집토끼의 시상하부내에서 Vasopressi과 Oxytocin 분비세포의 미세구조에 대한 면역세포화학적 연구

김종중,김홍중,김현곤 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

An immunocytochemical study was performed using the specific monoclonal antibodies against arginine vasopressin (VP) and oxytocin(OT) to demonstrate vasopressinergic and oxytocinergic neurons in the hypothalamus of the domestic rabbit. 1. VP and OT neurons were distributed mainly in the supraoptic and paraventricular nuclei. 2. The VP and OT neurons were round, oval, spindle and polygonal in shape, and the diameters of the neurons were 15-25um in domestic rabbit. 3. VP neurons were dominant in the supraoptic nucleus and OT neurons were in the paraventricular nucleus. 4. From the electron microscopic observations, the rough endoplasmic reticulum, mitochondna, Golgi apparatus and microtubules were found in the VP and OT neurons.

흰쥐 척수 손상후 Parvalbumin과 Calbindin D-28K 함유신경세포에 관한 면역세포화학적 연구

김종중,정윤영,임유택,박상수,박영란,김현곤,문정석 조선대학교 부설 의학연구소 2002 The Medical Journal of Chosun University Vol.27 No.2

Background and Objectives: This study was done to compared the distribution of the two calcium-binding proteins immunoreactive neurons, parvalbumin immunoreactiv (PV-IR) and calbindin D-28K immunoreactive (CB-IR) neurons in the spinal cord after transection. Materials and Methods: Twenty rats were divided into 3 groups (control, complete spinal cord injury (SCI), and right and left spinal cord hemisection). SCI was produced by cutting the spinal cord use blades 11 with scalpel handles. Results: In this experiment, CB-IR neurons were mainly found in many pyramidal cells distributed in the brain stem and spinal cord of rats. PV-IR Neurons were demonstrated in all lamina of the gray matter of the spinal cord. These immunoreactive cells had the highest density in the layer I and II of dorsal horn and several nuclei of the ventral horn of the all the segments of the spinal cord. CB-IR neuropil labeling was strongly noted in all the segments of the spinal cord. In contrast PV-IR neurons were different in distribution, size and morphology in the spinal cord. The number of PV-IR neurons were greater than in the spinal cord compared with the CB-IR neurons. CB-IR and PV-IR somata were round, oval, spindle and polygonal in shape, and were unipolar, bipolar, multipolar and horizontal in types. The diameters of the somata of the PV-IR and CB-IR neurons were 40-50 ㎛, respectively. Also dendrites of PV-IR and CB-IR neurons were densely arrayed in network.

잭슨 구조적 프로그래밍 방법의 통합을 위한 새로운 객체지향 방법에 관한 연구

정현숙,조정은,김행곤 대구효성가톨릭대학교 응용과학연구소 1995 응용과학연구논문집 Vol.4 No.-

Software is on the increase to translate existing system into an object-oriented system that is due to spread a problem recognition to have structured development methodology and prosperity of object-oriented related techniques. This paper presents a new object-oriented method which integrates the Jackson Structur-ed Programming method with an object-oriented approach. The new method analyzes the user's services, identifies attributes from data structure diagrams, and identifies operations from program structure diagrams. As well, this method defers the identification of class relationship until the attributes and operations have been identified, so as to present premature desicions. Besides, the con-cepts of "leading object" is used to decrease the number of actors and agents and to in-crease the number of servers in a system. Some of the results may also enhance reusability of the objects.

식물체로부터 추출한 물질이 상추 종자의 발아 및 생장에 미치는 영향

박건남,이민경,황선주,김학윤,이인중,신동현,김길웅 경북대학교 농업과학기술연구소 1999 慶北大農學誌 Vol.17 No.-

This study was conducted to detect the allelopathic effect of water and ethanol extracts from 4 plant species (Lactuca sativa L., Ambrosia elatior L., Oenothenra odorata Jasp., Ginkgo biloba L.) on germination and growth of lettuce seeds. A little bit higher inhibitory effect was obtained in ethanol extracts rather than water extracts. Allelopathic effects varied in the source of extracts and concentrations of 4 plant species and the highest allelopathic effect appeared in 10% solution, regardless of plant species.

