토양처리용 천연물제초제 개발을 위한 생물검정법 개선

김재덕(Jae Deong Kim),황현진(Hyun Jin Hwang),서보람(Bo Ram Seo),최정섭(Jung Sup Choi),김진석(Jin Seog Kim) 韓國雜草學會 2011 Weed&Turfgrass Science Vol.31 No.3

본 연구는 토양처리용 천연물 제초제의 개발을 보다 신속하게 효율적으로 진행하기 위한 방안으로서 실용적인 입장에서 보다 개선된 검정법을 확립하기 위해 수행되었다. 검정하고자 하는 화합물의 량이 50㎎ 이상 충분할 경우, 잡초 4초종(피, 바랭이, 자귀풀, 어저귀)이 파종된 50㎠ 토양 표면적을 가진 폿트에서 온실검정을 함으로서 기존의 방법(토양 표면적이 350㎠인 폿트 이용)에 비해 약제 소요량을 78배 줄일 수 있었다. 이때 검정초기 처리농도는 10,000㎍ mL<;SUP>;-1<;/SUP>; 내외로 하는 것이 적당하였다. 한편 검정용 시료의 량이 10㎎ 이하로서 미량일 경우는 6 well plate를 이용하되 각 well에 밭토양을 담고 4가지 초종을 파종한 다음 실내에서 제초활성을 검정하는 방법이 추천될 수 있었다. 이는 기존의 방법(토양 표면적이 350㎠인 폿트 이용)에 비해 약제 소요량 및 처리용액 부피를 14배 가까이 줄일 수 있었고 실제에 가까운 데이터를 확보할 수 있어 보다 빠른 속도로 천연물 제초제를 개발하는데 도움이 될 것으로 판단되었다. This study was carried out to establish an improved bioassay system on the side of practicality, pre-emergence bioassay which is more effective in developing soil application natural herbicides. A miniaturized method which have a 50 cm² of soil surface area and was efficient by 7 times compared to the existing soil application assay (350 cm² of soil surface area) was established, in which four weed species (Echinochloa crus-galli, Digitaria sanguinalis, Aeschynomene indica, and Abutilon theophrasti) were planted and grown in greenhouse. This would be applicable when the amount of screening compound is much more than 50 mg. The initial application rate was desirable at 10,000 μg mL-1. On the other hand, the 6 well plate assay which has 4 weed species in each well containing upland soil and could be conducted in growth chamber, was established. This assay was resulted in minimizing in level of 1/14 test volume and 1/14 amounts of test compound to the conventional method that has been used for screening of synthetic compounds in KRICT, and applicable for the small amount of test compound (less than 10 mg). Therefore, the improved bioassays established in this study would be helpful for a rapid and efficient development of soil application natural herbicides.

BCR-ABL 융합유전자를 동반한 급성 골수구성 백혈병 1예

최정민,송성은,이규진,이규원,이호섭 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

BCR-ABL 융합 유전자를 동반한 급성골수구성백혈병은 그 빈도가 매우 낮고, 만성골수구성백혈병의 급성악화기와 임상적으로 유사한 특징을 가진다. 항암화학요법으로 관해유도가 되기 어렵거나, 관해유도 후 재발의 빈도가 높은 특징을 가지고 있어 예후가 나쁘다고 알려져있다. 저자들은 BCR-ABL 융합 유전자를 동반한 급성골 수구성백혈병1례를 경험하였기에 문헌고찰과 함께 보고 하는 바이다. BCR-ABL fusion gene is the hallmark of chronic myelogenous leukemia (CML), but it has been rarely reported in acute myeloid leukemia (AML). AML with BCR-ABL fusion gene has different features from blast crisis of CML. We experienced a rare case of AML with BCR-ABL fusion gene. Here we report this case with a brief review of literatures.

