맥주박 바이오차 처리가 암모니아 배출량에 미치는 영향 평가

이재한(Jae-Han Lee),최하연(Ha-Yeon Choi),박성용(Seong-yong Park),천진혁(Jin-Hyuk Chun),강윤구(Yun-Gu Kang),윤여욱(Yeo-Uk Yun),이기범(Gibum Yi),오택근(Taek-Keun Oh) 한국토양비료학회 2021 한국토양비료학회 학술발표회 초록집 Vol.2021 No.11

암모니아(NH₃)는 대기 중 황산화물(SOx), 질소산화물(NOx) 등과 반응하여 황산암모늄, 질산암모늄과 같은 2차 미세먼지를 생성하는 주요 미세먼지 원인물질이다. 2017년 기준 국내에서 NH₃는 총 308,298 ton year<SUP>-1</SUP> 배출되었으며, 농업부문에서 약 79.3%의 비율로 가장 많이 배출되었다. 토양에 질소질 비료의 시용 시 발생되는 NH₃의 대기로 배출은 토양 내 질소 손실의 원인이 되기도 한다. 바이오차는 넓은 표면적과 표면의 작용기를 가지고 있어 토양 내에서 중금속이나 오염물질 등을 제거하는 특징이 있다. 본 연구는 다양한 온도에서 열분해된 맥주박 바이오차 처리가 NH₃ 배출량에 미치는 영향을 평가하기 위해 실시하였다. 바이오차는 충분히 건조된 맥주박을 300, 500, 700°C에서 각각 1시간 동안 열분해하여 제조하였다. 각 바이오차를 토양에 3% (w w<SUP>-1</SUP>)씩 혼합 후 NH₃ 포집을 위해 자체 제작한 아크릴컬럼에 작토층 높이인 20 cm 까지 토양을 채웠으며, 토양 수분함량은 20%로 조절하였다. 질소질 비료는 작물별 시비처방기준의 배추를 기준으로 요소를 각 처리구에 320 kg ha<SUP>-1</SUP>로 처리하였다. NH₃의 포집은 요소 처리 후부터 약 30일간 실험실 내에서 실시하였다. 배출되는 NH₃는 0.05 N H₂SO₄으로 포집하여, Indophenol blue method로 비색정량하였다. 그 결과, 시험 기간 중 총 NH₃ 배출량은 BB<SUB>700</SUB>처리구가 약 11.83 kg ha<SUP>-1</SUP>로 가장 높게 나타났다. BB<SUB>300</SUB>처리구의 총배출량은 약 7.34 kg ha<SUP>-1</SUP>로 가장 낮았으며, N처리구에 비해 약 30.5% 저감 되었다. BB<SUB>700</SUB>처리구는 N처리구에 비해 NH₃ 총배출량이 약 12% 증가하였는데, 이는 바이오차의 높은 pH에 의한 alkali effect로 판단된다. 실험 후 토양 분석 결과, 모든 바이오차 처리구에서 pH, EC, Av. P₂O<SUB>5</SUB>, T-C, T-N 등이 증가하였다. 특히, BB<SUB>700</SUB>처리구는 처리된 바이오차의 높은 탄소 함량으로 인하여, 토양 내 탄소 함량이 약 3.44%로 가장 높았다. 따라서, 비교적 낮은 온도에서 열분해한 BB<SUB>300</SUB>의 토양 처리가 농경지에서 배출되는 NH₃를 감축할 수 있으며, 본 연구 결과는 실제 농경지에서 배출되는 NH₃를 감축하기 위한 기초자료로 사용될 수 있을 것으로 판단된다.

마이크로 콘트롤러를 사용한 극배치 PID 자기동조기의 구성

하재해,추연규,임영도,최부귀 동아대학교 공과대학 부설 한국자원개발연구소 1993 硏究報告 Vol.17 No.2

In this paper, a speed controller using one-chip microcontroller is implemented and applied to a DC Servo Motor. Adaptive control is applied to a system for which a priori knowledge to its mathematical model is insufficient, on the basis of input and output data an apropriate controller is constructed through which the system input is synthesized. The pole-placement PID self tuning control algorithms as a control algorithm is used to research the performance of the controller through experiments.

