내관(內關)(PC6).공손(公孫)(SP4) 자침이 정상인의 자율신경계에 미치는 영향 연구

유경환,임인환,김동현,나병조,이범준,박성욱,고창남,Rheu, Kyoung-Hwan,Im, In-Hwan,Kim, Dong-Hyun,Na, Byong-Jo,Lee, Beom-Jun,Park, Seong-Uk,Ko, Chang-Nam 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.3

Objectives : This study was designed to investigate the effect of PC6 and SP4 on autonomic nerve system in healthy adults. Methods : 22 healthy adults were recruited and randomized into the study group or control group. The study group (real acupuncture group) was acupuncturcd on PC6 and SP4. The control group (sham acupuncture group) was acupunctured superficially near PC6 and SP4 but not the exact points. The subjects rested for 10 minutes, and were then acupunctured. After 15 minutes, acupuncture needles were removed and the subjects rested for another 15 minutes. Heart rate variability(HRV) was continuously measured during the study period by FM-150 (digital Holter ECG recorder). Results : In the real acupuncture group. LF(low frequency) significantly decreased during the acupuncture period(p<0.05). LF/HF ratio also had a significant decrease during acupuncture period and the decrease was maintained during the post-treatment period as compared with the pre-treatment period. In the sham acupuncture group. LF significantly increased during the acupuncture period but LF/HF ratio had no significant changes. Conclusions : The results suggest that acupuncture on PC6 and SP4 can increase parasympathetic activity and balance autonomic nervous system.

위암종에서 p53 유전자 산물에 대한 면역조직화학적 연구

류도현(Do Hyun Rheu),주영은(Young Eun Joo),김태두(Tai Du Kim),지미선(Mi Sun Ji),양대현(Dai Hyun Yang),유종선(Chong Sun Rew),박창수(Chang Soo Park),윤종만(Chong Mann Yoon) 대한내과학회 1995 대한내과학회지 Vol.48 No.4

bronchogenic carcinoma and breast cancer. Although gastric cancer is the most frequent tumor in Korea, few article was reported about the mutation of p53 gene in gastric cancer. In this study, immunohistochemical staining for the mutant p53 gene product was performed to see the relation of the mutation of p53 gene to the pathologic type and staging in the gastric cancer, and to evaluate the usefulness of the mutant p53 gene as a index of gastric cancer. Methods: The mutation of p53 gene was studied with immunohistochemical staining of paraffin section which was obtained in the surgically resected 87 gastric cancer patients. Results: 1) Positive reaction for p53 gene product was observed mainly in the nuclei of tumor cells and negative reaction was observed in the normal mucosal epithelium around cancer foci. 2) The positivity of p53 gene product was 61.2% of the gastric carcinoma overall. By the histological differentiation, the positivity was 57.7% in the well differentiated adenocarcinoma, 60% in the moderately differentiated adenocarcinoma and 72.5% in the poorly differentiated adenocarcinoma, Poorly differentiated adenocarcinoma revealed the highest positivity(p<0.05). 3) Positive cell ratio of p53 gene product in cancer nest had a increased tendency in the poorly differentiated rather than in the well differentiated adenocarcinoma. Conclusion: From the above result, the mutation of p53 gene was highly expressed in gastric cancer and positive reaction of p53 gene product was highly increased in poorly differentiated cancer cell.

Mycelial growth of Lentinula edodes in response to different mixing time period, pressure intensity and substrate porosity

Hyun You Chang,Geum Hui Seo,Yong Kuk Lee,Kyoung min Rheu 한국버섯학회 2018 버섯 Vol.22 No.1

Biological efficiency (BE), the ratio of fresh mushrooms harvested per dry substrate weight and expressed as percentage of Lentinula edodes, also known as the shiitake was identified using the Sanjo 701 strain stored in the Department of Mushroom of the Korea National College of Agriculture and Fisheries. The strains were grown in glass columns with varying level of moisture content and different length of mixing periods of 0.5,1,2, and 3 hours. The substrate was sterilized to avoid contamination using the pressure steam Autoclave Sterilizer between normal and high pressure sterilization. Results show that mycelial growth (126mm/15 days) is optimized at 55% moisture level content. Two (2) hours mixing time yields the best mycelial growth of 117mm/15 days. On the other hand, normal pressure sterilization yielded better result with mycelial growth of 96mm/15 days at 100°C sterilization as compare to 88mm /15 days sterilization at 121°C. Mycelial density is higher, 3(+++) at normal pressure sterilization as compare to 2(++) at high pressure sterilization. Further, the sawdust with mixture of 5% woodchips also increased the porosity of the substrate and yielded higher mycelial growth. Thus, at this point we have shown the importance on how to achieve optimum harvest or potential increased yield of shiitake considering its moisture content, mixing time period and substrate porosity.

