Critical role of bile acid (BA) in the cellular entry and permissiveness of Hepatitis B virus in vitro

Hong‑Jai Lee,Bo‑Young Shin,Jae‑Seung Moon,Ailyn Fadriquela,Selikem Abla Nuwormegbe,Chun‑Chang Ho,Jin‑Su Shin,Jee‑Sang Yoon,Sang‑Kyou Lee,Soo‑Ki Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Lack of universal replication system for hepatitis B virus with narrow host range and organ tropism has hampered to uncover the pathogenesis of HBV. Previously, we reported the essentiality of humoral milieu and its components toward HBV and hepatitis C virus survival/viability in vitro. Of these components, the precise role of enterohepatic humoral milieu such as bile acid (BA) on HBV cultivation in vitro and in vivo is unknown. Objective We explored whether BA, specifically taurochenodeoxycholic acid (tCDCA) would directly regulate the viral DNA and surface antigen expression of HBV in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. Result We found that higher concentration of taurochenodeoxycholic acid (tCDCA) is able to preserve the genomic stability of HBV in cell-free DMEM, showing higher the surface antigenicity than taurocholic acid (tCA). In line, we found that in vitro cell culture condition (100 μmol/L of tCDCA coupled with 1 × 108 g e/mL HBV) would be optimal for HBV entry into target cells. Using this, human (HepG2, Huh7), and rodent (Hepa1c1c7, H4-II-E) hepatoma cell lines were infected by HBV, as evidenced by the presence of HBV biomarkers (HBsAg, and HBV DNA in culture supernatant, as well as HBcAg in cell). Further, cellular entry test revealed that HBV is able to infect 12 different non-hepatic cell lines regardless of species, and organ/tissue, consequently reproducing progeny as confirmed by HBV biomarkers. Last, reinfection test showed that the progenies of HBV from immortalized HepG2, and Hepa1c1c7 cells are able to enter into each or vice versa naïve HepG2, and Hepa1c1c7 cells with or without BA. Conclusion This study demonstrates that enterohepatic humoral milieu such as BA, specifically tCDCA would directly regulate HBV DNA and its surface antigen expression in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. This is the first note to render HBV permissive to human or rodent hepatic and non-hepatic cells via sole manipulation of humoral milieu, thus establishing the platform for in vitro robust replication system of HBV.

연령증가에 따른 혈압요소들의 좌심실비대 및 관동맥질환과의 관련성

박재석,박창규,박미영,박재형,김용현,나진오,신성희,서순용,홍순준,박성미,임홍의,김응주,서홍석,오동주,노영무 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

장거리 해저 광전송망 분산보상 설계 및 구현

유재덕(Jae Duck Yoo),박홍태(Hong Tae Park),신현식(Hyun Sik Shin),김천석(Chun Suk Kim),신윤호(Yun Ho Shin) 한국전자통신학회 2006 한국전자통신학회 논문지 Vol.1 No.1

현재 국내.외 기간 통신망은 급격히 증가하는 고속 대용량 트래픽을 효율적으로 멀리 전송하기 위해 광증폭기를 이용한 파장분할다중화방식 (DWDM:Dense Wavelength Division Multiplexing) 광전송 시스템 통신망을 구축하고 있다. DWDM광전송 시스템에서 고속, 대용량 및 장거리 전송을 하기 위해서는 기존 3R 광 중계기시스템에서 크게 중요시 않았던 광 fiber의 비선형(non-linearity) 전송제약 요소를 고려하여 통신망을 설계하여야 한다. 광통신망에서 장거리 전송 시 신호펄스 파형의 왜곡을 최소화하기 위해 영분산(zero-dispersion)을 구현하여야 하지만, 영분산으로 인하여 파이버의 비선형 현상이 나타나 신호 스펙트럼 왜곡 현상이 발생하는 문제가 있다. 즉 영 분산과 파이버의 비선형 사이에 서로 상충관계를 해결하여야 고속 대용량 트랙픽을 장거리 전송할 수 있다. 본 논문에서는 DWDM 해저 광 통신망에서 고속 대용량 트래픽을 장거리 전송하기 위한 기술을 알아보고, 영분산과 파이버의 비선형 사이에 상충문제를 해결 위한 분산관리 기법을 소개하고, 실제한국-홍콩간 해저 광통신 구간에서 분산 맵 (dispersion map)을 설계 구현하였다. Currently, Most of the Backhual and Submarine s networks are being installed using DWDM and EDFA to deal with dramatically increasing internet traffic. In a conventional type optical system, the so-called 3R repeater, fiber non- linearity was not significant factor but it is one of the most important consideration when designing new DWDM network based on the EDFAs. To minimize the signal pulse distortion in long-haul optical network, Zero-dispersion fiber is indispensable but causes the fiber non-linearity. Therefore this conflicting problems should be managed to transmit a high-speed capacity traffic. In this paper, key technologies in DWDM submarine optic network were found out for a high capacity traffic and introduced the dispersion management way for solving the conflicting problems between Zero-dispersion and non-linearity. Lastly designed a dispersion map in section between Korea and Hong-kong.

