동씨침을 시술한 안면경련의 치험 3례

김태우,김효은,조명제,강영화,이재동,Kim, Tae-woo,Kim, Hyo-eun,Cho, Myung-je,Kang, Young-hwa,Lee, Jae-dong 대한침구의학회 2003 대한침구의학회지 Vol.20 No.1

Objective : Hemifacial spasm is painless uncommon disorder characterized by involuntary paroxysmal movement of one side of face, In this study, the effect of Dong-Si acupuncture therapy for three patients with hemifacial spasm were evaluated. Methods : We have treated them with acupuncture treatment of Dong-Si acupuncture points(Cheuk-Samni and Cheuk-ha-Samni) and evaluated the effect by Scott's scale, For the first two weeks, it was dine once a day, and after two weeks, the other day. Results : Before therapy, the grades of spasm intensity classified by Scott's description in three cases were 3. And after Dong-Si acupuncture therapy, the grade of one case was 0, and two cases were 1. Conclusion : The Dong-Si acupuncture therapy for hemifacial spasm was effective and will be attempted to the patients with it.

레니텍^(�) 정(말레인산 에날라프릴, 10mg)에 대한 에나레이스 정의 생물학적 동등성

조성희,하용화,홍성제,서성훈,류재환,김동현,이경태 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3

The purpose of present study was to evaluate the bioequivalence of two enalapril maleate tablets, Renitec^(™) (MSD Korea Ltd.) and Enalace ^(™) (Welfide Korea Ltd.), according to the guidelines of Korea Food Drug Administration (KFDA). Twenty-four normal male volunteers, 22.33±2.55 year in age and 66.54±8.30 ㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After two tablets containint 10 ㎎ of enal-april maleate per tablet wre orally administered, blood was taken at predetermined time intervals and concentrations of enalapril in plasma were determined using LC-MS-MS. Pharmacokinetic parameters such as AUC_(t), C_(max) and T_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t) and C_(max) untransformed T_(max). There were no sequence effects between two formulations in these parameters. The 90% confidence intervals for the log transfored data were acceptance range of log0.8 to log1.25(e.g., log1.02∼log1.14 and log1.03∼log1.19 for AUC_(t) and C_(max), respectively). The major parameters, AUC_(t) and C_(max), met the criteria of KDFA for bioequivalence indicating that Enalace^(™) tablet is bioequivalent to Renitec^(™) tablet.

우리나라 연 작업자들의 연폭로 수준에 관한 연구

조인숙,김용배,리갑수,김화성,황규윤,장봉기,이성수,안규동,이병국 순천향대학교 산업의학연구소 1999 순천향산업의학 Vol.5 No.1

To assess lead exposure in Korean lead workers, 2047 male lead workers in 17 lead using industries were studied. Study population were divided into 4 occupation categories such as storage battery industry(type 1), secondary smelting and litharge making industries(type 2), PVC stabilizer making industries(type 3) and primary smelting and related industries (type 4). Blood lead and zinc protoporphyrin in whole blood (ZPP) were selected as an index of lead exposure. Hemoglobin and hematocrit were also checked. Fifteen questionnaires of lead related symptoms were provided to all lead workers and filled up by themselves and confirmed by medical doctors. Personal variables such as age, work duration, smoking and drinking habits were also collected. the results were as follows. 1) The mean blood lead, ZPP and hemoglobin of all lead workers were 29.7+12.6㎍/100㎖, 56.5+38.6㎍/100㎖ and 14.6+1.3 gm/100㎖ respectively. 2) While the mean blood lead of lead workers in type 1, 2, 3 and 4 were 28.6+11.6㎍/100㎖, 46.2+15.3 ㎍/100㎖, 46.8+12.1 ㎍/100㎖ and 26.2+10.2 ㎍/100㎖, the mean value of blood ZPP of those workers were 53.6+32.6 ㎍/100㎖, 108.1+87.1 ㎍/100㎖, 98.9 +54.9 ㎍/100㎖ and 45.8+19.5 ㎍/100㎖ respectively. 3) The percents of lead workers whose blood lead were 40-59.9 ㎍/100㎖ and over 60.0 ㎍/100㎖ were 17.6% and 2.1% respectively. Those whose blood lead were 40-59.9 ㎍/100㎖ and over 60.0 ㎍/100㎖ were 15.18% and 0.96% for type 1, 43.59% and 21.79% for type 2, 58.89% and 12.22% for type 3 and 11.82% and 0% for type 4 respectively. 4) The percents of lead workers whose blood ZPP were 100-149 ㎍/100㎖ and over 150 ㎍/100㎖ were 5.17% and 3.17% respectively. Those whose blood ZPP were 100-149 ㎍/100㎖ and over 150 ㎍/100㎖ were 4.66% and 2.04% for type 1, 10.26% and 25.64% for type 2, 23.33 and 13.33% for type 3 and 1.28% and 0.32% for type 4 respectively. 5) The prevalence rate of smoking and drinking of male lead workers in all lead industries were 70.8% and 71.0% respectively. While the mean blood lead of smoker, non-smoker, drinker and non-drinker were 30.24+12.58 ㎍/100㎖, 28.35+12.7 ㎍/100㎖, 30.24 ㎍/100㎖ and 28.74 ㎍/100㎖ respectively, the mean blood ZPP of those were 55.7+37.1 ㎍/100㎖, 58.4+41.9 ㎍/100㎖, 55.7+36.5 ㎍/100㎖ and 58.4+43.3 ㎍/100㎖ respectively. 6) While the correlation of hematocrit corrected blood lead with other lead exposure variables were higher than non-corrected blood lead, the correlation of log-transformed ZPP with other lead exposure variables were higher than non-corrected blood ZPP. 7) The most frequent symptom among 15 lead related symptom questionnaires was 'general fatigue' and the next one was 'feeling irritated with disturbance' and least one was 'acute abdominal pain around the umbilicus' in all lead workers. The overall prevalence of lead workers in storage battery industry and primary lead smelting industry were higher than secondary lead smelting and PVC stabilizer making industries. 8) The prevalence of lead related symptoms was a little higher in 13 symptoms in smokers than non-smokers and in 14 symptoms in drinker than non-drinkers, but they were not statistically significant. 9) Stepwise multiple regression analysis, using overall symptom prevalence as a dependent variable blood lead, blood ZPP, hemoglobin, age, work duration, drinking and smoking habits as independent variables, indicated that smoking habit, drinking habit and age were significantly contributed to the dependent variable. 10) The slopes of simple linear regression of blood lead (independent variable) with blood ZPP and log-transformed ZPP as dependent variable were different among 4 types of lead industries. The slope of lead industries with low mean blood lead were less steeper than those with high mean blood lead.

