Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

경도 및 중등도 본태성 고혈압환자에서 Amlodipine단독요법

안기완,서영환,김상도,박윤환,김철,조경상,김완중,홍순표 최신의학사 1992 最新醫學 Vol.35 No.9

共同住宅 中央 集中 煖房設備의 信賴度에 관한 硏究

김동완,정순성,조용수 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.1

The purpose of this study is to obtain reliability of heating equipments in apartment building. Field data concerning the failure of heating equipments recorded at the apartments constructed 14 years ago are used. As a result of the field data analysis, failure time distribution and cumulative hazard function of boiler, heat exchanger, steam header and various pumps are expressed in Weibull probability graph. The scale and shape parameters, MTBF of each equipments are presented by regression line of Weibull probability graph. Presented parameter wi31 be come into use a reference data to evaluate the reliability, to plan the maintenance scheme, and to evaluate economic renewal of equipments.

치수생활력 검사를 위한 Laser Doppler flowmeter의 이용

김영진,남순현,김현정,서완종 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.3

저자는 본 병원에 내원한 환자중 미완성치구근단을 가지고 있으며 외상받은 치아 및 자가이식치아를 대상으로 기존의 치수생활력 검사방법과 더불어 LDF를 이용한 검사방법을 통해 다음과 같이 요약할 수 있다. 1. 치수 생활력 유무를 판단함에 있어 현재까지 널리 사용된 전기치수검사, 냉검사등의 방법은 치수 내 신경발달 정도 및 피검자의 주관에 의해 반응이 다르게 나타나는 경우가 많다. 2. 이에 반해 LDF를 이용한 치수생활력 검사방법은 위음성 및 위양성 반응의 빈도가 낮아 신뢰도가 높으며 비교적 조기에 치수생활력을 특정할 수 있는 우수한 방법이다. Various diagnostic methods, such as electric pulp test, cold test, cavity etc, have been used to determine the pulp vitality. At least two or more methods should be used with supplemental information to diagnose each case because using one method doesn't provide sufficient accuracy. These conventional methods have depended on nervous responses, but laser Doppler Flowmeter determines the pulp vitality by measuring pulpal blood flow. In this case, we tested the vitality of the traumatized teeth with incomplete root apices and autotransplanted teeth with conventional methods and Laser Doppler Flowmeter . Conventional methods. such as electric pulp test and cold test can produce various responses according to the development state of the pulpal nerve and the sensory threshold of the patient. but ,laser doppler Flowmeter has a lower false response rate than other pulp vitality test methods and it can determine the vitality somewhat earlier than the other method.

共同住宅 中央 集中 煖房設備의 信賴度에 관한 硏究

김동완,정순성,조용수 東亞大學校 建設技術硏究所 1997 硏究論文集 Vol.21 No.1

The purpose of this study is to obtain reliability of heating equipment’s in apartment building. Field data concerning the failure of heating equipment’s recorded at the apartments constructed 14 years ago are used. As a result of the field data analysis, failure time distribution and cumulative hazard function of boiler, heat exchanger, steam header and various pumps are expressed in Weibull probability graph. The scale and shape parameters, MTBF of each equipment’s are presented by regression line of Weibull probability graph. Presented parameter will be come into use a reference data to evaluate the reliability, to plan the maintenance scheme, and to evaluate economic renewal of equipment’s.

대동맥 이첨판과 승모판 이탈증을 동반한 대동맥 판하부 협착 1예

김용화,장경식,양태영,임영국,안기완,홍순표 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Discrete subaortic stenosis is a rare cardiac disorder especially in Orientals which occupies eight to ten percent of aortic stenosis, It occurs mainly before age of eighteen and accompanies with aortic valve abnormalities such as aortic stenosis, supravalvrlar stenosis, bicuspid valve and muscular hypertrophic subaortic stenosis, and other cardiac abnormalities such as ventricular septal defect, pulmonary stenosis and coarctation of aorta. There is no report of discrete subaortic stenosis accompanied with mitral valve prolapse. The major complications of discrete subaortic stenosis are aortic insufficiency, and subacute bacterial endocarditis. We report a case of asymptomatic nineteen-year-old male of discrete subaotic stenosis with bicuspid aortic valve and mitral valve prolapse, which is comfirmed by two-dimensional and Doppler echocardiography including transesophaigeal echocardiography.

