한우 체외수정란의 동결보존 및 핵이식에 관한 연구 : Ⅱ. 체외수저란의 동결방법이 동결.융해후 생존율에 미치는 영향 Ⅱ. Effects of Survival Rates of Frozen-Thawed Embryo Frozen Methods

정장용,박희성,홍승표,김정혁,桑潤滋,離素娟 진주산업대학교 농업기술연구소 1999 農業技術硏究所報 Vol.12 No.-

본 연구는 수정란의 동결기법을 확립하고자 배반포기에 있는 한우 체외수정란을 이용하여 유리화 및 직접이식 동결방법으로 동결을 실시하여 동결·융해후 생존성, 동해방지제의 종류, 평형 및 할구의 손상여부 등에 대한 영향을 조사한 결과는 다음과 같다. 유리화 동결방법중 GESD-VS와 EFS 용액을 동해방지제로 사용하였을 때 동결·융해 수정란의 생존율은 각각 69.8 및 37.3%로써 이들간에 유의적(P<0.05)인 차이가 있었으며, 동결기를 이용한 직접이식 동결법은 생존율이 56.0%로써 유리화 동결방법과는 유의적(P<0.05)인 차이가 없었다. 동결·융해후 생존한 수정란의 발달율은 GESD-VS용액을 사용하였을때가 53.4%로써 EFS용액을 사용하였을때의 24.0%보다는 유의적(P<0.05)으로 높게 나타났다. 1 및 2단계 평형시 단계별 생존율은 각각 11.7 및 23%로써 이들간에 유의적(P<0.05)인 차이가 없었으며, 동결·융해 수정란의 발달율도 각각 10 및 16.8%로써 평형방법간에 유의적(P<0.05)인 차이가 없었다. 신선수정란, 유리화 동결수정란 및 동결비를 이용한 직접이식 동결법으로 동결하였을 때 동결·융해 수정란의 할구수는 각각 98.7±2.3개, 69.1±2.6 및 84.2±2.7개로써 동결방법들간에 유의적(P<0.05)으로 할구수의 차이가 많았다. This study was carried out to investigate the effect of cryoprotectants, equilibration step, number of blastomeres on survival and developmental rates of bovine in vitro fertilized blastocysts(Korean native cattle) frozen/thawed by vitrification and frozen for direct transfer(1.8M ethylene glycol). The bovine oocytes were collected from 2-5mm follicles, matured for 24hrs in 5% CO_2 incubator and then fertilized with cauda epididymis sperm. Blastocyst at day 7-9 ater IVF were frozen in vitrification and frozen for direct transfer(1.8M ethylene glycol). Survival and developmental rates of frozen-thawed IVF embryos were examined. The results were as follow ; The survival rate of IVF blastocyst after vitrification in GESD-VS, EFS and 1.8M ethylene glycol solution were 69.8, 37.3 and 56.0%, respectively(P<0.05). When equilibrated in 1 step(11.7%) was similar to the result from 2 step(23.0%), and no significantly(P<0.05) different between the equilibrated steps. The number of blastomere at blastomere at blastocyst of bovine embryos frozen/thawed in fresh, vitrification and freezing for direct transfer were 98.7±2.3, 69.1±2.6 and 84.2±2.7, respectively, and were significantly(P<0.05) different between the freezing methods.

일개지역 노인의 일상생활 수행능력과 우울에 관한 연구

전소자,주영희,정경옥 김천과학대학 2011 김천과학대학 논문집 Vol.37 No.-

This study aims at finding out the descriptive relationship among Activity of Daily Living(ADL) and depression for the elderly in K city. The subject were 50, who agreed to participate in this study, the data collection was performed from July 2 to August 19, 2011. The questionnaires was composed of 51 items: 21 general characteristics, 5 for ADL, 10 for IADL and 15 for depression. Data were analysed descriptive, frequency, percentage, mean, standard deviation, t-test, ANOVA Using SPSS 18.0 for Windows. The finding of this study as follows: 84% of subjects with mild depressive symptoms that have appeared over, 96% of respondents in daily living were found to experience difficulties. From above result, Relationship among Activity of Daily Living(ADL) and depression for the elderly did not show significant differences but the state's mental health is important. Therefore psychological health status should be considered in order to improve nursing intervention for life satisfaction of the elderly.

