Expression of cellular retinoic acid-binding protein-I (CRABP-I) in the cerebrospinal fluid of adult onset moyamoya disease and its association with clinical presentation and postoperative haemodynamic change

Jeon, Jin Sue,Ahn, Jun Hyong,Moon, Youn-joo,Cho, Won-Sang,Son, Young-Je,Kim, Seung-Ki,Wang, Kyu-Chang,Bang, Jae Seung,Kang, Hyun-Seung,Kim, Jeong Eun,Oh, Chang Wan BMJ Publishing Group Ltd 2014 Journal of neurology, neurosurgery and psychiatry Vol.85 No.7

<P><B>Objective</B></P><P>The elevation of cellular retinoic acid-binding protein-I (CRABP-I) has been suggested as a candidate in the pathogenesis of paediatric moyamoya disease (MMD). However, few studies have addressed CRABP-I in adult onset MMD. The aim of this study was to examine the expression of CRABP-I in the cerebrospinal fluid (CSF) of adult onset MMD, and to evaluate its association with clinical presentation and postoperative haemodynamic change.</P><P><B>Methods</B></P><P>This study examined the CSF from 103 patients: bilateral MMD, n=58 (56.3%); unilateral MMD, n=19 (18.4%); atherosclerotic cerebrovascular disease (ACVD), n=21 (20.4%); and control group, n=5 (4.9%). The intensity of CRABP-I was confirmed by western blotting and expressed as the median (25th–75th percentile). The differences in CRABP-I expression according to disease entity (unilateral MMD vs bilateral MMD vs ACVD), initial presenting symptoms (haemorrhage vs ischaemia) and postoperative haemodynamic change (vascular reserve in single photon emission CT and basal collateral vessels in digital subtraction angiography) were analysed.</P><P><B>Results</B></P><P>CRABP-I intensities in bilateral MMD (1.45(0.86–2.52)) were significantly higher than in unilateral MMD (0.91(0.78–1.20)) (p=0.044) or ACVD (0.85(0.66–1.11)) (p=0.004). No significant differences were noted based on the initial presenting symptoms (p=0.687). CRABP-I was not associated with improvement in vascular reserve (p=0.327), but with decrease in basal collateral vessels (p=0.023) postoperatively.</P><P><B>Conclusions</B></P><P>Higher CRABP-I in the CSF can be associated with typical bilateral MMD pathogenesis in adults. Additionally, postoperative basal collateral change may be related to the degree of CRABP-I expression.</P>

6-OHDA가 흰쥐 등쪽솔기핵내 도파민성 신경세포와 별아교세포에 미치는 영향

홍은석(Eun Seog Hong),남성안(Seong Ahn Nam),조승묵(Seung Mook Jo),강태천(Tae-Cheon Kang),원무호(Moo Ho Won),최창도(Chang Do Choi) 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.4

신경독성물질인 6-hydroxydopamine (6-OHDA)이 중추신경계통 내 도파민성 신경세포에 미치는 영향과 이에 따른 별아교 세포들의 반응을 형태학적으로 규명하고자 본 연구를 시행하였다. 흰쥐의 가쪽뇌실에 6-OHDA를 투여한 후 면역조직화학 염색을 시행하여 광학현미경 하에서 등쪽솔기핵 내 도파민성 신경세포에 나타나는 퇴행성변화와 주위 별아교세포들의 반응양상 등을 경시적으로 제 3일, 제 5일, 제10일 및 제20일에서 관찰하였던 바 아래와 같은 결과를 얻었다. 6-OHDA에 의한 도파민성 신경세포의 퇴행성변화는 약물투여후 제 3일군에서 세포돌기가 팽대되고, 제 5일군과 제 10일군에서는 세포돌기의 소실과 함께 세포체가 위축되었고, 제20일군에서는 세포체가 소실되었다. 또한 6-OHDA에 의해 도파민성 신경세포들의 손상이 진행되면서 별아교세포들의 반응양상은 제 5일에서 대조군에 비해 급격하게 증가하였으며, 제20일에 이르러 최고값을 보였다. 이상의 결과로부터 6-OHDA가 중추신경계통 내 도파민성 신경세포들에 선택적이고 강한 세포독성을 유발하여 퇴행성 변화가 진행되고, 세포 손상이 시간 경과에 따라 진행되면서 주위의 별아교세포들이 지속적으로 증가된 활동을 보인다는 사실을 알게 되었다. This study was designed to clarify the cytotoxic effects of 6-hydroxydopamine (6-OHDA) on the dopaminergic neurons and astrocytes in the dorsal raphe nucleus (DRN), and to investigate neurodegenerative changes by immunohistochemistry. Adult male rats (Sprague-Dawley strain) weighing from 250 to 350 g were used as experimental animals. 6-OHDA (100 μg dissolved in 0.1% ascorbic acid) was injected into the lateral ventricle of the rat brain with the Hamilton syringe. The control rats were treated with the similar volume of 0.1 % ascorbic acid. The rats were sacrificed at the 3rd, 5th, 10th and 20th day, respectively, after the injection of 6-OHDA. The cytotoxicity of 6-OHDA resulted in severe neurodegeneration of the dopaminergic neurons in the DRN. In the 3rd day, the dopaminergic fibers were dilated. In the 5th and 10th days, the dopaminergic fibers were depleted, and dopaminergic cell bodies were shrunken. In the 20th day, the dopaminergic cell bodies were almost completely disappeared. Astroglial reactions induced by 6-OHDA were also observed in the DRN. In the 5th day, astrocytes were significantly increased as compared with that of the control value. The value were reached at its maximum by the 20th day. Based on the present results, it suggests that 6-OHDA may act as a specific neurotoxin to dopaminergic neurons in the DRN, and induce severe neurodegenerative changes. Also, it suggests that the astroglial reaction in the DRN is gradually activated during the neurodegerative changes

