65세 이상의 노령층에서 고지혈증의 위험요인

김용배,장원기,황보영,김화성,안규동,이병국,이용진,김세훈,김승수,김승훈,김영록,김웅빈,이성수 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

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연쇄중합효소반응을 이용한 태아성별진단

이희섭,김용신,김화선,김정중,김원신 원광대학교 생명공학연구소 1996 생명공학연구소보 Vol.4 No.1

For sex determination by the PCR method, oligoprimers to Y-chromosome gene, DYZ1, SRY, and AMGL were synthesized and genomic DNA was extracted from male and female placenta for the control use. DYZ1 represented 154 bp single band to 0.001pg/ml male genomic DNA but did not represent 154 bp band in female genomic DNA, SRY represented 341 bp band to 1 pg/ml male genomic DNA but did not represent 341 bp band in female genomic DNA, and AMGL represented 977 and 788 bp double band to 1 pg/ml male genomic DNA and 977 bp single band to 1 pg/ml female genomic DNA in 2% agarose gel electrophoresis stained with ethidium bromide. DYZ1 was 1,000-fold sensitive than SRY and AMGL. DYZ1 and SRY could not identify the PCR failure from female but AMGL identified. To increase the sensitivity, the dual amplification of AMGL was performed and the sensitivity increased to 1,000-fold. During the dual amplification of female genomic DNA mixed with male genomic DNA, 0.00125pg/l, 1:400 part male genomic DNA contamination represented double bands as male. In 2 cases of 46, XY female, DYZ1 and AMGL amplification represented male band but SRY amplification did not represent male band. It was suggested that SRY gene was deleted in two 46, XY female cases. for fetal sex determination, PCR with DYZ1, SRY, and AMGL was performed in 10 cases of chorionic villi and 15 cases of amnionic cells. By the comparison with karyotyping result, fetal sex determination was achieved successfully in all 23 samples using PCR of SRY and AMGL but false result was detected in 3 cases(13%) using DYZ1. According to our results, it was concluded that DYZ1 was 1,000-fold sensitive than SRY and AMGL but could not be used because of its false results, and AMGL and SRY must be used concomitantly for precise sex determination.

경련성 환아 부모들의 인식에 대한 연구

이경화(Kyoung Hwa Lee),서은숙(Eun Sook Suh),김영창(Yong Chang Kim),김창휘(Chang Hwi Kim),이동환(Dong Whan Lee) 대한소아신경학회 1996 대한소아신경학회지 Vol.3 No.2

Convulsive disorder is the most part of the pediatric neurologic disease. As far as the treatment of convulsive disorder concerned, we must consider familys' understanding, and the close cooperation between the epileptic child, parents, and doctors. We compared to Kim's report in 1985, to study the change of parents of epileptic children thinking. A prospective study was undertaken the analysis of 100 epileptic children parents visited to the pediatric dept, of Soonchunhyang university hospital from May to July, 1995. Psychological assessment, questionnaires which contains 17 items were given them, and these items were compared to Kims report in 1985 The results were as follows: 1) Parents of epileptic children answered convulsive disorder has influenced to children growth and development( 60.8% in 1985, 65.0% in 1995 ). 2) For asking the causes of children disease, 40.5% of them in 1985 and 50.0% of them in 1995 answered to uncertain, but only 7.8% of them in 1985 and 7.0% of them in 1995 answered to relation with familial inheritance. 3) The feelings at time of the diagnosis were gloomy or uncomfortable: 52.3% in 1985, 38.0% in 1995 and shameful 28.1% in 1985, 9.0% 1995. parents had belived in the epilepsy was treatable disease: 41.0% in 1995. 4) According to the this survey, patients explained reasons why their children take the medication increased from 23.5% in 1985 to 65.0% in 1995. 5) Only family, knew that their children had the convulsive disorder about 50.3% in 1995 and 22.0% in 1995. Family, patients and other people except family knew that their children had the convulsive disorder about 6.5% in 1985 and 43.0% in 1995. 6) The parents opinion and expectation toward the medical doctors were that they wanted to get full explanation for the disease 81.0% in 1985 and 35.1% in 1995, and others were explantation about toxicity and side effects of the medication and advises for the childrens life and activities.

