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      • 실험적 간암 발생 과정에 출현하는 난원형 세포의 면역 조직화학적 연구

        황택근,이중달,원치규 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.1

        Oval cells have been observed during chemical carcinogenesis in the livers of animals fed 2-acetylaminofluorene (2-AAF), a potent chemical carcinogen. Biological behavior of these cells remained poorly understood with regard to gepatic carcinogenesis. The author carried out the histological, enzyme histochemical and immunohistochemical studies of oval cells in the livers from 80 rats fed 2-AAF for five months. Throughout the experimental period oval cells along with hepatocytes and bile ductular cells were subjected to immunostain for monoclonal antibodies. The antibodies included those to carcinoembryonic antigen(CEA), alpha-fetoprotein(AFP), low molecular cytokeratin(CAM 5.2), prealbumin, and endothelial cells. Alkaline phosplatase(ALP) reaction to oval cells was also evaluated. The followings were results obtained: Oval cells appeared about the portal areas at the 10th day of the experiment. The cells penetrated the hepatic lobule as they proliferated. The proliferation of oval cells was most prominent at the 6th week and then the cells were gradually decreased in number until the late stage of the experiment. There were foci of transformation of oval cells to cholangiolar cells and foci of cholangiofibrosis made up of proliferated bile ductules. PAS reaction was negative on oval cells, where as hepatocytes were reactive for PAS. ALP reaction was positive on both oval cells and ductular epithelial cells. The hepatocytes were non-reactive for ALP. Both oval cells and bile ductular cells showed positive staining for AFP and CAM 5.2, and negative for CEA and prealbumin. Hepatocytes showed negative staining for AFP, CAM 5.2, CEA and prealbumin. However, an impressive finding that AFP- and CAM-positive hepatocytes were scattered through the hepatic lobules, was observed early during hepatocarcinogenesis. The results indicated that oval cells had no evidence of the conversion to hepatocytes in 2-AAF induced hepatocarcinogenesis, and that hepatocellular carcinoma arose not from oval cells. Oval cells showed immonohistochemical characteristics similar to those of the bile ductular epithelium.

      • Wafer final polishing에 따른 표면 현상과 마멸도에 관한 연구

        원종구,이정택,황성철,차지완,이은상 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

        In recent years, developments in the semiconductor and electronic industries have brought a rapid increase in the use of silicon wafer. For further improvement of the ultra precision surface and flatness of Si wafer necessary to high density ULSI, it is known that final polishing is very important. Polishing one of the important methods in manufacturing of Si wafers and in thinning of completed device wafers. This study will report the evaluation on abrasion of wafer according to variety processing condition, which have major influence on the abrasion and surface defect of Si wafer polishing, were adapted to polishing pressure, machining speed, and the slurry mix ratio. Thus the optimum condition selection of ultra precision Si wafer polishing using load cell and infrared temperature sensor. To evaluate each machining factor, use a data through each sensor. That evaluation of abrasion according to variety condition is selected to use a result data that measure a pressure, machining speed, and the processing time. And optimum condition is selected by this result. By using optimum condition, it achieve a ultra precision mirror like surface.

      • 수용액중에서 아세틸피리딘류의 해리에 미치는 온도와 압력의 영향

        黃正儀,鄭在元,鄭載植 慶北大學校 1990 論文集 Vol.49 No.-

        The dissociation constants of 2-, 3- and 4-acetylpyridine were measured by conductometric method in the temperature range from 15 to 40℃ and pressure up to 2,500bar. The dissociation constants of these substituted pyridines were increased as the temperature increase but decrease as the pressure. From the data thermodynamic properties were calculated and the Hammett plat were obtained. From these properties the characteristic properties of the dissociation reaction were discussed.