GaAs MESFET의 채널전하에 의한 전위장벽 강하특성

원창섭,안형근,한득영 건국대학교 산업기술연구원 2003 건국기술연구논문지 Vol.28 No.-

The gate leakage current is first calculated using the experimental method between gate and drain by opening source electrode. Next, the gate to drain current has been obtained with a ground source. The difference of two current has been tested and provide that the electric field that generated by channel charge effect to Schottky barrier height against the image force lowering effect.

뇌성마비아의 모발내 중금속 함량 분석

안경희,김희상,유창현,윤동환,홍장혁,유승돈,이준원 경희대학교 2001 慶熙醫學 Vol.17 No.1

삼출성 중이염 환자에서의 혈중 및 아데노이드 면역글로불린 E의 면역혈청학적 연구

장혁순,강성호,유창수,김성현,이병돈,박영진,윤세동,안희영,강주원 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Otitis media with effusion (OME) is one of the most common disease in children but its pathogenesis is not clear. In particular the role of allergy has revealed conflicting support with widely varing opinions as to its importance. Within recent years, striking biochemical and immunochemical analytic techniques have opened a new approach the study of otitis media with effusion. So we focused on distributions of IgE in adenoid tissue and serum IgE levels. Authors compared the serum IgE and the distribution of IgE in adenoid tissue between the 20 patients underwent tonsillectomy, adenoidectomy and ventilating tube insertion and control group of 11 patients underwent tonsillectomy and adenoidectomy. The results were as follows; 1. In the distribution of IgE in adenoid tissue, there was a significant increase in the group of patients with OME compared to the control group (P<0.01). 2. In the analysis of serum IgE levels, the levels of IgE in the OME patients an control group were higher than normal level, although the level of IgE in the OME patients were less than that of control group, there was no significance P>0.05).

Balb/c와 DDY계 생쥐 오금림프절의 구조에 관한 형태학적 연구

이창호,이원규,허영범,김정혜,한희경 慶熙大學校 1993 論文集 Vol.22 No.-

This study was aimed to clarify the distribution of lymph follicles, germinal centers and deep cortex structure in the lymph nodes of the Balb/c and DDY strain mice. Male adult mice were adopted in this study. After mice were sacrifed, the fight popliteal lymph nodes were removed in the popliteal fossa and fixed in Zenker-formol solution for 3 hours and embedded in plastic resin. The cutting thickness was 3㎛ serially and one of every two section was stained in May-Gru¨nwald and Giemsa solution. The results obtained from this study were as follows; 1. The size of lymph node was 0.750 mm in Balb/c mice and 0.968mm in DDY mice. 2. The average number of lymph follicles per popliteal lymph node was 15.5 in Balb/c mice, but 16.0 in DDY mice. The major of the lymph follicles situated in peripheral cortex overlying the uint(PCOU) in Balb/c. 3. The most transverse diameters of each strain lymph follicles was between 0.01 to 0.19mm. 4. The characteristic difference was number of germinal centers, DDY mice lymph follicles contained germinal centers in 13.25/lymph node, but Balb/c mice lymph follicles contained it in 5.75/node. 5. The morphology of deep cortex was not significant between each strain.