PⅡ-8 Mycelial growth of Lentinula edodes in response to different mixing time period, pressure intensity and substrate porosity

Hyun You Chang,Geum Hui Seo,Yong Kuk Lee,Kyoung min Rheu 한국버섯학회 2018 버섯 Vol.22 No.2

Effect of Preoperative Fatty Degeneration of the Deltoid and the Teres Minor Muscles on the Clinical Outcome after Reverse Total Shoulder Arthroplasty

Sung-Hoon Moon,Woo-Dong Nam,Chang-Hyun Rheu,Jae-Woo Lee 대한견주관절의학회 2015 대한견주관절학회지 Vol.18 No.3

Background: To evaluate the effect of preoperative fatty degeneration of deltoid and teres minor muscles on the clinical outcome in patient with reverse total shoulder arthroplasty (RTSA). Methods: Nineteen patients with RTSA were enrolled. The mean follow-up period was 16.1 months. The fatty degeneration of three distinct parts in each deltoid and the teres minor muscle was measured using a preoperative magnetic resonance imaging. Postoperatively, the muscle strengths for forward elevation (FE), abduction (Abd), and external rotation (ER) were measured using a myometer at the last follow-up. The parameters for clinical outcome were Constant Score (CS) and Korean Shoulder Score (KSS). Results: The number of cases was 10 in group 1 and 9 in group 2. The strength of FE and Abd were significantly higher in group 1 (p<0.001 and p<0.001, respectively), and the strength of ER was not different significantly between two groups (p=0.065). For the clinical outcome, both CS and KSS were higher in group 1 (p=0.002 and p=0.002, respectively). The number of patients in group A was 11, and group B was 8. Although there was not a significant difference in terms of FE and Abd between group A and B (p=0.091, p=0.238), ER was significantly higher in group A (p=0.012). We did not find a significant difference in the clinical scores (CS, p=0.177 and KSS, p=0.238). Conclusions: These findings suggest the importance of a preoperative evaluation of the fatty degeneration of deltoid and teres minor muscles for predicting postoperative strength and clinical outcome.

위암종에서 Epidermal Growth Factor Receptor 의 발현에 관한 연구 - 면역조직화학적 염색 및 in situ Hybridization -

조정곤(Jung Kon Cho),류도현(Do Hyun Rheu),최성규(Sung Kyu Choi),유종선(Jong Sun Rew),윤종만(Chong Mann Yoon),박창수(Chang Soo Park) 대한내과학회 1996 대한내과학회지 Vol.51 No.1

Objectives: Gastric carcinoma is the leading cause of death among cancer patients in Korea. The etiologic factors of gastric carcinoma were suggested to chronic gastritis, genetic defect, food and environmental factor. Recently, the studies for oncogene and tumor marker have been actively performed to investigate the causes of gastric carcinoma. Epidermal growth factor receptor(EGFR) has homology in amino acids with c-erb B2 oncogene product. EGFR was identified in the placenta, chorionic villi and gastrointestinal mucosa. But it was more expressed in tumor cells, and it may be usedas a tumor marker. Methods: To investigate the correlation between EGFR and malignant potentiality in gastric carcinoma, we performed in situ hybridization of EGFR mRNA and immunohistochemistry of c-erb B2 and EGFR in 127 cases of formalin fixed paraffin embedded tissue sections of gastric carcinoma. Results: The positive reaction for c-erb B2, EGFR protein and EGFR mRNA was identified in the cytoplasm of tumor cells. The positive ratio was 44.196 in c-erb B2, 63.8% in EGFR, and EGFR mRNA was expressed in 72.4% of cases. The expression of c-erb B2 was not related with differentiation of cells, tumor size, invasiveness and metastasis. However the expression of EGFR and EGFR mRNA was related to the invasion of primary tumor and lymph node metastasis (p<.0.05). And expression of EGFR mRNA was increased in the advances of clinical stage. Conclusion: These above results suggest that the expression of EGFR mRNA is related with malignant potentiality of gastric carcinoma. EGFR mRNA might be used as tumor marker and prognostic factor of gastric carcinoma.