납 노출 근로자들에서 ALAD 및 VDR의 다형질성이 조혈기능 지표에 미치는 영향

이성수,김남수,김진호,김용배,황보영,김화성,안규동,이병국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study was conducted with new workers who entered lead industries from 1992 to 2001 to evaluate the genetic susceptibility of ALAD (δ-aminolevulinic acid dehydratase) and VDR (vitamin D receptor) gene on health effect of lead exposure. Methods: Among the subjects of the database of lead industries at the Soonchunhyang University Institute of Industrial Medicine, only new workers were selected for this study. The total of eligible workers for this category was 3,540 workers, including non lead exposed workers of same lead industries. From stored blood in specimen bank of Soonchunhyang University, genotype of ALAD and VDR were measured using PCR method. Variables for this study were blood lead as an index of lead exposure, ZPP (zinc protoporphyrin in blood), urine ALA (δ-aminolevulinic acid), and hemoglobin as an index of hematopoietic effect of lead. Information on sex, job duration, and weight were collected for personal information. The data were analyzed using SAS (version 8.2) with descriptive analysis of t-test and multiple regression analysis. Results: Among 3,540 new employed study subjects during period of 1992-2001, 3,204 workers (90.5%) had ALAD genotype 1-1, while 336 workers (9.5%) had variant type of ALAD (1-2 or 2-2). For VDR genotype, 2,903 workers (89.7%) out of total tested 3,238 workers were belonged to type bb and 335 workers (10.5%) were type bB or BB. The distribution of genotype of ALAD and VDR were not different according to the job duration in male workers, but were different in female workers. The effect of ALAD and VDR genotype on blood lead were positively significant in the analysis of all cumulative data of new employed workers for 10 years. The effect of VDR genotype on blood lead were stronger than that of ALAD While the variant ALAD gene made decrease of mean ZPP and ALA in urine after controlling for blood lead and other covariate, the variant VDR gene made increased the mean ZPP and ALA in urine in all cumulative data analysis and cross sectional analysis by job duration. For hemoglobin, ALAD and VDR genotype did not affect the mean value. Conclusions: From the above our results, we found that ALAD and VDR genotype exerted significant effect in various way. We confirmed that the finding of a cross sectional study of protective effect of variant ALAD on the effect blood lead on blood ZPP in our retrospective study design. It was found that VDR did not exert protective effect for lead exposure as the variant ALAD did.

축산분뇨 혐기소화 잔재물의 특성 및 초지 적용성 평가

김승민(Seung Min Kim),정웅기(Woong-gi Jung),성용주(Yong Joo Sung),안희권(Hee-Kwon Ahn),김동성(Dong Sung Kim),윤도현(Do-Hyun Yoon),김동섭(Dong-Seop Kim1),정광화(Hwa Gwang Jung) 한국펄프·종이공학회 2014 펄프.종이기술 Vol.46 No.3

보존료가 두부의 저장에 미치는 효과

김동한,신용서,전승호,백승화,이갑상 圓光大學校 1992 論文集 Vol.26 No.2

두부의 저장성 향상을 위해 보존료를 첨가하여 저장중 두부의 미생물상과 이화학적 변화를 검토하였다. 보존료 첨가에 의한 두부의 기호도 변화는 큰 차이가 없었으며 부패가 진행될 수록 적정산도와 amino태 및 ammonia태 질소량은 증가하였다. 두부는 acetic acid로 응고시키는 것이 CaCl₂보다 보존료에 의한 저장성 향상에 효과적이어서, acetic acid에 0.05% K-sorbate와 3% NaCl을 혼합첨가한 구는 저장중 이화학적 변화 및 호기성, 혐기성 세균수가 ?? cell/g수준인 점을 고려해 볼 때 30℃에서 48시간까지 저장이 가능하였다. In order to improve the shelf life of Tofu, the effects of preservatives on Tofu were investigated for microbiol and physicochemical changes during its storage. The sensory evaluation was not deteriorated by preservatives (0.05% K-sorbates). According to the progression of spoliage of Tofu, titratable acidity, and amino and ammonia nitrogen contents were increased. The effect of chemical preservatives on the shelf life of Tofu was shown to be stronger in the Tofu by acetic acid than in that by CaCl₂. The presence of 0. 05% K-sorbate and 3% NaCl as a preservative in Tofu by acetic acid extended it's shelf life upto 48 hrs at 30℃.