박막 크로멜-알루멜 열전퇴형 교류-직류 변환기

정인식,김호운,김진섭,이정희,이종현,신장규,박세일,권성원 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

A planar chromel-alumel multijunction ac-dc converter was fabricated on a LPCVD Si_(3)N_(4)/SiO_(2)/Si_(3)N_(4)-diaphragm, prepared by silicon bulk micromachining, which thermally isolated a bifilar evanohm-heater and the hot junctions of a chromel-alumel thermopile from the silicon substrate. The voltage responsivity, the ac-dc transfer error, and the fluctuation of the output thermoelectric voltage of the converter were investigated. The respective voltage responsivities in air and in a vacuum of the converter were about 3.16 mV/mW and 9.41 mV/mW. The ac-dc voltage and the current transfer errors in air were about ±1.5 ppm and ±0.7 ppm in a frequency range from 10 Hz to 10 kHz, respectively. The fluctuation of the output thermoelectric voltage from 5 seconds to 120 seconds after preheating in air for 5 seconds was about 0.06 % for a heater input of dc 1 V.

도메인이름 이전청구권 부존재확인청구소송에 관한 판례의 비판적 고찰

정진섭,김동균,최재원 法務部 商事法務課 2009 선진상사법률연구 Vol.- No.46

ccTLD 즉 '.kr' 도메인이름의 등록과 사용에 관련된 분쟁의 조정을 원하는 자는 인터넷주소분쟁조정위원회의 도메인이름분쟁조정규정에 의하여 조정위원회에 조정을 신청할 수 있으며, gTLDs 즉 '.com' 도메인이름의 등록과 사용에 관련된 분쟁의 해결을 원하는 자는 ICANN의 공인을 받은 WIPO 중재센터 등의 조정결정을 신청할 수 있다. 도메인이름 등록자는 등록대행기관을 통해 도메인이름을 등록할 때 이러한 강제 조정 내용을 담은 약관에 동의하도록 되어 있다. 통상 상표권자는 도메인이름 등록자가 등록약관에 동의한 것에 기초하여 gTLDs에 대해서는 ICANN 공인 분쟁조정기관에, ccTLD인 '.kr' 에 대해서는 인터넷주소분쟁조정위원회에 분쟁대상 도메인이름에 관한 분쟁조정을 신청하고, 이 조정 절차에서 WIPO중재센터 혹은 인터넷조정위원회는 분쟁 대상 도메인이름들의 등록을 말소하라고 결정하고 있다. 물론 도메인이름 등록자는 위의 조정부 결정과 관련하여 gTLDs의 경우엔 UDRP, ccTLD인 '.kr' 에 대해서는 도메인이름분쟁조정규정에 따라 WIPO 중재센터 혹은 조정부의 결정에 불복하여 관할권 있는 법원에 소를 제기할 수 있으며, 이는 조정절차의 피신청인에게 주어지는 권리이다. 그러나 소권은 신의칙에 반하여 행사되어서는 안 된다. 사이버스쿼터 즉 부정한 도메인이름 등록자는 도메인이름을 등록하여 사용할 정당한 권원이 상표권자에게 있다는 점도 잘 알고 있으며, 도메인이름 등록자에게는 분쟁대상 도메인이름들을 부정경쟁목적으로 사용할 의사가 있으며, 상표로서 사용할 의사 역시 있다고 하겠다. 사이버스쿼터가 분쟁대상 도메인이름들을 등록한 유일한 목적은 상표권자가 사용할 필요가 있는 이 사건 도메인이름을 선점함으로써 상표권자가 분쟁대상 도메인이름을 등록하지 못하도록 방해하고, 그럼으로써 도메인이름의 양도 대가로 상표권자에게 거액을 요구하여 부당이득을 취하고, 나아가 소비자들에게 부정한 도메인이름 등록자가 오래전에 도메인을 등록했기때문에 도메인이름 등록자만이 합법적인 권리자라는 잘못된 정보를 제공하여 소비자들을 오인혼동케 하려는 목적을 가지고 있을 수도 있다 하겠다. 도메인이름 등록자가 이렇게, 분쟁대상 도메인이름에 대한 부정경쟁목적 혹은 상표권 침해 이외의 어떠한 정당한 사용의사도 없으면서, 상표권자에게 고액의 금전을 받아 낼 목적으로 도메인이름 이전청구권 부존재 확인소송을 제기하는 것은 소권을 남용하는 것으로서 신의칙에 반하는 행동이므로 부적법 각하하는 것이 최선의 해결책이라고 할 수 있다. People who want to settle disputes related to register and usage of '.com' domain name, generic top level domains (hereinafter 'gTLDs') can apply to mediate said disputes to World Intellectual Property Organization Arbitration Center and other organizations certified by Internet Corporation for Assigned Names and Numbers. Besides, a person who likes to mediate disputes with respect to register and use of '.kr' domain name, country code top level domains (hereinafter 