Anti-Inflammatory Effect of By-Products from<i>Haliotis discus hannai</i>in RAW 264.7 Cells

Rho, Ho-Seok,Kim, Hyang,Kim, Jung-Ae,Karadeniz, Fatih,Ahn, Byul-Nim,Nam, Ki-Ho,Seo, Youngwan,Kong, Chang-Suk Hindawi Limited 2015 Journal of chemistry Vol.2015 No.-

<P>Several reports promoted the potential of shellfish due to their ability to act as antioxidant, anti-inflammatory, and antimicrobial agents. Pacific abalone,<I>Haliotis discus hannai</I>viscera is, reported to possess bioactivities such as antioxidative stress and anti-inflammatory. In this study, anti-inflammatory potential of mucus-secreting glands from shell-shucking waste of<I>H. discus hannai</I>was evaluated using RAW 264.7 mouse macrophage cell model. Results indicated that presence of<I>H. discus hannai</I>mucosubstance by-products (AM) significantly lowered the nitric oxide (NO) production along the expressional suppression of inflammatory mediators such as cytokines TNF-<I>α</I>, IL-1<I>β</I>, and IL-6 and enzymes iNOS and COX-2. Also, AM was shown to increase expression of anti-inflammatory response mediator HO-1. Presence of AM also scavenged the free radicals<I>in vitro</I>. In conclusion, by-products of<I>H. discus hannai</I>are suggested to possess notable anti-inflammatory potential which promotes the possibility of utilization as functional food ingredient.</P>

한국인의 식품 영양조사 : 제3보

安富浩,柳彦浩,鄭碩敎,金乙祥,洪性愛,徐德揆 한림대학교의료원 1977 人間科學 Vol.1 No.1

토끼 눈물샘에서 Nitric Oxide Synthase의 면역조직화학적 연구

박경호,이석호,고정식,안의태,박대균 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Endogenous nitric oxide(NO) has been known to regulate many physiological and pathological process, especially the salivary secretion and glandular blood flow. However, nitric oxide synthase(NOS) responsible for NO synthesis has not been well studied in rabbit lacrimal gland. The present study was performed to investigate the distribution of nitric oxide synthase isoforms(endothelial, neuronal, and inducible NOS). Immunohistochemistry, using monoclonal mouse anti-endothelial NOS, anti-neuronal NOS, and anti-inducible NOS, was performed in inferior lacrimal of the rabbit. Endothelial NOS(eNOS)-positive immunoreactivities were observed in arterial endothelia, intralobular ducts and interlobular ducts of the inferior lacrimal gland of the rabbit. eNOS-positive immunoreactivities were most prominent in the interlobular and intralobular ducts. Immunoreactivities were well concentrated in cytoplasm of columnar epithelial duct cells. However, weak eNOS-positive immunoreactivity was observed in the intercalated ducts and vascular endothelial cells. Neuronal NOS(nNOS)-positive immunoreactivity was seen in the nerve fibers of inferior lacrimal gland. nNOS-positive immunoreactivy was not detected in duct systems or in acinar cells. Inducible NOS-positive immunoreactivity was not seen. Especially, the expression of the nNOS in the nerves of lacrimal gland suggest that NO may play a role in modulating tear production. These results reveal the presence of eNOS and nNOS in the lacrimal gland of the rabbit, which may be related with regulation of the glandular secretion and blood flow through the gland.

수증기 개질반응용 니켈촉매에 첨가된 리튬의 효과

최재석,윤정숙,임태훈,홍성안,이호인 한국공업화학회 2005 응용화학 Vol.9 No.1

In the direct internal reforming type molten carbonate fuel cell, alkali carbonates used as electrolyte in the cell are transported to reforming catalyst of which activity drastically decreases. In our previous work, the deactivation of the nickel catalyst was studied with increasing the amount of added lithium. Unexpectedly, the increase of activity was observed with a small amount of lithium less than 1wt%. The results of TPR, XRD and chemisorption showed no significant difference between the fresh catalyst and the Li-added one, implying that lithium did not cause the change of morphological property of the supported nickel. To elucidate the effect of lithium the methanation of CO was applied to the catalysts, which was considered as the reverse reaction of the key elementary step of methane steam reforming. It can be seen by an online mass spectrometer that the small injection of CO/H₂ mixture into the Li-added catalyst reactor at 650 ℃ showed the longer tail of produced methane than into the fresh one. The delay should be originated from the effect of lithium, which determined the overall rate on the Li-added catalyst.