水稻 直播栽培에 關한 硏究 : (Ⅰ)栽培方法이 節間長에 미치는 影響

趙東三,許華永,朴成圭,尹洪載,金泰秀 충북대학교 농업과학기술연구소 1989 農業科學硏究 Vol.7 No.2

Data on internode length obtained from cultural experiments on seeding date, seeding method and seeding rate in direct seeding cultivation of rice in 1989 were analyzed to investigate the rela-tionship between internode length and lodging. In general, internode length appeared to be longer in earlier plantings except May 11 planting which had longer internode than those of other plantings. Seeding in flooded condition as well as higher seeding rate resulted in longer internode length. Culm diameter leas greater in the order of April 21 > May 11 > May 21 > May 1 plantings, and drilled in upland condition > broadcasting in upland condition > seeding in flooded condition, while the higher the seeding rate, the greater the culm diameter was. Except seeding in upland condition and seething rate of 3kg/10a which showed lodging degree of 1, all other treatments resulted in severe lodging with degree of 7.

음향 방출을 이용한 저어널 베어링의 조기 파손 감지(Ⅲ) - 저어널 베어링 AE 진단 시스템 개발

조용상,권오양,정민화,윤동진 韓國非破壞檢査學會 1996 한국비파괴검사학회지 Vol.16 No.3

회전 기계의 저어널 베어링 상태를 음향 방출(AE) 기술을 활용하여 감시하기 위한 진단 시스템을 개발하였다. AE 기술은 베어링 시스템에 있어서 비정상 상태를 탐지하기 위하여 이용된다. 모의 저어널 베어링 시스템을 이용한 실험과 실제 발전 설비에 대한 적용 시험의 결과로부터 AE 신호 파라메타 중에서 rms voltage가 가장 유효한 것으로 판명되었으며, 이러한 연구 결과를 토대로 하여 진단 시스템의 알고리즘과 판단 기준들이 설정되었다. 베어링 진단 시스템은 AE 센서 및 전치앰프로 구성된 신호 감지부, AE rms voltage를 측정하기 위한 rms-to-DC 변환 회로부로 구성된 신호 처리부, A/D 변환기를 이용하여 rms voltage 신호를 PC에 연결해 주는 인터페이스부, 베어링 상태 보기와 진단 프로그램을 포함하는 그래픽 디스플레이 및 소프트웨어부의 4부분으로 구성된다. For the condition monitoring of the journal bearing in rotating machinery, a system for their diagnosis by acoustic emission(AE) was developed. AE has been used to detect abnormal conditions in the bearing system. It was found from the field application study as well as the laboratory experiment using a simulated journal bearing system that AE RMS voltage was the most efficient parameter for the purpose of current study. Based on the above results, algorithms and judgement criteria for the diagnosis system was established. The system is composed of four parts as follows: the sensing part including AE sensor and preamplifier, the signal processing part for RMS-to-DC conversion to measure AE rms voltage, the interface part for transferring RMS voltage data into PC using A/D converter, and the software part including the graphic display of bearing condition and the diagnosis program.

