Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis

Lee, Jong-Uk,Cheong, Hyun Sub,Shim, Eun-Young,Bae, Da-Jeong,Chang, Hun Soo,Uh, Soo-Taek,Kim, Young Hoon,Park, Jong-Sook,Lee, Bora,Shin, Hyoung Doo,Park, Choon-Sik BioMed Central 2017 Respiratory research Vol.18 No.-

<P><B>Background</B></P><P>Idiopathic pulmonary fibrosis (IPF) is characterized by the complex interaction of cells involved in chronic inflammation and fibrosis. Global gene expression of a homogenous cell population will identify novel candidate genes.</P><P><B>Methods</B></P><P>Gene expression of fibroblasts derived from lung tissues (8 IPF and 4 controls) was profiled, and ontology and functional pathway were analyzed in the genes exhibiting >2 absolute fold changes with <I>p</I>-values < 0.05. <I>CCL8</I> mRNA and protein levels were quantified using real-time PCR and ELISA. <I>CCL8</I> localization was evaluated by immunofluorescence staining.</P><P><B>Results</B></P><P>One hundred seventy eight genes differentially expressed and 15 genes exhibited >10-fold change. Among them, 13 were novel in relation with IPF. <I>CCL8</I> expression was 22.8-fold higher in IPF fibroblasts. The levels of <I>CCL8</I> mRNA and protein were 3 and 9-fold higher in 14 IPF fibroblasts than those in 10 control fibroblasts by real-time PCR and ELISA (<I>p</I> = 0.022 and <I>p</I> = 0.026, respectively). The <I>CCL8</I> concentrations in BAL fluid was significantly higher in 86 patients with IPF than those in 41 controls, and other interstitial lung diseases including non-specific interstitial pneumonia (<I>n</I> = 22), hypersensitivity pneumonitis (<I>n</I> = 20) and sarcoidosis (<I>n</I> = 19) (<I>p</I> < 0.005, respectively). Cut-off values of 2.29 pg/mL and 0.43 pg/mL possessed 80.2 and 70.7% accuracy for the discrimination of IPF from NC and the other lung diseases, respectively. IPF subjects with <I>CCL8</I> levels >28.61 pg/mL showed shorter survival compared to those with lower levels (<I>p</I> = 0.012). <I>CCL8</I> was expressed by α-SMA-positive cells in the interstitium of IPF.</P><P><B>Conclusions</B></P><P>Transcriptome analysis identified several novel IPF-related genes. Among them, <I>CCL8</I> is a candidate molecule for the differential diagnosis and prediction of survival.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s12931-016-0493-6) contains supplementary material, which is available to authorized users.</P>

심혈관계 질환으로 관상질환 집중치료실에 입원한 환자들의 혜후 측정을 위한 APACHEⅢ점수 체계의 임상적 유용성

이공섭,이창선,최현주,홍종서,강지향,김도형,이민영,오인균,김병엽,김상억,김학찬,한승혜,이재용,지영우,안경주 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

소아에서 발생한 요추 추간판 탈출증

정창훈(Chang Hoon Jeong),이기행(Kee Haeng Lee),김윤수(Youn Soo Kim),문찬웅(Chan Woong Moon),박일중(Il Jung Park),정상룡(Sang Lyong Jung),김형민(Hyoung Min Kim) 대한정형외과학회 2005 대한정형외과학회지 Vol.40 No.2

요추 추간판 탈출증은 추간판의 퇴행성 변화가 있는 30-40대에 흔하게 발생한다. 그러나, 추간판의 퇴행성 변화가 없는 소아(12세 이하)에서는 매우 드물며 그 임상증상 및 치료 방법 역시 성인과 차이가 있다. 저자들은 소아에서 발생한 요추 추간판 탈출증 5예를 경험하였기에 이에 대해 분석, 보고하는 바이다. Lumbar intervertebal disc herniation is common in the fourth to fifth decades because the intervertebral disc undergoes degenerative change. However juvenile lumbar intervertebal disc herniation (under 12 years old) is rare because there is no degenerative change, and the clinical symptoms and treatments are different from those of adults. Herein, our experience of five juvenile lumbar intervertebal disc herniation cases are analyzed and reported.

근전도 시스템을 이용한 테니스 퍼스트와 세컨드 서비스의 생체역학적 연구-남ㆍ여 고교 테니스선수를 중심으로 : 남ㆍ여 고교 테니스선수를 중심으로

이중숙,강재형,유창재,양정옥,이훈식 가톨릭대학교 자연과학연구소 2001 自然科學論文集 Vol.9 No.-

This study selected each seven high-school tennis players of male and female and analyzed the M.RA according to the type of tennis service and the Muscle Active Potential of M.ES by using an electromyogram system. The results as follows; 1.The Muscle Active Potential of female tennis players is higher than that of male tennis players in M.RA according to Tennis Service Types. 2.The Muscle Active Potential of female tennis players is higher than that of male players in M.ES according to Tennis Serrice Types. 3.In male and female M.RA, the Muscle Active Potential of right and left showed the contrary results. 4.In male and female M.ES, the Muscle Active Potential is high in the second service in the case of male and in the first service in the case of female. 5.Female tennis player can improve efficiency in the aspect of biomechanics by strengthening the muscle of M.RA and M.ES. 6.The second service of male tennis player showed less efficient Muscle Active Potential than his first service because of excessive trunk muscle twist.