수도권 지역의 연령에 따른 전통 떡류의 이용현황

신민자,김옥선,정재홍 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.6

The purpose of this study was to investigate the consumption patterns of the Korean traditional rice cakes by general public. Self-administered questionnaires were collected from 511 residents in the Metropolitan area. Data were analysed by t-test, ANOVA and Chi-square. The result of the study can be summarized as follows: Generally, the rice cake was recognized as a "good food" in all age groups. The reasons why they prefer the rice cakes were "It tastes good" and "It has been eaten from the past." The time when they use the rice cake was mostly the holidays and events(or festivities). The female had more experiences than the male in making it. Songpyeon took the first place for them to have experiences to make. The consumption frequencies of rice cake were the more in females than in males. The higher the age, the more frequently they consumed. Injeolmi was the one which was purchased the most frequently. The place that they usually buy from was the rice cake shop around their house. The most important factor considering when they buy it was the taste. The reason why the frequency the rice cakes made for their direct consumption at home is gradually decreasing was "It is troublesome to make", and "It is complicated to make.

Low doses of ionizing radiation suppress doxorubicin-induced senescence-like phenotypes by activation of ERK1/2 and suppression of p38 kinase in MCF7 human breast cancer cells

신재식,우상혁,이형찬,홍승우,유두현,홍석일,이왕재,이명석,진영우,안성관,진동훈,박인철 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

忠南地域 觀光農業의 開發方案 Ⅰ : 觀光農業 示範農場의 經營分析 Pilot Farm Management Analysis

愼鏞仁,申東完,林栽煥,金再洪 忠南大學校 地域開發硏究所 1990 地域開發論叢 Vol.2 No.-

各種 Alcohol 濃度의 人蔘酒가 Mouse의 腦 및 心臟組織內 Catecholamine 含量에 미치는 影響

申萬鍊,全普權,閔本泓,李在鉉,金鐸 최신의학사 1985 最新醫學 Vol.28 No.10

Metallo-β-lactamase 생성 Pseudomonas aeruginosa 및 포도당 비발효그람음성간균의 검출방법에 관한 연구

신경섭,김원식,손재철,홍승복,최재운,형성민 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

연구목적: Metallo-β-lactamse (MBL)는 carbepenem를 포함하는 대부분의 β-lactam 항균제를 가수분해할 수 있으므로 MBL 생성균의 확산은 이들 균에 의한 감염의 치료에 큰 문제를 야기할 수 있다. 따라서 MBL 생성균에 의한 감염의 확산을 막기 위해 이들 균의 조기 검출이 필요하다. 저자들은 MBL 생성균을 검출할 수 있는 세 가지 방법을 PCR 방법과 비교하여 보았다. 재료 및 방법: Imipenem의 MIC가 8 μg/mL 이상인 50개의 포도당 비발효 그람음성 간균을 대상으로 EDTA-double disk synergy test (EDTA-DDS), Etest MBL, Hodge 변법을 시행하였으며 대조 검사로 IMP-1, VIM-1, VIM-2에 대한 PCR을 시행하였다. 결과: 총 50 균주 중 MBL 생성균은 Pseudomonas aeruginosa 1 균주, Alcaligenens xylosxidans 7 균주 등 8 균주(16%)가 검출되었다. 이들 균주는 모두 VIM-2 형이었으며, EDTA-DDS, Etest MBL은 PCR 방법과 100% 일치하였다. Hodge 변법은 8 MBL 생성균주 중 2 균주를 검출하지 못했다. 결론: 50 균주 중 8 균주가 MBL 생성균이었고 모두 VIM-2 형이었다. Etest MBL은 가격이 비싸 검사실에서는 EDTA-DDS가 적당할 것으로 사료된다. Purpose: Because metallo-β-lactamase (MBL) has activity to hydrolysls against most β-lactam drugs, dissemination of MBL producing bacteria may cause problems of treatment of infection by their microorganisms. Early detection or screening for MBLs will contribute to prevent further spread of resistance. Authors compared three MBL detection methods with PCR for MBLs. Materials and Methods: For 50 isolates of g1ucose nonfermentative gram negative bacilli with reduced susceptability against imipenem (MIC≥8 μg/mL), EDTA-double disk synergy (EDTA-DDS), Etest MBL and modified Hodge test were compared with PCR detection method for MBLs. Results: Among 50 isolates of glucose nonfermentative microorganisms, eight MBL producers including a Pseudomonas aeruginosa and 7 Alcaligenes xylosoxidans were detected. Those isolates were VIM-2 producer. The results of EDTA-DDS and Etest MBL completely agreed with PCR for MBL detection. Two isolates among eight MBL Producers were not detected in modified Hodge test. Conclusion: All of MBL producing bacteria were VIM-2 genotype. In conclusion, EDTA-DDS may be useful method for MBL detection in clinical laboratory due to high cost of Etest MBL strip.