식도를 포함한 전위장관을 침범한 전이성 악성 흑색종 1예

최정영,김준기,신성하,고영춘,조상욱,서강석,강명원,임연근,여향순,김경수 全南大醫大 2001 전남의대학술지 Vol.37 No.1

국내에서 발생하고 있는 사람 브루셀라증의 임상적 특성

김연숙,최영실,오원섭,권기태,이혁,이선희,손준성,김신우,장현하,정숙인,고관수,박미연,백경란,송재훈 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

배경 : 2002년 내국인에서의 첫 사람 브루셀라증의 증례 보고 이후 국내에서 사람 브루셀라증이 빠른 증가를 보이고 있다. 이에 연구자들은 국내에서 발생하는 브루셀라증의 임상적 특성을 연구하고자 하였다. 방법 : 2003년부터 2006년 7월까지 7개 대학병원에 내원한 환자들을 대상으로 WHO의 진단기준에 의하여 확진된 브루셀라증 환자들의 의무기록을 후향적으로 조사분석 하였다. 배양된 균은 삼성서울병원에 의뢰하여 16S rRNA 유전자 분석방법을 이용하여 종을 확인하였고, 표준시험관응집검사는 국립보건원에 의뢰하여 시행하였다. 결과 : 34예의 환자들이 브루셀라증으로 확진되었고, 31명이 남자였다. 혈액에서 B. abortus가 증명된 경우가 8예, 표준시험관 응집검사상 의미 있는 항체가 상승을 보인경우가 26예이었다. 환자들은 대부분 축산업관련 종사자였고, 추후 브루셀라증으로 확진된 소와의 직접 접촉을 통해 감염된 것으로 여겨진다. 국내 사람 브루셀라증의 가장 흔한 증상은 발열이었고, 위장관계 합병증이 가장 흔히 동반되었다. 치료로는 doxycycline/rifampicin이 24예의 환자에서 doxycycline/aminoglycoside (streptomycin 혹은 gentamicin)이 10예의 환자에서 6주 이상의 기간 동안 투여되었다. 치료에 반응을 보이지 않는 환자는 없었고, 재발이 1예 있었다. 결론 : 국내에서 발생하는 사람 브루셀라증의 원인균으로 현재까지 분리 동정된 것은 B. abortus이며, 소 브루셀라증의 증가로 인해 인체감염사례가 함께 증가하는 것으로 사료된다. 위험 직업군 종사자들에서 감염된 소와의 직접 접촉을 통해 주로 발생하고 있는 브루셀라증의 임상종상은 국외에서 발생하는 경우와 유사한 양상을 보이고 있으며 치료에 대한 반응은 비교적 좋은 편이긴 하나, 추후 보다 많은 환자들을 대상으로 한 연구가 필요하리라 사료된다. 사람 브루셀라증의 조절을 위해서는 소 브루셀라증의 조절이 필수적이며, 브루셀라증의 조절에 성공한 선진국의 사례를 적극적으로 도입해야 할 것으로 생각된다. Background : Since the first Korean case of human brucellosis was reported in 2002, the incidence of human brucellosis has been rapidly increasing. We performed this study to evaluate the clinical characteristics of human brucellosis occurring in South Korea. Materials and Methods : Demographic features, clinical manifestations, and therapeutic outcomes of cases confirmed as brucellosis according to WHO diagnostic criteria were evaluated. Species of Brucella isolates were identified with 16S rRNA gene sequencing method. Serologic diagnosis was performed with standard tube agglutination test (STA). Results : A total of 34 cases were enrolled from 2003 to 2006 in six hospitals. Eight cases were confirmed with the isolation of Brucella from blood and 26 cases were confirmed with titer of antibodies ≥1:160 by STA. Male (31) was predominant and most of the patients were cattle farmers. The most common transmission route was direct contact with infected or their cattle byproducts. Fever was the most prominent symptom and fatigue followed. Gastrointestinal and musculoskeletal systems were commonly complicated. Patients were treated with doxycycline and rifampin or aminoglycosides for more than 6 weeks. All patients responded well to the therapeutic regimens, but one patient relapsed 10 months after treatment. Conclusion : Brucellosis occurring in Korea has been caused by B. abortus, whose preferred natural host animals are cattle. Korean patients were infected through direct contact with bovine brucellosis. Clinical manifestations of brucellosis were similar to those occurring worldwide. Therapeutic outcomes were good among patients with brucellosis in Korea. In order to prevent human brucellosis, measures to eradicate bovine brucellosis should precede.

