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Background : Rehmannia glutinosa is a perennial herb belonging to family Scrophulariaceae. This study was breeding of R. glutinosa cultivar with insect tolerance and high-yielding. Methods and Results : ‘Chunggang’ is developed by the medicinal crop breeding team of National Institute of Horticulture and Herbal Science (NIHHS), Rural Development Administration (RDA), during the period from 2005 to 2017. The reproduction of Rehmannia glutinosa has been accomplished mainly by vegetative propagation with its seedlings. This cultivar was selected from seedling of RG10. The plant type of Chungang is some rising from ground. Regional yield trials conducted at three site from 2016 to 2017. The root yield of ‘Chunggang’ was 22.3 ton per hectare, which was increased 11% compared with ‘Jihwang 1’ (check variety). Also, ‘Chunggang’ has higher insect tolerance compared with ‘Jihwang 1’. Conclusion : ‘Chunggang’ is a insect tolerance and high-yielding Rehmannia glutinosa cultivar.
A novel lectin specific to N-acetyl-D-galactosamine as well as N-acetyl-D-glucosamine was isolated from Bryopsis plumosa and named as BPL-4. Sodium dodecyl sulfate polyacrylamide gel electrophorese (SDS-PAGE) and matrix-assisted laser desorption / ionization-time of flight (MALDI-TOF) mass spectrometry data showed that this lectin was a monomeric protein with molecular weight 12.9 kDa. The N-terminal amino acid sequences of the lectin were determined by Edman degradation and the full cDNA sequence encoding this lectin was obtained using the degenerate primers designed from the amino acid sequence. The size of the cDNA was 414 bp containing single open reading frame (ORF) encoding the lectin precursor. The homology analysis showed that this lectin might belong to H lectin group. BPL-4 showed high sequence similarity (60.6%) to BPL-3, which is a previously reported lectin from the same species. The comparative analysis on the lectin’s primary structure showed two conserved domains including one possible active domain of H lectin group.
Conventional methods for the isolation and purification of mRNA from Zygnema were unsuccessful because of its high amount of pigments and RNA interactive molecules. In particular, pigments were difficult to remove using conventional protocols because they interacted with RNA during pulverization of the materials. This resulted in total degeneration of RNA in two to three hours. To alleviate this problem, we developed an isolation method that utilized DEAE-cellulose resin. The pigments bound to DEAE anion exchange resin and separated from the RNA. Purified total RNA showed an yield of 50 μg per 100 mg of tissue with this method. The amplified 2nd strand cDNA was distributed 300 bp and over.
On 3 May 2020, an ML 3.1 earthquake occurred in Haenam, southwestern Korea, in an area devoid of recorded seismicity since instrumental observations began in 1978. Careful examination of the temporal occurrence of seismicity, and the magnitude distribution of the sequence before and after the ML 3.1 earthquake, indicates typical swarm-like behavior. The earthquake swarm started with an ML 0.6 event on 26 April 2020, intensified up to 3 May 2020, and abruptly terminated with an ML 1.0 event on 9 May 2020. The Pusan National University Geophysics Laboratory (PNUGL) deployed a temporary seismic array with eight threecomponent short-period instruments to monitor the short-lived bursts of seismicity. During the monitoring campaign, we detected > 700 microearthquakes by applying a matched-filter technique to the combined dataset produced by PNUGL, the Korea Meteorological Administration, and the Korea Institute of Ocean Science and Technology. We determined earthquake parameters for 299 earthquakes that were detected at four or more seismic stations. We also determined the focal mechanism solutions of the 10 largest earthquakes in the swarm using first-motion polarities with S/P ratios. The focal mechanism, hypocentral depth, and stress orientation of the largest earthquake in the sequence were also determined using waveform inversions. The distribution of earthquake hypocenters, together with focal mechanism solutions, indicates that the earthquake swarm activated deeply-buried faults (~20 km) oriented either NNE-SSW or WNW-ESE. We also report details of the temporary seismic monitoring network, including the instrumentation, detection of microearthquakes, and variations in event-detection threshold influenced by anthropogenic and natural noise fluctuations. We also discuss the limitations associated with lowering the detection threshold of microearthquakes by increasing the number of seismic stations or by adopting advanced event-detection techniques.
