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저분자량 폴리에틸렌이민을 이용한 유전자 송달 및 발현 유효성 연구
정길재,박귀례,신지영,최한곤,오유경 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.4
Branched and linear polyethylenimines (PEIs) have been studied as efficient and versatile agents for gene delivery in vitro and in vivo. PEIs exist in a linear or branched topology and are available in a wide range of molecular weight (Mw). Most studies have been done using PEIs with Mw higher than 10Kd. This study was aimed to test the transfection efficiency and the cell viability following gene delivery using PEI of Mw 2Kd, a relatively lower Mw cationic polymer. We used murine interleukin-2(mIL-2) plasmid DNA complexed with branched PEI 2Kd or 25Kd, and transfected them into a myoblast muscle cell line, C2C12. The cellular uptake of mIL-2 plasmid DNA was determined using quantitative polymerase chain reaction. RNA transcript levels were studied in the myoblast cells. Our results show that PEI 2Kd was as effective as PEI 25Kd in celluar gene delivery and transfection efficiency in C2C12 cells. Moreover, MTT assay indicated that PEI 2Kd/DNA complexes did not significantly reduce the cell viability regardless of N/P ratios. These results suggest that PEI of Mw 2Kd might play a role as effective and low toxic nonviral vector systems for muscular cell lines.
서정길,김종수,김용태,이경식,한성현 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-
In this paper, we developed a Windows XP version off-line programming system which can simulate a track vehicle model in 3D graphics space. The track vehicle was adopted as an objective model. The interface between users and the off-line program system in the Windows XP's graphic user interface environment was also studied. The developing language is Microsoft Visual C++. Graphic libraries, OpenGL, by Silicon Graphics, Inc. were utilized for 3D Graphics.
혼합형 자가면역성용혈성빈혈과 동반된 Evans 증후군 1례
선길홍,윤찬영,박상곤,박경희,우정주,한경택,김진화,김영훈,정춘해,박치영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2
Evans syndrome is defined as a simultaneous or sequential occurrence of autoimmune hemolytic anemia and autoimmune thrombocytopenia. Autoimmune hemolytic anemia (AIHA) is usually induced by IgG warm autoantibody or cold-active IgM antibodies reacting specifically with antigens associated with a patient's RBC. AIHA is a fairly uncommon disorder, with estimates of the incidence at 1 to 3 cases per 100,000 per year. Mixed-type AIHA is a relatively uncommon form of AIHA, with studies noting the incidence of 7-8% among cases of AIHA. We experienced a patient, 46-year-old woman who was diagnosed having a very rare clinical presentation of mixed warm and cold antibody mediated Evans syndrome. She was treated with corticosteroid therapy only and has been maintaining a complete response for 15 weeks. 저자들은 혼합형의 자가면역성 용혈성 빈혈과 자가면역성 혈소판 감소증이 동시에 발생한 혼합형 Evans 증후군으로 진단하고 스테로이드 요법 후 혈액학적으로 회복된 상태로 15주가 지난 현재 steroid 5 mg/일 까지 감량한 상태에서 추적 관찰 치료중인 46세 여자 환자를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.
황적준,한길로,김윤신,서정욱 大韓法醫學會 1998 대한법의학회지 Vol.22 No.1
The corrected transposition of the great arteries is a rare congenital heart anomaly consisting of the discordant connections of both the atria to ventricles and the ventricles to the great arteries. But its circulation is physiologically corrected and therefore it is compatible with life unless there are associated lesions. Our case was associated with VSD, COA, and PDA. He was slightly cyanotic and irritable. Despite of oxygen supply, his symptoms were not improved and he eventually died in 52 hours after delivery. Medical record revealed severe respiratory and metabolic acidosis.