비만을 동반한 위암 환자에서 두 병을 같이 수술 치료한 경험(증례)

최경현,윤기영,문형환,신연명,서경원,안수미,송윤미,석정희,정경연,이은하 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.3

Obesity is growing problem in Korea. We had a case of bariatric surgery during gastric cancer operation. Patient was 29 year old Korean lady with early gastric cancer located in the lesser curvature side of the middle 1/3 of the stomach. Her body weight was 89 kg, height 163 cm, and thus body mass index was 33.5 kg/m2. Preoperative blood pressure was 130/90 mmHg, hemoglobin 12.9 g/dL, total lymphocyte count 3,280/mL, serum albumin 4.3 g/dL, CEA 1.1 ng/mL, CA 19-9 9.1 ng/mL, and alpha fetoprotein 2 ng/mL. Another associated disease was right thyroid follicular neoplasm. The chest X-ray was normal. After IM injection of 2,500 units of heparin 30 minutes before the induction of general anesthesia, she received curative subtotal gastrectomy and Roux en Y gastrojejunostomy when a malabsorption loop of 120 cm jejunum was incorporated between the Treitz ligament and the end to side jejuno-jejunostmy site at May 29th 2006. Her postoperative course was uneventful except a minor wound seroma and the elevations of serum amylase(up to 4 folds) and lipase(up to 2 folds). She lost her body weight 9 kg in 5th, 21 kg in 7th, and 27 kg in 10th postoperative months to became 61.5 kg. On follow up exams in July 2008, she gained 4 kg to overcome her weakness and fatigue. From the above result, the incorporation of a malabsorption loop during reconstruction phase of gastric cancer operation was a good option for obese gastric cancer patients especially in cases of early gastric cancer.

Suppression of lung inflammation by the methanol extract of <i>Spilanthes acmella</i> Murray is related to differential regulation of NF-κB and Nrf2

Kim, Kyun Ha,Kim, Eun Jung,Kwun, Min Jung,Lee, Ji Yeon,Bach, Tran The,Eum, Sang Mi,Choi, Jun Yong,Cho, Sayeon,Kim, Sang-Jun,Jeong, Seung-Il,Joo, Myungsoo Elsevier 2018 Journal of Ethnopharmacology Vol.217 No.-

<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P>Although <I>Spilanthes acmella</I> has been used to relieve inflammation, fever, pain, or infection in traditional Asian medicine, experimental evidence supporting these functions is scarce. Here, we examined an anti-inflammatory function and a possible underlying mechanism of <I>S. acmella</I> Murray (SAM).</P> <P><B>Materials and method</B></P> <P>The methanol extract of SAM was fingerprinted by HPLC. C57BL/6 mice were administered with a single intratracheal (i.t.) LPS and 2 h later with a single i.t. SAM. The effect of SAM on lung inflammation was assessed by histology, semi-quantitative RT-PCR, and MPO assay of lung tissue. The effects of SAM on a pro-inflammatory factor NF-κB and an anti-inflammatory factor Nrf2 were analyzed by immunoblotting of nuclear proteins and by semi-quantitative RT-PCR analysis of mRNA of the genes governed by these transcription factors. V5-Nrf2 was precipitated by an anti-V5 antibody and the ubiquitinated V5-Nrf2 was revealed by immunoblotting of HA-tagged ubiquitin.</P> <P><B>Results</B></P> <P>The i.t. SAM robustly diminished a neutrophilic lung inflammation induced by i.t. LPS treatment of mice. In RAW 264.7 cells, SAM suppressed the nuclear localization of NF-κB and the expression of NF-κB-dependent cytokine genes. SAM increased the level of Nrf2 in the nucleus and the expression of Nrf2-dependent genes while suppressing ubiquitination of Nrf2.</P> <P><B>Conclusion</B></P> <P>Our results suggest that SAM can suppress a neutrophilic inflammation in mouse lungs, which is associated with suppressed NF-κB and activated Nrf2. Our results provide experimental evidence supporting the anti-inflammatory function of <I>S. acmella</I>.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Ethylenediaminetetraacetic acid (EDTA)항응고제에 의한 Mean Corpusculaar Volume(MCV)변화

홍승민,박영진,최하여,임진,정옥연,이호준,이호영,장숙진,문대수 조선의대 1996 The Medical Journal of Chosun University Vol.21 No.2

Ethylenediaminetetraacetic acid(EDTA)항응고제에 의한 Mean Corpuscular Volume(MCV)변화

홍승민,박영진,최하영,임진,정옥연,이호준,이호영,장숙진,문대수 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.2