제6차 교육과정에 따라 편찬된 초등학교 자연 교과서의 분석(Ⅱ)

김광명,장병기,이대형,신순임,강태완 춘천교육대학교 과학교육연구소 1997 과학교육연구 Vol.20 No.-

CORBA 클라이언트/서버 구조의 자바 통합 개발 환경의 설계 및 구현

김완수,김흥준,박순규 진주산업대학교 2000 산업과학기술연구소보 Vol.- No.7

Today, most of the well-known software development tools and methods faced with problems of scalability, modularity, granularity, and maintenance. These problems result in the fact that these tools and methods are standalone. In this paper, we propose a design and implementation of software development environment for Java application based upon CORBA-based Client/Server model. Proposed model has facilities to overcome a problem caused by updates and its distribution and is possible to overcome problems of scalability, modularity and granularity because it based upon Client/Server model.

자연 교과 지도에 대한 초등 교사의 인식

김광명,신순임,강태완,이대형,장병기 춘천교육대학교 과학교육연구소 1995 과학교육연구 Vol.18 No.-

Mouse의 치아 발육시 Runx2의 발현 양상

김태완,류현모,남순현,김영진,김현정 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.4

Runx2는 runt gene family에 속하는 전사조절 인자로써 뼈의 형성과 골아세포의 분화에 중요한 역할을 담당하고 있다. Runx2-haploinsufficency는 쇄골의 저형성 및 두개 봉합의 지연을 특징으로 하는 쇄골두개 이형성증을 일으키며, 치아에 있어서는 법랑질의 저형성, 영구치 맹출지연 등을 보인다. 이에, 치아의 발육 및 맹출에 미치는 Runx2의 영향을 알아보기 위해 in situ hybridization 방법으로 태생 1, 4, 7, 14. 21일 된 쥐의 하악 및 제1대구치를 사용하여 실험을 실시하였다. Runx2-full length는 태생 1일과 4일에 치낭 및 주위조직에 보이지만 Runx2-typeⅡ는 보이지 않았다. Runx2-full length는 태생 7일에 치관 교합면 부위의 법랑모세포에 발현하였고, 1주일 후인 태생 14일에는 백악법랑경계 상방의 치관인 접면 법랑모세포에서 발현되었다. 이에 반해 Runx2-typeⅡ는 법랑모세포에서 발현하지 않았다. 또한 태생 21일에서는 두가지 이성질체가 모두 하악골에서 발현을 보였다. 이런 결과를 종합해볼 때, Runx2-full length는 치아의 맹출과 연관이 있으며, 법랑모세포의 분화 및 이로 인한 법랑질형성에 영향을 주지만 Runx2-typeⅡ는 하악골의 형성에만 영향을 미치는 것으로 사료된다. Runx2 is a transcription factor in homologous with Drosophila runt gene and it is essential for bone formation during embryogenesis and a critical gene for osteoblast differentiation and osteoblast function. Runx2-haploinsufficency causes cleidocranial dysplasia (CCD). CCD is an autosomal-dominant inherited disorder characterized by hypoplastic clevicle and delayed ossification in fontanelles and wormian bones. Dental defects are possibly shown to CCD patients : multiple supernumerary teeth, irregular and compressed permanent tooth crowns, hypoplastic and hypomineralized defects in enamel and dentin, an excess of epithelial root remnants, the absence of cellular cementum, and abnormally shaped roots. In addition, delayed eruption of the secondary dentition is a constant finding. The aim of this study is to evaluate the role of Runx2 in the tooth development and eruption through analyzing the expression pattern of Runx2 by in situ hybridization during crown (late bell stage) and root formation of tooth, using postnatal day 1, 4, 7, 14 and 21 mice mandibular molar teeth. mRNA of Runx2-full length is expressed in dental follicle and surrounding tissue at postnatal day1 and 4. At post-natal day 7, it is expressed in ameloblasts of occlusal surface of enamel and bone area surrounding the tooth. In comparison with previous stage, at postnatal day 14, it is expressed in ameloblasts of proximal surface of enamel. At postnatal day 21 it's expression is observed only in bone area. mRNA of Runx2-typeⅡ is not expressed At postnatal day 1 and 7. At postnatal day 14 and 21, it's expression is observed in the bone area. In this study, we suggest that Runx2 have a relation of ameloblasts differentiation and an important role to tooth eruption made by dental follicle during intraosseous eruption stage. Also we can confirm that Runx2 has a role to bone formation.