뇨(尿)중 3-Methylhistidine 함량의 측정과 이용

정수현,서형주,김윤숙,이효구,강덕호 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.2

기존의 방법을 일부 수정하여 뇨중 3-methylhistidine을 분석하였다. 뇨중 3-methylhistidine을 fluorescamine 유도체화하여 HPLC에 주입하고 C_18 column과 10 mM acetonitrile/sodium phosphate buffer(pH 7.5)로 분리·용출시켜 형광검출기로 측정하였다. 3-methylhistidine의 체류시간은 7분 이내이었으며, histidine과의 분리상태도 서로 간섭함이 없이 양호하였다. 뇨에 3-methylhistidine을 첨가하고 이를 분석하였을 때의 회수율은 93∼106%로 높은 수준이었다. 체육학과 남학생중 웨이트 트레이닝 단련자와 비단련자를 대상으로 조사한 단기간의 웨이트 트레이닝에 따른 뇨중 3-methylhistidine 함량의 변화는 두 집단 모두 웨이트 트레이닝후의 3-methylhistidine 분비량이 유의하게 증가하였다. A modified method is given for the precolumn derivatization and subsequent high-pressure liquid chromatographic seperation of 3-methylhistidine from urine. The elution contained isocratic solution with acetonirile and 10 mM sodium phosphate(pH 7.5) requires less than 7 min. The recoveries of 3-methylhistidine from urine control were 93% to 106%. 3-Methylhistidine determinations were performed on urine samples from volunteers who were both male trained and non-trained physical undergraduates. As the result, urinary 3-methylhistidine content of volunteers increased significantly after weight training.

HBsAg 양성혈청에서의 HBeAg 및 anti-HBe 양성율과 ALT와의 관계 고찰

문희주,윤기은,박정오,배형준,최범열 서울보건대학 1996 서울보건대학 부설 한국보건과학연구소 논문집 Vol.3 No.1

The authors investigated HBeAg and anti-HBe in 1,000 cases(male 941, female 59) of HBsAg positive among the 225,512 blood donors in Seoul Nambu Blood Center in 1994. HBeAg and anti-HBe was detected by the method Enzyme Immunoassy. The results obtained were as follows ; 1. HBeAg and anti-HBe positive rates were detected 498 cases(49.8%) and 445 cases(44.5%) respectively among 1,000 cases HBsAg positive blood donors. 2. In HBsAg positive carriers, HBeAg positive rates were not significantly different between anti-HBe positive rates, but in sex were significantly different between HBeAg positive rates and anti-HBe positive rates. 3. HBeAg positive 498 cases were analyzed with age and it was found percentages of positive rates were 63.6% in 1st decade, 53.7% in 2nd decade, 20.6% in 3rd decade, 19.6% in 4th decade and 8.3% in over 5th decade, but anti-HBe positive 447 cases were 32.4% in 1st decade, 40.5% in 2nd decade, 69.2% in 3rd decade, 78.3% in 4th decade and 91. 7% in above 5th decade. 4. HBeAg positive rate decreased stepwise with age, while anti-HBe positive rate increased stepwise with age. 5. ALT abnormal cases in the HBeAg positive donors were higher than normal cases, but ALT normal cases in the anit-HBe positive donors were higher than abnormal cases.

병원내 생리기능 검사실의 적정 업무환경에 관한 연구

배형준,윤기은,김승곤,나동진,문희주,김태전,박정오 서울보건대학 1999 서울보건대학 부설 병원경영연구소 논문집 Vol.4 No.2

The purpose of this study was to suggest guideline for optimal work environment of physiological laboratory in hospital. Therefore this study was carried out to analyze the actual condition of laboratory system, major equipments and persons. The period of survey was from September to November 1997 and the objects were 123 laboratory. The major results of this study were as follows. 1. The optimal areas of general ECG room, exercise ECG room and echocardiology room were all 9-18㎡, that of Holter ECG room was 3-12㎡. The optimal areas of neurosystem laboratory were all 9-12㎡, those of respiratory system laboratory were all 9-18㎡. 2. The optimal number of persons laboratory of hospital 500 beds and less were 2∼3, those of 501∼700 beds were 3∼4.701∼1.000 beds were 4∼5 and over 1.001 beds were 7∼8 persons in circulatory system laboratory. The optimal number of persons laboratory of hospital 500 beds less than were 1∼2, those of 501∼700 beds were 3∼4, 701∼1.000 beds were 6∼7 and over 1.001 beds were over 8 persons in neurosystem laboratory. The optimal number of persons laboratory of hospital 500 beds and less 1. those 501∼1.000 beds were 1∼2 and over 1.001 beds were 2∼3 persons in repiratory system laboratory. 3. The optimal number of equipments of general ECG were 2∼3 in the 500∼1.000 beds hospital. there were 3∼4 units in the 1,001 beds. The optimal number of equipment EEG were both 1∼2 in the hospital 500 beds and less and 501∼700 beds. those were 4∼5 units in hospital over 701 beds. The optimal number of equipments of spirograph were both 1∼2 in the hospital 500 and less and 501∼1.000 beds. those were 2∼3 units in the over 1.001 beds. 4. In view of speciality and legal problem of physiofunctional test. we sugget that non-licensee were so quickly change place licensee in the interests of optimal work environment. 5. We suggest that to upgrade qualitative level of domestic equipment and to the more amplify utilization with respect to domestic equipment for the purpose of reducing loss of foreign money.

