Radicicol Inhibits iNOS Expression in Cytokine-Stimulated Pancreatic Beta Cells

Cha Kyung Youn,Seon Joo Park,Mei Hong Li,Min Young Lee,Kun Yeong Lee,Man Jin Cha,Ok Hyeun Kim,Ho Jin You,In Youp Chang,Sang Pil Yoon,Young Jin Jeon 대한생리학회-대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Here, we show that radicicol, a fungal antibiotic, resulted in marked inhibition of inducible nitric oxide synthase (iNOS) transcription by the pancreatic beta cell line MIN6N8a in response to cytokine mixture (CM: TNF-Ձ, IFN-Ճ, and IL-1Ղ). Treatment of MIN6N8a cells with radicicol inhibited CM-stimulated activation of NF-ՊB/Rel, which plays a critical role in iNOS transcription, in a dose-related manner. Nitrite production in the presence of PD98059, a specific inhibitor of the extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) pathway, was dramatically diminished, suggesting that the ERK1/2 pathway is involved in CM-induced iNOS expression. In contrast, SB203580, a specific inhibitor of p38, had no effect on nitrite generation. Collectively, this series of experiments indicates that radicicol inhibits iNOS gene expression by blocking ERK1/2 signaling. Due to the critical role that NO release plays in mediating destruction of pancreatic beta cells, the inhibitory effects of radicicol on iNOS expression suggest that radicicol may represent a useful anti-diabetic activity.

Optimization of Electric Field-assisted Ultrasonication for Disintegration of Waste Activated Sludge

( Kyung-won Jung ),( Tae-un Jeong ),( Min-jung Cha ),( Kyu-hong Ahn ) 한국폐기물자원순환학회(구 한국폐기물학회) 2014 한국폐기물자원순환학회 심포지움 Vol.2014 No.1

In the present study, an electric field is applied in order to disintegrate waste activated sludge (WAS). As a preliminary step, feasibility tests are investigated using different applied voltages of 10 to 100 V for 60 min. As the applied voltage increases, the disintegration degrees (DD) are gradually enhanced, thereby the soluble N, P, and carbohydrate concentrations increase simultaneously due to the protein decomposition. Subsequently, an optimization process is conducted using response surface methodology with a Box-Behnken design (BBD). The total solid concentration, applied voltage, and reaction time are selected as independent variables, while the DD is selected as the response variable. The overall results demonstrate that the BBD with an experimental design can be useed effectively in the optimization of the electric field treatment of WAS. In the confirmation test, a DD of 10.26±0.14% is recorded, which corresponds to 99.1% of the predicted response value under the statistically optimized conditions. Finally, the statistic optimization of the combined treatment (electric field + ultrasonication) showed that even though this is limited to highly disintegrated WAS when being applied individually, a high DD of 47.28±0.20% was recorded where the TS concentration was 6,780 mg/l, the strength of ultrasonication was 8.0 W, the applied voltage was 68.4 V, and the reaction time was 44 min. E-SEM images clearly revealed that the application of the electric field is a significant alternative method for the combined treatment of WAS, and this is first attempt to increase disintegration using electric field for combined treatment with ultrasonication.

Radicicol Inhibits iNOS Expression in Cytokine-Stimulated Pancreatic Beta Cells

Youn, Cha Kyung,Park, Seon Joo,Li, Mei Hong,Lee, Min Young,Lee, Kun Yeong,Cha, Man Jin,Kim, Ok Hyeun,You, Ho Jin,Chang, In Youp,Yoon, Sang Pil,Jeon, Young Jin The Korean Society of Pharmacology 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Here, we show that radicicol, a fungal antibiotic, resulted in marked inhibition of inducible nitric oxide synthase (iNOS) transcription by the pancreatic beta cell line MIN6N8a in response to cytokine mixture (CM: TNF-${\alpha}$, IFN-${\gamma}$, and IL-$1{\beta}$). Treatment of MIN6N8a cells with radicicol inhibited CM-stimulated activation of NF-${\kappa}B$/Rel, which plays a critical role in iNOS transcription, in a dose-related manner. Nitrite production in the presence of PD98059, a specific inhibitor of the extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) pathway, was dramatically diminished, suggesting that the ERK1/2 pathway is involved in CM-induced iNOS expression. In contrast, SB203580, a specific inhibitor of p38, had no effect on nitrite generation. Collectively, this series of experiments indicates that radicicol inhibits iNOS gene expression by blocking ERK1/2 signaling. Due to the critical role that NO release plays in mediating destruction of pancreatic beta cells, the inhibitory effects of radicicol on iNOS expression suggest that radicicol may represent a useful anti-diabetic activity.