'ccTLDs') can submit an application to Internet address Dispute Resolution Committee (hereinafter "IDRC"), complying the rules of mediating disputes related to domain name. A domain name registrant is supposed to agree with a contract, including provisions to force him or her to agree mandatory mediation when he or she enters into a contract with a registrar. Trademark holders generally apply to mediate disputes to WIPO Arbitration Center or other certified organization for gTLDs and IDRC for ccTLDs. Usually, WIPO Arbitration Center decides that a registrant must transfer domain name to Trademark holder, and IDRC decide that a registrar must remove the register of registrant's domain name. However, as a right, a registrant can bring a lawsuit to appeal the decision of WIPO Arbitration Center or IDRC to the applicable court. However, any person cannot bring a lawsuit against the principle of good faith. An unclean registrant, so-called cyber squatter, knows that trademark holder has a right to register domain name and use it as a trademark. What is more, a registrant has intent to use the domain name with the anti-competitive purpose and to use domain name by infringing trademark. The purpose for cyber squatter to register domain name is to hinder a trademark registrant from registering disputed domain name by squatting said domain name, and then said cyber squatter to take excessive profits by blackmailing of trademark holder's money. Moreover, a cyber squatter using domain name as trademark may confuse customers, and give potential clients wrong recognition since he or she had already registered domain name before trademark registrant use domain name. Without any intent to use domain name as appropriate way except anti-competitive purpose or trademark infringement, a domain name registrant's bringing a lawsuit to blackmail of trademark holder's money is to misuse a right to sue, and it is against the principle of good faith. If a registrant, who has a intent to squat, wants to reserve and delay the administrative process to transfer domain name for gTLDs and to remove said registrant's register for ccTLDs, and he or she sues a trademark holder to decide whether trademark holder has a right to claim for prohibition of the use of domain name and whether trademark registrant has a right to claim for transfer of domain name, the court should dismiss not only a claim related to the right to claim for ban of the usage of domain name, but a claim with respect to the right to claim for transfer of domain name as a defense of trademark holder before the merit as well. Dismissal for the claim related to the right to claim for transfer of domain name as a pretrial defense of trademark holder is the best resolution to settle domain name disputes now.