자가 골수 기질세포이식을 이용한 대퇴골두의 무혈성 괴사 및 장관골 골낭종의 치료

이승구,권순용,정진화,정도현,이주형,최숭욱 대한정형외과학회 골.연부조직이식학회 2003 대한골.연부 조직이식학회지 Vol.3 No.2

Natural Toxin의 안전성 평가연구 : Ochratoxin A의 면역독성 Immunotoxicity of ochratoxin A

김주일,한형미,정혜주,김형수,김환묵,정승태,박재현,강선경,김진호 식품의약품안전청 1997 식품의약품안전청 연보 Vol.1 No.-

Ocllratoxin A는 fenicl'flrium과 rtsr☞rgiffus 속 등의 곰팡of가 생성하는 mycotoxin으로서 세포매기성 및 체액성 면역기능에 슨상을 일으키는 것으로 알려져 있다. 본 연구는 OTA가 면역계에 미치는 영향과 OTA의 면역 독성 억제 효과가 있는 물질을 검색하기 위하며 6~7 주령의 Balb/c mice 비장세핀에 T 및 B 세포의 mitogen인 Con A와 LPS로 처리하고 3일간 배양한 후 비장세포의 분화정도를 MTS를 처리하여 관찰하였으며, OTA를 투여한 마우스의 면역장기 형리조직학적 견화를 관찰하였다. 마우스의 비장세포 (2xtD6cel1/rnf)에 OTA(1~6μg/ml)를 상에서 혼합하여 Con A와 LPS로 자극시킨 결과 임파구는 Sfg/mf이상에서 거의 모든 세포가 분화되지 않았다. mice에 OTA (1,5 및 10mg/11g)를 2일 간격으로 6회 복갚 주사한 후 비장세포를 분리하여 Con A와 LPS로 처리한 결과 Smg 투여군에서는 6회 투여 혼에 분화가 억제되었고, 10mg 투여군에서는 3회 및 6회 투여군에서 분화가 억제되었단. Ochratoxin A의 면역독성 억제 효과가 있는 물질을 검색하기 위하여 in vitro 실험에서 마우스의 비장세포 (2×10" cell/mf)에 OTA (3fg/mf)와 L-phenylalanine, indole-3-carbinol, L-ascorbic acid,piroxicaTn, curcumin 및 arethylsalicylic acid를 혼합하고 동일 처리한 결과 L-ascorbic acid, L-phenylala-nine 및 Indole-3-carbinol은 OTA의 면역독성 발현을 운의성 있게 억제하였다. Balt/c mice에 OTA (10mg/kg)와 l'n pifro실험에서 억제효파가 확인된 L-ascorbic acfd, L-phenylalanine 및 indole-3-carbinol을 2일 간격으로 동시에 3회 복강주사하여 관찰한 결과 indote-3-carbine께서 엮제효과를 관찰할수 있었다. Ochratoxin A를 투여할 마우스의 비장 및 홍선에서는 임파구의 빈도가 줄었으며 특히 비장제서는 starry-sky 세포가 증가됨을 관찰할 수 있었다. 이러한 결자로 OTA는 비장세포의 T 및 B 임파구의 분열능을 억제시 키며, 면역장기의 임파구에 손상 야기시키므로서 면역기능을 저하시키고 OTA에 의한 면역독성은 indole-3-carbinol이 경감시킴을 관찰하엿다. Ochratoxin A (OTA) is a Tnyeotoxin produced by several fungal species iBcluded the genera fs4pergiffug and f☞HiciHiHuL and known as one of the major environmental contaminants. Tn thepresent study, the effects of OTA on the imraune system and the prevention of the OTA-inducedimmunosuppression by protective agents were studied in Balb/c rnicf:. Splenocytes were isolated and theproliferative responses to concanavalin A (Con A) and lipopolysaccharide (LPS) were measured in thepreseEce of 1 to 6fg/mf OTA. Sfg/mf OTA completely btocked both Con A and LPS-stimulatedmitogenic responses, causing approximately 50% inhibition at 3fe/mf OTA. This suppression of mitogen-induced proliferative resplonses observed id uifro was reproduced in mice treated with ☞rA in rioafntraperitoneal administration of 5, IDmg/kg OTA 3 to etimes every other day significantly decreasedboth Con A- aud LPS-inttuced mitegenic responses. The effects of possible protective agents on theOTA-induced suppression c)』 mitogenic responses were examined both in uifro and in viua Amoag 6 pos-sible protective agents (1,-phenylalanine, L-ascorbic acid, indole-3- carbinol, curcumin, firoxicam,acethylsaricylic acid) employed in the present study, L-phenylalanine (100ff), L-ascorbic acid (100#M) and iBdote-3-carbinel t 10Df) showed significantly protective effects on the OTA-iBduced suppres-sion of mitogenic responses id uifro. Howeuer, when the protective of·fects of these agents were examinedin rr'ua only indole-3-carbinol showed significantly protective respoases. It has been ebserved that Iyin-phatic cell population in thc spleen and thymus was decreased in raice given OTA by hematoBylin-eosinstaining. These data indicate that OTA suppresses the cell-mediated and humoral immune functioBsand this OTA-iBduced supflression of immune functions can he alleviated by indole-3-carbinol.