사람의 비후성 반흔에서 면역조직화학기법에 의한 형질전환인자 알파와 표피성장인자 발현의 차이에 관한 면역조직화학적 연구

박영란,김현곤,김종중,문정석,송준섭,설경수,정윤영 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

Background and Objectives: Transforming growth factor-α (TGF-α) and epidermal growth factor (EGF) are polypeptides which interact with the epidermal growth factor receptor (EGFR) to produce their biological effects. The aim of the present investigation was to elucidate the immunolocalization of TGF-α and EGF in normal human skin, hypertrophic scar with skin graft on soft tissue defect, and postburn hypertrophic scar without graft. Methods: The data presented in this paper focused attention on differences of expression between two kinds of hypertrophic scars in relation to skin graft using immunohistochemistry. Formalin-fixed and paraffin-embedded tissues from 3 normal skin tissues, 3 hypertrophic scars after skin graft and 8 postburn hypertrophic scars without graft were immunolabelled with antibodies directed against TGF-α and EGF. Results: In normal epidermis of skin, strong TGF-α immunoreactivity (IR) was observed in all epidermal layers except the stratum (S.) basale, whereas EGF was immunopositive in a few cells over all epidermal layers. The staining for TGF-α was found in cell membrane, and EGF was seen either diffuse cytoplasmic or peripheral part of cells in normal epidermis. In hypertrophic scar after six months postoperation, TGF-α IR was observed in the upper part of S. spinosum and S. corneum, whereas EGF was diffusely expressed in the S. spinosum, also its intensity was slightly increased compared to normal skin. In hypertrophic scar without skin graft, very strong expression of EGF was detected in the S. spinosum and S. corneum, and intensity of EGF was increased when compared to normal skin and postoperative hypertrophic scar. EGF and TGF-α coexisted in the cells of S. spinosum in normal skin as well as hypertrophic scars. Double-labeled cells were increased in postburn hypertrophic scar compared to normal skin and postoperative hypertrophic scar. In addition, intensity of EGF and TGF-α double IR was the strongest in postburn hypertrophic scar. Conclusion: These results indicate that EGF may have a more complex regulatory role not only in the early stages of wound healing but also in hypertrophic scar.

흰쥐 편도체에서 칼슘결합단백인 calbindin-D28K함유 신경세포의 생후 발달에 관한 면역조직학적 연구

정윤영,김종중,문정석,박영란,정상봉,김현곤,박상수 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Background and Objectives : The calcium-binding protein, calbindin-D28K(CB) is widely distribution of CB in rat amygdala during postnatal maturation, in order to provide an insight into the developmental role it may play in the amygdaloid neurons. Materials and Methods : The present study is based on 28 postnatal cases ranging from the day of birth, postnatal day 0 (P0) to 30 days (P5, P10, P15, P20, P30), and these cases were compared with adult rat amygdala. Cryostat sections processed free-floating with monoclonal antibody by immunohistochemistry. Results : CB-immunoreactive neurons were already found at birth in all amygdaloid nuclei except the intercalated nucleus. The intensity and number of immunoreactivity of CB-containing neurons increased during the first 10 days of postnatal life but dramatically decreased at P15. Mature patterns of CB-immunoreactive neurons were achieved at P20. In the adult amygdala, CB-immunoreactive neurons were distributed in almost all amygdaloid nuclei except the intercalated nucleus. Conclusion : The present results show early appearance of CB immunoreactivity in the amygdala. It may be related to the early appearance of CB immunoreactivity in many other brain regions, and suggests that CB may play a functional role during the development of the nervous system including amygdala.

