간 세포암에서 VEGF, TGF-β1, b-FGF 발현의 의의

김성용,남충현,주종우,채만규,백무준,이문수,김형철,안현철,김홍수,김창진,김창호 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: Angiogenesis is important for the proliferation and the metastasis of solid tumors. The growth of a solid tumor is widely recognized to depend on the process of neovascularrozation. Without angiogenesis, tumors cease to grow beyond even a few milimeters in diameter. It has been shown that tumor vascular density is an independent prognostic marker in several types of human tumors and is known to correlate with poor prognosis. To date, many angiogenic factors have been identified, such as transforming growth factor-α(TGF-α), transforming growth factor-β(TGF-β), fibroblast growth factor family(FGF), vascular endothelial growth factor(VEGF), platelet derived endothelial cell growth factor(PD-ECGF), tumor necrosis factor-α(TNF-α), and angiogenin. Hepatocellular carcinoma(HCC) is the second most common tumor in Korean males and is known as a typical hypervascular tumor with frequent portal vein invastion. The authors identified the expreesion of VEGF, TGF-β1, and b-FGF in HCC specimens and evaluated the relationship between these growth factors and the clinicopathologic characteristics of HCC. Method: We reviewed the medical records of 30 patients who were diagnosed as hepatocellular carinoma treated with hepatic resection between January 1994 and December 1998 in Soonchunhyang University Chunan Hospital. The selection of the cases was decided according to the condition of paraffin block fixation. The prognostic factors such as age, sex, tumor size, concentration of serum α-fetoprotein, presence of liver cirrhosis, presence of tumor emboli in portal vein, TMN stage, amount of transfusion during the operation, hepatitis B virus(HBV) infection, and Edmonson-Steiner(E-S) grade were investigated. Relationship between the prognostic factors and the immunopathologic expression of the TGF-β1, b-FGF, and VEGF was examined. Result: Thirty patients (24 males, 6 females) were included in the current study. The patient's mean age was 50.6 years and the age ranged from 36 to 65 years. The mean size of the tumor was found to be 5.2cm. All the patients were follewed up for 7 to 63 months. Child's classification A patients were 23(76.7%)cases, B patients were 7(23.3%)cases, and C was none. Immunohistochemical staining of HCC tumor mass in VEGF expression patients were 17(56.7%), b-FGF expression patients were 10(33.3%), and TGF-β1 expression patients were 10(33.3%). VEGF expression or more than one positive expression among the three factors correlated with tumor size and the stage of HCC but did not correlated with other clinicopathological characteristics. TGF-β1 and b-FGF did not correlate with any clinicopathological characteristics. Conclusion: The results suggest that the expression of VEGF or more than one positive expression among the three factors in HCC cells may be a significant prognostic factor of HCC.