교류전해에칭용 에틸렌글리콜의 알루미늄박판의 부식억제 효과
박성민, 양정진, 김홍일, 김한주, 김성한, 신진식, 박수길 충북대학교 건설기술연구소 2008 충북대학교 건설기술연구소 논문집 Vol.27 No.1
In this study, additions of aluminium foil eching characteristics were compared with sulfuric acid for capacitor. sulfuric acid and ethylene glycol is used as addition, characteristics of funtional group from addition were guessed by comparing of aluminium foil eching characteristics. When sulfuric acid was added in HCI etching solution, corrosion of aluminum metal was inhibited by the chemical adsorption of sulfate ion, when ethylen glycol was added in HCI etching solution, corrosion inhibited by the chemical adsorption of OH^ion.
상사성을 고려한 배수재 설치 연약점토 지반의 원심모델링
유남재,홍영길,정길수,조한기 강원대학교 산업기술연구소 2007 産業技術硏究 Vol.27 No.A
This paper is results of experimental research on the effect of application of similarity related to permeability of soil on the consolidation behavior as centrifuge modeling of consolidation is performed with the centrifuge model facility. In this research, the permeability of soil was controlled by changing the viscosity of porewater as the mixed water with glycerin was used during the centrifuge model experiments. The effect of drainage path on consolidation was investigated by installing the vertical drains. A serise of centrifuge model tests with conditions of single vertical and radial horizontal drainage were carried out. Kaolinite and Jumunjin standard sand were used as soft clay and surcharges respectively during tests. For testing condition of single vertical drainage considering similarity of permeability, it was found that consolidation with mixed porewater with glycerin was delayed in comparisons with test results with water only. For conditions of horizontal drainage with vertical drains, a low permeability by changing the viscosity of pore water resulted in delayed degree of consolidation at an initial stage of consolidation. But, it predicted not much differences in settlement as long as the consolidation time was sufficiently long enough to finish consolidation. Consequently, it was found that similarity in permeability should be considered to be critical for the case of centrifuge model experiments related to consolidation with long drainage path.
황적준,김정아,한길로 大韓法醫學會 1999 대한법의학회지 Vol.23 No.1
The attitude toward the use of autopsies affects the incidence of performance of th procedure. In foreign countries, many studies on this subjects have been conducted. However, in Korea, no study has been reported. We hypothesized that the general university students may be negative, whereas the medical college students may have positive attitude toward the use of autopsies. In contrary to our hypothesis, there existed only a little difference for the attitude toward conducting an autopsy between the general university students and the medical students. Both groups had positive attitudes toward autopsy. When the medical students become medical doctors, the main reason for not requesting patients to be conducted an autopsy is that their family members may feel offended. These results show that the low incidence of autopsy cases in Korea is caused not by the attitude of the general population, but by the attitude of medical doctors. Thus, we should make every effort to increase the incidence of autopsy by educational programs and discussions about autopsy and death.
개 이자의 Somatostatin 양성세포의 발달에 관한 면역조직화학적 연구
장인엽,윤상필,정윤영,김종중,문정석,한길현,김영택,안계훈,선희매 조선대학교 부설 의학연구소 1998 The Medical Journal of Chosun University Vol.23 No.2
Cells immnunoreactive for somatostatin(SOM) and glucagon(GLU) were investigated in postnatal day 0, 7, 14, 28, 90, 180 and adult canine pancreas using light microscopic immunohistochemistry. Pancreatic SOM-immunoreactive cells were found in pancreatic acini, pancreatic duct, and pancreatic islet gradually. Pancreatic SOM-immunoreactive cells were more located in pancreatic parenchyme than pancreatic islet throughout pancreas by postnatal day 28. But there was change by the postnatal 3 months : pancreatic SOM-immunoreactive cells were more located in pancreatic islet than acini and duct. It is suggested that the number of the SOM-immunoreactive cells may not increase with age apparently. A small number of SOM-immunoreactive cells that showed GLU-immunoreactivity were also observed in canine pancreatic islet. The physiologic consequences of these changes remain to be determined. These results sugegest that SOM in the developing pancreas may relate to the secretion of pancreatic hormones.