LPS로 유도된 RAW 264.7세포에 대한 벼메뚜기(Oxya chinensis sinuosa) 에탄올 추출물의 항염증 효과

윤영일(Young-Il Yoon),정미연(Mi Yeon Chung),황재삼(Jae-Sam Hwang),구태원(Tae-Won Goo),안미영(Mi-Young Ahn),이영보(Young-Bo Lee),한명세(Myung-Sea Han),윤은영(Eun-Young Yun) 한국생명과학회 2014 생명과학회지 Vol.24 No.4

본 연구에서는 벼메뚜기 에탄올 추출물의 항염증 효능을 분석하기 위해 LPS로 염증 유도된 RAW 264.7 세포를 이용하였다. OCE의 항염증 효능을 확인 하기 위해서, 염증 유도된 RAW 264.7 세포에 대해 OCE 농도 의존적으로 염증성 사이토카인인 TNF-α와 IL-6의 유전자발현 및 단백질 생성을 감소시킴을 real-time PCR과 ELISA로 확인하였다. 또한, NF-κB p65의 핵으로 이동이 차단됨을 면역형광염색으로 확인하였으며, iNOS와 COX-2 단백질 발현을 감소시키는 것을 Western blot 분석으로 확인하였다. 이상의 연구결과를 통해 벼메뚜기는 염증에 의한 NF-κB p65의 활성과 TNF-α와 IL-6의 생성과 iNOS 및 COX-2의 발현을 억제하는 항염증 효능을 갖고 있는 것을 확인하였다. Although the grasshopper Oxya chinensis sinuosa has long been used as food in Korea, there is little data on its functional effects. In this study, we investigated the anti-inflammatory effect of O. c. sinuosa ethanol extract (OCE) in RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for induction of inflammation. First, we determined that there is no cytotoxicity at 2,000 μg/ml or less of OCE in RAW 264.7 cells. To evaluate the anti-inflammatory effects of OCE, we investigated expression levels of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and pro-inflammatory enzymes such as inducible nitric oxide synthase (iNOS) and cyclo-oxygenase- 2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether OCE could inhibit translocation of NF-κB p65 into the nucleus in LPS induced RAW 264.7 cells. As a result, we found that the mRNA and protein levels of TNF-α and IL-6 decreased in LPS-induced RAW 264.7 cells after treatment with OCE in a dose-dependent manner. In addition, we confirmed a 2,000 ug/ml concentration of OCE inhibited translocation of NF-κB p65 by immunnostaining and Western blot analysis, and a decrease in the protein expression levels of iNOS and COX-2. Accordingly, we suppose that OCE has an anti-inflammatory effect through down-regulation of TNF-α, IL-6, iNOS, and COX-2 related to NF-κB p65 inflammatory signaling pathways.

태반 영양모세포주의 혈관내피성장인자 분비에 미치는 Th1형 사이토카인들의 영향

정인배(In Bai Chung),최선주(Sun Ju Choi),홍민(Min Hong),최현일(Hyun Il Choi),이영심(Young Sim Lee),고춘명(Choon Myung Koh),박주영(Joo Young Park) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.5

Embryo implantation and development are critically dependent upon the regulation of angiogenesis and adequate immunologic acceptance. These local angiogenesis and vascular permeability are regulated by the interaction between fetal trophoblast, uterine decidua, and endothelial cells through the key mediator, vascular endothelial growth factor (VEGF). Problem : The mechanism through which VEGF regulation occurs at the feto-maternal interface is poorly understood. The Th1 type cytokines are known to be harmful to the successful maintenance of early pregnancy at the feto-maternal interface. Objective : To clarify whether the Th1 type cytokines could be involved in the regulation of VEGF secretion at the feto-maternal interface. Method of Study : we investigated the effects of Th1 type cytokines on VEGF secretion in human first trimester trophoblast cell-line by using reverse transcription polymerase chain reaction(RT-PCR)and enzyme-linked immunosorbent assay (ELISA). Results : The trophoblast cells expressed VEGF constitutively and the main isoforms were VEGF121 and VEGF165. When cultured in the presence of IFN-γ or IL-2, VEGF secretion was most significantly increased by IFN-γ treatment but not affected by IL-2 treatment. The level of intracellular VEGF was also increased by IFN-γ treatment. Conclusion : These results suggest that IFN-γ, despite of harmful Th1 type cytokine to the maintenance of early pregnancy, may regulate the production of VEGF in early gestational trophoblasts.

