Interacelluar reprogramming of expression, glycosylation, and function of plant-derived anti-virus therapeutic monoclonal antibody

Da-Young Park,Kyung-Jin Lee,Yangkang So,Zhe Lu,Seulki Lee,Jung-Hwan Lee,Seung Han Oh,Yeon Soo Han,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2010 한국당과학회 학술대회 Vol.2010 No.1

Plant genetic engineering has led to production of plant-derived mAb (mAbP), which can provides a safe and economically effective alternative to the conventional antibody expression methods. In this study, the expression levels of mAbP SO57 with or without ER-retention peptide extensions signal (Lys-Asp-Glu-Leu; KDEL) in transgenic tobacco plants were analysed in transgenic plant. The expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL regardless of transcription level. mAbP SO57 with KDEL localized surround to the nucleus suggesting that the mAbP with KDEL is localized in ER. The mAbP without KDEL and mAbH had mainly Golgi type glycans, whereas the ER-localized mAbP with KDEL had glycan profile with both oligomannose type (47.6%) and Golgi type (52.4%). The Fc domains of both purified mAbP (with and without KDEL) and human-derived mAb (mAbH) had similar bingding activity to the Fcγ RI receptor (CD64). Both mAbP (with and without KDEL) had a shorter half-life than mAbH. However, both mAbP with and without KDEL was as effective at neutralizing activity of the rabies virus CVS-11 as the mAbH. These results suggest that ER localization of recombinant mAbP by KDEL reprograms glycosylation and enhances production of the functional antivirus therapeutic antibody in the plant.

한국 재래산양에서 Bovine Viral Diarrhea, Bovine Herpesvirus-1, Parainfluenza Type-3 바이러스에 대한 혈청학적 연구

한동운,권용국,문진산,윤소라,위성환,장환,탁동섭,이태욱,강문일 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.3

The 948 goat sera were serologically tested for ten pathogens including bovine viral diarrhea (BVD), bovine herpes virus type-1 (BHV-1) and parainfluenza virus type-3 (PI-3). By viral neutralizaion test, three pathogens including BVD virus, BHV-1, PI-3 were applied for screening each neutralizing antibody and its titration. Among 948 sera, there were 215 seropositives (22.7%) against BVD virus, 25 (2.6%) against BHV-1 virus, and 161 (17.0%) against PI-3 virus, respectively. In the seropositive cases to BVD virus, provincial prevalence of them were recorded as Gyeongnam(30.8%) for the highest one, while as Jeonbuk (15.8%) and Gyeongbuk(l6.7%) relatively for the lower ones. Among them, higher than 1:8 in antibody titration was classified as 74 cases (34.4%) which were suspected the cycle of its infection in their herds. Among the seropositives to BHV-1, it was relatively lower prevalence rate ranging from 1.7% to 6.7% that those of BVD virus and PI-3, but more importantly they were prevailing across the country. Most of the seropositive cases (42.9%) to PI-3 were classified as 1:4-1:16 in titration, but also distributed all around the country. The prevalence in goats varied according to areas (15.8 to 30.8%). Related to the provincial prevalence of three viral agents in Korean goats, it seemed that three viral pathogens would be directly or indirectly involving in various disorders as primary or secondary causative agents resulting in decreased productivity.

‘병동 중심의 처치수가 산정지침 개발’ 등을 통한 간호업무 표준화 및 그 효과

한혜정,설미진,김영주,박소영,박아리스,문한경,이현영 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.1

문제: 병동 처치수가 산정 부정확 및 누락으로 인한 부적절한 재고관리로 진료차질, 불필요한 업무발생 및 수익이 감소한다. 목적: 병동 처치수가 산정 정확화를 위한 업무 표준화 도구 및 시스템을 개발하여 적정 재고관리를 통한 수익증대 및 직무만족도를 향상시킨다. 의료기관: 서울시 종로구에 소재한 대학병원 질 향상 활동: 병동중심의 처치 산정지침 개발 및 수가물품의 적정재고 관리방안을 모색하였다. 개선효과: 병동중심의 처치수가 산정 지침서를 제작하여 업무표준화를 기하였음. 응급청구 품목 및 수량이 ’07년 대비 71%감소하고 타 병동 차용품목이 활동 전에 비해 61% 감소, 수량은 77% 감소함. 응급청구 총소요시간이 활동 전에 비해 ’07년 대비 77% 감소하고 타 병동 차용 총 소요시간이 61% 감소함. 전년 동기간 대비 수익이 4% 증가하였으며 또한 54병동과 보험 심사팀에서 직원들의 직무만족도가 향상되었다.

