Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

성장속도에 따른 대장균 rnpB 유전자 발현의 조절작용

전은순,이학란,이석재,정영환,조명선,정재훈,이영훈,박충웅 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Growth rate-dependent expression of the rnpB gene encoding M1 RNA, the RNA component of Escherichia coli RNase P, was examined using a hybrid transdcuing λ phage. The hybrid transducing λ phage was constructed carrying the lacZ gene fused to the promoter for the rnpB gene. The amounts of β-galactosidase were analyzed in the E. coli lysogen carrying this transducing phage grown in different growth media to see dependence of the rnpB transcription on growth rate. The synthesis of β-galactosidase from the rnpB-lacZ fusion increased with growth rate. This result suggests that the rnpB promoter activity is dependent on the growth rate.

네모나프라이드의 유효성 및 추체외로 부작용 : Haloperidol 및 Risperidone과의 자연관찰적 개방형 비교연구 A Naturalistic Open Comparative Study with Haloperidol and Risperidone

서영수,김용관,신동환,공보금,이정구,박정환,윤성환,정치영,이상경,김영훈 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.2

Objectives : This open prospective study was performed in order to evaluate the efficacy and extrapyramidal adverse effects of nemonapride in the schizophrenic patients, and was compared wit one of typical antipshchotics. haloperidol and one of atypical antipsychotics, risperidone. Methods : Thirty male and female schizophrenic patients(DSM-Ⅳ) were treated for 12 weeks with haloperidol(n=10), risperidone(n=10) and nemonapride(n=10). The overall efficacy was assessed at baseline, 1st week, 2nd week, 4th week, 8th week, and 12th week by the Positive and Negative Syndrome Scale for Schizophrenia(PANSS). Also the overall safety was assessed in the same time period by the Extrapyramidal Symptom Rating Scale(ESRS). Results : There were no significant differences in PANSS scores(total, positive, negative, and general psycho­pathology subscale) among haloperidol, risperidone, and nemonapride groups, Treatment responders, defined as at least 20% reduction of baseline total PANSS score, were achieved by 8 patients(80%) in haloperidol group, 10 patients(100%) in risperidone group, and 8 patients(80%) in nemonapride group. And there were no significant differences in ESRS total scores among haloperidol, risperidone, and nemonapride group. Inter-group comparison among haloperidol, risperidone and nemonapride group, as assessed by the ESRS, revealed no significant differences in the shifts to the maximum score, 9.4(±9.4), 6.2(±8.4), and 11.3(±8.1) respectively, and also revealed no significant differences in the mean time reaching the maximum score, 4.5(±4.5) week, 7.8(±4.8) week, and 4.6(±4.4) week, respectivily. Conclusion : There wee no significant differences in the efficacy and extrapyramidal adverse effects among haloperidal, rispecridone and nemonapride groups. These results suggest that nemonapride was as efficacious and safe in he treatment of schizophrenia, as well known and widely used antipsychotics, haloperidol and risperidone.

不眠에 對한 8-Chloro-6-Phenyl-4H-s-Triazolo[4.3-a][1·4]Benzodiazepine의 效果에 關한 臨床硏究

李定均,楊秉煥 大韓神經精神醫學會 1976 신경정신의학 Vol.15 No.4

In this study, authors examined the hypnotizing effect of Estazolam on patients in I.P.D & O.P.D. who visited with the chief complaint of insomnia. The number of patients selected for this study were 156. They were consisted of 78 neurotics other than depression, 66 depressive patients and 12 mild psychotic patients. Authors applied the double blind clinical method with Estazolam and placebo to the patients. They visited OPD & IPD of Psychiatric department of SNUH (Seoul National University Hospital) from October-1, 1975 to March-31, 1976. The results were as follows: 1. Estazolam had the highest sedating effect to the patients with mild psychosis and the least effect to the neurotics except depression. 2. Total duration of sleep increased approximately 20-30 minutes. 3. No difference was noticed between 2㎎ and 3㎎ administrated groups. 4. It seemed that Estazolam had no influencoe on REM sleep pattern (frequency of dream). 5. It was a most effective method to use Estazolam combined with other specific drugs to the patient who had suffered from insomnia for a long period. We applied the double blind clinical method with Estazolam and placebo to the patients who complained of insomnia. They were consisted of 78 neurotics other than depression, 66 depression patients and 12 mild psychosis. They visited OPD & IPD of psychiatric department of SNUH (Seoul National University Hospital) from October-1, 1975 to March-31, 1976. The result were as follows: 1. Estazolam had the highest sedating effect to the patients with mild psychosis and the least effect to the neurotics except depression. 2. Total duration of sleep increased approximately 20-30 minutes. 3. No difference was noticed between 2㎎ and 3㎎ administrated groups. 4. It seemed that Estazolam had no influence on REM sleep pattern (frequency of dream). 5. It was a most effective method to use Estazolam combined with other specific drugs to the patient who had suffered from insomnia for a long period.

열음향효과를 이용한 Lead Germanate 단결정의 상전이 연구

이수대,설정식,배세환 東亞大學校附設基礎科學硏究所 1993 基礎科學硏究論文集 Vol.10 No.1

Thermoacoustic signal which is gererated by the thermal waves have been measured from room temperature to 230°C. As a result, the intensity of thermoacoustic singal according to the variation of temperature is decreased remarkably at 177°C. Consequentely, the temperature of phase transition of the lead germanate single crystals is 177°C and it is equal to Curie point, and it must be the displacement ferroelectricity of the second order phase transition.