급성 임파성 백혈병의 말초혈액 검사소견

김성집,안경남,조무자,김춘식,심상인,김선무 대한임상병리사협회 2001 대한임상병리사회지 Vol.21 No.1

대학생의 노인복지에 대한 인식도

차병준,김무룡,김기열,차경미,김귀희,김현실,서인선,임상규,이순자,위광복,남철현 慶山大學校 保健福祉硏究所 1999 保健福祉硏究 Vol.2 No.-

The purpose of this study is to grasp the change of consciousness of young people in order to seek new approach of policy for aged era. 1,200 students attending collages and universities in three small or medium sized cities were inquired for 2 months, from October 1 to November 30, 1996. The result is as follows. 1. The characteristic of the respondents of this survey is that they include 72.1% of women, 40.4% of people aged between 20 to 21, and 49.1% of people who do not have any religion. Those who are from big cities and those who are form farming villages or fishing village occupy equally 40.2%, those who reside in that cities for a long period 49.0%, and those who live with their family 60.9%. 2. The family feature of the subject is that 31.5% of them are those whose father is the age of 55 and more than 55, 10% of them are those whose mothers age is so, 33.1% of them have 5 family members, 29.5% of them have the family income of 1,000,000 to 1,490,000 won, 83.9% of them are from middle class, 47.7% of them are the people whose grand parents have passed away, 70.4% of them live apart from their grand parents, and 60.9% of them are second child. 3. 64.0% of males, 70% of respondents who are aged over 24, 58.1% of respondents who are from rural communities, 62.4% of respondents who live in rural area, and 58.3% of respondents who live alone, prefer to live with grand parents, marked higher rate than other groups. The groups whose father and mother are the age of more than 55 marked higher rate of 55.9% and 58% each than any other groups(p<0.05). The groups whose family member is less than three, whose grand parents both have passed away in middle class, who live with their grand parents, and who are second children, give more positive answers than any other groups. 4. Those who are older, who are buddhists, who are from rural area or live in rural area, whose family is small, whose family income is small, who are from lower class and whose grand parents are still alive, shows preference to support their parents after marriage. 5. Females(89.4%), those who are from small or medium sized cities(89%), who live with their family(85.6%), whose father is the age of 55 and more than 55, whose mother is the age of 54 and less than 54, whose family member is 6, who are from middle class, whose grandparents are still alive and who are the eldest child in their family, give more response that they prefer to live apart from their children in their old age than any other groups. 6. What elderly people need most right now is past time(42.3%), and the respondents whose age is 20 to 21, who are buddhists, who are from middle sized cities and live alone, whose parents are the age of more than 55, who have small family income, who live with their grand parents and who are second children show higher level than any other groups. 7. 76.8% of respondents answer that they do not need their parent's fortune, marked still higher rate than the opposite answer. Those who are older, who are buddhists, who are from big cities, who reside in rural area, who live alone and have smaller family, who are in bad family economic conditions and who live with grand parents present higher rate of positive response. 8. 59.3% of respondents reply that they think about death from time to time, and those who are female, who are in the age of 20 to 21, who are christian, who live with their family, whose siblings or other family members offer their education expenses show higher rate than any other groups. 9. 92.3% of respondents answer that the budget for welfare facility for elderly people is very small. The younger the respondents are and the worse economic condition they have among those who live in big cities, the more answered that. 10. 50% of respondents think social security should provide for their old age, and 42.8% of them believe they themselves provide against it. The respondence that social security ought provide for it appeared more among those whose age are over 24, who are female, who reside in rural area, who are christian, who are in bad economic condition, and who ever used medical institutions in recent one month, marked higher level than other groups. 11. The adequate time of retirement is 59.97±5.60 for public officers, the highest and 59.28±6.35 for teachers, the next. 12. 35.2% of respondents reply that the adequate budget for the old age after their retirement at the age of 60 is 220 million won, showed the highest level of respondence. 13. The factors which affect on the old people in the charged welfare facilities are sex(p<0.01), the type of housing(p<0.05) and whole family income(p<0.05). 14. The factors which affect on remarriage are sex(p<0.05) and religion(p<0.05). For varying old population and expanded welfare service for them, government and relevant authority should give more attention to secure the budget, establish effective plans to expand various program and the facilities for elderly people in order that elderly people spend the rest of life time more happily and more fruitfully. To do that, each college and university should open Health courses, and educate and inform about health and welfare.