만성신부전 환자에게 한약투여 후 사구체여과율 및 빈혈 호전 1례

김동현,배은주,박성욱,윤성우,고창남,유경환,Kim, Dong-Hyun,Bae, Eun-Joo,Park, Seong-Uk,Yoon, Seong-Woo,Ko, Chang-Nam,Rheu, Kyoung-Hwan 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.2

Renal failure means irreversible damage and depression of renal function, represented by glomerular filtration rate(GFR). A renal failure patient with uremia and anemia is presented. Anemia, in this patient, appeared before uremia did. A 55 year-old male patient was treated for renal failure with Weilingtang. Improvement was observed in, not only GFR, blood urea nitrogen(BUN), and creatinine, but also in such symptoms as difficulty in urine excretion, dyspepsia and abdominal swelling. But anemia appeared during treatment, so a mixture of Dangguibuxuetang(當歸補血湯) and Xiongguitang(芎歸湯) was prescribed. After this treatment, improvement in hemoglobin, and hematocrit was observed.

화병 환자의 심학변이도 특성에 대한 임상적 연구

배은주,김동현,유경환,박성욱,윤성우,고창남,Bae, Eun-Joo,Kim, Dong-Hyun,Rheu, Kyung-Hwan,Park, Seong-Uk,Yoon, Seong-Woo,Ko, Chang-Nam 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.4

Objectives : Through spectral analysis of heart rate variability(HRV) to study autonomic nervous system dysfunction of Hwabyung, culture related disease in Korea. Methods : HRV of 18 patients diagnosed as Hwabyung was measured against 13 healthy controls. HRV was measured by SA-2000p(Mediocre, Korea) for five minutes after 5 minutes resting. Results : 1. In time domain analysis, mean pulse rate(PRT) of in Hwabyung group was significantly higher than healthy controls. Standard deviation of all normal P-P intervals(SDNN), the square root of the mean of the sum of the squares of differences between adjacent normal P-P intervals(RMS-SD) in Hwabyung group was lower than healthy controls, but not significant. 2. As for frequency domain analysis, In TP(logarithmic total power), In VLF(logarithmic very low frequency), and In LF(logarithmic low frequency )in Hwabyung group was significantly lower than healthy controls. Ln HF(logarithmic high frequency), LF/HF ratio in Hwabyung group was also lower but not significant. Conclusions : This study suggests tile activity and imbalance of cardiac autonomous nervous system in Hwabyung patient is significantly lower than healthy individuals.

배란유도제가 생쥐 미성숙난자의 성숙에 미치는 영향 및 여러 배양액내에서 생쥐 2세포기의 배아 발달에 관한 연구

이종진,양춘모,문현창,이호성,이기숙,류철희,김종덕,Lee, Jong-Jin,Yang, Chun-Mo,Moon, Hyun-Chang,Lee, Ho-Seong,Lee, Ky-Sook,Rheu, Cheul-Hee,Kim, Jong-Duk 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2