한국인 인슐린비의존형 당뇨병환자에서 엔지오텐신 1-전환효소 유전자의 다형성과 당뇨병성 만성합병증 및 관상동맥질환과의 관계

정민영,천남일,이창훈,조동혁,강호철,정동진,정명호,강정채,박종태,양승원 全南大醫大 2001 전남의대학술지 Vol.37 No.3

Study on Flow Pattern According to Rotor Design Parameter of Sewage Sludge Solid Fuel Crusher

Il‑Kab Jeong,Dong‑Myung Lee,임진택,서형윤,Chung‑Gil Kang 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.3

In coal-fred power plants, approximately 5% of sewage sludge solid fuel mixed with coal is used as power generation fuel for environmental protection and global warming prevention. The co-fring method needs a sewage sludge solid fuel crusher that can crush sewage sludge solid fuel to generate a fne powder. This study aims to determine the size of the rotor perpendicular plate hole that can minimize accumulation of the crushed fuel in the roller type sewage sludge solid fuel crusher to be used in the co-fring method. The range of the design parameters for optimization of the rotor perpendicular plate hole is selected and make an orthogonal array table according to design of experiments. The CFD is performed to analyze efect of design parameters according to an orthogonal array table. In addition, the dimension of the rotor perpendicular plate hole that can minimize accumulation of the fuel inside the crusher is drawn using the Multi-objective optimization method.

관해유도 항암화학요법에 실패한 호지킨병 환자에서 2회의 자가 말초혈액 조혈모세포이식 후 지연되어 완전 관해된 1예

정성진,김일,박환철,오호석,최정혜,이영열,김인순,최일영,안명주 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.1

관해유도 항암화학요법에 실패한 호지킨병 환자에서 2회의 연속된 자가 조혈모세포이식은 하나의 치료 대안으로서 고려될 수 있다. 저자들은 진행된 호지킨병으로 진단된 뒤 기존 항암화학요법에 저항을 보여 자가 말초혈액 조혈모세포이식을 시행하였으나 여전히 부분 관해를 보였고, 재차 자가 말초혈액 조혈모세포이식을 시행하였으나 영상학적 검사에서 부분 관해에 머물렀던 환자가 조혈모세포 이식 3년 후 추적 검사에서는 완전 관해 소견을 보인 1예를 경험하여 이를 보고하는 바이다. The double autologous stem cell transplantation after high-dose therapy has been considered as a therapeutic chance for patients with refractory or relapsing Hodgkin's disease. We report here a 29-year-old patient with Hodgkin's disease (nodular sclerosis, Stage IVB), who was partially responsive to 6 cycles of conventional chemotherapy, achieved only partial remission despite double autologous peripheral blood stem cell transplantation. However, long- term follow-up with 3 years after second stem cell transplantation, the persistently remained tumors on spleen, left ilium, and right scapular area disappeared spontaneously and now the patient has no evidence of disease. Compared with previously reported cases of long-lasting persistent evidences of unresolved disease after allogeneic bone marrow transplantation with subsequent complete response in other hematologic malignancies, an atypical pattern in this case with delayed complete response after autologous hematopoietic stem cell transplantation has not been reported.

Nonlinear Cardiac Dynamics and Morning Dip : 위험한 일중리듬 An Unsound Circadian Rhythm

염명걸,김남수,오재원,김창렬,이재웅,김순길,이철범,노정일,최정연,윤용수 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.3

韓國의 家族問題 및 家族倫理 硏究에 關한 考察

金一明,李貞德 동국대학교 대학원 1994 大學院硏究論集-東國大學校 大學院 Vol.24 No.-

This paper reviews research on family problems and family ethics in Korea. The literatures which had been published till 1994 are used to review. The review includes brief summaries of literature in research classified into three major domains : 1. A study on the traditional Korean family problems and family ethics. 2. A study on the Korean modern family problems and family ethics. 3. A study on the desirable direction of family ethics. Some of the major conclusions may be summerized as follows : 1) The ethic between man and woman, father and offspring, and the old and the young was perpendicular, authoritative and orderly, since middle of 17 Century. 2) The problems of modern society caused by industrialization. change of family ideology and religious effect. but the resarchers have focused the problems in different level. 3) The disirable direction of new family ethics established by redefinite of traditional ethics and balanced by individualism and we-feeling. It is difficult to generalize the research findings because of the short of research but it is possible somewhat to understand family problems and family ethics.

비글개에서 신원방우황청심원의 급성 및 아급성독성시험

성하정,권오경,방명주,곽형일,이진영,신대희,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of New wonbangwoohwangchungsimwon (NSCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs (3 males and 3 females) in acute experiments were administered orally with sinlge dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NSCH, 160 ㎎/㎏/day (low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NSCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NSCH in beagle dogs is considered to be safe.

구형 Helicobacter pylori의 중성구 매개 산화활성

홍원선,김혜원,이학성,명승재,정훈용,양석균,김진호,민영일,정명희 대한암예방학회 2000 Journal of cancer prevention Vol.5 No.3