한국판 대칭 및 정리정돈 성향 척도(K-SOAQ)의 표준화 연구

차경렬,임준석,박준영,이수영,김광욱,노대영,김세주,구민성,김찬형 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.3

Objectives : The Symmetry, Ordering and Arranging Questionnaire (SOAQ) has been reported as a valid tool for the assessment of symmetry, ordering and arranging compulsive behaviors of patients with obsessive compulsive disorder. It was introduced by Radomsky and Rachman in 2004. The aim of this study was to assess the reliability, validity and psychometric properties of the Korean-version of the SOAQ (K-SOAQ). Methods : Two hundreds twenty eight under-graduate college students were enrolled in this study. They were assessed with K-SOAQ, Obsessive-Compulsive Inventory-Revised (OCI-R), Beck Depression Inventory (BDI) and Beck Anxiety Inventory (BAI). Statistical analysis was done using calculation of Cronbach’s alpha, Spearman Correlation Coefficient and Principal Components Analysis. Results : There were no significant differences in sex, education, and total scores of BDI and BAI. The Cronbach’s alpha coefficient of K-SOAQ was high, Cronbach’s α=0.96. There was strong evidence for a one-factor solution, accounting for 60.3% of the variance. Convergent validity was examined through correlations between the SOAQ and the OCI-R (r=0.61). Divergent validity was examined through correlations between the SOAQ and two self report measures; BDI (r=0.12) and BAI (r=0.19). Conclusion : The K-SOAQ showed good reliability and validity for the assessment of severity of compulsive characteristics regarding symmetry, ordering and arranging. The K-SOAQ is a useful instrument for assessing compulsive symptoms related with symmetry, ordering and arranging in Korea.

Haemophilus aphrophilus 심내막염 1예

차충환,신희봉,장성수,김민경,김양수,송재관,김미나 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

영어 청취력 단계별 문항연구

차경환,이경민 中央大學校 韓國敎育問題硏究所 1994 韓國敎育問題硏究所論文集 Vol.- No.9

The purpose of this study was to investigate items of English listening materials and to analyze them according to the learners' levels of proficiency and the kinds of listening processes. Learners were divided into three levels (beginning, intermediate, and advanced level), and the listening processes were also categorized into three kinds (top-down, bottom-up, and interactive process). Consequently, the listening items were subdivided into nine classes. Nineteen listening textbooks were used for the analysis. Each listening item was given explanations of the aim, task, characteristic, type of listening process and some advice applicable to study. The use of listening items appropriate for the learners' level and target listening process can find the maximum efficiency in studying listening. The results presented here can be widely used in designing listening materials and listening test items for the Korean Scholastic Aptitude Test for college entrance.

일부지역 노인들의 주관적 건강수준에 영향을 미치는 요인에 관한 연구

민경진,김근조,차춘근 韓國保健敎育.健康增進學會 2001 보건교육건강증진학회지 Vol.18 No.2

This research is aimed to define how the depression, performing ability of IADL and muscular-skeletal pain of the Aged, according to their residential circumstance, sex and age, can affect the subjective health index and how all these are related and associated with. For the period of June 1 to July 31,2000, in order to study and define how the depression, performing ability of IADL and muscular-skeletal pain are related to the subjective health index of the Aged, we have conducted an enquete through a direct interview with 693 persons over age sixty-five (65) in Daejon and in other adjacent areas, divided into three different residential types "The Aged living at home", "The Aged living at welfare facilities" and "The Aged living alone". We have studied all the data and information obtained through this enquete and have analyzed χ2_test, t-test, ANOVA, analysis of simple correlation, analysis of factor. by SPSS10.0/PC+. The results were as follows : Generally, the four (4) factors depression, IADL performing ability, muscular-skeletal pain and the subjective health index of the Aged, are a lot influenced by and related to their residential circumstance, their sex and their age. With regard to the depression of the Aged by their sex, it was analyzed that, on an average, the female-Aged gains 48.3 point which reflects comparatively the higher points and the female-Aged is more depressed. In analyzing depression of the Aged by their age, it appears that the Aged between eighty (80) to eight-four (84) years of age, gains 49.2 point which is the highest points and simultaneously we could realize that depression follows age, - the more the age gets, the more the depression is increased. In analyzing depression of the Aged by their residential type, it shows that "the Aged living alone" gains 50.9 point and is most depressed. With regard to the IADL performance of the Aged by their sex, it was analyzed that, on an average, the female-Aged gains 23.8 point, which shows the performance of the female-Aged is less independent. In addition, it was also found that the IADL performing ability is becoming less and less independent following their age increasing. In analyzing IADL performance by their residential type, it appears that "the Aged living at welfare facilities" gains the lowest 21.5 point and is least independent. We conclude that some assistances from others are required for the Aged living at welfare facilities in their performing IADL. With regard to the muscular-skeletal pain of the Aged by their sex, it was analyzed that, on an average, the female-Aged gains 3.0 point and the female-Aged surfers from this pain more severely. In analyzing this pain by their residential type, it was found that, on an average, the 3.0 point goes for "the Aged living alone", which explains the Aged living alone is having the most serious pain. With regard to the subjective heath index of the all Aged participated in this research, the analysis indicates 8.8 point and this is considered as a general standard (7-10 point). In analyzing this index by their sex, the female-Aged gains 8.6 point only and it explains a lot of female-Aged consider they are not really healthy. In analyzing this index by their residential type, "the Aged living at welfare facilities" and "the Aged living alone" gain the comparatively lower point, - respectively 8.4 point for the Aged living at welfare facilities and 8.8 point for the Aged living alone. The Aged of these two residential types express they are obviously in a bad condition of health, which makes us think a lot. With regard to the factors affecting the subjective health index of the Aged, it was analyzed that this index can significantly be influenced by their depression, their pain, their age and by how much they are satisfied with their current living conditions, and also analyzed that the correlative relation certainly exists between the depression and pain, and the subjective health index, - that is, the more the depression and pain are serious, the lower the subjective health index indicates. As for the IADL, it appears that the IADL's relation with this index is not that significant and even not important.

