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토끼의 응집소생산과 혈청살균작용에 미치는 Chloramphenicol과 Cortisone의 영향
金在烋 中央醫學社 1970 中央醫學 Vol.19 No.3
Recently many works have been reported concerning the effect of chloramphenicol on the protein synthesis. The findings, however, are not always agreeable each other; some asserting that the synthesis of protein is inhibited by the presence of chloramphenicol while others reporting the opposite role of the antibiotics. Cortisone is a hormone also known as an agent having complicated influences on the protein synthesis. Employing rabbits as experimental animals and Salmonella typhi H901 as a test organism, anexperiment was undertaken to see whether chloramphenicol and cortisone have any effect on the antibody formation and the bactericidal action of the immune sera with the following results. 1. Primary, secondary, and tertiary immune responses to the heat-killed Salmonella antigens of the rabbits treated with chloramphenicol or cortisone were not significantly different each other or from the non treated control. Dosages of chloramphenicol and cortisone in each injection were 100mg/kg or 200 mg/kg and 10 mg/kg or 20 mgikg, respectively. Number of injections were from once to 15 for chloramphenicol and from twice to 15 for cortisone. The first injection was done 10 min. before first antigen inoculation and the remaining at 12 hour intervals following the first in both cases. 2. In spite of the indistinct agglutination titer between immune sera, the bactericidal action of the sera showed a marked difference between the chloramphenicol and cortisone-treated rabbits serum and the non-treated control. The heavier dosages of chloramphenicol or cortisone were given, the lower was the bactericidal action of sera. 3. When serum was diluted with sterile saline, the difference of bactericidal activities between the sera disappeared gradually following the degree of dilution, finally reaching the level of saline solution. 4. The author has tried to find a clue in the alternation of complement components or in.
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김재휴 中央醫學社 1970 中央醫學 Vol.19 No.4
D-mannose solution (1%)has been reported as being moderately bactericidal to Gram-negative organisms. A comparative experiment was undertaken to see whether this solution has any effect on the serum bactericidal activity when used as serum diluent and cell-suspending solution 1. Immune serum against Shigella flexneri la showed a considerably reduced bactericidal action as compared with normal one regardless of the kind of medium used in the bacterial suspension and serum dilution, but in the neighbourhood of 1 : 100 dilution normal serum showed more survived cells than immune serum. 2. At comparatively low dilutions, serum factors concerned with bactericidal activity exhibited their functions better in saline than in mannose solution, but at highly diluted serum the bactericidal activity seemed solely controlled by the medium, i.e., in mannose-containing test tubes the survival rate of cells was far less than in saline-containing ones.
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