여자고등학교 한국무용수의 춤별 운동강도

全鍾貴,金一坤,金顯美 忠南大學校體育科學硏究所 1992 體育科學硏究誌 Vol.10 No.1

The purpose of this study is to compare the heart rate of Korean dancers who have been trained 6 hours a week for three years with that of general girl students who have never been experienced as an athlete and participated exercise programs regularly. We have studied the change of heart rate until the subject could no longer continue the treadmill exercise by the added velocity every 5 minutes. The following conclusions were obtained. 1. Observation of heart rate about regularity exercise intensity. 1) There was no difference of the heart rate between two groups during the first 5 minutes(3 mph) and 10 minutes(4 mph) of exercise. (p〉.05) 2) The difference of exercise intensity between two groups began to exhibited during 15 minutes (5 mph) of exercise. (p〈.001) 3) During the high-intensity exercise for 20 minutes (6 mph) and 25 minutes (7 mph), the difference of heart rate between two groups was low, but there was high in difference. (p〈.05) 4) The difference of heart rate between two groups in all-out condition for 30 minutes (8 mph) of exercise revealed that the motor skill of general group was superior to that of korean group. (p〈.001) 5) The heart rate recovery of general group was faster than that of korean dance following exercise. 2. Observation of exercise intensity in staging korean dance. 1) The exercise intensity sort of dances were Hwa Kwan Mu 50.4%, Sung Mu 55. 3%, Bu Ch'ae Ch'um 60.2%, O Buk Ch'um 66.7%, Kum Mu 68.3% and Mu Dang Ch'um 89%. 2) The maximum exercise intensity was 89% as a Mu Dang Ch'um. 3) The mean exercise intensity provided by general group for three years constantly was 65%.

기니이픽 장관의 c-Kit 및 NK 1R 면역반응 세포구조에 대한 공초점 주사현미경적 연구

장인엽,김종중,문정석,김현곤,박찬국,전제열,전규배,조철희,유호진 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Background and Objectives: Immunolabelling of interstitial Cajal(IC) cells in the intestinal wall has recently been developed by using a specific marker, the anti-c-Kit antibody. Substance-P is a well-known neurotransmitter in the gastro-intestinal tract. Since the gastro-intestinal wall structures have already been well documented in the guinea pig, immunohistochemistry was done for the c-Kit-positive IC network and substance-P receptor(NK1R) in an attempt to provide a morphological basis for the mechanism regulating gastro-intestinal movement. Materials and Methods: Cryosection and whole-mount preparations of guinea pig small intestine and colon were single and double immunolabelled using the anti-c-Kit and NK1R antibodies. Immunolabelled specimens were observed under a confocal laser scanning microscope. Results : According to a three dimensional reconstruction study, it was found that (1) the c-Kit-positive celluar networks were widely distributed in the intestinal wall, (2) c-Kit-positive celluar networks encircled the ganlion, with strands in reticular configurations, and (3) the c-Kit-positive cells showed colocalization with NK1R in circular muscle(CM), not myenteric plexus(MY). Conclusion: The charateristic profiles of IC containing c-Kit-positive celluar networks and the relationship between c-Kit-positive and NK1R-positive structures provide a morphological basis upon the mechanism regulating gastro-intestinal motility.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

Aminopeptidase M 저해제인 Valistatin과 des-Asp^4-Amastatin을 생산하는 방선균 SL20209의 특성 및 동정

고학룡,전효곤,정명철,서현효,김홍중,박용하,고영희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

Aminopeptidase M 저해제인 valistatin과 des-asp^4-amastatin을 생산하는 방선균 분리주 SL20209의 특성을 조사하고 수리동정을 실시하였다. 형태적, 배양학적 및 생리적 특성으로 부터 SL20209는 방성균 중에서도 Streptomyces 속에 속하는 것으로 나타났으며, 43개의 분류단위 특성으로 TAXON program을 이용하여 수리동정한 결과, 주군집 29의 Streptomyces 중 Streptomyces griseoplanus와 가장 근접한 것으로 나타났다. 따라서, 분리주 SL20209를 S. griseoplanus SL20209로 동정하였다. Characterization and numerical identification were carried out for an actionmycetes SL20209. Morphological, cultural and physiological properties of SL20209 which produced valistatin and des-asp^4-amastatin as inhibitors of aminopeptidase M were evaluated. The isolate was identified to be the genus of Streptomyces. Fourty-three taxonomic units were analysed by using a TAXON program. The isolate was classified into the major cluster 29 of Streptomyces and best-matched to Streptomyces griseoplanus.