포도상구균 폐렴을 동반한 고면역글로불린 E 증후군 1례

오흥원,서원석,신상만 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

The hyperimmunoglobulin E syndrome is a rare primary immunodeficiency disorder characterized by extremely high serum IgE levels and eosinophilia, and serious recurrent bacterial infections of the skin and sinopulmonary tract in early life. Associated clinical features are coarse facial appearance and chronic eczematoid dermatitis. The most common organism that infect these patients are S. aureus and C. albicans. The patients' serum IgE levels are elevated but the basic immunologic mechanism is not fully understood. It is believed that the elevated IgE levels reflect a T-cell imbalance characterized by T-cell activation and a deficiency of suppressor T cells to inhibit IgE production. We have experienced a case of hyperimmunoglobulin E syndrome in a ??-year-old boy who had suffered from recurrent staphylococcal pneumonia and onychomycosis and chronically pruritic dermatitis from 3 months of age with elevated serum IgE level. A brief review of the related literature is presented.

대장균 세포내 칼모듈린 돌연변이체 고발현을 위한 클로닝 및 Chemiluminescence 검출법에 의한 발현분석

오석홍,박윤식,양문식,최동성 우석대학교 자연과학연구소 1996 自然科學硏究論文集 Vol.1 No.-

To obtain a large amount of VU-4 calmodulin (115, Lys to Ile). VU-4 calmodulin gene with Ptac promoter (680 bp) was cloned into a plasmid, pUC18. Newly cloned pUC18/VU-4 was transferred into E. coli UT481. The plasmid isolated from cell extracts of the transformed E. coli was analyzed by restriction mapping and yielded the expected restriction endonuclease map with BamHI and EcoRI. Calmodulin was detected by Western blot analysis using chemiluminescence protocol and the estimated expression level was 60 pmole calmodulin/mg protein. Calmodulin was purified from the E. coli cell extracts by Ca^(2+)-dependent chromatography on a phenyl-Sepharose column. The purified calmodulin was identified as a mutant VU-4 calmodulin by SDS-PAGE and methyltransferase substrate test.

성균관대학교 물리학과 방음실의 설계 및 제작

朴興秀,尹錫旺,李聖壽,朴光俊,吳世珍 성균관대학교 기초과학연구소 1986 論文集 Vol.37 No.1

An anechoic chamber was constructed to make to condition of acoustic free field at the Department of Physics, Sung Kyun Kwan University. In this paper the criteria for the design and construction of the anechoic chamber are described. 48cm long wedges of density 15.3kg/㎥ glasswool attached to the 40×20×20㎤ base were used for the lining of anechoic chamber with the cutoff frequency of 125Hz. The interior space of the chamber is 3.56m long, 3.38m wide and 2.37m high.

치아미백제의 미백효과에 관한 연구

이용근,이흥수,이성재,방기숙,오상엽 원광대학교 생체재료·매식연구소 1996 원광생체재료·매식 Vol.5 No.2

Human teeth vary widely in color. Practitioner and patients are concerned with preventing and correcting discolored or dark teeth to achieve and maintain stain-free, white teeth. Tooth brushing cannot after tooth color but it can remove adherent films and stains. Esthetics of natural dentition can be improved by bleaching and this process can be applied to intrinsically and extrinsically stained teeth. The need for a brighter, more attractive smile has made rapid growth in the marker for tooth whiteners. There is no doubt these products work as whiteners, at least on mild to moderate stains, but the safety of these products are unclear. In this experiment, the effect of tooth whitener application on the color of extracted human enamel was measured. RMS, RMT and NWT were used as tooth whiteners, and tooth paste(ETQ) and hydrogen peroxide solution(HPO) were used as controls. 35 caries-free extracted human molars were embedded and polished with the exposed enamel diameter of 4 mm. The tooth whiteners and control agents were according applied to the manufacturers' instructions of clinically simulated procedures for eight weeks, and measurements were repeated every two weeks. CLELAB color difference(ΔE^*) was measured using Differential Colorimeter(Model TC-6FX, Denshoku Co., Japan). The results were are follows ; 1. After application of tooth whiteners and controls for eight weeks, the color change of all groups were not significantly different from each other (p>0.01). But after application of agents for 2 weeks and four weeks, the color difference of NWT applied group and HPO applied group were significantly higher than those of others(p<0.01). 2. The application of tooth paste and paste type tooth whitener made gradual color change, but hydrogen peroxide gel type whitener and hydrogen peroxide solution made rapid color change during initial application period.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1994)

김재홍,안진균,정성재,김영태,김중환,김시영,이석종,이홍렬,서호석,김경훈,권혁진,정우권,고우석,이용석,안필수,오준규,오용섭 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

Background : In recent years gonorrhoea has been panendemic and remains one of the most common Sexually Transmitted Diseases in the world, especially in developing countries. Objective & Method : For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria Gonorrhoeae(PPNG), Ive have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1994, 168 strains of N. gonorrhoeae were isolated, among which 109 (64,9% ) were PPNG. Conclustion : Our results suggests that the prevalence of PPNG in Seoul is still increasing.

