운동강도의 차이가 대장에서의 Heme oxygenase-1 발현에 미치는 영향: Oligonucleotide chip microarray analysis

최은주 ( Eun Ju Choi ),류호영 ( Ho Young Ryu ),차광석 ( Kwang Suk Cha ) 한국운동생리학회(구-한국운동과학회) 2012 운동과학 Vol.21 No.1

이 연구의 목적은 운동강도 차이에 의한 대장조직이 Heme oxygenase-1(HO-1)의 mRNA 발현에 어떠한 영향을 미치는지 규명하기 위해, Sprage-Dawley계 흰쥐를 대상으로 통제그룹(CON)과 저강도 운동그룹(LIE), 고강도 운동그룹(HIE)으로 구분하여 4주 동안 트레드밀운동 후 48시간 이후에 High through-put microarray analysis방법으로 다음과 같은 실험을 실시하였다. 본 실험은 Rat ABI oligo chip 26,857 개의 유전자 중 filtering을 통하여 12.079개 유전지를 선택하였고, 이 중 유의성 있는(p<.05) 유전자 12,072개를 선별하였으며, clustering분석 방법과 면역조직화학염색법에서도 HO-l이 운동강도에 따라 유의하게 발현하였다(p<.05). Microarray분석 후 RT-PCR로 확인한 결과 HO-I의 유전자 발현양상이 일치하는 결과를 얻었다. 이 실험의 결과로서 운동이 대장에서 HO-1 발현에 영향을 주는 것을 알 수 있었다. 운동이 대장에서의 mRNA 발현이 저강도 운동그룹(LIE)보다 고강도 운동그룹(HIE)에서 더 많이 발현되어지는 것으로 보아 고강도 운동에 의해 발생되는 산소라디칼을 HO-l의 유도를 통하여 적절히 제거함으로써 스스로 항상성을 유지하고 있는 것으로 생각되어진다. The purpose of this study is to identify how large intestine tissue effects to mRNA expression in Heme oxygenase-l (HO-1) due to differences in exercise intensity. To perform this experiment we divided Sprage-Dawley rats into 3 groups (control group (CON). low-intensity exercise group (LIE) and high-intensity exercise group (HIE)), and in 48 hours after 4 weeks treadmill exercise. the following experiment was performed with "High through-put micro array analysis methods" . 12,079 genes were chosen by filtering of the Rat ASI oligo chip 26.857 genes in this experiment Among 12.079 genes, we selected significant genes (p<.05) that were also expressed in the ways of clustering analysis and immunohistochemistry. With results of RT-PCR confirming and microarray analyzing, we derived that gene expression profiles of HO-l had been consistent. As a result of this experiment, we certainly identified that exercise effects to HQ-l expression in large intestine. mRNA expression was more expressed in high-intensity exercise group CHIE) than low-intensity exercise group (LIE), it seems that homeostasis maintains itself by properly removing oxygen radical. caused by high intensity exercise. through reduction of HO-l.

Arch교의 橫桁連結部 局部應力과 疲勞擧動에 관한 硏究

최광순,조호근,손영호,최광규 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

In this paper, the relation between the local stress and the fatigue behavior at gusset plate connected with main girder and floor beam in arch bridges was obtained. The conclusion was as follows : (1) In-plane bending moment and out-of-plane bending moment are higher than other internal stress resultants. (2) When a vehicle crosses the bridges, the change of out-of-plane bending moment corresponds with the change of relative difference in displacement in the longitudinal direction between the tie-girder and stringer. (3) The change of in-plane bending moment corresponds with the change of the displacement in the transverse direction of arch-rib. (4) Fatigue crack can be initiated by in-plane bending stress that Is produced by out-of-plane bending moment at the toe of gusset plate. (5) Fatigue crack can be initiated by tension stress that is produced by in-plane bending moment at the toe of gusset plate.

Arch교의 橫桁連結部 局部應力과 疲勞擧動에 관한 硏究

최광순,조호근,손영호,최광규 東亞大學校 建設技術硏究所 1997 硏究論文集 Vol.21 No.2

In this paper, the relation between the local stress and the fatigue behavior at gusset plate connected with main girder and floor beam in arch bridges was obtained. The conclusion was as follows : (1) In-plane bending moment and out-of-plane bending moment are higher than other internal stress resultants. (2) When a vehicle crosses the bridges, the change of out-of-plane bending moment corresponds with the change of relative difference in displacement in the longitudinal direction between the tie-girder and stringer. (3) The change of in-plane bending moment corresponds with the change of the displacement in the transverse direction of arch-rib. (4) Fatigue crack can be initiated by in-plane bending stress that is produced by out-of-plane bending moment at the toe of gusset plate. (5) Fatigue crack can be initiated by tension stress that is produced by in-plane bending moment at the toe of gusset plate.