Nano-Calcium Ameliorates Ovariectomy-Induced Bone Loss in Female Rats

Hyeon-Son Choi,JeungHi Han,Seungsik Chung1,Yang Hee Hong,and Hyung Joo Suh 한국축산식품학회 2013 한국축산식품학회지 Vol.33 No.4

In this study, we examined the effects of organic types of calcium derived from oyster shell (OS-Ca) and nano-calcium (Nano-Ca) on the bio-availability and physiological responses associated with bone health in ovariectomised rats. Increased body weight, which is one of the physiological effects of ovary removal, was significantly recovered by Nano-Ca treatment (p<0.05). The reduced calcium level in the liver in ovariectomised rat was increased significantly with OS-Ca and Nano-Ca treatment (p<0.05), suggesting improved calcium bio-availability. Alkaline phosphatase (ALP), osteocalcin, and deoxypyridinoline (DPD) were analysed as biochemical markers of bone metabolism and health in the presence or absence of OSCa and Nano-Ca. ALP, osteocalcin, and DPD levels increased following ovary removal and tended to decrease after treatment with Nano-Ca, indicating that Nano-Ca induces favourable bone metabolism. This result was reflected in the recovery of bone mineral density (BMD) and bone mineral content (BMC) of the femur after Nano-Ca treatment following ovary removal. Taken together, our data show that the tested calcium treatments, especially using Nano-Ca, enhanced the bioavailability or absorption of calcium and positively affected bone metabolism in ovariectomised rats.

Fluoxetine이 Schedule-Induced Polydipsia가 유발된 백서 뇌에서 Tyrosine Hydroxylase 발현에 미치는 영향

이기철,이정호,최영민,정주호,정홍경,이용민,김도형,이대환 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.2