球形 글라스비즈의 흐름角 測定

姜錫浩,宋希準,安熙官 嶺南大學校 工業技術硏究所 1978 연구보고 Vol.6 No.1

In order to study the flow characteristics of spherical glass-beads ranging from 40 mesh to-115 mesh, its angle of repose and angle of rupture were measured as a function of average particle size, addition of fine powder as glidant or lubricant, and admixture of 2 or 3 adjacent fractions of beads classified by screening. The angle of repose of the glass-beads increases with increasing particle size of above 100 mesh, but for beads smaller than 100 mesh, the angle of repose increases with decreasing particle size. Fine glidant of montmorcalite added to the beads in very small concentrations of 0.6wt, % decreases the flow angles, while it tends to increase strongly the flow angles at higher concentrations.

니켈담지촉매의 제조과정에서 액상산화의 효과

최재석,임태훈,홍성안,이호인 한국공업화학회 2004 응용화학 Vol.8 No.1

The deactivation of reforming catalyst in the DIR-MCFC was reported to be affected by dispersion, loading amounts, kind of support and so on. In this research, we checked the resistivities of catalysts prepared by the different methods, and examined the effect of liquid phase oxidation. The oxidized catalyst in a liquid phase showed the increased activity and dispersion even though the nickel species was supported by deposition-precipitation method. In order to investigate the role of the oxidation, TGA, TPR and XRD analyses were conducted. The increase in activity was ascribed to the strong metal-support interaction which was induced by liquidphase oxidation.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1994)

김재홍,안진균,정성재,김영태,김중환,김시영,이석종,이홍렬,서호석,김경훈,권혁진,정우권,고우석,이용석,안필수,오준규,오용섭 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

Background : In recent years gonorrhoea has been panendemic and remains one of the most common Sexually Transmitted Diseases in the world, especially in developing countries. Objective & Method : For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria Gonorrhoeae(PPNG), Ive have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1994, 168 strains of N. gonorrhoeae were isolated, among which 109 (64,9% ) were PPNG. Conclustion : Our results suggests that the prevalence of PPNG in Seoul is still increasing.

자가 말초혈액 조혈모세포 이식술의 임상적용

김혁,안명주,오석중,이영열,김인순,정태준,최일영,오미란,임호준,이항,김신규 한양대학교 의과대학 1999 한양의대 학술지 Vol.19 No.1

Responses to chemotherapy correlate with the increased dose of chemotherapeutic agents in some cancers, e.g. breast cancer, Ewing's sarcoma, Hodgkin's disease(HD) and non-Hodgkin's lymphoma(NHL), then for the improvement of the chemotherapy responsiveness, high-dose chemotherapy(HDCT) was proposed. But, it's application was limited due to complications, especially bone marrow suppression. HDCT and succeeding autologous peripheral blood stem cell transplantation(APBSCT) was introduced to overcome this problem. This study was designed to determine the clinical pictures including clinical parameters and the responsiveness of HDCT followed by APBSCT in Hanyang University Hospital. Ten patients were enrolled. They were 8 adults (3 breast cancers, 3 multiple myelomas, 1 HD, 1 NHL) and 2 children (1 acute myelogenous leukemia (AML), 1 neuroblastoma). Mobilization chemocherapy(MCT) followed by Granulocyte Colony Stimulating Factor(G-CSF) was administered to mobilize stem cells from bone marrow to peripheral blood. The stem cells were collected by using CS-3000 and cryopreserved at -196℃ with programmed controlled rate freezer as the mixture with 10% dimethyl sulfoxide(DMSO). HDCT was administered and cryopreserved peripheral stem cells were infused on day 0 as planned. Patients' mean age was 41 years old. After induction chemotherapy three patients achieved complete response and 5 partial response. The median time for bone marrow recovery after MCT was 11.5 days for neutrophil(〉500/㎣) and 4.8 days for platelet(〉20,000/㎣). There were three cases of neutropenic fever and one case of gingivitis. After HDCT with APBSCT, one patient was converted partial response to complete response, and one patient achieved complete response after HDCT without induction chemotherapy. The median time for bone marrow recovery was 12.7 days for neutrophil(〉500/㎣) and 14.7 days for platelet(〉20,000/㎣). Eight patients developed neutropenic fever and required systemic antibiotics. Transfusion was required 1.6 pints for packed red cell and 23.4 pints for platelet concentrates. Toxicity consisted mainly of vomiting, diarrhea, and mucositis which were mild. HDCT with APBSCT can be performed safely with minimal complications. For the evaluation of the exact role of HDCT with APBSCT in malignant diseases, further studies are required as a large scale of patients and lung-term follow up.

