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        • 보리등겨로 제조한 간장의 맛성분 특성

          손동화,권오준,최웅규,권오진,이석일,임무혁,권광일,김성홍,정영건,Son, Dong-Hwa,Kwon, O-Jun,Choi, Ung-Kyu,Kwon, O-Jin,Lee, Suk-Il,Im, Moo-Hyeg,Kwon, Kwang-Il,Kim, Sung-Hong,Chung, Yung-Gun 한국응용생명화학회 2002 한국농화학회지 Vol.45 No.1

          본 연구는 보리등겨로 제조한 간장 맛의 특성을 찾기 위해서 수행되었다. 맛성분은 기기분석으로, 관능검사는 panel로, 그 외 통계적 처리의 방법 등을 이용하였다. 보리간장 맛성분은 유기산, 유리당 및 유리아미노산으로 분류하였으며, 이들과 관능검사 성적과의 단순상관으로 보리간장 맛의 품질을 결정하는 것은 불가능하였다. 중상관계수는 절대값의 대수 변환에서 가장 높게 나타났으며, 따라서 단계적 중회귀분석은 가장 설명력이 높으며, 표준오차가 적은 절대값의 대수 변환을 이용하여 실시하였다. 단계적 중회귀분석 결과, 보리간장 맛의 좋고 나쁨에 기여를 하는 성분은 짠맛, 구수한 맛 및 쓴맛을 내는 성분 순이었다. This study was conducted to find out optimum conditions for kanjang fermented with barley bran. The correlation between taste components and sensory evaluation score was analyzed with stepwise multiple regression analysis. It was revealed that the taste of kanjang was explained with the mix of free amino acids, free sugars and organic acids. The highest multiple correlation coefficient was obtained from absolute value transformed with logarithm. Thus, stepwise multiple regression analysis was conducted with absolute value transformed with logarithm, for which F-value was highest and standard error of estimation was lowest among the multiple regression models transformed with six variables. The stepwise multiple regression analysis showed that the taste components which most contribute to the quality of taste of kanjang fermented with barley bran was salty taste component followed by palatable taste component, and bitter taste component.

        • SCOPUSKCI등재

          수입곡물 중의 Alfatoxin $B_1$ 검출을 위한 효소면역측정법의 평가

          손동화,박애란,이인원 한국미생물 · 생명공학회 1992 한국미생물·생명공학회지 Vol.20 No.3

          곡물 중의 aflatoxin $B_1(AFB_1)$을 검출하는 방법으로서 효소면역측정법(ELISA)의 활용가능성을 평가하기 위하여, ELISA 분석치를 인위적인 오염치 및 자연오염된 시료의 HPLC 분석치와 비교하였다. 분석대상 시료로는 외국산 면실박(19점), 채종박(11점), 대두박(9점), 옥수수(3점) 등위 수입곡물을 사용하였다. 각 곡물별로 작성한 표준곡선으로부터 실제 곡물시료 중 대체로 1-100ng/g 농도의 $AFB_1$분석 가능함을 알 수 있었다. 이를 기준으로하여 표준 $AFB_1$을 인위적으로 오염시킨 시료를 ELISA로 분석 하였을 때, 1 ng/g의 저농도에서는 그 회수율이 높았으나 (평균 265) 3ng/g 이상의 농도에서는 평균 138(68-193)이었으며, 각 분석치의 상대적인 분산도(C.V)는 평균 7.0(0-22)로 매우 양호하였다한편 자연오염된 시료의 ELISA 분석치를 HPLC 분석치와 비교하였을 때, 10ng/g 이하의 시료에서는 특히 채종박의 경우 두 분석치의 간에 차이가 다소 크게 나타났으나, 그 이상의 오염시료에서는 몇몇 예외를 제외하고는 비교적 근사한 수치를 나타내었다. 특히 오염도가 심각한 면실박의 경우 HPLC 분석치에 대한 ELISA 분석치의 비율이 평균 153%로 양호하였다. 따라서 본 효소면역측정법은 10ng/g 이상의 $AFB_1$이 오염된 곡물시료의 분석에 실용화가 가능한 것으로 나타났다. In order to evaluate an enzyme-linked immunosorbent assay(ELISA) for practical use in detecting aflatoxin $B_1(AFB_1)$ from cereals, we compared $AFB_1$ concentrations of samples contaminated artificially or naturally that were quantitated by the ELISA with those spiked or quantitated by HPLC. Cotton seed meals(19 items), rape seed meals(ll), soybean meals(9), and corns(3) imported from foreign countries were used as sample cereals. The standard curves of each cereal class showed that 1-100 ng/g of $AFB_1$ from cereals could be assayed by the ELISA. When artificially contaminated cereals were assayed by ELISA, the average recovery of AFB! from samples spiked to 3 ng/g and more was 138%(68-193%), although that spiked to 1 ng/g was somewhat high(268%). The average C.V. of recovery was 7.0%(0-22.2%). When naturally contaminated cereals were assayed, the concentrations of $AFB_1$ below 10 ng/g especially from rape seed meals quantitated by ELISA were much lower than those determined by HPLC. However, the concentrations of 10 ng/g and more from samples, except a few extraordinary samples. quantitated by ELISA were similar to those determined by HPLC, especially in case of cotton seed meals whose average recovery (ELISA/HPLC) was 153%. In conclusion, the ELISA was elucidated such as a practical tool to detect $AFB_1$ of 10 ng/g and more from cereals.