Purpose of the present study was to find the optimal ovulation induction medicine for the maturation and development of immature oocytes and culture media for 2-cell embryos in the mouse model. ICR female mouse aged 6 to 8 weeks, were stimulated with 5 IU PMSG injection. At 47 to 50 hour post-PMSG injection, ovaries were dissected out and oocytes-cumulus complexes were punctured. The oocyte-cumulus complexes were cultured in media containing various ovulation induction medicine, CC, HMG and Metrodin for 18 hours. Female ICR mice were stimulated with 5 IU PMSG and 48 hours later were injected 5 IU of hCG, then female and male mice were mated. At 48 hour post-hCG injection, oviducts were dissected out and 2-cell embryos were flushed. The 2-cell embryos were cultured in various media, Ham's F-10 media of milli-Q water $(3^{\circ})$, Ham's F-10 media of HPLC (high performance liquid chromatography, Baxter) water, Medicult media, HTF (human tubal fluid) media for 96 hours. The results were as follows. 1. When the oocytes-cumulus complexes were cultured in $10^{-9}{\mu}g/ml{\sim}10^{-8}{\mu}g/ml$ of CC, those were suppressed in meiotic maturation $(28.2{\sim}33.7%)$. Whereas the oocytes-cumulus complexes were cultured in $10^{-7}{\mu}g/ml{\sim}10^{-4}{\mu}g/ml$, these were not effected in meiotic maturation $(54.5{\sim}72.7%)$. 2. When the oocytes-cumulus complexes were cultured in $10^{-4}{\mu}g/ml{\sim}10^{-1}{\mu}g/ml$ of Metrodin, those were suppressed in meiotic maturation $(35.7{\sim}41.5%)$. Meanwhile the oocytes-cumulus complexes were cultured in $10^{-7}{\mu}g/ml{\sim}10^{-5}{\mu}g/ml$, those were not effected in meiotic maturation $(54.2{\sim}70.3%)$. 3. When the oocytes-cumulus complexes were cultured in $10^{-5}{\mu}g/ml{\sim}10^{-4}{\mu}g/ml$ of HMG, those were suppressed in meiotic maturation $(48.2{\sim}50.4%)$. As being cultured in $10^{-7}{\mu}g/ml{\sim}10^{-6}{\mu}g/ml$, increased in meiotic maturation $(75.8{\sim}80.7%)$. 4. When the 2-cell embryos were cultured in Ham's F-10 media of milli-Q water $(3^{\circ})$, Ham's F-10 media of HPLC (high performance liquid chromatograpy, Baxter) water, Medicult media, HTF (human tubal fluid) media, developmental rates to blastocyst and hatching for 96 hour were 50.0%, 45.2%, 71.5% and 95.6%, respectively.

증례(症例) : 후천성 면역 결핍증 (AIDS) 연관형 Kaposi 육종 1예

이선화 ( Sun Hwa Lee ),이민희 ( Min Hee Lee ),김상균 ( Sang Kyoon Kim ),유창우 ( Chang Woo Rheu ),백경현 ( Kyung Hyun Paeck ),정치량 ( Chi Ryang Chung ),이석 ( Seok Lee ),이흥범 ( Heung Bum Lee ) 전북대학교 의과학연구소 2004 全北醫大論文集 Vol.28 No.2

Kaposi 육종은 후천성 면역 결핍증 환자에서 가장 흔하게 발생하는 신생물이지만그 치료에 대해서는 최근까지도 명확한 기준이 정립되어 었지 않았다. 근래에는 AIDS 연관형 Kaposi 육종의 병인에 있어서 HHV-8의 감염이나 숙주의 면역 상태가 중요한 역할을 한다는 것이 밝혀지면서 nucleoside analogues reverse transcriptase inhibitor와 protease inhibitor를 병합 사용하는 적극적인 항 retrovirus 치료 (HAART)를 시도하는 경향이며, 그 외에도 다양한 방법들을 사용한 성공적인 치료 예들이 보고 되고 있다. 본 저자들은 국내에 흔하게 보고되지 않은 AIDS 연관형 Kaposi 육종의 증례를 진단하고 HAART와 foscarnet 그리고 고식적 방사선 치료를 병행하여 적극 적인 내과적 치료를 시도한 증례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. AIDS-related Kaposi`s sarcoma is one of the most common neoplasms in patients with acquired immunodeficiency syndrome. Recently, human herpesvirus-8 (HHV-8) and the immune status of the host have known to be closely related to the pathogenesis of AIDS-related Kaposi`s sarcoma. Therefore, highly active antiretroviral therapy (HAART) which includes nucleoside analogues reverse transcriptase inhibitor and protease inhibitor has been widely attempted in AIDS-related Kaposi`s sarcoma. Other successful treatment regimens including cytotoxic agents, interferon-α, thalidomide and so on have been reported. However, standard treatment option. has not been established yet. Hereby, we report a case of patient with AIDS-related Kaposi`s sarcoma who was treated by zidovudine, lamivudine and indinavir for underlying HIV infection, and intravenous foscarnet and palliative radiotherapy for local control of oropharyngeal Kaposi`s sarcoma with literature review.