한국 재래 산양에서 Leptospira spp.에 대한 혈청역학적 연구

한동운,권용국,문진산,윤소라,위성환,장환,탁동섭,이태욱,강문일 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.3

Between January 1999 and September 2001, 948 sera (120 from each province except 108 from Chungbuk) from 8 provinces were sampled from Korean goats. All the samples were serologically tested for Leptospira spp. By the microscopic agglutination test against four L. interrogans serovars including canicola, icterohaemorrhagiae, pomona and hardjo, 948 Korean goats were confirmed as 11 seropositives (1.2%) only infected with L. icterohemorrhagiae. All positive sera were collected from Gyeonggi (5), Gangwon (4) and Chungbuk (2). Titration of them were classified as 1:100 of 10 sera except one case with 1:200 from Gyeonggi. It was meaningful that the pathogen in Korean goats was mainly distributed in the northen part of the country. Consequently, the results in the study were confirmed and analyzed the recent status of infection of leptospirosisas zoonotic agentsome in Korean goats.

숙련도에 따른 배드민턴 스매쉬 동작의 운동학적 변인 비교

소재무,한상민,서진희 한국운동역학회 2003 한국운동역학회지 Vol.13 No.2

So, J-M. Han, S-M. S대, J-h. Comparison of the Kinematic Variables int he Badminton Smash Motion. Korean Journal of Sport Biomechanics, Vol. 13, No. 2, pp. 65-74. The purpose of this study was to analyze Kinematic variables in the badminton smash motion through 3-dimensional image analysis. The kinematic variables were velocity of joints in upper limbs, the angle of wrist in the impact, and the angular velocity of the top of racket head. The smash motions of four male badminton players in H University and four male students at department of the physical education in K University who were not majoring in badminton were analyzed kinematically and the attained conclusions were as follow. 1. The velocity of segments in upper limbs of the unskilled group was faster than that of the skilled group. The movement pattern was fast back swing-slow impact moment-fast follow through in the unskilled group, but slow back swing-fast impact moment-slow follow through in the skilled group. 2. As the BS phases, the velocity of segment in right shoulder was different significantly between groups. Right elbow and right wrist segments, velocity of racket head was different significantly between groups(p<.05) by IP phases. As the FT phases, there was no significant difference. 3. The angle of right wrist at the impact, the angle of dorsi flexion and the angle of palm flexion in aspect were shown that the skilled group was higher that unskilled group. There was no significant difference. 4. The velocity of racket head was shown that the unskilled group has fast velocity, but the angle velocity was shown the unskilled group has slow. 5. The angle velocity of racket head in aspect were no significant difference between groups, but maximal angle velocity was different significantly between groups(p<.05).