Purification and Characterization of Phytoferritin

Oh, Suk-Heung,Cho, Sung-Woo,Kwon, Tae-Ho,Yang, Moon-Sik Korean Society for Biochemistry and Molecular Biol 1996 Journal of biochemistry and molecular biology Vol.29 No.6

Ferritins from germinated pumpkin seeds were isolated by ammonium sulfate precipitation (0.55 saturation), ion-exchange chromatography on DEAE-cellulose, and gel filtration chromatographies on Sephacryl S-300 and Sephadex G-100. Pumpkin ferritin contains less iron than soybean ferritin. Pumpkin ferritin cross-reacted with anti-soybean ferritin antiserum made in rabbit, and showed two distinct antibody reactive bands, both of equal intensity. The pumpkin ferritins corresponding to the two bands were separable by centrifugation in a sucrose gradient (20~50%). The molecular weights of the native pumpkin ferritins based on the estimation of sucrose gradient centrifugation, gel filtration on Sephacryl S-300 and non-denaturing polyacrylamide gel electrophoresis appeared to be: 530~580 KD (the large molecular weight pumpkin ferritin) and 330-360 KD (the small molecular weight pumpkin ferritin) The large molecular weight pumpkin ferritin contains less iron. Both pumpkin ferritins cross-reacted with anti-soybean ferritin antibody with a spur formation suggesting partial antigenic recognition.

Optimization of Kimchi Function and Anti-Obesity/Anti-Inflammatory Effect

Suk-Heung Oh 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Kimchi has been selected as one of the world’s top five health foods, including vitamins A, B, and C, including rich lactic acid bacteria. The kimchi is thought to be different in taste and nutrition, bacterial community, transcriptional body, protein and metabolite according to the material, manufacturing method, fermentation method, and fermentation time. Special amino acids found in kimchi include GABA and Ornithine, and these special amino acids are variously distributed according to the fermentation process of kimchi and the role of lactic acid bacteria. These amino acids are used as various types of health functional foods and cosmetics materials at home and abroad, where their functionality is known first in terms of medicine. The results of using Weissella koreensis OK 1-6 lactic acid bacteria with excellent ornithine production capacity in kimchi showed that the number of lactic acid bacteria and the content of ornithine in kimchi were significantly increased. In the obesity state induced by high fat intake, the weight of experimental animals decreased significantly in the diet group with normal kimchi and Weissella koreensis OK 1-6 kimchi, and decreased even more in the Weissella koreensis OK 1-6 kimchi addition group. The weight of epididymal fat and blood leptin level were significantly decreased in Weissella koreensis OK 1-6 kimchi group. The concentration of triglyceride and blood leptin in liver tissues were significantly lower in Weissella koreensis OK 1-6 kimchi group than in high fat group or general kimchi group. In the liver tissue of Weissella koreensis OK 1-6 kimchi group, the expression of PPAR, ACC, LXR, C/EBP, and SREBP2 mRNAs involved in lipid synthesis was low and the expression of CPT-1 mRNA involved in fatty oxidation was high. Anti-inflammatory effects of kimchi and Leuconostoc mesenteroides DRC 1506, kimchi lactic acid bacteria and the protective effects of intestinal mucosal function were investigated when colitis was induced by dextran sodium sulfate (DSS). As a result, kimchi and Leuconostoc mesenteroides DRC 1506 inhibit the growth of Enterobacteriaceae, inhibiting the expression of NF-κB gene, inhibiting the expression of inducible enzymes such as proinflammatory cytokines (TNF-α, IL-6, and IL-1β) and COX-2 or iNOS, and increasing anti-inflammatory cytokines (IL-10) production. In addition, the reduction of inflammatory response and the decrease of intestinal harmful bacteria increased the expression of tight junction protein and mucin-producing genes, which maintain the intestinal defense membrane, thereby preventing damage to the intestinal barrier integrity. (This study was supported in part by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, and in part by a research grant from Daesang Co. to S.H. Oh, Woosuk Univ. Korea.)