GHP용 엔진의 개발 연구 (Ⅰ) : 저속·고출력 천연가스 엔진의 개발 The Development of Low speed·High Power Natural Gas Engine

이민호,차경옥 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

GHP, operated by gas instead of electrical motor, has there is better energy saving 20% comparing with EHP(electrical motor-driven heat pump) by using the exhaust heat from the engine effectively. This means that GHP has an advantage in the reduction of CO_2 which causes main greenhouse effects. Natural gas is one of the most promising alternative fuels for automotive vehicles, because it has lower exhaust emissions and better fuel economy characteristics than gasoline, diesel, LPG and can be used in conventional engine with a little modifications. Natural gas engine for GHP should be mostly based on 4 stroke water-cooled diesel engine which has high power in low speed, but sometimes could be based on automotive LPG engines because of easy modification. In this paper, a conventional LPG engine was modified to a NG dedicated engine, which can be operated with NG only, and a engine bench test was performed at various experimental condition. As a result, it was revealed that the prototype NG engine can be operated with lower exhaust emissions, better fuel economy and better thermal efficiency, but with a slightly reduced brake horse power, compared to the conventional LPG engine.

애엽, 지각, 아교주 및 목향의 투여가 임신중 생쥐의 생식능력에 미치는 효과

정형민,차수경,임정묵,신태은,박찬,장준복,이경섭,송병기 경희대학교 2000 慶熙醫學 Vol.16 No.2

Preangulated TMA T-loop spring의 적용 위치 변화에 따른 견치의 초기 응력 분포에 대한 유한 요소법적 연구

김정민,차경석,이진우 대한치과교정학회 1999 대한치과교정학회지 Vol.29 No.5

본 연구는 상악 견치, 상악 제 2 소구치, 상악 제 1 대구치의 유한요소 모델을 제작하였고 0.018×0.025인치 Stainless steel wire를 고정원 강화를 위해 상악 제 2소구치와 제 1대구치에 위치시켰다. 0.017×0.025인치 T-loop spring의 전후방적 위치에 따른 응력 분포를 알아보기 위해 T-loop spring의 중간이 견치와 제 1대구치 거리의 중간에 위치하는 것과, 2mm전방에 위치하는 것, 2mm후방에 위치하는 것의 3가지 유한 요소모델을 제작하였다. 이 T-loop spring을 6mm 강제변위시켜 발생하는 힘을 각 치아에 적용시켰으며, 견치에서 발생하는 힘과 응력에 대하여 다음과 같은 결론을 얻었다. 1. 3가지 형태 모두에서 유사한 크기의 후방견인력을 보였다. 2. 3가지 형태 모두에서 유사한 크기와 비슷한 형태의 응력 분포를 보였다. 3. 3가지 형태 모두에서 회전 중심은 치근단 2/3부분에 보였다. 4. 2mm 전방 위치된 T-loop spring과 2mm 후방 위치된 T-loop spring에 각각 함입력과 정출력을 보이지만 그 크기가 작아서 응력 양상에 영향을 주지 못한다. The purpose of this study was to find the difference of stress distribution on canine altered by the application point of preangulated T-loop spring. For this study, the finite element models of upper left canine, upper left second premolar and upper left first molar were made. Also, the finite element models of 0.017×0.025 inch preangulated, preactivated T-loop spring and 0.018×0.025 inch stainless steel wire were made. Three types of T-loop spring were made : the middle of activated T-loop is positioned in accordance with the middle position of instance of bracket position of both the canine and first molar, 2mm anterior, 2mm posterior. We compared the forces and the distribution of stress that were generated by difference of position of T-loop spring. The results were as follows. 1. All of the 3 types of T-loop spring showed the similar retraction forces. 2. All showed the similar amount & pattern of stress distribution. 3. The centers of rotation of canine in 3 types of T-loop spring were same and were positioned between C and D plane. 4. The canine showed the intrusive force by 2mm anterior positioned T-loop spring, but the extrusive force by 2mm posterior positioned T-loop spring. Neverthless, because of the small amount of the forces, the effect of vertical force was not significant.