콘크리트의 특성에 미치는 플라이애쉬 혼입방법에 관한 실험적 연구

이정섭,김성진,송하영,김을용 大田産業大學校 2001 한밭대학교 논문집 Vol.18 No.-

The purpose of this study is to inverstigate the influence of proportioning methods of fly ash on the properties of concrete. The laboratory test items were compressive strength and non-destructive test in hardened concrete, also slump, flow, air contents and mixing temperature in fresh concrete. The test results presented that the fly ash concrete in case of the substitute of 25% for fine aggregate contents had the best properties at the both W/C 40 and 60%.

Urapidil, Labetalol의 투여가 기관내 삽관시 심혈관계에 미치는 영향

신용섭,윤석화,손수창,이원형,이정은,황원재,김만수,김영주,김혜자,최세진 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

We have examined the comparative efficacy of small doses of intravenous urapidil and labetalol in blunting hemodynamic response to endotracheal intubation and surgical incision in 30 patients without cardiovascular diseases. After intravenous urapidil 0.2 mg/kg or labetalol 0.2 mg/kg anesthesia was induced with thiopental 5mg/kg. Endotracheal intubation was facilitated by vecuronium 0.15 mg/kg with priming principle and anesthesia was maintained with enflurane and nitrous oxide in oxygen. Systolic, diastolic and mean arterial pressure and heart rate were measured before administration of the drugs, 5 minute after administration, just prior to endotracheal intubation and 1, 3, 5, 10 minute after intubatin. Also the peak blood pressures and heart rate within 10 minutes after surgical incision were measured. Endotracheal intubation and surgical stimulation were associated with significant increases in blood pressures and heart rate in both urapidil and labetalol group. Comparison of the changes in systolic, diastolic, and mean artrial pressures and heart rate between urapidil and labetalol group showed no significant difference except peak systolic pressure after surgical incision. It is concluded that the pressor response to endotracheal intubation and surgical stimulation are not influenced significantly by urapidil 0.2 mg/kg or labetalol 0.2 mg/kg. However, urapidil and labetalol preloading may be similarly effective in the blunting of the increases in blood pressures with larger doses of the durgs during anesthetic induction.

최근 10년간 마취에 관한 임상적 고찰

신용섭,임정혁,최세진 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.1

To evaluate the specificity and the historical trends of the anesthetic agents and anesthetic methods used in Chungnam National University Hospital, anesthetic experiences of 21, 595 in total performed at Chungnam National University Hospital from January 1977 to December 1986 were analyzed stastically and clinically according to age, sex, surgical department, physical status, elective and emergency surgery, premedicants, induction agents, anesthetic methods, main anesthetic agent, anesthetic technique and muscle relaxants. The results were as follows: 1. The number of cases has been steadily increased. Considerable increase in the cases has been noted since 1985, when Chungnam National University Hospital was enlarged. 2. The distribution of the number of patients according to the age were 15, 332 cases (70. 9%) in the group of 16-60 years. 3. There were 12, 109 male (56. 1%) and 9,486 female (43.9/'0) cases. 4. 6, 128 cases (28.4%) were in general surgery, 4, 276 cases (19. 8%) in orthopedic surgery, 3, 138 cases (14.5%) in OB-GY and 1, 888 cases (8.7%) in ENT in order of numbers. 5. According to the ASA classification of physical status, most of the cases were belonged to the class 1 (33. 9%) class 2 (26.6%), class 1E (14.2%) and class 2E (12.5%). Almost cases were relatively in good physical status. 6. The rate of elective to emergency was 66. 5% (14, 370 cases) to 33. 5% (7, 225 cases). 7. As premedicants, a group of atropine, diazepam and hydroxyzine (33.4%) was given most frequently. Recently, glycopyrrolate has been used increasingly. 8. Thiopental sodium has been mainly used for intravenous induction agent. 9. General anesthesia has been used as the main method of anesthesia. 10. Halothane was the most commonly used anesthetic agent. Since 1979, the use of enflurane has been increased yearly. 11. The semiclosed circle breathing system was mainly employed. 12. Succinylcholine was given to most cases. Pancuronium was the most commonly used nondepolarizing muscle relaxant. Vecuronium has been used since 1986.