      • 코스틱스법에 의한 순수 전단장치 평가에 관한 연구

        황재석,김원현,서정희 嶺南大學校 工業技術硏究所 1998 工業技術硏究所論文集 Vol.26 No.1

        There are many experimental devices for pure shear test such as off-axis tension test pure shear test for Iosipescu conceptions. Arcan circular disk for Arcan conceptions and the general pure shear test and Hawong Iosipescu shear test(H.I.S.T.) etc.. Hawong Iosipescu shear test(H.I.S.T.) device is analyzed through caustics. It is assured that H.I.S.T. device is more effective than Iosipescu shear test(I.S.T.) device for the measurement of stress intensity factor from causic. It is known that H.I.S.T. device without notch is most effective as pure shear test device, between H.I.S.T. device and I.S.T. device for caustics, but H.I.S.T .device and I.S.T. devices are can be used ad pure shear test device

      • KCI등재

        흉골 골절의 의의에 관한 임상적 연구

        원형섭,황성연,박정배,이준형,박철완,이근 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

        It has been known that sternal fracture increases the risk of potentially life-threatening injuries, such as myocardial, pulmonary, and thoracic vascular injuries, and it has 25% to 45% of mortality, primarily resulting from these underlying injuries. The authors reviewed retrospectively the medical records and radiographic findings of the patients who admitted with sternal fracture at Chung Ang Gil Hospital from 1 June 1994 to 31 December 1995 to evaluate the significance and clinical course of sternal fracture. The results were followings: 1. The male to female ratio was 3:2 and the mean age was 43.1 and 45.4 years old in male and female, respectively. 2. Motor vehicle occupant accident was the most common cause of sternal fracture. 3. Of 33 cases, there was no death, but common associated injuries which were rib fracture, hemothorax, lung contusion, vertebral fracture, penumothorax, long bone fracture, myocardial contusion, flail chest, facial chest, facial bone fracture, and pericardial effusion, in order. 4. The clinical course of sternal fracture was determined primarily by associated injuries except for communited sternal fracture. 5. The patients with isolated sternal fracture, who had an otherwise normal chest x-ray, normal electrocardiogram, no other significant associated injuries and are hemodynamically stable, had a benign clinical course.

      • SCIESCOPUSKCI등재
      • SCOPUSKCI등재

        Pseudomonas sp. JH007에 의한 DL-2-Chloropropionic Acid로부터 D-Lactic Acid의 생산

        정자헌,황인균,방원기 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

        DL-2-chloropropionic acid로부터 D-lactic acid를 생산하기 위하여, 토양으로부터 DL-2-chloropropionic acid를 유일한 탄소원 및 에너지원으로 이용할 수 있는 균주 80여종을 분리하였으며, 분리균주들로부터 DL-2-chloropropionic acid로부터 D-lactic acid 생산성이 우수하며, L-lactic acid를 생산성이 우수하며, L-lactic acid를 생산하지 않은 균주 JH-007을 선별하여 Pseudomonas sp.로 동정하였다. DL-2-chloropropionic acid로부터 D-lactic acid를 생산하기 위한 최적 조건을 조사하기 위하여 3 g/l의 DL-2-chloropropionic acid가 포함된 LB 배지에서 배양하여 수확한 후 수확된 균체를 효소원으로 사용하였다. D-lactic acid 생산시 최적 반응액 조건은 125 mM sodium carbonate buffer에서 휴지균체 10 g/l와 3 g/l의 DL-2-chloropropionic acid를 사용할 때였으며, 최적 반응 pH는 10.0, 최적반응 온도는 30℃이었다. 최적조건하에서 반응액에 1 g/l의 DL-2-chloropropionic acid를 간헐적으로 첨가하여 5시간 반응시켰을 때 5.72 g/l의 D-lactic acid가 생산되었으며, 전환율은 98.4%였으며, 광학순도는 99.8%이었다. For the production of D-lactic acid from DL-2-chloropropionic acid, about 80 strains of bacteria capable of assimilating DL-2-chloropropionic acid as a sole carbon and energy source were isolated from the soil. JH-007 strain that showed the higest productivity of D-lactic acid and didn't produce L-lactic acid from DL-2-chloropropionic acid was selected from them and identified as Pseudomonas sp. The optimal conditions for the production of D-lactic acid from DL-2-chloropropionic acid were examined. The resting cells of JH-007 cultured in LB medium containing 3 g/l of DL-2-chloropropionic acid were used as an enzyme source. The reaction mixtures for the maximal production of D-lactic acid were consist of 10 g/l of resting cells and 3 g/l of DL-2-chloropropionic acid in 125 mM sodium carbonate buffer. The optimal pH for the reaction was 10.0 and the optimal temperature was 30℃. When 1 g/l of DL-2-chloropropionic acid was added intermittently to the reaction mixture under the above condition, 5.72 g/l of D-lactic acid was produced after incubation of 5 hrs. This amount of D-lactic acid corresponded to a 98.4% yields and the optical purity was 99.8%.

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