rheumatology ; A Case of Seronegative Rheumatoid Arthritis Following Treated Adult-Onset Still`s Disease

( Mi Hye Kwon ),( Go Eun Lee ),( Yu Mi Jo ),( Chung Il Joung ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Adult-onset Still`s disease (AOSD) is an uncommon autoinfi ammatory disease. Typical features include high spiking fever, evanescent rash, and arthritis or arthralgia. Still`s disease is a facet of juvenile idiopathic arthritis, however AOSD and rheumatoid arthritis (RA) are considered unrelated because clinical presentations and hypothesized pathogenesis are different. To our knowledge a case of RA following AOSD has not been reported yet, and we experienced a case so we report it. A 54-year-old man was admitted for one month of fever. Fever was predominant in the afternoon one or two times a day, reaching 39~40℃ and accompanied with pain on ankles. Initial lab showed elevated ESR and CRP, and serum ferritin level was just above normal. Withempiric antibiotics treatment, further evaluations for fever of unknown origin were performed. Bone marrow biopsy was negative, while several reactive lymph nodeswere seen on PET CT. On 16th hospital day serum ferritin was elevated to 1725. 91 ng/ mL and he had new skin rash on his lower legs, he was consulted for rheumatologic evaluation. RF, anti-CCP, ANA, and ANCA were all negative and he was diagnosed as AOSD. With steroid therapy the symptoms and signs were improved. Steroid was tapered and stopped uneventfully within 3 months. After 6 months of the last visit he revisited with painful swelling of both 2nd and 3rd MCP joints, left 2nd PIP joint, and both 3rd and 4th PIP joints. He had no fever or rash, ferritin level was normal. Insidious onset of joint symptoms, typical symmetrical polyarticular involvement of small joints of hands made us diagnose him as seronegative RA. He has been followed on methotrexate.