Azithromycin으로 치료한 임신 중 쓰쓰가무시병 1예

김광석,최진욱,서호종,김기훈,박성호,서광섭,고성만,김순혜,김호정 대한감염학회 2001 감염 Vol.33 No.5

We report a case of tsutsugamushi disease in a 26 year-old pregnant woman who was treated with azithromycin. Her gestation period was 27 weeks and she admitted with fever, rash, and eschar on the right shoulder. Currently recommended medications for the treatment of scrub typhus are doxycycline or chloramphenicol. But, these drugs are class D drugs according to the U.S. Food and Drug Administration (FDA) Fetal Risk Summary, so they couldn't be used to treat pregnant women. Recently, a few case reports suggested that azithromycin, a relatively new macrolide antibiotic, was effective and safe for the treatment of scrub typhus in pregnant women. And, there is no evidence that azithromycin causes harmful effects to the developing fetus or to children. On the basis of current in vivo test that confirms the effectiveness of azithromycin, it may be the drug of choice for the treatment of scrub typhus in pregnant women and children. (Korean J Infect Dis 33:380-382, 2001)

국산 Lidocaine 알칼리화를 위한 Bicarbonate 혼합시 침전유발 최소량에 대한 고찰

이선호,김현수,곽인숙,안원식,최관호,김광민,손민제 대한마취과학회 2000 Korean Journal of Anesthesiology Vol.39 No.5

Background : When local anesthetics for regional anesthesia is used, usually small amounts of bicar-bonate are added for rapid onset. This addition gives the mixed solution a more alkaline pH. The following result is an increased unionized form of the local anesthetic and rapid penetration of the drug into tiissue. Unfortunately, no data about adequate mixing volumes of domestic lidocaine and bicarbonate is available. Methods : We examined six mixing pairs of two kinds of 2% lidocaines and three kinds of 8.4% bicarbonates for minimum volumes of bicarbonate to cause a precipitation of 2% 20 ml lidocaine. Results : The mean volumes of bicarbonate to cause precipitation were 1.54 ml for Kwang-Myung lidocaine with Kwang-Myung bicarbonate, 2.90 ml for Kwang-Myung lidocaine with Dae-Won bicarbonate, 2.73 ml for Kwang-Myung lidocaine with Je-Il bicarbonate, 0.97 ml for Je-Il lidocaine with Kwang-Myung bicarbonate, 1.26ml for Je-Il lidocaine with Dae-Won bicarbonate and 1.39 ml for Je-Il lidocaine with Je-Il bicarbonate. Conclusions : We conclude that the Kwang-Myung lidocaine and the Je-Il lidocaine could cause precipitation when mixing with a smaller bicarbonate volume than foreign textbook recommended. (Korean J Anesthesiol 2000; 39: 726-729)

고속 가공기의 스핀들 진동에 영향을 주는 요소 분석

최영호,윤두표,김광영,최병오 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

In this paper, We have studied on the critical vibration limits of spindle unit for the high speed ball pen tip processing machine. The vibration of bearing can be measured by FFT, and the influence of vibration amplitude due to the Unbalance, bearing deflect, bite and timing belts tension are analyzed. So, the critical vibration limits of spindle is determined by the X, Z directional vibration of spindle Unit.

완전탈구된 치아의 재식에 관한 치험례

최영철,권오연,김광철,이긍호 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.2

Exarticulation includes all cases in which the tooth has been totally displaced out of its socket. The main etilogies in the permanent dentition are direct blows and traffic accident, while in the primary dentition are fall-downs. The prognosis of tooth which has been recieved endodontic treatment after replantation is better than received endodontic treatment before replantation. Ca(OH)₂as a initial canal filling material diminishes undesirable replacement root resorption, so in the cases of compelted and uncompleted root formation, the pulp is extirpated and then a Ca(OH)₂dressing is placed until optimal stage for final canal filling with gutta percha cones. Storage media and storage period of avulsed tooth is very important things for prognosis after replantation. A strong relationship has been found between dry storage or storage in non physiologic media (c.g tapwater) and root resorption. Physiologic saline and/or fresh milk is one of the best storage media.

