Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

실용음악 전공 학생들의 발성치료 전후 성대 및 음성 특성 비교

황연신,심미란,김철준,최용석,김상연,최찬호,선동일,Hwang, Yeon Shin,Shim, Mi Ran,Kim, Chul Jun,Choi, Yong Suk,Kim, Sang Yeon,Choi, Chan Ho,Sun, Dong Il 대한후두음성언어의학회 2016 대한후두음성언어의학회지 Vol.27 No.2

Background and Objectives : Many students major in applied music singing have various difficulties in phonation when they sing or perform. But the studies about this cause are lack. The purpose of this study is whether singing voice therapy based on vocal music is effective or not to applied music singing students that have difficulties in phonation. Materials and Methods : Singing voice therapy program had been done to 8 students major in applied music singing during 7 weeks. We did laryngeal stroboscope, perceptual, acoustic and aerodynamic examination before and after singing voice therapy to this students. And we studied post examination results have differences or improvements compared to pre examination results. Results : Four male students don't have effective improvement after singing voice therapy but they shows slight improvement in aerodynamic examination values compared to pre examination values. Four female students shows improvement in aerodynamic examination values and pitch range increasing. Above all, one student's vocal nodules disappeared after singing voice therapy. Conclusion : Singing voice therapy based on music singing is very effective to the students major in applied music singing. Above all, their curriculum in applied music singing should include phonation training based on music singing before training perform and technic practices.

공정 조절에서 이상원인의 제거 방법에 대하여

전상표 남서울대학교 2005 남서울대학교 논문집 Vol.11 No.2

Assignable causes producing temporary deviation from the underlying system can influence on process adjustment and process monitoring in dynamic feedback control system. In this paper, Chapter Ⅱ will summarize the methods of readjusting unadjusted disturbance when assignable causes occur in dynamic process adjustment, and of removing the causes of assignable causes.

기저질환이 없는 사람에서 발생한 Campylobacter fetus에 의한 재발성 심내막염 1예

전재범,정진원,김성혜,송문희,오형철,안성기,최상호,김남중,김양수,우준희,류지소 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

C. fetus 심내막염은 드물게 발생하는 질환으로 주로 면역능이 저하된 환자에서 발병하고 대동맥판을 침범하는 경우가 많으며 적절한 치료가 되지 않을 경우 치사율이 높다. 저자들은 기저질환이 없던 69세 남자에서 C. fetus 심내막염을 경험하였기에 문헌고찰과 함께 보고하는 바이다. 환자는 발열로 내원하여 시행한 혈액배양 검사상 C. fetus가 동정되었고 경식도 초음파 검사상 삼첨판에 증식증이 발견되어 심내막염 진단하에 ceftriaxone에 이어 meropenem으로 총 4주간 항생제를 투여받고 호전되었다가 한달 후 다시 재발하였고 판막부전 소견 진행하여 삼첨판 치환술을 시행받고 전신상태 호전되어 퇴원하였다. Campylobacter fetus is a rare cause of endocarditis. To date, only about 30 cases of C. fetus endocarditis have been reported mainly in the elderly with chronic underlying illnesses. Most cases showed tendency to involve aortic valve. This report describes a case of native tricuspid valve infective endocarditis in a 69-year-old man without any underlying disease. Despite adequate antibiotic therapy for 4-weeks, endocarditis recurred and leading to surgical valve replacement.

홍경희와 도시지리학

성준용 한국도시지리학회 2002 한국도시지리학회지 Vol.5 No.2

본고는 洪慶姬(1919. 5. 8 - 2001. 6. 6) 선생이 한국의 도시지리학의 초창기에 도시지리학의 발달에 어떻게 공헌하였는가를 그녀의 연구업적을 토대로 고찰한 것이다. This paper aims to investigate the academic contribution of professor Kyung Hi Hong to the development of the Korean urban geography through her extensive studies. By investigation her studies it turned out that her studies were the earliest and the largest in their number in the early stage of urban geography. In particular, her articles were proved to be outstanding in the five parts of urban geography such as the study of the location, distribution and size of the city, urbanization, urban function, urban residential pattern, and central place except the studies of the urban system.

우리나라 국고보조금제도의 합리적 개선방안

전상경 東亞大學校 1999 東亞論叢 Vol.36 No.-

National subsidy is an important financial resource for local government. However, different from two other grants-in-aids i.e., local shared tax and local transfer, it is mainly distributed by central government's political judgement. Therefore there has been a lot controversies over the rational administration of the national subsidy system, and many reform proposals for the national subsidy system have been suggested. This paper examines why such proposals have not been implemented successfully, clarifies the inherent natures of Korean national subsidy system, and suggests another directions for the rational management of subsidy system within the given inherent political-administrative constraints.

Au를 공침한 SnO₂후막센서의 가스 감응 특성

성정훈,강대원,이윤수,임준우,홍영호,이덕동 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

High selective and sensitive thick film type methane gas sensors were fabricated and their sensing characteristics for various hydrocarbon gases were investigated. Raw material, SnO_(2)/Au, prepared by coprecipitation was even grain size. SEM, TEM, BET and XRD analyses were carried out for investigation of surface morphology and crystalline structure. The thick film devices using the above materials exhibited high sensitivity to methane gas at the operating temperature of 400°C. The sensitivity of SnO_(2)/Au+Pd thick film devices to methane gas was higher than that to the other hydrocarbon gases such as iso-butane and propane. The optimal adding amounts of Au and Pd were 1wt.% and 3wt.%, respectively. The sensitivity, S, is defined as {(R_(A)-R_(G))/R_(A)}><100, which R_(A) and R_(G) are resistance in air and that in the gas ambient, respectively.

교육에 있어서 개인차의 반영과 수준별 교육과정

전성연 고려대학교 교육문제연구소 1997 敎育問題硏究 Vol.9 No.-

구조용 중탄소강 SM45C의 열처리에 따른 기계적 성질변화

전상조,이임균,김송희 江原大學校 産業技術硏究所 1986 産業技術硏究 Vol.6 No.-

The aim of this study is to find out the relationships between the microstructures of SM45C(AIS11045) steel and fatigue crack propagation behaviour. Three microstructures such as (ⅰ) as received (fully annealed). (ⅱ) water quenched and tempered, and (ⅲ) ail quenched and tempered were used for fundamental mechanical testing and fatigue crack proagation test. The microstructures of (ⅱ) and (ⅲ) showed superior in tensile strength to (ⅰ). Resistance against fatigue crack propagation was higher in structure (ⅰ), while tensile properties were better in structures(ⅱ) and (ⅲ). It is believed due to that the enhancement of roughness of fracture surface obsered in structure (ⅰ) increases ?Kth and lowers fatigue crack growth rate. However it does not necessarily mean the quenched and tempered structures (ⅱ) and (ⅲ) are undesirable for the engineering component because fatigue limit in low cycle test appears usually higher in the microstructures of higher strength.

SAS CALIS 절차를 이용한 효과적인 구조방정식 모형의 사용

전성해 청주대학교 산업과학연구소 2008 産業科學硏究 Vol.25 No.2

We propose an efficient usage of structural equation model using SAS CALIS procedure in this paper. There have been many softwares of structural equation model, such as, RISREL, AMOS, and so forth. These are exclusive softwares for structural equation model. As the case may be, we need other statistical analysis functions as well as structural equation model. But, traditional softwares for structural equation model are insufficient as general statistical analysis tools. So, in this paper, we use SAS system for structural equation model and additional statistical modeling. The SAS system has CALIS procedure for structural equation model. Also, it has many tools for general statistical analysis.