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      • Oil Contents and Fatty Acid Composition of Korean Perilla (Perilla ocimoides L.) Collections

        Hong,Seong-Taek,Son,Suk-Yeong,Jong,Seung-keun,Rho,Chang-Woo,Yun,Jong-Sun 한국자원식물학회 2003 Plant Resources Vol.6 No.3

        This study was carried out to obtain informations on the variations of oil content and fatty acid composition among 90 Korean perilla collections. Average oil content of 90 perilla collections was 44.2% with a range from 29.7% to 61.9%. Perilla collections with late-maturing, super-large seed and gray seed coat showed higher oil content than other types in general. Average saturated fatty acid content in perilla oil was 9.0% with a range from 8.2% to 10.7%, while average unsaturated fatty acid content varied from 89.3% to 91.8% with a mean of 91.0%. Contents of saturated and unsaturated fatty acids were not related to maturity. There were no differences in the contents of saturated and unsaturated fatty acids according to maturity. Linoleic acid and linolenic acid contents were the highest in the super large-sized group(15.5%) and in the large-sized seed group(61.4%), respectively, while contents of fatty acids among the perilla collections were variable with different seed coat colors. Most of the traits studied were not significantly correlated with oil content, but linoleic acid($\omega$ 수식 이미지-6) content was negatively correlated (r=-0.217*) with linolenic acid(ω-3) content.

      • KCI등재
      • 김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

        조영배,최현정,백형석,전홍기 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

        김치 발효 숙성기간을 연장하고 신선도를 오랫동안 유지 할 수 있는 김치발효 starter의 개발을 목적으로 최적숙성기에 있는 김치로부터 bacteriocin생성능이 없는 유산균을 분리하여 동정하였으며, bacteriocin생성능이 우수한 L.acidophilus 88을 융합시키기 위해 electrofusion에 대한 최적 조건을 검토하였다. 분리균주는 Lactobacillus속으로 동정되어 Lactobacillus sp. JC-7이라 명명하였다. Electrofusion에 의해 생성된 융합주를 식별하기 위해 streptomycin(2.5㎎/ml)에 내성을 나타내는 Lactobacillus sp. JC-7변이주와 kanamycin(600㎍/ml)에 내성을 나타내는 L.acidophilus 88변이주를 분리하였다. Electrofusion을 100V/㎝, 120msec(72ohms,1670 capacitance) 에서 수행했을 때 융합효율이 가장 양호하였으며 전기장의 세기와 시간이 중가할수록 융합 효율이 현저히 감소하였다. 2가 양이온은 농도가 중가할수록 대체적으로 융합효율을 감소시키는 경향을 나타내었으나 1mM MGCl_2에서는 대조군에 비해 융합효율이 약간 증가하였다. PEG매개에 의한 융합법의 융합효율을 비교한 결과, 융합효율은 chemical fusion<electrofusion<electrofusion+20% PEG순을 나타내었다. Evaluation of Optimum Conditions for the Electrofusion between Lactobacillus sp. JC-7 Isolated from Kimchi and Lactobacillus acidophilus 88. Young-Bae Jo, Hyun-Jung Choi, Hyung-Suk Baik and Hong-Ki Jun*. Department of Microbiology, Pusan National University, Pusan 609-735,Korea-A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus thrugh its morphological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin(2.5㎎/ml)resistant mutant of Lactobacillus acidophilus 88 and kanamycin(600㎍/ml) resistant mutant Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120msec(1670㎌), a field strength of 100V/㎝,and a pulse controller setting of 72Ω. The potimum pH of elecroporation buffer was 7.5 and the concentration of divalent cation was 1mM MG^2+. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to electroporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