골다공증의 위험인자 : 아산시 지역 주민을 대상으로

김용배,장원기,황보영,김화성,안규동,이병국,이용진,이남수,이상건,이상범,이상우,이선정,이성수 순천향대학교 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

Objective : As the average lifespan of human increases, osteoporosis and osteoporosis-related fractures have become major health care problems. Despite recent advances in medical treatment, few studies have assessed the recognition of osteoporosis in general adults. This study examined the recognition of osteoporosis and analyzed the relating factors. Method : A population-based sample of 1086 adults in Asan-city, Korea was investigated with questionnaires and height, weight, Information regarding the general characteristics(gender, age, economic state), lifestylef exercise, smoking, alcohol),medical history(progestin, GH, steroid, calcitonin, PTH), demographic parameter, obesity, stress, and family history was collected through an interview using a structural questionnaire. The level of obesity was measured by the body mass index(BMI). BMD(Bone mineral density) at the lumbar spine and femoral neck was measured by dual energy X-ray absorptiometry. Osteoporosis was considered to be T-score below -2.5. Results : The prevalence of osteoporosis increased progressively with age, decreased with BMI and predominant in women by the result of x^(2) -test, T-test. Age, weight, BMI were significantly correlated with osteoporosis by the result of Pearson correlation ana1ysis(P<0.05). And age, gender, BMI, family history of osteoporosis were proved to be significant risk factors of osteoporosis by the result of multiple logistic regression(P<0.05). Conclusion : These results suggest that age, gender, BMI might be the most important risk factors of osteoporosis. And the history taking about family history of osteoporosis is helpful for diagnosing osteoporosis.

WMI 기술을 이용한 컴퓨터 원격제어 시스템

김정규;김형준;전용화;최병문;서재현;김태공 인제대학교 2012 仁濟論叢 Vol.27 No.1

In organization and company managing a lot of computers, it is difficult to manage the computer system, because it is not easy to recognize exceptions of distributed workplace such as departments and laboratories. Existing computer management systems control many computers through manager using server computer for management in field. Therefore they have the characteristic of strong locality. In this paper, we develop computer remote control system which supports convenient features and non-locality to managers by broadening effective ranges of computer management. We use Web-Site and Android Platform to develop the environment of client management regardless of multiple clients and locality. Moreover, we use WMI (Windows Management Instrumentation) to recognize and control the status of client PC without a impact on computer users.

시화호의 계절변화에 따른 지화학적 환경요인 특성 연구

김태하,박용철,이효진,김동화,박준건,김성준,이미연 한국환경과학회 2004 한국환경과학회지 Vol.13 No.12

Seasonal variation of biogeochemical characteristics was determined in Lake Shihwa from October 2002 to August 2003. When the lake was artificially constructed for the freshwater reservoir in 1988, the development of the strong haline density stratification resulted in two-layered system in water column and hypoxic/anoxic environment prevailed in the bottom layer due to oxidation of accumulated organic matters in the lake. Recently, seawater flux to the lake through the sluice has been increased to improve water quality in the lake since 2000, but seasonal stratification and hypoxic bottom layer of the lake still developed in the summer due to the nature of artificially enclosed lake system. As the lake is still receiving tremendous amount of organic matters and other pollutants from neighboring streams during the rainy summer season, limited seawater flux sluicing into the lake may not be enough for the physical and biogeochemical mass balance especially in the summer. The excess of accumulated organic matters in the bottom layer apparently exhausted dissolved oxygen and affected biogeochemical distributions and processes of organic and inorganic compounds in the stratified two-layered environment in the summer. During the summer, ammonia and dissolved organic carbon remarkably increased in the bottom layer due to the hypoxic/anoxic condition in the bottom layer. Phosphate also increased as the result of benthic flux from the bottom sediment. Meanwhile, dissolved organic carbon showed the highest value at the upstream area and decreased along the salinity gradient in the lake. In addition to the sources from the upstream, autochthonous origin of particulate organic carbon from algal bloom in the lake might be more important for sustaining aggravated water quality and development of deteriorated bottom environment in the summer. The removal of trace metals could be attributed to scavenging by strong insoluble metal-sulfide compounds in the hypoxic/anoxic bottom layer in the summer.

질소분압에 따른 TaNx coating층의 미세조직 연구

김선화,최용락 순천향대학교 부설 산업기술연구소 1998 순천향 산업기술연구소논문집 Vol.3 No.3

TaNx film were fabricated for this research by the reactive magnetron sputtering process. In order to improve TCR, it was investigated the transformation of coated TaNx film. The variables were the N₂/Ar fraction and the thermal heat-treatment. The electrical resistance and the TCR of specimen were measured. It was observed the microstructure change under thermal heat-treatment by XRD experiment. Also the morphology of TaNx film was observed by SEM. As a result, TCR was stabilized when N₂/Ar fraction were 0.166 to 0.25. The phases of as-coated TaNx film were Ta, TaN, β-Ta and amorphous. The new phases, Ta₄N and Ta₃N?? were formed after thermal heat-treatment. As the ratio of nitrogen gas was increased, the morphology of the layer was changed to a discontinuous island particle. These results were consistent with results of electrical resistance change.