Neuroprotective effects of bovine colostrum on intracerebral hemorrhage-induced apoptotic neuronal cell death in rats

Kim, Sung Eun,Ko, Il Gyu,Shin, Mal Soon,Kim, Chang Ju,Ko, Young Gwan,Cho, Hanjin Medknow PublicationsMedia Pvt Ltd 2012 Neural regeneration research Vol.7 No.22

<P>Brain cell death after intracerebral hemorrhage may be mediated in part by an apoptotic mechanism. Colostrum is the first milk produced by mammals for their young. It plays an important role in protection and development by providing various antibodies, growth factors and nutrients, and has been used for various diseases in many countries. In the present study, we investigated the anti-apoptotic effects of bovine colostrum using organotypic hippocampal slice cultures and an intracerebral hemorrhage animal model. We performed densitometric measurements of propidium iodide uptake, a step-down avoidance task, Nissl staining, and caspase-3 immunohistochemistry. The present results revealed that colostrum treatment significantly suppressed N-methyl-D-aspartic acid-induced neuronal cell death in the rat hippocampus. Moreover, colostrum treatment improved short-term memory by suppressing hemorrhage-induced apoptotic neuronal cell death and decreasing the volume of the lesion induced by intracerebral hemorrhage in the rat hippocampus. These results suggest that colostrum may have a beneficial role in recovering brain function following hemorrhagic stroke by suppressing apoptotic cell death.</P>

일측성 질폐쇄 및 동측 신장 무발생을 동반한 중복자궁 2예

윤일영,여소진,김형문,김정식,김태희,이해혁,남계현,이권해,김준모,김민의 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Uterus didelphys with unilateral obstructed hemivagina and ipsilateral renal agenesis usually presents after menarch with progressive abdominal pain during menses secondary to hematocolpos, and indeed a very rare congenital anomaly due to Mullerian duct malformation. An accurate and prompt diagnosis is of importance to permit treatment and to assure the future fertility of the patient. Pelvic ultrasound, physical examination and pelvic MRI established a diagnosis of hematometracolpos secondary to uterus didelphys with unilateral imperforate hemivagina. An incision in the vaginal septum allowed drainage of the hematocolpos, providing relief of the patients symptoms. We report two case of uterus didelphys with obstructed hemivagina with brief review of the literature.

제4형 가족성 고중성지방혈증 임산부에서 발생한 급성 췌장염 1례

박관응,윤채중,김영학,진영기,윤성호,권용은,김태원,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.2

Plasma levels of cholesterol (TC) and triglyceride(TG) physiologically increase during pregnancy. The lipid increment is 23-53% above the pregravidic level for TC and 2- to 3-fold of the prepregnancy level for TG. If the TC and TG are higher than normal values in pregnancy, the patient must be carefully monitored. Acute pancreatitis is the main consequence of hyperlipidemia and occurs either during pregnancy, in the third trimester, or in the puerperium. Mortality is high both for the mother (21%) and the fetus (20%). We report a case of 28 year-old pregnant woman at 29 weeks gestation with hypercholesterolemia (TC = 357 ㎎/dl) and severe hypertriglyceridemia (TG = 1300 ㎎/dl). The patient was admitted to the hospital because of severe epigastric pain, nausea and vomiting. Total serum cholesterol was increased and trigyleride was markedly increased. The electroporesis pattern of serum lipoprotein showed increase in pre-beta lipoprotein fraction, suggesting IV hypertriglyceridemia pattern. According to a review of the literature, the incidence of pancreatitis during pregnancy is 1 in 1000 to 3000 pregnancies. Severe hypertriglyceridemia in pregnancy should be treated with a careful restriction of calories and fat: for preventing acute pancreatitis, hospitalization for intravenous fluid therapy and plasma exchange must be required.

미치광이풀 모상근 배양에서 Tropane Alkaloid 생산성 증진을 위한 최적 생물학적 엘리시터 선발

정희영,강승미,강영민,김용덕,양재경,정영관,최명석 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Scopolamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supematant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.