        • SCIEKCI등재

          Bacillus sp. CS-17의 색소 생성조건 및 색소 농축액의 항균특성

          손동화,권오진,최웅규,정영건,Son, Dong-Hwa,Kwon, Oh-Jin,Choi, Ung-Kyu,Chung, Yung-Gun 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.2

          전통 대두발효식품으로부터 색소 생성능과 protease 활성이 가장 강한 균을 선별하여 Bacillus sp. CS-l7로 동정하였다. CS-17 균의 균체증식은 배양 24시간, protease 활성은 배양 48시간, 그리고 색소 생성은 배양 72시간 후 최대에 달하였다. Bacillus sp. CS-17은 대두분말을 1.0% 첨가한 색소 생성용 기본배지에서 가장 높은 색소 생성능을 가지는 것으로 나타났다. 배양조건이 Bacillus sp. CS-17의 색소 생성에 미치는 효과를 조사한 결과, pH 8.5에서 $37^{\circ}C$, 72시간 배양하였을 때 최적의 색소 생성을 보였다. NaCl의 첨가는 색소 생성능을 억제하는 것으로 나타났다. 이상의 결과로 Bacillus sp. CS-17의 색소 생성 최적조건은 $37^{\circ}C$, pH 8.5에서 72시간동안 배양하였을 때로 추정되었다. 그람 양성균 5주 및 그람 음성균 6주에 대하여 색소 농축액의 항균활성을 paper disc법으로 조사한 결과, B. subtilis, P. aeruginosa, S. typhimurium, E. aerogenes, B. cereus, A. hydrophila의 성장에 대한 항균효과가 인정되었으나 그 활성은 전반적으로 미약하였다. A bacterium with potent activity of pigment production and protease was isolated and identified as being Bacillus sp. CS-17. Cell growth, protease activity and pigment production of the strain reached to its maximum point after 24 hrs, 48 hrs, 72 hrs, respectively. The best pigment producing ability of Bacillus sp. CS-17 was shown on basal medium for pigment production added 1.0% soybean. The high effcient conditions for pigment production was obtained at culture of pH 8.5, $37^{\circ}C$ and 72 hours. Among the tested 5 gram positive strains and 6 gram negative strains, weak antibacterial activity of pigment concentrated extracts was appeared against growth of B. subtilis, P. aeruginosa, S. typhimurium, E. aerogenes, B. cereus, A. hydrophila.