연구목적: Fluoxetine은 serotonin을 매개하여 간접적으로 dopamine 신경전달기능을 억제한다고 추정되고 있다. 또한 운동장애에서 운동기능의 악화를 유발한다고 알려져 있다. 그러나 신경세포체에서 fluoxetine이 dopamine에 어떠한 영향을 주는지는 아직까지 확실치 않다. 저자들은 schedule-induced polydipsia를 유발시킨 백서 뇌의 흑질, 복부피개영역, 미상핵에서 tyrosine hydroxylase(TH) 발현이 저하됨을 발견하였다. 이를 통해서 fluoxetine이 백서 뇌의 dopamine 기능에 긍정적인지 혹은 부정적인지를 규명하고자 하였다. 방법: 4주간의 schedule-induced polydipsia 과정을 거친 백서에서 면역죄치화학적인 방법으로 흑질, 복부피개영역, 미상핵의 tyrosine hydroxylase 발현이 저하됨을 확인한 후, 실험동물들에게 fluoxetine 10mg/kg를 3주간 복강내 주사하였다. 실험백서들을 희생시켜 뇌 조직을 적출하여, TH 면역조직화학 염색법을 이용하여 흑질, 복부피개영역, 그리고 미상핵의 TH 면역반응세포를 관찰하고 이를 정상백서와 비교하였다. 결과: 1) 다갈증이 유발된 백서의 흑질, 복부피개영역, 미상핵에서 tyrosine hydroxylase 발현이 정상백서 보다 저하됨을 관찰하였다. 2) 3주간에 걸친 fluoxetine 투여후 흑질, 복부피개영역, 미상핵의 tyrosin hydroxylase 발현이 다시 증가하는 소견을 보였다. 결론: Fluoxetine 만성투여가 흑질, 복부피개영역 그리고 미상핵의 tyrosin hydroxylase를 증가시키는 소견을 얻었다. 이러한 결과는 임상에서 dopamine 결핍과 연관된 질환들에서 fluoxetine을 만성투여하면 운동기능을 포함한 증상들의 개선을 가져올 수도 있다고 추정된다. Objective: It has been suggested that fluoxetine inhibits the dopaminergic neurotransmission by serotonergic mediation. And also, it has been shown to inhibit synthesis of DOPA in dopamine-rich areas of the rat forebrain. These dopamine-antagonistic capacity of fluoxetine is only supported by anecdotal report that the increased amount of motor disability in patients with idiopathic Parkinson's disease after exposure to fluoxetine. However, there is still no evidence of the direct effect of fluoxetine on dopaminergic neuronal cell body in the substantia nigra, VTA, caudate & putamen. This study was designed to evaluate the effects of fluoxetine in rat brain which showed decreased numbers of dopaminergic neuronal cell body induced by schedule-induced polydipsia(SIP). Method: We incidentally found that 4 weeks of schedule-induced polydipsic rats revealed the suppression of tyrosine hydroxylase expression in the substantia nigra, VTA, caudate & putamen with the immunohistochemistric measures. After 3 weeks of intraperitoneal injection of 10mg/kg of fluoxetine to the schedule induced polydipsic rats, the tyrosine hydroxylase expression was also measured with immunohistochemistry. We compared the tyrosine hydroxylase expression among the normal control, the polydipsic rats, and the rats with fluoxetine treatment. Results: 1) By contrast with the control, the polydipsic rats revealed the evidence of decreased tyrosine hydroxylase expression in the substantia nigra, VTA, caudate & putamen. 2)After daily injection of fluoxetine for 3 weeks, the polydipsic rats showed increment of tyrosine hydroxyase expression in those areas. Conclusions: In previous studies, a great deal of results suggest that fluoxetine negatively influence the dopaminergic systems indirectly via serotonergic activation such as inhibition of dopamine synthesis or transport system. Although our results are obtained from rodents, we suggest that fluoxetine directly and positively enhance the dopamine system in the substantia nigra, VTA, caudate & putamen. The chronic adminstration of fluoxetine may be helpful to dopamine-depleted condition in clinical situations. We anticipate the replication studies of our findings and well-controlled clinical trial.

Natural Products,Organic Chemistry : Plant Growth-promoting Activity of Acremonium strictum MJN1 Isolated from Roots of Panax ginseng

Hyung Bum Lim,Yang Jo Chung,Ju Yun Bae,Dong Jin Kim,Hyung Jin Kwon,In Hyung Lee,Byung Chul Chung,Woong Sang Lee,Joo Won Suh 한국응용생명화학회 2000 Journal of Applied Biological Chemistry (J. Appl. Vol.43 No.2

Experimental Test of Gamma Emission Tomography to Interrogate Partial Defects within PWR Spent Nuclear Fuel

Hyung-Joo Choi,Yoon Soo Chung,Hyun Cheol Lee,Yong Hyun Chung,Chul Hee Min 대한방사선방어학회 2023 대한방사선방어학회 학술발표회 논문요약집 Vol.2023 No.4

Angiopoietin-1 promotes endothelial differentiation from embryonic stem cells and induced pluripotent stem cells

Joo, Hyung Joon,Kim, Honsoul,Park, Sang-Wook,Cho, Hyun-Jai,Kim, Hyo-Soo,Lim, Do-Sun,Chung, Hyung-Min,Kim, Injune,Han, Yong-Mahn,Koh, Gou Young American Society of Hematology 2011 Blood Vol.118 No.8

<B>Abstract</B><P>Angiopoietin-1 (Ang1) plays a crucial role in vascular and hematopoietic development, mainly through its cognate receptor Tie2. However, little is known about the precise role of Ang1 in embryonic stem cell (ESC) differentiation. In the present study, we used COMP-Ang1 (a soluble and potent variant of Ang1) to explore the effect of Ang1 on endothelial and hematopoietic differentiation of mouse ESCs in an OP9 coculture system and found that Ang1 promoted endothelial cell (EC) differentiation from Flk-1+ mesodermal precursors. This effect mainly occurred through Tie2 signaling and was altered in the presence of soluble Tie2-Fc. We accounted for this Ang1-induced expansion of ECs as enhanced proliferation and survival. Ang1 also had an effect on CD41+ cells, transient precursors that can differentiate into both endothelial and hematopoietic lineages. Intriguingly, Ang1 induced the preferential differentiation of CD41+ cells toward ECs instead of hematopoietic cells. This EC expansion promoted by Ang1 was also recapitulated in induced pluripotent stem cells (iPSCs) and human ESCs. We successfully achieved in vivo neovascularization in mice by transplantation of ECs obtained from Ang1-stimulated ESCs. We conclude that Ang1/Tie2 signaling has a pivotal role in ESC-EC differentiation and that this effect can be exploited to expand EC populations.</P>