산업근로자의 식생활 의식행태 조사

김무룡,황연자,차경미,이순자,위광복,남철현,김기열,김홍길 慶山大學校 保健福祉硏究所 1997 保健福祉硏究 Vol.1 No.-

This study was carried out to grasp the awareness and habits of food intake and use the information for health education program in order to help healthy dietary life. The survey was done in cooperation with health manager in industries on 1,200 workers who was selected randomly from 5 types of industries from Sep. 2 to Oct. 31, 1995. The results are summarized as follows: 1) The percentage of subjects who assess themselves "Healthy" was more higher in female, in 20∼29 age group, in single, in daytime workers, in college graduate than other groups respectively. 2) As for the degree of health education satisfaction, 35.5% of the subjects answered "dissatisfaction" 3) As for the exercise, 52.7% exercised for their health and the rate of exercise was more higher in male, in 30 years age group, in single than other groups respectively. 4) The rate of smoking was 57.6% and the rate of drinking alcohol was 47.3%. 5) 92.8% of the subjects got stress everyday and the rate is more higher in female, in less than 19 years old, in single, in longer working duration, in lower income, in high school graduates, in workers of product field line than other groups respectively. 6) As for drinking times of coffee or soft drink, two times per day was 29.5%, more three times per day was 28.0%. As for the eating speed of meal, 46.0% of the subjects answered "fast" and the rate was more higher in male, in 30 years age group, in married group, than other groups respectively. 7) With regard to the level of preference to instant food, 23.1% preferred to take instant food. As for the level of preference to hot and salty food, 28.1% preferred to have hot and salty food. 8) The score of eating behavior was 3.38±32 point to 6 point full marks. 9) With regard to the correlation among related variables, the variables of gender, marital status and working duration had positive relation with the variables of regular meal intake, the preference to food intake between regular meals.

당귀의 추대에 관한 연구

김무남 관동대학교 1972 關大論文集 Vol.1 No.1

1) This stud has done to find the influence on the yield and bolting of medicinal Dangui. We classified the seeding time into four periods; 15th March, 4th April, 24th April and 14th May. We also classified the transplanting time into four periods; 5th October, 5th November, 15th March and 15th April of the next year. 2) The seeding time showed little influence on the yield and bolting. However, in the transplanting time, the plot of 5th October, being small bolting and increasing yields, is the best and the plot of 15th March is the next. 3) There was a significant correlation between the size of transplanting seedling and rate of bolting. Thus, we found that the rate of bolting becomes small in proportion to the size of seedling. 4) In cultivation of Dargui, we had better plant in April or May and transplant in March of the next year. Relatively small ones and the same size of seedling are recommended.