6-Sigma경영혁신기법 시행에 따른 방사선과 내부고객만족도의 변화

이근옥,박재성,강충환,김미영,김승식 한국의료QA학회 2005 한국의료질향상학회지 Vol.12 No.1

Background : The concept of 'service' in a hospital is based on the quality of medical staffs who serve as customer-satisfying media, and this is what distinguishes general hospitals from other business corporations in which customer satisfaction is achieved through commercial products. Thus, the internal employee satisfaction is essential in the improvement of the 'service' and subsequent 'competitiveness' of a hospital. The purpose of this study is to establish internal marketing strategies for a general hospital through regular surveys on employee satisfaction. Method : Surveys on employee satisfaction in radiology department were conducted regularly twice a year in a university hospital with 800 beds 2001 in 2004. The subjects of this study were 35 employees who work in radiology department. The authors developed the questionnaires and the surveys were conducted initially during the first 6 months of the year. After necessary improvements were made by applying 6 Sigma techniques, subsequent surveys were conducted during the latter 6 months of the year and the degree of employee satisfaction was compared. Results : Overall satisfaction increased with a constant rate and the authors were able to assess that the hospital is being gradually stabilized. The degree of satisfaction assessed by multiple choices showed only minor changes. However, as a result of focusing on the demands put forth by the employees through the open questions, the degree of satisfaction increased gradually year after year. Conclusions : It is important to heighten the internal employee satisfaction systematically and harmoniously through the assessment on the demands of the internal customers and feedback-based communications between the management officers and the staffs. Continuous surveys on the internal employee satisfaction will serve to be valuable materials in the establishment of internal marketing strategies for a general hospital.

열음향효과를 이용한 Lead Germanate 단결정의 상전이 연구

이수대,설정식,배세환 慶南大學校 附設 基礎科學硏究所 1993 硏究論文集 Vol.5 No.-

Thermoacoustic signal which is gererated by the thermal waves have been measured from room temperature to 230℃. As a result, the intensity of thermoacoustic singal according to the variation of temperature is decreased remarkably at 177℃. Consequentely, the temperature of phase transition of the lead germanate single crystals is 177℃ and it is equal to Curie point, and it must be the displacement ferroelectricity of the second order phase transition.

King Lear의 構造

李廷晥 全南大學校 人文科學硏究所 1992 용봉인문논총 Vol.21 No.-

〈Abstract〉 It is difficult to find a unified principle of construction of shakespeare's great tragedies because shakespeare chose different forms of construction in his tragedies which were thought to be suitable to express his idea of man's tragic destiny. This paper attempts to analyse the construction of King Lear in consideration of its theme and to prove that shakespeare chose the peculiar construction of King Lear to give expression to its theme.

아동행동 조사표에 의한 입원한 행동장애 청소년 분류

이환배,백기청,곽영숙 大韓神經精神醫學會 1992 신경정신의학 Vol.31 No.2

To make a suchclassification of conduct disorder by using CBCL. the authors investigated 37 conduct disorders adolescents in Seoul National Mental Hospital during the period from June 1, 1987 to June 30, 1991. The results were as follows : 1) The total numbers of conduct disorder adolescents are 37 patient, and 4 clusters are subclassified. 2) In one way ANOVA. the scores of externalizing and internalizing showed statistically significance. 3) To compare the score of subscale factor, in parent form CBCL, delinquent, aggressive, hyperactive factor showed statistically significance, and the remainder factors are not significant. 4) In comparison between clinical data and clusters, the age of symptom onset showed statistically significance, and orders are cluster 4, cluster 2, cluster 3 and cluster 1. The X2 test, the sex showed statistically significant the ratio of females 60.0% in cluster 2, 14.3% in cluster 3, 12.5% in cluster 1 and 0% in cluster 4. 5) i) In the main and interaction effects between 4 clusters and 2 forme of CBCL in each matched CBCL subscale, external, internal. aggressive(P<0.001) and deliniquent(P<0.01) factor showed statistically significant. ii) In the two forms of CBCL, social, school and total factor showed statistically significant. The remainder factor showed statistically no significance. iii) In the clusters and two forms of CBCL. the external and aggressive and internal factor showed statistically significance and in the cluster 1, the score of YSR of external and aggressive factor are higher than parent form CBCL, and in cluster 3 the scores of parent form and higher. In cluster 4, the score of internal factor of parent form CBCL is higher than the self form.

육군 범법자들에 대한 정신의학적 특성 분석

이규환,박흥수,이정태 大韓神經精神醫學會 1989 신경정신의학 Vol.28 No.2

The authors compared two hundred forty-eight imprisoned soldiers with two hundred seventy-nine normal controls using MMPl and Ego-Strength Scale in R.0.K. Army. The results were as follows ; 1) Mean age of imprisoned soldiers was 23.4, while that of controls was 23.9. 2) Educational level of imprisoned soldiers was lower than that of normal controls. 3) Pre-enlistment occupations in imprisoned soldiers chiefly were employees and factory workers (39.9%), while students consisted of 54.4% in normal controls. 4) More than half(61.4%) of the criminal acts had occured during the first year of the military service 5) Of the various criminal acts, absence without leave was the most frequent criminal activities(50.6%). 6) The result of MMPI was significantly different between two groups except L subscale. More specifically, F, Ma, Sc, Pd, Pa subscales which indicate a tendency of behavioral acting out were high in imprisoned soldiers, but all subscales showed below scores of 65. 7) Scores of Ego-Strength Scale in imprisoned soldiers were lower than that of the controls.