      • KCI등재후보

        생약추출물이 초기우식법랑질의 재광화에 미치는 효과

        홍석진,손재범,박근형 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.2

        Since plant extracts have shown antibacterial activities, we hypothesized that herbal plant extracts would further increase cariostatic efficacies of the fluoride dentifrices.OBJECTIVE: The purposes of this study were to determine the minimum inhibitory concentration(MIC) of 9 different Korean herbal plants extracts on the S.sobrinus KCTC3308 and the efficacy of NaF-Silica dentifrices with or without the extracts. METHODS; The MIC of the methanol-extracts on the growth of S.sobrinus was determined. Subsurface caries lesions were prepared as previously described (Hong et al.J Dent Res. 77:843, 1998). After Vickers hardness number(VHN) were determined, the specimens were assigned to 9 test groups of 12 each by randornized blocks with VHN stratification. The specimens were tested for remineralization In vitro subsurface enamel lesion under cyclic microbial dynamic condition simulating in vivo carious fomation. The statistical significance of delta VHNs was determined by Students t-test and one-way ANOVA. RESULTS: The MIC of the Angelica gigas extract was 3 ㎎/㎖, as same as Akebia quinata and Camellia sinensis was 4 ㎎/㎖. However, all 9 extracts tested in the study demonstrated the synergistic cariostatic potential with known fluoride dentifrices. CONCLUSION: The NaF-herbal extract in silica base dentifrice significantly increased the cariostatic effect of NaF-Silica base dentifrice.

      • 정상 성인에서 Amitriptyline이 수면구조와 정신생리적 기능에 미치는 영향

        홍성곤,신석철,왕성근 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

        To investigate the effects of Amitriptyline on sleep structure and psychophysiologic function in normal adults, author applied polysomnography for sleep structure, reaction time for attention and visual analogue test for daytime fatigue, mood and sleepiness to the volunteers. The subjects were 8 healthy young adults, aged from 20 to 24 years old. The results were summarized as follows. 1. In normal adults, there were great individual variations in the ditribution of relative value(%) of sleep structure. 2. Compared with the baseline, total sleep time and sleep efficiency increased significantly in the 2lmg amitriptyline night, and WASO decreased significantly in the second recovery night. 3. Compared with the baseline, the absolute value(minutes) of stage 1 sleep decreased significantly in the amitriptyline, first and second recovery nights, and the relative value(%) of stage 1 sleep decreased significantly in the first recovery night. The absolute and relative value of stage 2 sleep increased significantly in the amitriptyline night. The relative value of slow wave sleep decreased significantly in the first recovery night. 4. Compared with the baseline, the absolute and relative value of REM sleep decreased significantly in the amitriptyline night, the relative value of REM sleep increased significantly in the first and second recovery nights, and REM latency shortened significantly in the first and second recovery nights. 5. Feeling of fatigue, depressed mood and sleepiness increased in the afternoon the day after amitriptyline administration. 6. 1) In the baseline, daytime sleepiness was positive correlation with feeling of fatigue and mood. 2) The day after amitriptyline administration, relative value of REM sleep was negative correlation with feeling of fatigue and sleepiness, daytime fatigue was positive corelation with mood and sleepiness, and daytime mood was positive correlation with sleepiness. 3) In the first recovery day, daytime fatigue was positive correlation with mood and sleepiness, and negative correlation with reaction time, daytime mood was positive correlation with sleepiness, and reaction time was negative correlation with sleepiness. 4) In the second recovery day, total sleep time was positive correlation with the relative value of REM sleep time, the relative value of stage 2 sleep was negative correlation with the relative value of slow wave sleep, the relative value of slow wave sleep was positive correlation with daytime fatigue and mood, daytime fatigue was positive correlation with mood and sleepiness, and negative correlation with reaction time, daytime mood was positive correlation with sleepiness, and reaction time was negative correlation with sleepiness.

      • 數學의 公理的 理論에 있어서 眞理의 問題

        洪錫浩 건국대학교 교육대학원 1981 敎育論叢 Vol.1 No.-

        수학적 대상의 추상적 성격과 이론구성의 공리적 방법은, 전체적으로 과학은 진리의 문제해결이라는 큰 테두리에서 보아야 하며, 폭을 좁혀 실질적 내용을 수반하는 추상적 대상에 관해서는 수학적 개념에 포함된 자연과학의 이론에 의존하여야 된다. 소수의 정의와 공리로부터 정리가 논리적으로 유도되는 수학이론의 공리적 구성에 있어서는, 그 이론의 진리성의 문제는 공리 및증명에 적용되는 논리적 추론방법의 진리성의 문제에 귀착된다. 순수수학에서 증명의 엄밀성은 정리를 수학적 진리로 인정하기 위해서 큰 의의를 갖는다. 수학자는 누구나 정리가 공리로부터 엄밀히 논리적으로 유도되면 그 정리는 증명되었다고 생각한다. 수학에 잇어서 추론의 엄밀성의 기준을 얻으려면, 논리적 연관의 해석을 위해, 형식의 정확한 언어를 적용하는 수학적 논리학에 관해서 연구되어야 한다. 언어의 표현에서 생길수 있는 불명료한 것들은 언어의 힘으로 제거될 수 있다. 학문의 현상에 의해서 생길 수 있는 수학적 진리에 관련되는 몇 가지 문제는 순 수학적 방법으로 해결가능하다. 그러나, 보다 상세히 분석해 보면 증명의 엄밀성이란, 개념 그 자체와 마찬가지로, 본질적으로는 추론의 이론적 원칙과 방법등의 진리성에 있어서 특히 철학적 분석을 필요로 하고 있다.