초기계태에서 valproic acid에 의한 신경관 기형 : 입체현미경적 관찰

김동호,조무연,정유남,최영주,이종선,민경수,이무섭 충북대학교 의학연구소 2000 忠北醫大學術誌 Vol.10 No.1

연구목적 ' 항경련제인 valproic acid가 초기계태의 신경관 형성에 영향을 주는 기형유발에 대해 입체현미경하에서 관찰하였다 연구재 료 및 방법 : 백색의 Leghorn의 신선한 수정란을 30-35시간 동안 배양하여 Hamburger & Hamilton 기 5-10기의 초기 계태를 천공 필터를 이용하여 분리하여 6-30시간 동안 CO2 세포 배양기에서 배양하였고 무작위로 대조군과 실험군을 분류하였다. 실험군은 valproic acid 의 RPMI culture media 의 농도에 따라 100 ㎍/㎖, 200 ㎍/㎖, 500 ㎍/㎖, 1000 ㎍/㎖ 네가지 군으로 분리하였다. 각각의 대조군과 valproic acid를 처치한 실험군에서 입체 현미경하에서 초기계태의 신경관 형성의 형태학적인 특징과 기형형성의 빈도를 비교하였다. 결과: valproic acid가 없는 RPMI media에서 배양된 대조군은 24개의 계태는 22(91.7%)개는 정상으로 자랐으며 2(8.3%)개는 비정상으로 자랐다 이에 반하여 valproic acid가 처치된 RPMI media에서 배양된 72개의 계태는 42(58.3%)개가 비정상으로 자랐다. 주로 확인된 이상 형태는 신경주름의 변형. 신경관 폐쇄부전, 체절의 장애 및 발육 정지 등 이었다. 계태의 기형 형성은 valproic acid의 농도에 비례하여 증가하였다. valproic acrid 의 농도가 100 ㎍/㎖인 경우 18개 계태 중 2(11.1%)개에서 경미한 신경관 기형이 나타났으며 200 ㎍/㎖인 경우 18개 계태 중 11(61,1%)개는 정상, 7(38.9%)개는 명백한 신경관 기형이 나타났으며 500 ㎍/㎖인 경우 18개의 계태 중 5(27.8%)개는 정상. 13(72.2%)개는 명백한 신경관 기형을 나타냈다. 1000 ㎍/㎖인 경우 18(100%)개 모두 심한 신경관 기형이 나타났다. 결론: valproic acid는 초기계태에서 신경관 기형을 유발 했는데 저농도에서는 일부 경미한 신경관 이상을 보였고 고농도에서는 대부분 심한 신경관 손상을 동반하고 성장 속도가 둔화되어 약 용량의 증가에 따라 기형이 증가되는 dose-dependent manner 로 그 영향이 나타났다. Purpose : The teratogeruc effects of valproic acid widely used as anticonvulsant on the neurulation of the explanted eraly chick embryos were observed by the stereoscope. Materials and Methods: Fresh fertilized white leghorn hen eggs were incubated for 30-35 hours in an e99 incubator. The Hamburger and Hamilton stage 5-10 chick embryos were explanted using the punched-out filter paper explantation technique and cultured in the CO2 cell culture incubator for 6-30 hours. They were randomly divided into a control group and an experimental group. The experimental group was divided into four subgroups according to the valproic acid concentrations of 100 ㎍/㎖, 200 ㎍/㎖, 500 ㎍/㎖, 1000 ㎍/㎖ with which the RPMI culture media were treated. The morphological characteristics and the incidences of teratogenic effects on the neurulation of early chick embryos in the control and experimental groups were compared with each other using the stereomicroscope. Results : Of the 24 chick embryos cultured in the RPMI media without valproic acrid, 22 embryos(91.7%) developed normally, and 2 embryos (8.3%) developed abnormally, in contrast, among 72 embryos cultured in the valproic acrid-treated media, 42 embryos(58.3%) developed abnormally. The frequent anomalous features were deformities of the neural folds, failure of neural tube closure, derangement of somites, and developmental arrest. Of 18 embryos cultured in the media treated each with 100㎍/㎖ of valproic acid, 2 embryos(11.1%) showed mild abnormality of neural tube defect on stereoscopic examination. Of 18 embryos cultured 7l the media treated each with 200㎍/㎖ of valproic acid, 11 embryos(61.1%) developed normally for 20 hours and 7 embryos (38.9%) showed definite neural tube defect. Of 18 embryos cultured in the media 7reated each with 500㎍/㎖ of vaIprolc acid, 5 embryos(27.8%) developed normally (or 20 hours and 13 embryos (72.2% ) showed definite neural tube defect. Of 18 embryos cultured in the media treated each with 1000㎍/㎖ of valproic acid, 1 embryo developed normally for 4 hours but all embryos (100%) showed severe neural tube defect on whole brain after 8 hours of culture. Conclusion : Valproic acid induced mild neural tube defects in low concentration and in high concentration of valproic acid, most chick embryos developed slowly and showed severe neral tube defects. The frequency and severity of abnormal embryos ulcreased in dose-dependent manner.