ZnS 완충층을 사용한 SrS:Ce, Cl 박막 EL소자의 발광특성

최광호 남서울대학교 2005 남서울대학교 논문집 Vol.11 No.2

Luminescence characteristics of SrS:Ce,Cl TFEL devices with ZnS buffer and double insulating layers has been investigated. The density of transferred charge is gradually increased along with the Applied voltage up to 200V and that it is sharply increased along with the applied voltage above 200V. This phenomenon is thought to that the density within shallow trap is relatively low and that it within deep trap is high and distributed wide. The crystallinity of a SrS:Ce,Cl active layer is strongly affected by the underlayer. Bright blue emission is achieved in a thin film EL device having a strongly (111) oriented ZnS film as its underlayer.

主要作物의 微量要素에 關한 硏究 : 第1報 硼素 및 有機物이 배추 生育에 미치는 影響 1. Effects of boron and organic matter on the chinese cabbage

崔元凱,河浩成,鄭鎬權,姜鎬允 진주산업대학교 1969 論文集 Vol.3 No.-

硼素缺乏地帶의 배추圃場에서 硼素의 施用方法 및 施用量을 달리하여 얻은 收量으로 肥效을 追求하고, 또 完熟堆肥의 效果를 調査한 結果를 要約하면 다음과 같다. 1. 硼砂 土壤施用에 依한 배추의 收量은 分散分析結果 1% 水準의 高度의 有意性을 보였으며, 硼砂 10a當 1,500㎏施用의 것이 가장 많은 收量이었고, 그 以上의 硼砂施用에서는 오히려 減少했다. 2. 배추의 硼酸 葉面施用의 差異에 依한 收量差는 土壤施用 때처럼 뚜렷한 差를 볼 수 없었다. 大體로 硼酸 0.25%∼0.5%의 濃度로 葉面撒布한 것이 좋은 結果를 거두었다. 3. 完熟堆肥의 增施에 따라 배추의 收量은 增加되었다. 10a當 1,800㎏ 施用에서 最大値의 收量에 이르고 그 以上의 增施에서는 별다른 收量의 增加를 볼 수 없었다. This experiment was designed to make clear the effects of the application of Boron and organic matter on chinese cabbage growing in Boron dificient soil. The results obtained were as follows. 1. The application of Borax in increased remarkably the yield of the chinese cabbage. The yield was increased by the application of Borax in the soil. It reached maximum amounts at 1,500g per 10a, but decreased above 1,500g per 10a. 2. The various concentration of Boron on the leaf spray was no more effective than the application of Boron in soil. The adequate concentration of Boric Acid on the leaf spray was from 0.25 percent solution to 0.5 percent solution. 3. The yield of the chinese cabbage was increased by the application of the compeletely decayed compost. It reached maximum amounts at the application if 1,800㎏ per 10a, but there was no effects above 1,800㎏ per 10a.

압자의 압입에 의한 치관 전장용 복합레진의 기계적 성질 평가

최원호,배태성,박광선,박종수 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.2

This study was performed to evaluate the mechanical properties of four commercially available veneering resin composites for crown (Dentacolor: DC, Artglass: AG, Esternia: ET and Targis: TG). According to the manufacturer's instructions, disc specimens were fabricated in a teflon mold giving 12mm in diameter and 1 mm in thickness. All specimens were immersed in distilled water at 37℃ for 30 days. An indentation test of 68-degree trigonal diamond pyramid was made under 98.06 mN load for 15 seconds. Hardness numbers, charateristic indentation depths, flow and permanent deformation were measured and compared statistically by ANOVA and Tukey's multiple range test. The results obtained were summarized as follows; 1.The highest hardness value of 87.4 was observed in the ET group and the lowest value of 11.8 was observed in the DC group. Hadness values showed no sinificant difference between the AG group and TG group, but others showed the significant difference beween groups (p<0.5). 2.Flow value of the ET group was significantly lower than those of the AG and TG groups of hybrid composite for crowns. 3.Percent strain during the indentation was the lowest in the DC group and the highest in the AG group, but there was no significant difference in each experimental group (p>0.05). 4.The relationship between the hardness number and flow during the indentation was a negative linear on a logarithmic scale.