혈소판 풍부혈장의 임상적 응용

하정완,김수관,조세인,이철우,정태영,김수흥,김영균 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.5

Platelet-rich plasma is an autologous source of platelet-derived growth factor and transforming growth factor beta that is obtained by sequestering and concentrating platelets by gradient density centrifugation. We have used platelet-rich plasma for bone graft, especially allobone graft, at implant surgery, sinus lift procedure, and cyst enucleation. This article is retrospective study from October 1999 to November 2000. All cases were good healing and clinical success.

H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

메인 메모리 DBMS 데이터의 안정적 저장 기술 분석

김영관,박현주 한밭대학교 정보통신전문대학원 2003 정보통신전문대학원 논문집 Vol.1 No.1

반도체 메모리 기술의 발전은 메인 메모리 데이터베이스가 사용 될 수 있는 기반이 되었다. 메인 메모리데이터베이스는 디스크 기반 데이터베이스에 비해 빠른 접근 시간과 균일한 성능 특성을 가진다. 메인 메모리 데이터베이스는 메인 메모리에 최적화된 데이터베이스 구조를 설계하고 관리하는 기법에 따라 성능상의 차이가 발생한다. 본 논문에서는 상용 메인 메모리 데이터베이스에서 데이터를 안정적으로 저장하는 기술들에 대해 기술 한다. Development of semiconductor memory technology became a cornerstone that MMDB(main memory database) can be used MMDB has higher access time and more equable performance property than disk resident database. MMDBs have differences of performance according to design methods of MMDB structure. This paper describes technologies that store data stably in commercial MMDB.

Pilot plant와 Jar test를 이용한 응집제 LAS와 PAC의 수처리 효과 비교

김운중,고창옥,조영관 朝鮮大學校 建設技術硏究所 2001 建設技術硏究 Vol.21 No.1

Juam raw water was used to investigated water treatment efficiency in terms of coagulants and experiment types as jar-test and lab-scale pilot plant. pH of treated water was about 0.1~0.2 high when pilot plant was used than when jar-test was used in all of coagulants as liquid aluminium sulfate(LAS) and poly aluminium chloride(PAC). In comparing pH of surface water between in actual precipitation process with in pilot plant precipitation process when LAS was used, variation range of pH was 0.16 in pilot plant process whereas 0.04 in actual process. In comparing color, turbidity, zeta-potential, aluminium of precipitated surface water, it showed same results in all of coagulants as PAC and LAS and all of processes. In comparing alkalinity of the water, PAC showed alkalinity was 2∼3ppm higher than when LAS was used because of coagulants' characteristics as Al2O3 contents. Langarier indices as Corrosive index were -2.95 in LAS and -2.66 in PAG, which means PAC has less corrosive than LAS. In, comparing the removal of algae using LAS and PAC in jar-test and pilot plant, the removal rates were 80% in jar-test and 75% in pilot plant. The rate was improved to 90% after when incline baffles were used. Therefore, PAC is appropriate in considering prevention of corrosion, on the other side, LAS is appropriate in considering economy.

모바일시스템을 위한 다중 체크포인트 플래시 파일시스템

김영관,박현주 한밭대학교 정보통신전문대학원 2004 정보통신전문대학원 논문집 Vol.2 No.1

플래시 메모리는 전력소모가 낮고 부피가 작으며 메모리처럼 빠른 접근 속도를 가지지만, 기록을 위해 미리 삭제하는 과정을 거쳐야 하며 그 횟수가 제한되는 단점이 있다. 본 논문에서는 다중-체크포인트 정보를 이용 하여 대용량의 플래시 파일시스템을 효율적으로 관리하기 위한 MJFFS를 제시한다. MJFFS는 JFFS의 로그 기반 파일시스템의 장점과 JFFS의 단점을 극복한 빠른 마운트와 오류회복 수행으로 모바일 기기에 적합할 것이다. The flash memory consumes low power, is a small size, and has a fast access time like the main memory. But the flash memory must erase for recording and the erase cycle is limited. This paper proposes a MIFFS to using a multi-checkpoint information to manage a mass flash filesystem efficiently. A MIFFS will be suitable ato a mobile device owing to accomplish fast mounting and error recovery using advantage of log foundation filesystem and overcoming defect of JFFS.