조립 Filter내에서 세상의 이동현상에 대한 비교분석

金顯起,權武男 慶北大學校農業科學技術硏究所 1998 慶北大農學誌 Vol.16 No.-

본 실험에서는 수리구조물의 보호되는 토립자의 필터내 이동과 이로인한 필터의 기능 저하를 실험을 통하여 비교분석한 결과 다음과 같은 결론을 얻었다. 1. 수두가 높고 공극이 클수록 필터내 세사의 이동은 심했으며 조기에 흐름의 안정화에 도달하게 되었지만 필터외부로의 입자유출도 많았으며, 공극이 작고 유속이 빠르면 필터와 모래의 접촉면에 인접한 곳에서 Blocking현상이 크게 일어남을 알 수 있었다. 2. 필터기능에 영향을 주는 세사의 이동은 공극의 크기와 수두에 큰 영향을 받음을 알 수 있었고, USCE 필터와 USSPL 필터는 공극이 크기 때문에 같은 조건하에서 흐름의 안정이 다른 필터들 보다 조기에 이루어 졌다. 3. 세사의 이동잔류량은 USSPL, USCE, USBR, Newton & Hurley, Bertram 필터순으로 많이 나타났다. 4. 흐름의 안전에 소요된 시간은 Bertram, Newton & Hurley, USBR, USSPL, USCE 필터 순으로 적었고, 투수계수는 USBR, Bertram, Newton & Hurley, USSPL, USCE 필터순으로 크게 나타났다. 5. 여러 가지 종류의 필터기준중 USCE와 USSPL기준의 필터가 투수계수의 안정에 소요된 시간이 가장 짧고, 세사의 이동량이 적으므로 가장 적합한 것으로 인정된다. This experiment did comparison and analysis that protected soil particle migration have affect on function of the filter and therefore fall function of the filter. Results obtained are as follows: 1.High water head makes to be much movement of fine sand and out flow of particle to the outside. The filter have large opening size that reached stability an early stage, but much fine sand is washed away. If the velocity turns fast and becomes small, blocking phenomenon is remarkable nearby the filter-sand interface. 2. The movement of fine sand that effect on function of filter depend on opening size and change of water head. Under the same condition, USCE filter and USSPL filter is reached earlier than other filter that is stability of stage, because it's opening size is large. 3. Residual quantity of fine sand migration was largly come out in order of USSPL, USCE, USBR, Newton & Hurley, Bertram filter. 4. The time required to stability of flow was taken less in order of Bertram, Newton & Hurley, USBR, USSPL, USCE filter and coefficient of permeability was highly come out in order of USBR, Bertram, Newton & Hurley, USSPL, USCE filter. 5. It proved that USCE and USSPL is suitable for the filter criteria.

소 卵胞의 體外培養에 의한 卵子의 體外成熟과 體外受精에 관한 硏究

金相根,南潤伊,山根倫子,金武剛 충남대학교 수의과대학 동물의과학연구소 1997 動物醫科學硏究誌 Vol.5 No.-

This study was to investigate the effects of the follicular culture from which the oocytes origenate on their subsequent in vitro maturation. The follicular oocytes were cultured in TCM-199 medium containing FCS, hormones, BFF for 24-30 hrs in CO^2 incubator with 5% CO^2 in air at 38.5℃ and then matured oocytes were again 18-20 hrs with motile capacitated spermatozoa in the BO solution containing 100 ㎍/㎖ of heparin. The results obtained in these experiments were summarized as follows : 1.Ovarian follicles were isolated and cultured according to size 1-2 ㎜, 2-4 ㎜, 4-6 ㎜ and 6-8 ㎜ for 24-30 hrs. The rates of GVBD in each group were 80.0%(40/50), 84.4%(38/45), 77.8%(35/45) and 84.0%(42/50), but oocytes maturation were supressed at anaphaes-Ⅰ or telephase-Ⅰ stage. 2. When oocytes cultured for 4 hrs at 35℃ in medium with BFF and hormones after follicular culture for 24-30 hrs, the maturation completed to metphase-Ⅱ stage. However, rates of GVBD in oocytes from follicular culture were higher than oocytes cultured in medium. 3. When oocytes from follicle cultured for 4 hrs at various temperature and culture time were cultured for 24-30hrs the rates of oocytes maturation from follicle cultured at 20℃(43.6%, 24/55), 30℃(47.3%, 26/55) and 35℃(52.7%, 29/55) were significant higher than group cultured at 4℃(24.4%, 11/45). 4. When oocytes from follicles cultured for hrs at 35℃, the rates of oocytes maturation and fertilization rates were 67.3%(101/150), 29.3%(44/150) and oocytes cultured for 24-30hrs at 35℃, thr rates of oocytes maturation and in vitro fertilization were 86.5%(122/141), 53.9%(76/141), respectively.