精神症狀에 대한 韓國人의 態度調査

元鎬澤,洪元植,張煥日,陳聖太,金光日,李基南,嚴龍燮,金明正 大韓神經精神醫學會 1973 신경정신의학 Vol.12 No.1

Korean psychiatrists are more apt to take the abnormal drinking behavior lightly and regard the psychosomatic symptoms as more serious, comparing with American psychiatrists. On the other hand, physicians as well as adults from the population in Korea are less concerned about the neurotic and psychosomatic symptoms and alcoholic behavior as psychiatric problems. It is the authors' concern to understand such differences in opinion about abnormal behaviors from the cultural and social psychiatric view point. The authors used Dohrenwend's questionaire form of 45 mental symptoms and three additional items i. e., homosexual behavior, symptom of senile psychosis and delusion of jealousy following the authors' interests. Each item was rated by three point cale (psychiatrically very serious=2 points, somewhat serious=1, not serious at all=0) and evaluated with critical ratio. As a preliminary study, the reliability of this questionaire was confirmed by the test-retest method; Spearman's rho was 0,93. The subjects in this study consist of 50 psychiatrists, 49 physicians, 54 herb practitioners and 154 adults from the normal population(60 urban samples and 94 rural samples). The collection was carried out by individual interviews for the physicians and normal samples, by letters for the psychiatrists and the herb practitioners. And the data of American psychiatrists compared in this study were quoted from Dohrenwend's report(27). This study has two parts: one is the cross-cultural comparison between American psychiatrists and Korean ones, the other is intracultural comparison among psychiatrists, physicians, herb practitioners and normal population samples. Inspite of the general notion that Korean psychiatry has been much oriented to American psychiatry for over twenty years, there are definite differences in opinion about the severity of abnormal behaviors between American and Korean psychiatrist, especially about alcoholic behavior and psychosomatic symptoms. Such differences are possibly interpreted as arising from the cultural differences. The American psychiatrists seem to be more concerned about alcoholic behavior probably due to the strict attitude of people and/or the fact that the United States reveals the highest incidence of alcoholism throughout the world. The Korea psychiatrists, however, have much tolerable attitude toward alcoholic behavior according to the cultural tradition. Solitary drinking is hardly found in Korea; drinking in Korea is a suitable way of interpersonal contact. They drink slowly and are not so intensively dependent upon alcohol itself. Dependency need to alcohol can be diffused to the other persons. And they take alcohol with many kinds of side-dishes. These drinking manners seem to be cultural virtues for low incidence of alcoholism in Korea. Thus, Korean psychiatrists as well as adults from normal population have highly tolerable attitude toward alcoholic behavior. Even when some person reveals a destructive behavior after drinking, they grant it as a personal charm. Korea psychiatrists, therefore, are not so nervous about the alcoholic behavior. On the other hard, the psychosomatic problems seem to be serious in Korean mental patients. They are easily apt to express their emotional problems as somatic symptoms possibly due to the disease concept of oriental herb medicine that has a high tendency of projection of emotional conflicts unto the soma. So most of Korean people are not aware that psychosomatic symptoms are originated from the emotional problems, and quite contrary to it, the psychiatrists may be highly conscious of psychosomatic symptoms. Another significant difference is seen in suicidal idea and suspiciousness: American psychiatrists regard them as more serious, and reversely Korean psychiatrists regard them as less serious. Such a pattern may also be derived from the socio-cultural differences. When Koreans are either dissapointed or delighted, they say frequently "I want to die" or "It's funny to death" and so on. This idiomatic expression is far from the real death wish but rather an expression of magnification. And the suicidal idea itself is frequently seen in normal population even suicidal attempt is evaluated as abnormal in Korea. With such reasons, Korean psychiatrists may notice suicidal idea as less serious than American psychiatrists. And the suspiciousness may be a way of living for the people under the historical turmoil and vigorous aculturation, that it is perceived as not so abnormal. The next problem is the marked discrepancy of opinion about abnormal behaviors among psychiatrists, physicians, herb practitioners and normal population samples. The agreement of physicians, herb practitioners, urban samples and rural samples with the psychiatrists on the relative severity of symptoms revealed as Spearman's rho of 0.79, 0.59, 0.58 and 0.44 respectively. It is the main point of problem that physicians are less aware of neurotic symptoms which can appear in the somatic illness, and of psychosomatic symptoms as psychiatric problem. Possible reason is that Korean physician have been oriented to the somatic medicine without proper psychosomatic orientation. On the other hand, the herb practitioners are apt to ignore the severity of psychotic symptoms and on the other, regard the neurotic and psychosomatic symptoms as much as psychiatrists' opinion. And the samples of normal population regard the neurotic symptoms as more serious than the psychotic symptoms, and are not aware of the psychosomatic symptoms as psychiatric problems. Physicians, herb practitioners and samples from the normal population underestimate the alcoholic behavior more than psychiatrists do. The alcoholism according to physicians' opinion is rather somatic problem than psychological one. The herb practitioners and adults from normal population manifest the prominently tolerable attitude toward alcoholic behavior. In conclusion, two kinds of problems can be suggested. One is that the difference between Korean and American psychiatrists in the evaluation on the severity of abnormal behaviors, is possibly due to sociocultural differences. The other is the intracultural difference among psychiatrists, physicians, herb practitioners and adults from normal population. Such a difference suggests that there is a considerable cultural lag in the disease concept and attitude between the modern psychiatry and the cultural tradition. This discrepancy can result in the undesirable wandering of patients for seeking the treatment and some hardship in modern psychiatric practitioning in Korea.