It is essential that we put the anticoagulant into the blood specimen of the CBC test. However, the value of some parameters in the CBC test can be changed whenever using some anticoagulants. EDTA anticoagulant is mostly used these days. There are 3 kinds of EDTA, that is K_(2-), K_(3-), and Na_(2-) EDTA. The author studies how many effects each the EDTA gets into the true value of blood specimen for MCV of RBCs. Especially we compared the MCV effect of different concentrations of 3 EDTA anticoagulants on the bias with time and instrument. Some results are gotten. In our results, K_(3-)EDTA anticoagulant shows more satisfactory MCV value than K_(2) or Na_(2-)EDTA. On using concentration of EDTA, 1.5㎎ EDTA/㎖ of blood show more satisfactory MCV value than 7.5㎎ EDTA/㎖ of blood. However, the choice of anticoagulant may be dependant on the different instruments.

The Effect of Horticulture Therapy Program for Behavior Problems and Subjective Mood and Energy of Childhood Cancer Patients

Yeon Ha Choi,Sang Mi Lee,Gyung Mee Gim,Seon Hee Jeong,Sook Lee 한국인간·식물·환경학회 2017 인간식물환경학회지 Vol.20 No.6

This study was conducted to apply a horticultural therapy program to childhood cancer patients, to analyze their psychosocial changes and thus to examine its possibility as a way to manage the recovery of childhood cancer patients. This study was conducted on patients who were diagnosed with childhood cancer, and they were divided into the experimental group (8 patients who visited H association offices located in 3 cities and participated in the horticultural program) and the control group (5 patients who did not participate in the program). The homogeneity between the two groups before conducting the program was compared, and there was no statistically significant difference, which proved that the two groups were homogeneous. After conducting the program, the score of social immaturity, one of the sub-categories of problem behaviors, of the experimental group was statistically significantly lower than that of the control group (p=.019). Changes before and after the program within the groups were compared, and the somatization score (p=.039) of the experimental group (p=.039) after the program was 1.13, down from 2.13 before the program. The social immaturity score (p=.017) of the experimental group after the program was 1.00, down from 2.63 before the program, and the attention problem score (p=.026) of the group after the program was 1.25, down from 2.50 before the program. The total problem behavior score (p=.034) of the experimental group after the program was 9.63, down from 18.50 before the program, showing a statistically significant decrease. The cumulative subjective mood score (p=.000) of the experimental group throughout the entire sessions after the program was 7.82, up from 6.99 before the program, showing a statistically significant increase. Therefore, it can be concluded that the horticultural therapy program developed based on the ‘relaxationsocial support-cognitive behavior’ strategy was effective to reduce the negative psychosocial state of childhood cancer patients.

Effect of nicotine exposure on the maturation of reproductive organ and bone growth during the puberty in immature female rats

( Ha Yeon Choi ),( Do Yeon Kim ),( Seul Lee ),( Jaesook Roh ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-

Objective: The cigarette smoking has increased markedly in recent years, and considerable proportion of smokers are adolescents and young adults. As the number of adolescent cigarette consumers increases, it is important to educate the public on the potential dangers of nicotine exposure during development and improve toxicity surveillance. A number of animal and clinical studies suggest that nicotine exposure interfered with spermatogenesis and steroidogenesis in the testis. In addition, evidence from meta-analysis has raised concern that high chronic exposure of nicotine has unfavorable effects on the ovary as well as bone, i.e, induction of oocyte apoptosis and osteoporosis. Thus, it is conceivable that nicotine exposure during peri-pubertal period might be disrupting sexual maturation and bone growth. Here, we investigated the effects of high nicotine exposure during the puberty using immature female SD rat. Methods: Twenty-two days old female SD rats were divided into control and nicotine groups injected with nicotine 2 mg/kg/day for 4 weeks (N= 3/group). Results: Nicotine exposure had no effect on the body weight and amount of food intake. Onset of vaginal opening was similar in both group, but the body weight at the time of vaginal opening was heavier in nicotine group than the control. We evaluated approximately 3 estrus cycles and noted one regular cycle in two animals among the control, but none in the nicotine group. However, ovarian and uterine weights or lengths were comparable between groups. On the other hand, rats exposed to nicotine had slightly lighter femur as compared with the control. Conclusion: These results suggest that nicotine exposure possibly delayed sexual maturation and long bone growth in peri-pubertal rats. [This work supported by grants from KRF-2019R1F1A1058573]