공 컨테이너를 고려한 컨테이너 내륙수송에서의 차량배치문제

윤원영,안창근,최용석 한국경영과학회 1999 한국경영과학회지 Vol.24 No.4

In the container transportation of inland depots the empty container is an important issue. The truck dispatching problem in this paper is considered under restriction about empty containers. When a full container arrives in a depot by truck, after unloading operation the full container is changed to an empty container. After loading operation an empty container is changed to a full container. We suggest an integrated approach to the problem of simultaneously managing empty truck and container movements. We would like to find the truck dispatching method minimizing empty truck movements and empty container movements. In this paper, a mixed integer programming model is developed to obtain the optimal truck dispatching in container transportation and heuristic is developed to give a good solution in much shorter time. The numerical examples are studied and the proposed heuristic is also compared with the result of numerical examples.

인위적 근피로 유발후 단축성과 신장성 근수축 운동시 근 토크의 변화

강영석,안창영,윤성원,나윤수 서울여자대학교 자연과학연구소 1995 자연과학연구논문집 Vol.6 No.-

The purpose of this study was to investigate the change of the peak torque, average power and total work of muscle function in the fatigue during eccentric(ECC) and concentric contraction (Con). Subjects were seven (7) adults man, who were paticipated in the ECC and CON experiments. Their gastronemus muscle were measured by CYBEX(Lurnax Co.) 6000system. Muscle fatigue was induced by 50 repetitionplantar flexion at 180 /sec load speed. In pre and post test, peak, average power and total work were analized when subjects were tested plantar flexion at 180/sec load speed. the summary of this results is as following : 1. Peak torque in the ECC were significantly higher than that of the CON. 2. Average power and total work of the Eccentric contraction appeared a higher than that of the Concentric contraction significantly. As a results. peak torque, average power and total workin the ECC was significantly higher than that of the CON throughout the experiments. In this fact, Mechanical efficiency in the ECC will expect to be higher than that of the CON.

지방 국립대학의 우수학생 유치방안에 관한 연구 : 부산대학교를 중심으로

이원호,안창규,정영홍,황해익,주철안 釜山大學校 敎育硏究所 1992 敎育硏究 Vol.2 No.1

Korean universities have developed rapidly since its liberation from hapanese rule in 1945. Korean higher education system has contributed to the socio-economic development of the country. However, Korean higher educational system has some problems. One of the problems is its unbalanced development of higher educational institutions between the capital city and other cities. Koreans traditionally regarded highly colleges and universities in the capital city. The government including private foundations have invested large resources in building colleges and universities in cities and providences had been neglected. However, colleges and universities play crucial role in the local, regional development of the country. It is important to raise academic quality of the colleges and universities in non-capital cities. The quality of colleges and universities depended primarily on faculty, finance, etc. Therefore, it is an imperative condition to recruit academically excellent students for the development of colleges and universities in non-capital cities. Three research questions have guided the research. 1) How the college entrants' academic performance has changed. 2) What colleges and universities have utilized admission policy to recruit excellent students. The research has found out the following results. 1) The academic performances of college entrants in non-capital city were lowered in the 1980s. 2) Colleges and universities have emphasized the academic excellence, which is primarily related to research and academic activities of the faculty. 3) High school students and teachers in Pusan city give local university lower rate than what it is The findings suggest that local college and university make sustained, ststematic efforts in order to develop its status among the institutions. The efforts should include short-term and long-term policies : 1) Marketing activities about scholarship programs, graduates' career prospects, facilities, and the faculty etc. 2) Hiring excellent faculty members 3) Strengthening graduates' gaining job 4) furnishing required facilities and making attractive campus 5) Upgrading the quality of academic and research activities

만성 췌장염에서 Foipan^R 투여시 효과와 안정성

이창수,강원택,이충섭,안득수 의과학연구소 1990 全北醫大論文集 Vol.14 No.4

To evaluate the clinical effect and safety of long-term administration of Camostat Mesilat (FOIPAN^R) in chronic pancreatitis, we had given Foipan 200㎎ 샹 orally to twenty patients with chronic pancreatitis for 4 weeks. Foipan improved the subjective symptoms and objective signs of patients with chronic panceatitis without signficant hematologic and biochemical changes. There were no serious side effects excent only mild thirst and Constipation in two cases. In conclusion, Fiopan^R seems to bo a fairly safe and effective drug for relief of accompanying symptoms in chronic pancreatitis.