        • KCI등재

          Nivalenol의 검출을 위한 효소면역측정법

          손동화,이향범,곽보연,김수호,권창희 한국식품위생안전성학회 1998 한국식품위생안전성학회지 Vol.13 No.2

          Nivalenol(NIV)의 검출을 위한 효소면역측정법(ELISh)을 개발하기 위하여 tetraacetyl nivalenol(Ac4-NIV)에 대한 다클론항체를 생산하고 그 조건을 확립하였다. Ac4-NIV-hemisuccinate를 bovine serum albumin에 공유결합 시킨 Ac4-NIV-HS-BSA를 Freund's adjuvant와 함께 수차례 토끼에 피하면역하였다. 가장 높은 항체가를 나타낸 항혈청으로부터 정제한 항체와 Ac4-NIV-HS-HRP conjugate를 이용하여 직접 경합 ELISA(cdELISA)를 확립하였다. 그 표준곡선으로 부터 Ac4-NIV의 검출범위는 10~5,000 ng/ml(ppb)임을 알 수 있었다. 특이항체의 Ac4-NIV과 acetyl T-2에 대한 반 응성은 각각 100, 70%였으나, NIV, deoxynivalenol, 3-acetyl deoxynivalenol, 15-acetyl deoxynivalenol, triacetyl deoxynivalenol, fusarenon-X, T-2에 대한 반응성은 0.1% 이하로 극히 미약하였다. NIV를 인위적으로 오염시킨 옥수수시료를 70% acetonitrile로 추출하고 acetylation한 다음 cdELISA를 행하였을 때, 분석의 회수율은 100, 300, 1,000 ng/g(ppb)에서 각각 108, 143, and 70%(평균, 107%)로 나타났다. To develop an enzyme-linked immunosorbent assay (ELISA) for nivalenol (NIV), we produced polyclonal antibodies against tetraacetyl nivalenol (Ac4-NIV) and established EL- ISA conditions. Ac4-NIV-hemisuccinate conjugated to bovine serum albumin (Ac4-NIV-HS-BSA) was immunized with Freund's adjuvants into rabbits subcutaneously several times. By use of the antiserum showing the highest titer and Ac4-NIV-HS-HRP conjugate, we established com- petitive direct ELISA (cdELISA). Standard curve of cdELISA showed that the detection range of Ac4-NIV was about 10- 5,000 ngiml (ppb). The cross-reactivities of the polyclonal antibody to- wards Ac4-NIV and acetyl T-2 were 100 and 70% respectively, and those towards NIV, deox- ynivalenol, 3-acetyl deoxynivalenol, 15-acetyl deoxynivalenol, triacetyl deoxynivalenol, fusarenon- X, and T-2 were less than 0.1%. When cdELISA was applied to NIV-spiked corns followed by ex- traction with 70% acetonitrile and acetylation with acetic anhydride in pyridine, the recovery rates of the Ac4-NIV were 108, 143, and 70% (average, 107%) in the levels of 100, 300, and 1,000 ng/g (ppb), respectively.

        • 식품알레르기의 화학과 생물학

          손동화,Son, Dong-Hwa 한국식품연구원 1996 食品技術 Vol.9 No.2

          식품 알레르겐은 보통의 식품단백질인데, 이는 개인에 따라서 다르지만 알레르기 증상을 일으키는 원인물질로서 작용하고 심하면 생명을 앗아가기도 한다.

        • KCI우수등재

          베트남 하노이 소재 한국 아파트 현지 거주민의 거주유형과 특성분석

          손동화,최재필,정광석 대한건축학회 2017 대한건축학회논문집 Vol.33 No.11

          As the construction industry continued to depress due to the saturation of the Korean housing market, the Korean company expanded their business to overseas markets. However, since it is the position of a cultural outsider who can not fully understand the local residential culture, the business is often performed in the same way as in Korea. H apartment buildings in Hanoi, Vietnam, have almost the same structure as a unit plan for Korean apartments. Residents living in H apartments, which are different from Vietnamese traditional houses or apartments, are experiencing differences in contrast to their residential culture. Through residents, the satisfaction of the unit plan of Korean apartment could grasp whether they satisfy or not. This will be a chance for a new culture to be created from the viewpoint of residents of Vietnam, also providing a way for Korean apartments to settle in the country with less risk. The purpose of this study is to identify the factors affecting the satisfaction level of Vietnamese households living in a Korean apartment building constructed by a Korean company in Vietnam and analyze it from a cultural point of view. For this purpose, a Post Occupancy Evaluation (P.O.E) was conducted or 63 residents in Vietnam. Data mining was conducted on the datasheet with physical and resident attributes, and the residence type and characteristics were analyzed. The factors affecting the satisfaction obtained through the analysis was to derive meaning through in-depth interviews with literature and traditional Vietnamese homes and apartments residents of Korea. As a result of the analysis, the types of residents' characteristics were analyzed for four groups. And the study analyzes the causes of dissatisfaction and satisfaction with environmental factor and spatial factor through variable analysis. Also, it is confirmed that the Vietnamese residential culture.

        • 볏짚으로 제조한 청국장의 특성

          孫東和,梁成鎬,池元大 대구산업정보대학 1998 논문집 Vol.12 No.2

          This study was conducted to investigate main odor components of Chungkook-jang fermented with organisms in rice straw. In order to search major aroma components, changes of aroma compounds and sensory score were compared for fermentation period. 30 aroma compounds were identified as a result of analysis using GC-MSD. Aroma of Chungkook-jang was leaded by 5 compounds, 2,5-dimethyl-pyrazine, 2,3-dimethyl-5- ethylpyrazine, trimethylpyrazine, tetramethylpyrazine and 3,5-diethyl-2- methyl-pyrazine. Major aroma components of Chungkook-jang on aging time were studied on the basic of sensory score. The desirable odor components were showed as 1-octen-3-ol and guaiacol. The undesirable odor components were appeared as butanoic acid and 3-methyl-butanoic acid

        • KCI등재후보
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