항우울제 장기 투여에 의한 흰쥐 해마에서 PhosphoCREB의 발현 증가

김소양,한진희,이창욱,백인호 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

목 적 : 저자들은 paroxetine(selective serotonin reuptake inhibitor). desipramine(selective norepinephrine reuptake inhibitor), moclobemide(reversible monoamine oxidase inhibitor)의 항우울제를 흰쥐에 장기 투여한 후 해마에서 phosphoCREB의 발현정도를 측정하고 이를 항정신병약물, 항불안제 및 control을 장기 투여하였을 때의 발현정도와 비교해 보고자 하였다. 방 법 : 실험동물로는 체중 200∼300g의 Sprague-Dawley 수컷 흰쥐 240마리를 사용하였고, paroxetine 투여군, desipramine 투여군, moclobemide 투여군, haloperidol 투여군, lorazepam 투여군과 대조군으로 나누고 각각의 약물을 1일간, 3일간, 7일간, 14일간 각각 하루 한차례씩 복강내 주입을 하였다. 흰쥐는 마지막 투약 15분후에 대뇌를 적출하였고 면역조직화학법을 이용하여 해마에서 발현되는 phosphoCREB(+)신경원 수를 측정하였다. 결 과 : 항우울제인moclobemide 투여군에서 3일째부터 phosphoCREB의 발현을 증가시켜 7일째와 14일째 모두 증가되었고 paroxetine 투여군과 desipramine 투여군도 7일째와 14일째 증가를 나타내었다. 그러나 항우울제가 아닌 약물을 투여한 군(lorazepam 투여군과 haloperidol 투여군)에서는 대조군과 비교하였을 때 투여 기간에 따른 phosphoCREB의 증가는 없었다. 결 론 : 다양한 항우울제의 장기 투여는 항정신병약물과 항불안제의 장기 투여와 비교하면 모두 해마에서 phosphoCREB의 발현을 증가시킴을 알 수 있었다. 따라서 이는 다른 약제와 구별되는 항우울제만의 특이한 공통적 약물작용기전으로 생각된다. Objectives : This study was designed to assess the expression of phosphoCREB in rat hippocampus after chronic administration of various antidepressants in comparison with chronic administration of antipsychotic and antianxiety drugs. Methods : Male Sprague-Dawley rats(200-300g) were used for this experiment. The subjects were divided into 6 groups according to specific treatment agents(paroxetine, desipramine, moclobemide, haloperidol, lorazepam, vehicle) which were administered daily for 1day, 3day, 7day, and 14days by intraperitoneal injection respectively. Brains were removed 15 minutes after the last treatment. PhosphoCREB immunoreactivity was measured by phosphoCREB(+) cell counts in hippocampus of rats. Results : Expression of phosphoCREB was significantly increased from day 3 in moclobemide group, from day 7 in paroxetine and desipramine groups, and increased most significantly from day 14 in all antidepressant-administered groups, with no increase in other two groups(lorazepam and haloperidol groups) throughout the experiment and even after 14 days of treatment. Conclusion : These result suggest that increased expression of phosphoCREB after chronic administration of antidepressants, not of antipsychotic or antianxiety drugs, demonstrates pharmacolgical specificity of antidepressant treatment in rat hippocampus regardless of their receptor preference.

심부전증에서 Doppler Tissue Image를 이용한 비동시성 심실수축(Myocardial Dyssynchronicity)의 측정 : 심전도와비교 Comparison with EKG

구소영,조구영,한성우,최승혁,박우정,두영철,홍경순,오동진,이영 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.4

경직성 뇌성마비 환아에서 선택적 후근 절제술 후 운동기능의 변화

이소영,최인성,한재영,정주찬,위정선,오명신,박승진,이삼규,김재휴 全南大醫大 2001 전남의대학술지 Vol.37 No.1

혈전탄성묘사도를 이용한 만성 신부전 환자의 수술 전 혈액 응고 기능 평가

김소영,이상귀,손지선,한영진,송희선 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.4

Background: Patients with chronic renal failure traditionally have been recognized as being at risk for perioperative bleeding diathesis. However, there has been a few reports that chronic renal failure patients showed a hypercoagulability. The purpose of this study was to assess blood coagulation profiles in patients with chronic renal failure using a thromboelastography. Methods: Thirty patients (ASA physical status, 2 and 3) with chronic renal failure (experimental group), were scheduled to get presenting for an arteriovenous fistula formation, and 30 patients with normal renal function (control group) were randomly selected. Blood sampling for a thromboelastography was performed prior to induction of anesthesia. A thromboelastography was analyzed by measuring R time, K time, maximum amplitude (MA), α degree, and TEG index. Laboratory tests including serum blood urea nitrogen, creatinine, hematologic data (hemoglobin, hematocrit, platelet count), and coagulation data (PT, PT-INR, aPTT, BT) were also measured in all patients preoperatively. Results: In the thromboelastographic indices, K time was significantly decreased and MA, α angle, and TEG index were significantly increased in patients with chronic renal failure (experimental group) compared with the control group (P < 0.05). However, there were no significant differences of PT, PT-INR, aPTT, BT between the groups. Conclusions: Thromboelastographic data demonstrates a hypercoagulable state in patients with chronic renal failure. This finding suggests that traditional concern for bleeding diathesis in patients with chronic renal failure may require reassessment. (Korean J Anesthesiol 2002; 43: 407~412)

시ᆞ도 건강가정지원센터의 운영모형 개발 연구 : 역할 및 조직을 중심으로 It's Roles and Organization

장진경,오제은,한은주,류진아,원소연 한국가족관계학회 2006 한국가족관계학회지 Vol.11 No.1