할로겐화 탄화수소의 흡착제거기술 Ⅱ. Pressure Swing Adsorption 공정에 의한 할로겐화 탄화수소/공기 혼합물의 분리

이태진,안희관,김윤갑,강석호 嶺南大學校 環境問題硏究所 1990 環境硏究 Vol.10 No.1

An experimental study was performed for the bulk separation of halogenated hydrocarbons and air mixture(CF₂Cl₂/air, CH₂Cl₂/air=0.1/99.9 Vol.%) in a pressure swing adsorption (PSA) process packed with various adsorbents, The PSA process cycle comprised four stes : I pressurizaton, II adsorption, III countercurrent depressurrzation, IV purge. The order of appearance of break-through curve is MS-5A > MS-13X > W-AC > S-AC for CF₂Cl₂/Air and W-AC > S-AC > MS-13X for CH₂Cl₂/Air respectively. The separation effects for the variation of adsorption pressure were investigated. In a steady state PSA cycles, high purity air from step II and CFCs-rich gas from step IV were obtained.

악성 질환에서의 고용량 화학요법과 자가말초혈액 조혈모세포이식술 : 단일기관 치료 경험

최정혜,안명주,오호석,이웅수,오석중,이영열,최일영,김인순 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.2

연구배경: 고용량 화학요법은 항암제의 투여 용량에 따라 항암효과가 비례하는 용량반응 관계가 있는 악성종양에서 효과를 기대할 수 있으며 현재 급성백혈병, 악성림프종, 다발성골수종 등의 혈액 종양과 유방암, 고환암, 난소암, 신경아세포종 등의 고형암 환자에서 시행되고 있다. 저자들은 고용량화학요법에 이어 자가조혈모세포이식을 시행 받은 악성 종양 환자를 대상으로 치료효과 및 부작용 등의 임상상을 알아보고자 하였다. 방법: 1997년부터 2003년까지 한양대학교 병원에서 자가조혈모세포이식을 시행 받은 악성 종양 환자 31예(남서 : 여성 15 : 16, 중앙연령: 50세, 다발성골수종 14예, 비호지킨림프종 9예, 유방암 4예, 호지킨림프종 3예, 급성골수성백혈병 1예)를 대상으로 후향적 분석을 시행하였다. 결과: 채집된 단핵구의 중앙값은 5.1×10^(8)/kg (범위 1.6~189.3)이었고 CD34양성세포수의 중앙값은 12.0×10^(6)/kg (범위 1.3~75.1)이었다. 말초혈액 과립구가 500/μL 에 도달하는 중앙값은 11일(범위 8~27)이었고 혈소판이 20,000/μL에 도달하는 중앙값은 11일(범위 0~32)이었다. 고용량 항암화학요법 후 21예가 완전관해, 5예가 부분관해에 도달하였다. 생존한 환자의 중앙추적기간은 29.5개월이었고 전체 환자의 2년 무병생존기간은 55.7%이었고 중앙값은 48.2개월이었다. 2년 생존기간은 62.9%이었으며 중앙값에는 도달하지 않았다. 10예의 사망환자 중 7예가 질병의 진행으로 사망하였고, 조기사망 3예(심부전과 신부전 1예, 장파열 1예, 패혈증 1예)가 있었다. 결론: 이 연구는 포함된 환자의 수가 적고 추적기간이 짧은 단점이 있으나, 다발성골수종, 악성림프종 등의 악성 질환에서 고용량 항암화학요법과 자가조혈모세포이식은 안전하고 효과적인 치료임을 추정할 수 있었다. Background: We performed the current study to evaluate the safety and efficacy of high-dose chemotherapy (HDC) with autologous peripheral blood stem cell transplantation (ASCT) in malignant diseases. Methods: Between January 1997 and March 2003, 31 patients (14 multiple myeloma, 9 Non-Hodgkin's lymphoma, 3 Hodgkin's disease, 4 breast cancer, and 1 acute myelogenous leukemia) underwent HDC with ASCT. Stem cells were collected by CS-3000 after mobilization treatment with chemotherapy and granulocyte colony-stimulating factor. The conditioning regimens included high-dose melphalan, BEAM (BCNU, etoposide, cytosine arabinoside, melphalan), BEAC (BCNU, etoposide, cytosine arabinoside, cyclophosphamide), CPB (cyclophosphamide, cisplatin, BCNU) and others. Results: There were 15 male and 16 female patients and median age was 50 years (range: 29~77). The median number of mononuclear and CD34+ cells was 5.1×10^(8)/kg (1.6~189.3) and 12.0×10^(6)/kg (1.3~75.1), respectively. The median time of neutrophil (>500/μL) and platelet (>20,000/μL) engraftment was 11 (8~27) and 11 (0~32) days after ASCT, respectively. Twenty-two patients experienced neutropenic fever. Twenty-one patients achieved complete remission and 5 patients achieved partial remission after HDC with ASCT. The median follow-up duration for surviving patients was 29.5 months. Causes of death included disease progression (7), sepsis (1), bowel perforation (1), and renal failure (1). Median progression free survival (PFS) was 48.2 months and median overall survival (OS) was not reached. Two-year PFS and OS was 55.7% and 62.9%, respectively. Conclusion: Our results suggest that HDC with ASCT was safe and effective modality in patients with various malignant diseases.