GIS를 이용한 아파트단지 통신장애의 시뮬레이션

김진택,엄정섭 경북대학교 사회과학대학 지리학과 2005 地理學論究 Vol.- No.24

기존의 전파 장애 예측 모델은 대부분이 외국의 학자들에 의해 개발되었고 2차원 특성을 지니고 있어 한국의 전형적인 지형에 적용하는 데 많은 한계를 가지고 있었다. 기존의 모델에서는 아파트 단지가 전파의 확산의 관점에서 영향을 미치는 독립적인 공간객체로 다루어지지 않았다. 재난 재해 등의 긴급상황에서 아파트 지역에서 교신 장애가 발생할 경우 문제의 원인을 규명하여 대처방안을 마련하는 데 상당한 한계가 있었다. 본 연구에서는 3차원 GIS와 가상현실의 기법에 의거하여 아파트의 배치형태 등 공간 특성을 반영한 한국의 전형적인 도시에서 발생하는 전파 장애에 대한 예측모델을 개발하였다. 본 연구결과는 아파트 건설 당시 전파 장애의 문제를 근본적으로 제거할 수 있는 배치형태를 결정하는 데 도움을 줄 수 있을 것이며 전파 기지국의 배치나 전파 장애지역의 해소 방안을 마련하는 데 중요한 참고자료가 될 수 있을 것으로 사료된다. Existing radio-wave prediction models do not accomodate Korean topographic elements as well as three-dimensional techniques, because they're developed by two-dimensional approach with various natural and artificial features based on foreign landform. At the model, apartment complex is treated as each general building and not classified by a propagation environment. Consequently, it has limitations for radio-wave operators to make correct decision with existing prediction models of radio-wave, which don't fit wireless network construction and application within the country. To solve these limitations, the apartment's radio-wave path loss model(GARP) was developed by simulating the typical Korean urban environment in GIS three-dimension virtual space. It can be used the apartments' construction, and collecting basic information about the Radio-wave environment to make an impact evaluation. By using the GARP model and mapping a value of wave path loss on real topographical space with GIS visibility, this study will ultimately contribute operators to deciding that design of a wireless service region, judgment about a shadow area of radio-wave, location selecting of the best suited base station or repeater station.

출아효모에서 TAR 클론닝법을 이용한 고등동물의 게놈으로부터 특정 염색체 부위의 분리

박정은,윤영호,이윤주,김광섭,윤세련,안태진,임선희,선우양일 동아대학교 기초과학연구소 2003 基礎科學硏究論文集 Vol.20 No.1

복잡한 게놈 분석에 용이하도록 효모의 인공 염색체(YAC) 클론닝 시스템은 발달되어왔다. YAC은 박테리아의 인공 염색체(BAC)보다 더 큰 단편을 클론닝할 수 있고, 또한 클론된 단편을 쉽게 변형시킬 수 있다. 형질전환과 연계된 재조합법(Transformation-Associated Recombination; TAR)은 게놈 라이브러리를 만들지 않고 직접 게놈 DNA로부터 분리하고자 하는 유전자나 특정 염색체 부분을 클론닝 할 수 있는 방법이다. 이 방법은 spheroplast transformation을 수행하는 동안, 목적으로 하는 유전자의 5' 그리고 3' 염기 배열(hooks)을 지닌 TAR 벡터와 게놈 DNA 사이에서 일어나는 상동성 재조합에 의해 이루어진다. 효모 내의 in vivo 재조합을 이용한 TAR 클론닝법은 복잡한 게놈으로부터 목적의 염색체 부분을 원형 YAC의 형태로서 선택적으로 분리할 수 있다. 그러므로 TAR 클론닝 법은 특정 염색체로부터 YACs을 만드는데 매우 유용하여, 전체 게놈으로부터 특이적 유전자나 유전자의 family를 분리하는데 효과적인 방법으로 사료된다. Yeast artificial chromosome (YAC) cloning systems have advanced the analysis of complex genomes considerably. They permit the cloning of larger fragments than do bacterial artificial chromosome systems, and the cloned material is more easily modified. Transformation-associated recombination (TAR) is a cloning technique that allows specific chromosomal regions or genes to be isolated directly from genomic DNA without prior construction of a genomic library. This technique involves homologous recombination during spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences (hooks). Using in vivo recombination in yeast, TAR cloning selectively isolates, as circular YACs, desired chromosome segments or entire genes from complex genomes. We propose that TAR cloning can provide an efficient means for generating YACs from specific chromosomes and that TAR cloning may be useful for isolating families of genes and specific genes from total genome DNA.

루프 미러로 구성된 기준팔을 가지는 파장 스위핑 OCT 구현

김훈섭,남궁명우,이정렬,엄진섭 江原大學校 産業技術硏究所 2007 産業技術硏究 Vol.27 No.B

As a reflector of reference arm in OCT systems, a coated fiber reflector has mostly used. Instead, this paper has realized the swaept source optical coherence tomography system using a loop mirror within a reference ann. Through the expehments for a tilted thin glass plate, we found that the loop mirror is better than the coated fiber typed mirror for obtaining the clear image.