한우 체외수정란의 동결보존 및 핵이식에 관한 연구 : Ⅲ. 융합조건 및 공핵란의 발달단계가 핵이식 수정란의 체외발달에 미치는 영향 Ⅲ. Effects of Fusion System and Developmental Stage of Donor Nuclei on In Vitro Development by NT of Bovine Embryos

정장용,박희성,桑潤滋,離泰娟 진주산업대학교 농업기술연구소 2000 農業技術硏究所報 Vol.13 No.-

The present study was conducted to improve the efficiency of nuclear transplantation with in vitro produced oocytes and embryos in Korean native cows. The optimal conditions and procedures of in vitro maturation(IVM), in vitro fertilization(IVF), in vitro culture(IVC), electrofusion and nuclear transplantation(NT) of bovine oocytes were evaluated. The optimal condition for electric fusion of injected blastomeres with enucleated oocytes was determined following NT in IVM oocytes. The higher fusion rate was achieved when single DC electricity was applied at the voltage between 1.0kV/cm, for the duration of 30∼60 μ sec to the oocytes aged at 28∼32 hours of IVM. The development in vitro to blastocyst of the fused embryos of 30 μsec, 0.5-1.0 kV/cm(5.7-4.2%) was similar to the result from the 60 μsec, 0.5-1.0ikV/cm(4.8-5.6%), and not significantly different between the electric fields. To improve the productivity of cloned embryos by NT in Korean native cows. The in vitro developmental rate of donor nuclei following NT and fusion was enhanced from 4.6%(8∼6-cell) and 2.7%(32∼64-cell). The number of blastomeres was significantly(P<0.05)reduced in the nuclear transplanted embryos(65.3±4.7), compared with the IVF embryos(112.8±6.2).

急速反復動作能力에 의한 女性의 機能에 關한 硏究

鄭淸喜,裵小心 同德女子大學校 1975 同大論叢 Vol.5 No.1

motor avidity of human extremity is of essential factor for sports The purpose of this research is of as follow. ① clarify individual differences in rapid response ability of coed ② clarify effect of extremity ability for sports The theoretical background of the research is the models suggested by ikai, mazei, kawazaki, 8 subjects were relected for the research and consisted with 4 players and 4 nonplayers. method of measuremet of extremity ability was used tapping test at the rate of 50mm a second. The results are as follow; ① table tennis players are most superior in rapid extremity ability and athletic sport players have no differences with nonplayers. ② There are me differences between men and worsen in rapid response ability of extremity. ③ There are severe differences between left and right extremity in rapid response ability except table temus players. ④ There are severe individual differences in rapid response ability ⑤ ability of noneffective extremity is in proportion to effective extermity.

2-[(4-Cyanophenyl)amino]-3-chloro-1,4-naphthalenedione(NQ-Y15)의 돌연변이원성

김봉희,정기화,유충규,창동신,이기선,전선덕,소동수,채상호,문창규 한국환경독성학회 2000 환경독성보건학회지 Vol.15 No.4

2-[(4-Cyanophenyl)amino]-3-chloro-1, 4-naphthalenedione (NQ-Y15) was asssayed for its genotoxic potential by using Salmonella typhimurium reversion assay and in vitro chromosome aberration test on Chinese hamster lung cells. In the Ames test, NQ-Y 15 induced his+ revertants of Salmonella typhimurium TA 98 and TA1537, reaching levels twice the negative control values. But, NQ-Y15 induced only his^(+) revertants of Salmonella typhimurium TA1537 more than twice the control values under the condition with metabolic activation system. In the cytogenetic test on chinese hamster lung cells, NQ-Y15 showed significant chromosomal aberrations, but the incidence was significantly reduced in the presence of metabolic activation.

2-[(4-Cyanophenyl)amino]-3-chloro-1,4-naphthalenedione (NQ-Y15)의 돌연변이원성

김봉희,정기화,유충규,창동신,이기선,전선덕,소동수,채상호,문창규 德成女子大學校 藥學硏究所 2001 藥學論文誌 Vol.12 No.1

2-[(4-Cyanophenyl)amino]-3-chloro-1, 4-naphthalenedione (NQ-Y15) was asssayed for its genotoxic potential by using Salmonella typhimurium reversion assay and in vitro chromosome aberration test on Chinese hamster lung cells. In the Ames test, NQ-Y15 induced his+revertants of Salmonella typhimurium TA 98 and TA 1537, reaching levels twice the negative control values. But, NQ-Y15 induced only his+revertants of Salmonella typhimurium TA 1537 more than twice the control values under the condition with metabolic activation system. In the cytogenetic test on chinese hamster lung cells. NQ-Y15 showed significant chromosomal aberrations, but the incidence was significantly reduced in the presence of metabolic activation.