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The flora of resource plants in Middle and Northern region of Yangsan-si were investigated for 7 times from March to Sept., 2009. The study indicated that, based on voucher specimens, the flora of this area consist of 427 taxa in total; 90 families, 256 genera, 376 species, 4 subspecies, 41 varieties and 6 forms. The resource plants in this area were categorized by their use into 9 groups inclusive of 1 unidentified group. Resources plants which were investigated in this area were 167 edible, 132 pasturing, 118 medicinal, 98 stainable, 52 ornamental, 15 timber, 6 fiber, 2 industrial taxa and 101 unknown resource plants, respectively. Also, there were remarkable plants such as 16 taxa of Korean endemic plants and 36 taxa of specific plants which were designated by the Ministry of Environment. Furthermore, 22 taxa of naturalized plants were observed in this investigated area where Urban Indexn UI) was 8.9%. Although the ecological status of investigated area was comparatively well conserved, the degree of (UI) was relatively high. Based on the results of this investigation, UI has been rapidly increased due to urbanization and construction of recreation objects in this area. 2009년 3월부터 2009년 9월까지 경상남도 양산시 일대의 관속식물 및 자원식물분포조사를 수행하였다. 본 조사를 통해 확인된 조사지역 내의 소산 관속식물은 90과 256속 376종 4아종 41변종 6품종으로 총 427분류군으로 확인되었다. 조사 지역에서 분포하고 있는 427분류군의 식물자원Table 4. The list of 5th to 3rd degree taxa of specially designated plants by Ministry of Environment Degree Family Scientific name Korean name ⅣRosaceae Prunus davidiana (Carrière) Franch. 산복사Leguminosae Wisteria floribunda (Willd.) DC. 등Araceae Symplocarpus renifolius Schott ex Miq. 앉은부채III Saxifragaceae Philadelphus pekinensis Rupr. 애기고광나무Leguminosae Indigofera pseudotinctoria Matsum. 낭아초Leguminosae Vicia chosenensis Ohwi 노랑갈퀴Leguminosae Lespedeza juncea (L.f.) Pers. 땅비수리Orobanchaceae Phacellanthus tubiflorus Siebold & Zucc. 가지더부살이Cyperaceae Carex gifuensis Franch. 애기감둥사초II Saxifragaceae Saxifraga oblongifolia Nakai 참바위취Rosaceae Aruncus dioicus var. kamtschaticus (Maxim.) H.Hara 눈개승마Rosaceae Potentilla dickinsii Franch. & Sav. 돌양지꽃Violaceae Viola orientalis (Maxim.) W.Becker 노랑제비꽃Violaceae Viola tokubuchiana var. takedana (Makino) F.Maek. 민둥뫼제비꽃Labiatae Clinopodium micranthum (Regel) Hara 두메층층이Caprifoliaceae Weigela florida (Bunge) A.DC. 붉은병꽃나무Compositae Cirsium schantarense Trautv. & Mey. 도깨비엉겅퀴IAspleniaceae Asplenium ruprechtii Kurata 거미고사리Pinaceae Pinus koraiensis Siebold & Zucc. 잣나무Pinaceae Abies holophylla Maxim. 전나무Betulaceae Alnus sibirica Fisch. ex Turcz. 물오리나무Fagaceae Quercus variabilis Blume 굴참나무Ranunculaceae Clematis patens C.Morren & Decne. 큰꽃으아리Chloranthaceae Chloranthus japonicus Siebold 홀아비꽃대Aristolochiaceae Asarum maculatum Nakai 개족도리풀Saxifragaceae Chrysosplenium flagelliferum F.Schmidt 애기괭이눈Rutaceae Dictamnus dasycarpus Turcz. 백선Aquifoliaceae Ilex macropoda Miq. 대팻집나무Celastraceae Euonymus macropterus Rupr. 나래회나무Primulaceae Primula sieboldii E.Morren 앵초Caprifoliaceae Weigela praecox (Lemoine) L.H.Bailey 소영도리나무Liliaceae Erythronium japonicum (Baker) Decne. 얼레지Liliaceae Polygonatum inflatum Kom. 퉁둥굴레Liliaceae Hosta minor (Baker) Nakai 좀비비추Orchidaceae Cephalanthera falcata (Thunb.) Blume 금난초IV: 3 taxa, III: 6 taxa, II: 8 taxa, I: 18taxa 유용도를 분석한 결과 식용자원식물이 167종류, 섬유용 자원식물이 6종류, 약용자원식물이 118종류, 관상용 자원식물이Table 5. Naturalized Plants in Yangsan-si No. Family Scientific name Korean name 1Amaranthaceae Amaranthus blitum L. 개비름2Amaranthus patulus Bertol. 가는털비름3Phytolaccaceae Phytolacca americana L. 미국자리공4Fallopia dumetorum (L.) Holub 닭의덩굴5Rumex acetosella L. 애기수영6Rumex crispus L. 소리쟁이7Leguminosae Astragalus sinicus L. 자운영8Trifolium pratense L. 붉은토끼풀9Trifolium repens L. 토끼풀10Euphorbiaceae Euphorbia maculata L. 큰땅빈대11Euphorbia supina Raf. 애기땅빈대12Onagraceae Oenothera biennis L. 달맞이꽃13Cruciferae Lepidium apetalum Willd. 다닥냉이14Lepidium virginicum L. 콩다닥냉이15Thlaspi arvense L. 말냉이16Convolvulaceae Ipomoea purpurea Roth 둥근잎나팔꽃17Scrophulariaceae Veronica arvensis L. 선개불알풀18Veronica persica Poir. 큰개불알풀19Compositae Ambrosia artemisiifolia L. 돼지풀20Aster pilosus Willd. 미국쑥부쟁이21Bidens frondosa L. 미국가막사리22Conyza canadensis (L.) Cronquist 망초23Conyza sumatrensis E.Walker 큰망초24Erechtites hieracifolia Raf. 붉은서나물25Erigeron annuus (L.) Pers. 개망초26Erigeron strigosus Muhl. 주걱개망초27Galinsoga ciliata (Raf.) S.F.Blake 털별꽃아재비28Galinsoga parviflora Cav. 별꽃아재비29...