Carnitine and Calmodulin N - Methylation in Rat Testis : Calmodulin May be Involved in Carnitine Biosynthesis

Suk-Heung Oh,Youn-Soo Cha,Hee-Sook Sohn 한국식품영양과학회 1998 Preventive Nutrition and Food Science Vol.3 No.3

Rat testis known to contain all of the enzymes required for carnitine biosynthesis also contains high concentrations of calmodulin, a protein which mayor may not contain trimethyllysine, the major substrate in carnitine biosynthesis. The purpose of this study was to investigate the levels of carnitine and the state of calmodulin N-methylation in rat testes, and to discuss the possibility of the involvement of calmodulin in carnitine biosynthesis. Nonesterified carnitine, acid soluble acyl carnitine, and acid insoluble acyl carnitine of rat testes were 273 nmole, 62 nmole, and 4 nmole/g tissue, respectively. Total carnitine level was 339 nmole/g testes tissue. Calmodulin purified from rat testes was assayed for methylation potential using N-methyltransferase from the rat testes. Rat testes calmodulin showed no ³H-methyl incorporation indicating that the calmodulin was trimethylated already by endogenous calmodulin N-methyltransferase. Amino acid composition analysis revealed that the rat testes calmodulin contained one mole of trimethyllysine per mole of calmodulin. These data suggest that testes calmodulin could provide the trimethyllysine needed for the synthesis of carnitine in the rat testes.

Effect of Diets Supplemented with Pharbitis Seed Powder on Serum and Hepatic Lipid Levels, and Enzyme Activities of Rats Administered with Ethanol Chronically

Oh, Suk-Heung,Cha, Youn-Soo Korean Society for Biochemistry and Molecular Biol 2001 Journal of biochemistry and molecular biology Vol.34 No.2

The levels of $\gamma$-aminobutyric acid (GAGA) have been analyzed from pharbitis seeds by an AccQ-Tag amino acid analysis procedure. The GABA level of the pharbitis seeds was 125 nmole per gram fresh weight. To investigate the effects of pharbitis seed diets on serum and hepatic lipid levels, as well as enzyme activities of rats administered with ethanol chronically, Sprague-Dawley male rats were fed with either a AIN-76 diet (control), a control diet plus ethanol, a control plus pharbitis seed diet, or a control plus pharbitis seed diet plus ethanol for 30 days. Pharbitis seed diets decreased the serum total cholesterol, triglyceride, LDL-cholesterol, and $\gamma$-GTP levels that were increased by the chronic ethanol administration. In addition, pharbitis seed diets decreased the liver triglyceride and total lipid levels that were increased by the ethanol administration. However, ethanol metabolism was not retarded by the pharbitis seed supplemented diets. The present Endings, plus previous data showing the differences in the effects of cabbage diets having a high or a low level of GABA on the lipid levels and the enzyme activities of rats (Cha and Oh [2000] J. Korean Soc. Food Sci. Nutr. 29, 500-505), raise the possibility that GABA in plants could have a nutraceutical role in the recovery of chronic alcohol-related diseases.

Cloning and Characterization of a Rice cDNA Encoding Glutamate Decarboxylase

Oh, Suk-Heung,Choi, Won-Gyu,Lee, In-Tae,Yun, Song-Joong Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.5

In this study, we have isolated a rice (Oryza sativa L.) glutamate decarboxylase (RicGAD) clone from a root cDNA library, using a partial Arabidopsis thaliana GAD gene as a probe. The rice root cDNA library was constructed with mRNA, which had been derived from the roots of rice seedlings subjected to phosphorus deprivation. Nucleotide sequence analysis indicated that the RicGAD clone was 1,712 bp long, and harbors a complete open reading frame of 505 amino acids. The 505 amino acid sequence deduced from this RicGAD clone exhibited 67.7% and 61.9% identity with OsGAD1 (AB056060) and OsGAD2 (AB056061) in the database, respectively. The 505 amino acid sequence also exhibited 62.9, 64.1, and 64.2% identity to Arabidopsis GAD (U9937), Nicotiana tabacum GAD (AF020425), and Petunia hybrida GAD (L16797), respectively. The RicGAD was found to possess a highly conserved tryptophan residue, but lacks the lysine cluster at the C-proximal position, as well as other stretches of positively charged residues. The GAD sequence was expressed heterologously using the high copy number plasmid, pVUCH. Our activation analysis revealed that the maximal activation of the RicGAD occurred in the presence of both $Ca^{2+}$ and calmodulin. The GAD-encoded 56~58 kDa protein was identified via Western blot analysis, using an anti-GAD monoclonal antibody. The results of our RT-PCR analyses revealed that RicGAD is expressed predominantly in rice roots obtained from rice seedlings grown under phosphorus deprivation conditions, and in non-germinated brown rice, which is known to have a limited phosphorus bioavailability. These results indicate that RicGAD is a $Ca^{2+}$/calmodulin-dependent enzyme, and that RicGAD is expressed primarily under phosphate deprivation conditions.