마늘첨가가 김치의 숙성 및 품질에 미치는 영향

조현경,박소희,조재선,정청송 한국식생활문화학회 2001 韓國食生活文化學會誌 Vol.16 No.5

마늘이 김치의 숙성과 품질에 미치는 영향을 조사하고 아울러 cyclodextrin과 sorbitol의 마늘냄새 masking효과를 실험하였다. 김치 숙성 초기의 산도는 마늘 첨가량이 많을수록 대조구에 비해서 높았고 환원당 함량은 마늘 첨가량이 많을수록 높았으나 숙성말기에는 거의 비슷한 수준을 유지하였다. 미생물의 경우 숙성초기에는 마늘 첨가에 의해 성장이 촉진되었으나 이후에 그 수준을 유지한 후에는 대조구에 비해서 오히려 억제되었다. 관능 검사 결과는 온도에 관계없이 마늘을 첨가하지 않은 김치가 모든 관능적인 면에서 유의적으로 나쁘게 평가되었으며 마늘을 2∼3% 첨가한 김치가 유의적으로 가장 좋게 평가되었다. Cyclodextrin 또는 sorbitol을 1%씩 첨가한 결과 마늘맛을 masking 하는데는 효과가 없었고 관능적으로도 나쁘게 평가되었다. This study was conducted to investigate the effect of garlic on the fermentation and organoleptic quality of kimchi. For this study, changes in pH, acidity, reducing sugar content and microbial count of kimchi during fermentation were tested and sensory test of fermented kimchi was also conducted. The acidity of kimchi with garlic was increased rapidly more than control kimchi at early stage of fermentation. Though the reducing sugar content was higher in kimchi with garlic than control kimchi, but that of all samples were almost same at mature stage of fermentation. Total bacteria and lactic acid bacterial counts were higher in kimchi with garlic than control kimchi at early stage of fermentation, but those were reversed at final stage of fermentation. By the result of sensory test, garlic was contributed to taste and aroma positively at level of 2∼3g garlic to 100g of kimchi.

6年根 人蔘의 等及別 品位 및 ginsenoside 함량

조재선,조현경,박소희,정청송 한국식생활문화학회 2001 韓國食生活文化學會誌 Vol.16 No.5

This study was conducted to investigate the difference of general feature and ginsenoside content of 6 years old ginseng rooot among different grade of roots. Total weight of a 1st grade-6 years old ginseng root was 115.1g and weight, length, diameter and specific gravity of main root were 64.68g, 8.39cm, 3.31cm and 0.96, respectively. Main root of 1st grade ginseng root was larger in size and specific gravity and more heavy than that of 2nd or 3rd grade of the roots. Though crude saponin contents were not so different among the different grade of roots, but ginsenoside Rbl, Rgl and Re content were higher in 1st grade of root than that of 2nd or 3rd grade of root. Those ginsenosides were located mainly in periderm and cortex.

대학생의 우울정도 : 간호학전공과 타전공대학생을 중심으로

김지연,류나은,이소라,이지희,정윤정,주지연,황인혜 이화여자대학교 간호과학대학 2012 이화간호학회지 Vol.- No.46

Purpose: This study was designed to investigate sample’s general characteristics and compare depression degree among sample’s general characteristics and students’ major(nursing vs. other major). Methods: The subjects consisted of 318 university students. Data was collected by self-reported questionnaires, which were constructed BDI score. Data was analyzed by the SPSS/PC WIN 19.0 program. Results: The depression of nursing students and other major students was not significantly different according to BDI score. Statistically significant difference was identified among sleeping. Conclusion: Specific study focused on the practice time should be done to confirm the depression of nursing major and other majors. Detailed support programs which specifically deal with sleeping should be developed to effectively reduce the harmful effects of individual vulnerability. Therefore, prevention and management system to reduce depression degree for university students is needed.

전류 비유 모델에 의한 초등학생의 전류 개념 변화 분석

유병길,강인석,김병철,남만희,박선희,소하연,윤희정,이영아,이하룡,전병문,정승호 부산교육대학교 과학교육연구소 2000 科學敎育硏究 Vol.25 No.-

There exists a major impediment to learning electricity which may illuminate why direct presentation of scientists' ideas is too aggressive a strategy. This impediment lies in thinking that electricity is used up and in envisaging the light bulb as a consumer. This view is based on a powerful idea which summarises much of our experience; it declares that 'things get used up', 'you cannot get something for nothing', and 'intuitive thinking'. To replace this view with scientific view, we made the analogy model. It is suggested that the analogy model is more effective in teaching electricity circuits than conventional teaching method.