체내동태 연구를 위한 혈청 중 펜톡시필린의 HPLC 정량법 개발 및 검증

조혜영,강현아,류희두,이화정,문재동,이용복 한국약제학회 2006 Journal of Pharmaceutical Investigation Vol.36 No.2

Ⅱ급 부정교합자에서 액티베이터 사용에 따른 atlas의 위치 변화에 관한 연구

조문기,이진우,차경석,정동화 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.1

본 연구는 Ⅱ급 부정교합자에서 액티베이터 사용 후, 하악골의 변화에 따른 atlas의 위치 및 형태 변화를 조사하고, 그에 따른 치료 효과를 예측해 보고자 시행하였다. 실험군으로 Ⅱ급 부정교합자로써 액티베이터 치료를 시행한 경우 1군(총30명, 남자 15, 여자 15명), 대조군들로 Ⅱ급 부정교합자로 액티베이터 치료를 받지 않고, 교정치료를 받은 2군(총 22명, 남자 12, 여자 10)과 Ⅰ급 부정교합자로 치료를 받은 환자 3군(총 22명, 남자 12, 여자 10)으로 총 세 개의 군으로 나누었다. 치료 전(T1), 액티베이터 사용 중지 및 치료 중간시기(T2), 치료 종료(T3)시의 측모두부방사선사진을 통해서 골격 형태 계측 및 atlas 형태 계측을 시행하였다. Ⅱ급 액티베이터 사용 결과 액티베이터를 사용하지 않은 Ⅱ급 부정교합자 군에 비해 치료 종료 시 다음과 같은 계측치 들에서 골격적인 효과가 나타났다: ramal height, body length와 effective body length는 증가하였고, ANB는 감소하였다. Overjet은 두 군 사이에서 액티베이터 사용 후 유의한 감소가 일어났으나, 치료 종료 시에는 유의한 변화가 없었다. 세 군 모두에서 FH에 대한 atlas의 시계방향 회전이 나타났으나, 실험군 1군에서 대조군 2, 3군에 비해 유의하게 atlas가 FH에 대해 시계방향 회전되는 결과가 나타났다. 1군이 3군에 비해서 atlas의 유의한 후방이동을 보인 것을 제외하고는 세 군 사이의 atlas의 전, 후방적 위치나, 형태의 크기 변화에 있어 유의한 차이점은 없었다. 위의 결과들을 고려해 볼 때 atlas의 장축의 시계방향 회전은 액티베이터의 사용 효과로 생각되며, 이는 차후 Ⅱ급 부정교합자에서 액티베이터 치료 효과를 판단하는 또 하나의 지표가 될 수 있다고 생각한다. Objective: Previous studies have reported that morphological features of the first cervical vertebra(atlas) have been associated with mandibular growth direction. The purpose of this study was to show the possible positional and morphological changes of the Atlas from activator treatment in Class Ⅱ malocclusion patients. Methods: Lateral cephalometric radiograph tracings were made at initial, middle and final stages of treatment. Angular and linear measurements of skeletal and morphological features were measured on the anatomical landmarks and reference planes. Results: The skeletal effects of activator treatment on Class Ⅱ malocclusion patients were evident on ramal height, body length, effective body length, ANB, and overjet. Clockwise rotation of the long axis of the Atlas was found in Group 1, but there was no inclination change of the Atlas in groups 2 and 3. There was no significant correlation between anterior and posterior positions of the atlas or morphological change in all groups. - except for posterior movements of the Atlas found in group 1. Conclusion: Clockwise rotation of the atlas axis resulted from activator treatment in CI Ⅱ malocclusion patients. Change in atlas axis can be thought of as an indicator for success of activator treatment.

Congo Red에 의한 이질균 세포막 단백의 변화

강동민,이상화,이유철,설성용,조동택 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.1

To study the correlation between virulence and Congo red binding capacity of Shigella, 2 invasive strains of Shigella flexneri to HeLa cell were subjected to investigate the regulation of levels of membrane proteins mediated by Congo red. Two tested strains were pigmented on the agar plate containing Congo red. Virulent S. flexneri grown in the presence of Congo red at 37℃ showed increased levels of three proteins with molecular weight of 43,000, 58,000, 63,000 daltons in the sodium lauryl sarcosinate(SLS) soluble membrane fractions and those proteins were also found in the total membrane fractions. But those proteins were not found in the SLS insoluble fractions. At 32℃ or 42℃, the protein levels in the SLS soluble fractions or SLS insoluble fractions unaffected by the presence of Congo red. The expression of these proteins was dependent to the incubation temperature.