산업보건관리자를 위한 소음성 난청 예방지침서의 개발

이원철,유경혜,장성실,이세훈,구정완,김현욱,노영만,이경재,김형아 가톨릭 대학 산업의학 쎈타 산업의학연구소 1996 韓國의 産業醫學 Vol.35 No.1

Occupational hearing loss became the leading occupational disease by constioution 56% of all work-related diseases in Korea. However, the prevalance rates showed wide variations among the specific medical examination providers and were very low(0.8-1.4%) compared with those 8.4% in Japan. The sources of these variations was regarded in the preview study as 1) Employee audometric estings were primarily conducted by nurses and nurse-aids who had no formal training for audiometry; 2) althought audiometric testing equipment used by the spedific medical examination providers, it should be periodically calibrated, a significant number of them were not properly calibrated : 3) Initial audiometric testings were conducted at any available spaces in workplace or at the testing room without considering bachground noise level. One of solutions that can be done for sloving surrent noise related problems is to provide a noise related manual for the occupational health managers including doctors, nurses and hyginests. This study was done to provide such a manual. The main core of the manual was hearing conservation program. And the contents of the manual was the same as the main frame of hearing conservation program such as noise survey, provided of the diagram showing the core contents of each factor of the hearing conservation program. Hopefully, this manual will be the references of the standardized performances of the specific medical examination providers.

특수건강진단의 건강관리구분과 그 개선방안에 관한 연구

이승한,이원철,김형아,장성실 大韓産業醫學會 1994 대한직업환경의학회지 Vol.6 No.2

The findings of special medical examinations are not infrequently summarized in the form of health classification. This procedure seems valuable for the protection of workers' health from personal health as well as public health point of view, because health classification of workers provides occupational health workers with birds eye view informations in terms of medical, placement and educational procedures, which are to be followed after medical examinations. In this study, the authors have reviews the existing systems of special medical examinations and health classifications in different countries and those recommended by international organizations for the purpose of improving the current system in Korea. The important issues covered in this review in relation to health classification of workers included the categories of diseases, the examination and laboratory tests, the criteria for health level classification and the health instructions to be given at each health levels. It was found that the current health classification system in Korea could be featured as follows: 1. Special medical examinations are always carried out in combination with general medical examinations, contributing to the detection of risk factors of occupational diseases. 2. The selection of examinees is always made on the basis of work environment monitoring, often resulting in the underdetection of exposed workers. 3. Even if the test items are properly selected according to the principles of occupational medicine, further review is required in the light of recent advances in the pathogenesis as well as early changes of occupational diseases and epidemiologic principles. 4. The criteria for the classification of health levels are to be reviewed on the basis of clinical eqidemiology. 5. The health instructions covered only medical and placement aspects, failing to introduce the concept of health promotion.

흰쥐 가슴샘을 지배하는 운동 및 감각신경세포체의 표지부위

이창현,장극선,김형태,이무삼 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.1

비글개에서 분만 후 자궁수복의 연속적 초음파상

손형원, 김홍석, 김형진, 김일화, 이기창, 이청산, 강현구 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

다단계 반응 및 단일 화염 표면모델을 이용한 석탄입자의 연소특성에 관한 수치적 연구

이창형,박원희,민동호,김태국 중앙대학교 기술과학연구소 2000 기술과학연구소 논문집 Vol.30 No.-

석탄 연소장치의 설계나 석탄 연소의 최적 조업조건 등을 추정하기 위하여 석찬 연소에 관한 많은 연구가 진행되어 왔지만 석탄 자체의 특성인 휘발분, char 그리고 회분 등의 분율과 성분에 따른 복잡성 때문에 일반적인 석탄의 연속특성을 규명하기란 쉬운 일이 아니다. 본 연구에서는 석탄의 수분 증발, 휘발, char 연소 등으로 진행되는 연소과정을 수치해석적으로 Modeling 함으로써 고체연료의 연소 특성을 파악하였다. 석찬의 종류, 크기, 주변기체의 온도, 유속, 방사율, 그리고 이산화탄소와 산소의 질량분율에 따른 연소특성을 파악한 결과 입자의 크기가 클수록, 주변기체의 유속이 빠를수록 연소가 빨리 진행되며, 주변기체의 온도와 방사율, 그리고 이산화탄소와 산소의 질량분율이 클수록 연소 진행이 빨라짐을 알 수 있다. 그러나 이산화탄소와 산소의 질량분율은 휘발분의 휘발에 거의 영향을 미치지 않음을 알 수 있다. 따라서 본 논문에서 고려한 인자들은 고체연료를 사용하는 연소시스템의 설계에 중요한 영향을 미치는 인자임을 알 수 있다. A systematic study on coal combustion is needed to design the coal combustion systems and to search for the best operating condition. In this study, we studied the coal particle combustion by considering the evaporation of moisture, two-reaction model of daf coal and heterogeneous carbon reaction of char which could be observed during the coal combustion. To study on the characteristics of the coal combustion, types and sizes of coal, temperatures and velocities of gas around the coal particle, emmisivities and concentrations of CO_2 and O_2 were considered for the parametric study. The rate of combustion is observed to be faster for smaller particle sizes and for faster gas velocities. As the temperature, emissivity and concentrations of CO_2 and O_2 of the environment become higher, the coal combustion rate becomes faster. However, the concentrations of CO_2 and O_2 didn't appreciably affect the devolatilization of the daf coal. From the result of this study, the parameters considered in this study can be important for the design of various coal combustion systems.