飼料作物 Alfalfa(Medicago sativa L.)品種別 生育狀에 대하여

김무성,노영덕,김세영,백남인,정우진 경희대학교 부설 식량자원개발연구소 1996 硏究論文集 Vol.17 No.-

서로 특성이 다양한 32개의 도입한 Alfalfa품종을 우리나라 중부지역인 충청남도에 재배하여 초장과 건물중 등의 생육상태를 비교, 검토한 결과는 다음과 같다. 1. 평균초장이 큰 상위 10개 품종들은 Dekalb brand 135 > Diamond > Pioneer brand 581 > Raidor > Pioneer brand532 > Pierce> Nitro > Sparta ) Florida 77 > Sapphire의 품종순 이 며, 초장이 작은 10개 품종을 최하위 순으로 열거하여 보면 Salute < Spredor 2 < LS-1920 < Arrow < Challenger < Milkmaker < Armor < Pioneer brand 545 < Pioneer brand 5262 < Saranac의 품종 순이였다. 2. 총건물 수량이 많은 상위 10개 품종들은 Diamond > Nitro > Dekalb brand 120 > Dekalb brand 135 > Drummer > Saranac > Sapphire > Raider > Magnum > Dekalb brand 167의 품종 순이며, 건물수량이 적은 최하위 순으로 10개 품종은 Milkmaker < Pioneer brand 526 < Pioneer brand 5262 < Salute < Pioneer brand 555 < Decathlon < Pioneer brand 5929 < LS-1920 < Pioneer brand 581 < Husky의 품종들이였다. 3 평균초장과 총건물 수량의 결과를 비교하여 보면 대체로 초장이 우세하였거나, 열세하였던 품 종들이 다소간 건물수량이 우세하거나 열세하였으며, 정비례한 결과는 보이지 아니하였다. 4. 생육상태 및 건물수량이 모두 좋은 품종들은 Diamond, Nitro, Dekalb brand 135, Sapphire, Raidor품종들이며, 우리나라 중서부지역의 재배환경에 적합한 품종으로 사료된다 This study was conducted to select the best adapted alfalfa cultivars among the 32 introduced alfalfa cultivars with different characteristics in Chung-Chong Nam Do, Korea, environmental condition. Mean of plant height and total dry matter yields per cultivar from May to August in 1996 were determined. On the point of mean of plant height, ten high ranked cultivars among the 32 alfalfa cultivars in high order were Dekalb brand 135>Diamond>Pioneer brand 581>Raidor>Pioneer brand 532>Pierce>Nitro>Sparta>Florida 77>Sapphire and ten low ranked cultivars in low order were Salute<Spredor 2<LS-1920<Arrow<Challenger<Milkmaker<Armor<Pioneer brand 545<Pioneer brand 5262<Saranac. Ten high ranked cultivars from 32 alfalfa cultivars on the total dry matter yields in high order were Diamond>Nitro>Dekalb brand 120>Dekalb brand 135>Drummor>Saranac>Sapphire>Raidor>Magnum>Dekalb brand 167 and ten low ranked cultivars in low order were Milkmaker<Pioneer brand 526<Pioneer brand 5262<Salute<Pioneer brand 555<Decathlon<Pioneer brand 5929<LS-1920<Pioneer brand 581<Husky. Total dry matter yields of per cultivar was also slightly greater but It was not appeared direct proportion when alfalfa cultivar was appeared higher mean of plant height compared with those which were lower mean of plant height. Leading alfalfa cultivars for plant growth and dry matter yields were Diamond, Nitro, Dekalb brand 135, Sapphire, Raidor cultivar which can be well grown under moderate temperatures zone of midwest in Korea.

포도 접목에 영향을 미치는 몇가지 생장조절제의 효과에 관한 연구

김무남 관동대학교 1975 關大論文集 Vol.3 No.1

1) I treated many kinds of plant growth regulator on the grape grafting order in to raise the rate of graft union survivals. Among many kinds of plant growth regulator there are IAA solution block, NAA solution block, IAA lanolin block and lanolin ointment block, etc. Making an experiment with IAA solution block and NAA solution block, the scion which is soaked in IAA solution block and NAA solution block is grafted on the stock. And I applied IAA solution block and lanolin block to the point of grafting. 2) In the formation of callus and the rate of sprouting, IAA solution block and NAA solution block are more effective than control, but IAA solution block and lanolin ointment block are little effective. 3) The rate of rooting is little difference among the treatment block, so it is imagined the scion which is treated by growth regulator don`t have an effect upon the root from coming out stock. 4) In the rate of graft union survivals, non-treatment block is 41% on the other hand IAA solution block is 67%, NAA solution block is 64%, which is imagined to be a very effective method. But two blocks used by industrival lanolin have so bad effective that it seems to be any kinds of harmful things in lanolin.