환기정도에 따른 수술실용 신발 종류가 수술실 오염에 미치는 영향 : How to Choose Suitable Shoes?

남경동,정혜선,박영신,원진희,주미자,성화신,이지혜,이병희,조경숙,배재춘 한국의료QA학회 2000 한국의료질향상학회지 Vol.7 No.1

Background : Various types of protective footwear have been used to minimize bacterial contamination in operating rooms. In recent years, debate has arisen concerning the need for use of such protective footwear. This study was designed to provide useful data about choosing shoes most suitable for the surgical environment. Methods : Between November, 1999 and January, 2000, we performed this experimental study by comparing effect of three types of shoes (i.e., disposable shoescover, operating room-restricted shoes, and ordinary shoes) on bacterial contamination of operating rooms equipped with two different ventilation systems (i.e., high air-change, low air-change) respectively. Data were collected during two-hour sham operations in which subjects and their activities were strictly standardized. Bacterial flora were sampled from the study area floor and air colony-counts were measured. Results : In experiments involving high air-change ventilation system, there was a significant difference of floor contamination between three types of shoes, but no difference of air contamination. Under low air-change system, there was a significant difference of both floor and air contamination between three types of shoes. Conclusion : The results show that protective footwear would be unnecessary in the operating room with high air-change ventilation system, but it is important to choose suitable shoes carefully under low air-change system. Therefore, the use of outdoor shoes can be considered under high air-change system, but it would seem sensible to apply their first use in less bloody operations at the day surgery center or out-patient department to prevent transfer of body fluid into the outside environment.

효소면역측정법을 이용한 Fumonisin의 검출법 개발

손동화,한성민,임선희,이인원,조선희,강신영,이경애 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

Fumonisin에 대한 효소면역측정법(ELISA)을 개발하기 위하여 특이항체를 생산하고, 분석조건을 확립하여, 인위적으로 오염시킨 옥수수 시료의 분석을 행하였다. Fumonisin B_1 (FB_1)을 cholera toxin (CT)에 결합시킨 FB_1-CT conjugate를 면역원으로 하여 Freund's adjuvant와 함께, 또는 단독으로 두 군의 토끼에 면역하고 특이항체를 생산하였다. 항혈청을 ELISA로 분석한 결과 그 adjuvant와 함께 면역한 경우 항체역가(titer)가 높게 나타났다. 가장 높은 항체역가(1 : 16,000)를 나타낸 항혈청 및 그로부터 정제한 항체를 이용하여 간접법 및 직접법에 의한 경합적 ELISA(ciELISA 및 cdELISA)를 각각 확립하였다. 이 항체의 유사독소와의 교차반응을 ciELISA로 조사하였을 때, FB_3에 대한 교차반응율은 2%로 매우 낮았으나, FB_2에 대한 것은 179%로 다소 높았다. FB_1의 검출한계는 ciELISA에서 0.03ppb, cdELISA에서 0.3 ppb로 각각 나타났다. 실제 곡물시료의 분석에 ELISA 활용가능성을 조사하기 위하여 인위적으로 FB_1을 오염시킨 옥수수를 75% methanol로 추출하고 그 회수율을 측정한 결과, 분석이 불안정하여 회수율이 일정하게 나타나지 않았다. 그러나 방해물질의 제거를 위하여 시료추출액을 strong anion exchange(SAX) cartridge를 거쳐 세척한 다음 분석하였을 때, 3~10 ppm 범위의 FB_1 오염 옥수수에서 평균 34%(CV의 평균, 8.2%)의 안정된 회수율을 보였다. In order to develop enzyme-linked immunosorbent assay (ELISA) for fumonisins, production of specific antibodies, establishement of ELISA conditions, and quantitation of the toxin from spiked corns by ELISA were performed. Fumonisin B_1 (FB_1) conjugated to cholera toxin (CT) with or without Freund's adjuvant was subcutaneously injected into 2 groups of rabbits. When the titer of the antisera produced by each rabbit tested, higher titer was observed in case of the immunization with the adjuvant. By use of the antiserum showing the highest titer (1 : 16,000) and its purified antibodies, competitive indirect and direct ELISA's (ciELISA and cdELISA) were established, respectively. When the cross-reactivity of the antibody against fumonisin analogs was investigate by the ciELISA is was very low against B_3 (2%) but high against fumonisin B_2 (179%). The sensitivity of the ELISAs was also very high, because the detection limit for FB_1 was 0.03 ppb in ciELISA and 0.3 ppb in cdELISA. When the ELISA's were applied to the spiked corns after extraction with 75% methanol, the assay recovery of FB_1 was too unstable to assay. However, when cleanup by strong anion exchange (SAX) cartridge was introduced to remove interfering materials, the mean ELISA recovery of FB_1 from corns spiked to 3~10 ppm was found to be 34.0% and stable (mean of CV, 8.2%).