      • 전압제어형 DC/DC Converter의 평균 모델을 위한 Duty Cycle 모델

        홍성수,사공석진,안현식 국민대학교 생산기술연구소 2002 공학기술논문집 Vol.25 No.-

        The conventional average model for a pulse width modulator employed in a voltage-mode-controlled pulse width modulated converter tends to be numerically unstable when the on-time duty ratio becomes sufficiently small. This paper presents a new average model for a voltage-mode control modulator that is not susceptible to such numerical problems. The validity of the proposed model is confirmed with cycle-by-cycle simulations using an exact discrete-time model.

      • KCI등재후보

        들깨 유전자원의 단백질함량과 지방 및 지방산 특성

        홍성택,손석용,노창우,이경희,정재현,박재성 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.4

        공시된 들깨 지방재래종 90개 품종의 단백질함량, 지방 및 지방산을 분석한 결과 다음과 같다. 1. 숙기별로는 중ㆍ만생종의 평균단백질 함량은 22.4%로 비교적 낮았고, 품종분포가 대부분 평균치에 가까웠으며, 조생종은 평균단백질 함량이 24.1%로 비교적 높았다. 2. 평균단백질함량은 소립종(천립중3.0~4.0g이하)이 23.0%로, 중립종(천립중 4.0~5.0g)이나 대립종(천립중 5.0~6.0g)의 22.1%보다 높은 경향이었다. 3. 기름함량은 조생종이 42.6%로 가장 적었고, 중생종이 44.5%, 만생종이 45.5%로 기름함량이 많았으며, 립중별로는 소립종이 44.2%, 중립종이 43.1%, 대립종이 41.5% 대립종일수록 낮은 경향이었다. 4. 지방산조성은 숙기가 늦어짐에 따라 Oleic acid와 Linolenic acid는 다소 많았고, Linoleic acid는 적어지는 경향이었다. Linoleic acid는 극대립종에서 15.5%로 가장 높았고 립중이 가벼울수록 높아지는 경향이었다. 5. 기름함량과 지방산 비율간의 상관 정도를 보면 기름함량과 Palmitic acid, Linoleic acid는 負의 상관이나, Stearic acid, Linolenic acid는 正의 상관을 보였다. Stearic acid는 Oleic acid와 유의적인 정의 상관을 , Linoleic acid와는 부의 상관을 보였다. Oleic acid는 Linoleic acid 및 Linolenic acid와 고도로 유의한 부의 상관을, Linoleic acid는 Linolenic acid와, 포화지방산은 불포화지방산과 유의한 부의 상관을 보였다. Korean local collected strains or varieties of perilla analyzed on their protein and oil content and fatty acids composition in the seed. The strains analyzed were 90 of which grown in the experimental field of Chungbuk Agricultural Research and Extension Services at Cheongwon and Umseong from 2001 to 2002. The seed protein contents of 90 perilla varieties ranged from 19.1% to 29.0% and the average content was 22.8%. There differed by the seed coat color showing 1.9% higher content in light gray than that of dark brown, which may suggest that the seed coat color could be used as a marker gene for breeding high protein varieties. Oil content of medium and late varieties in maturity showed higher as compared to early varieties, and appeared differences by seed coat color, and small seed varieties showed higher seed oil content than in large seed varieties by seed size. Linoleic and linolenic acid content of which major fatty acids in perilla seed were 74.4% in average of tested varieties. Fatty acid composition of perilla varieties were not significantly different by maturity, seed coat color and 1,000 seed weight. Oil content showed high positive correlation, with stearic and linolenic acids and negative correlation with linoleic acid.

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