입원한 정신장애자의 도박성향에 관한 연구

신광철,이길홍,이재광 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.5

The authors have tried this study to know gambling trends, to extract various factors that can be related to gambling behaviors of the mentally illed persons, and to make the development of preventive guidelines which can prevent gambling behaviors in advance. Samples were gathered from 428 psychiatric inpatients who were admitted to hospitals in Seoul and district on March 1988. Data were processed by IBM PC AT Computed using SPSS+ program and were statistically analysed by internal consistency of gambling items, correlation matrix of gambling items and chi-square test. Summary of the results were as follows: 1) Among the nine diagnostic criteria for pathological gambling, three diagnostic criteria, such as “restlessness or irritability if unable to gamble(22.2%)”,“frequent gambling of larger amounts of money or over a over a longer period of time than intended(20.6%)”,and“a need to increase the size or frequency of bets to achieve the desired excitement(20.3%)”,were more frequently experienced criteria. And all the nine criteria were experienced more frequently in males than in females. 2) 32.7% (140 persons) of the total psychiatric inpatients(428 persons), including social gambler as 13.1% and pathological gambler as 19.6%, have experienced gambling behaviors. The rate of pathological gambling in males as 26.1% was 2.46 times higher than females 10.6%. 3) Pathological gambling trends were highest in psychiatric inpatients who has grown up in the outskirts(25.7%) and the center of the small and medium city(22.4%), and higher in the outskirts of the Seoul(21.9%) and the center of the large city(21.1%), but pathological gambling trends were lowest in the group who has grown up in center of Seoul. But gambling trends were not correlated with age level, educational level, and location of the hospital. 4) Pathological gambling trends were higher in psychiatric inpatients whose body types dysplastic(52.2%), while lower in balanced body type(14.3%). 5) Pathological gambling trends were higher in psychiatric inpatients whose personality trait is antisocial(50.0%) than the other personality trait, while lower in borderline personlity (2.9%). 6) Pathological gambling trends were higher in psychiatric inpatients who have learned masturbation during middle adolescent period and who had difficulties in premorbid sexual adjustment. 7) Gambling trends were higher in psychiatric inpatients who drinks alcohol habitually (Social drinking, 36.4%; alcohol addiction, 32.7%). Among them, gambling trends were higher in patients who drink alcohol hedonically(30.1%). However gambling trends were lower in patients who do not drink(9.7%).

<사씨남정기>의 선악 구도

신재홍 고려대학교 민족문화연구원 한국문학연구소 2001 한국문학연구 Vol.- No.2

This paper is a study of the ethical theme in the Korean classical novel SassiNamjeongGi written by Kim Man-Jung(1637∼1692). The previous discussions on this work were inclined to the realistic approach to the background of the work. In the process of discussion, the primary property of the work seems to be neglected. According to our study, the writer's basic intention is the examination of the good and evil nature of human beings in the ethical view of the oriental middle ages. Therefore this work would belong to the category of the ethical novel which examine the human beings' nature. The story of this work is developed with three directions. Namely, there is a way of the good on which habe been stepped by SaJeong-Ok, the first wipe of Yu Yeon-Su, a way of the evil stepped by Gyo Chae-Ran, the concubine of Yu Yeon-Su, who would become his second wipe later, and a way from the evil to the good stepped by Yu Yeon-Su. Each characters in three directions are the typical figures of the human beings' nature in the oriental middle ages. The action and reaction, the connection and opposition between the good and evil have been described finely and dramatically. SassiNamjeongGI were praised as an excellent work among the Hangeul novels in its day. The praise have a valid reason of the generality of theme and the high level of figuration. Above all this work is a ideal and ethical literary work. Hence the criteria for this work would be the writer's sincerity with which examine the good and evil of human beings. From this point of view, SassiNamjeongGi is one of masterpieces among the Korean classical novels, which examine the human beings' nature with sincerity and figurate the characters with fineness.