과채류 접목재배 및 접목묘 생산현황

장윤아,여경환,이준구,최장선,엄영철,이상규,윤무경 한국종자연구회 2013 종자과학과 산업 Vol.10 No.2

종묘관련 분쟁사례 및 관리방안

장윤아,이지원,최근진,안세웅,박동금 한국종자연구회 2017 종자과학과 산업 Vol.13 No.1

Light Intensity and Lettuce Growth in an Apartment Balcony Affected by the Direction of Balcony

장윤아,정순진,한경숙,이상미,서정남,김광진 인간식물환경학회 2017 인간식물환경학회지 Vol.20 No.5

Light is very important for plant growth and a major limiting factor in indoor plant cultivation, such as in balconies. This study was conducted to investigate the light intensity and lettuce (Latuca sativa L.) growth in an apartment balcony affected by the direction of balcony. Photosynthetic photon flux (PPF), air temperature, and relative humidity in the east-, south-, or west-facing balcony were measured for a year. The lettuce was grown in the east-, south-, or west-facing balcony. The time and amount of light entering into the balcony differed according to the direction of the balcony window. Whereas light entered into the east-facing balcony from sunrise to noon, it entered into the west-facing balcony afternoon before sunset. The temperature and humidity in the balcony also varied according to the direction of the balcony window. Daily Light Integral (DLI) in the south-facing balcony was 20~50% of outdoor and DLI in east- or west-facing balconies was 10~20%. Also, the environmental characteristics of the balconies were affected seasonally. In the spring (from March to May), the DLI was around 10 mol・m-2 ・day-1 and the average temperature was around 25°C. But the DLI was below 10 mol・m-2 ・day-1 and the average temperature and relative humidity were around 30°C and over 60%, respectively, in the summer (from June to August). In the balcony, the average temperature was maintained around 10°C even in winter, which is a condition of freezing outdoor. Growth of the lettuce in the south-facing balcony was better than that of the other directions. This is attributed to better environmental conditions of south-facing balcony. Therefore, considering the environmental characteristics of the balcony, selection and management of crops with relatively low light demand like leafy vegetables are required. It is also recommended to cultivate crops from autumn to spring.

종이포트 묘 육묘시 양분관리, 육묘일수 및 정식 후 야온에 따른 오이의 생육

장윤아,안세웅,전희,이희주,위승환 (사) 한국생물환경조절학회 2019 생물환경조절학회지 Vol.28 No.4

This study was conducted to investigate the growth of grafted cucumber seedlings in biodegradable paper pot trays influenced by seedling age, nutrient management before transplanting, and night temperature after transplanting. Grafted cucumber seedlings in paper pot trays were supplied with different nutrient solution concentrations of 0.5 x full strength (S) (EC 0.8 dS·m-1), 1.0S(EC 1.6 dS·m-1), 2.0S(EC 3.2 dS·m-1) two times a week until transplanting. 26, 33, 40, and 47 day-old cucumber grafted seedlings were transplanted and grown at three levels of night temperature (10, 15, and 25°C) during ten days. Increasing nutrient solution concentration enhanced the shoot length, number of leaves, leaf area, dry weight, and relative growth rate of seedlings. With increasing seedling age, the differences in growth were greater among nutrient treatments. The dry matter percentage increased with the seedling age, but was lower with higher nutrient concentration. The specific leaf area showed the opposite results. In cucumbers transplanted at 26- or 33-day seedling ages, night temperature did not affect the growth at ten days after transplanting. However, the growth of 40 or 47 day-old seedling decreased at 10°C. Compared with 25°C, the dry weight of cucumbers transplanted at 40- or 47-day seedling ages was depressed by 58% or 71%, respectively, at 10°C. Accordingly, it was concluded that the optimum nutrient solution concentrations and seedling age for the production of grafted cucumber seedlings in biodegradable paper pot trays can be 1.0S and about 30 days, respectively, and night temperature should be maintained at the range of 15-25°C for promoting the growth after transplanting. 본 논문에서는 생분해성 종이포트를 이용한 오이 접목 묘 육묘시 추비용 양액의 농도와 육묘일수, 정식 후 야 간 온도에 따른 오이의 생육을 검토하였다. 오이 종이포 트묘 접목활착 종료 후 육묘 중 시비 농도를 0.5S(EC 0.8dS·m-1), 1.0S(EC 1.6dS·m-1), 2.0S(EC 3.2dS·m-1)의 3 수준으로 처리한 뒤, 육묘일수를 파종 후 26, 33, 40, 47일로 달리하여 정식하였다. 정식 직후 야간 온도를 10, 15, 25°C 3수준으로 조합, 처리하여 10일 동안 재배 하였다. 육묘기간 중 오이 종이포트묘의 초장, 엽수, 엽 면적, 건물중, 및 상대생장률은 추비용 양액의 농도가 높아질수록 증가하였으며, 육묘일수가 경과할수록 처리 에 따른 차이는 더 커졌다. 건물률은 육묘일수가 경과함 에 따라 증가하는 경향을 보였으나, 양액의 농도가 높을 수록 낮았다. 반면 비엽면적은 육묘일수가 길어질수록 감소하였고, 양액 농도가 높을수록 높은 값을 나타냈다. 정식 10일 후 오이의 생육은 육묘일수가 증가할수록 초 장, 엽면적, 건물중에 있어서 높은 값을 나타냈으나, 상 대생장률은 감소하였다. 육묘일수 26일, 30일의 경우 정 식 후 오이의 생육은 야간 온도의 영향이 크지 않았으 나, 육묘일수가 길어져 40일이상 육묘한 묘는 정식 후 10°C 정도의 저온에서 활착이 지연되어 생육이 저조하 였다. 따라서 오이 종이포트 접목묘 생산시 추비용 양액 농도 1S, 육묘일수는 30일 내외가 추천되며, 정식 후 활 착 및 생육 촉진을 위해 15-25℃ 범위의 야간온도 관리가 요구된다.

소형과 수박의 생육과 과실 품질 증진을 위한 야생종 수박 대목 이용

장윤아,문지혜,안세웅,김상규,허윤찬,이희주,위승환,전희 (사) 한국생물환경조절학회 2019 생물환경조절학회지 Vol.28 No.4

최근 식품 소비패턴 변화로 과실 무게 2~3kg 내외의 소형과 수박에 대한 관심과 이용이 증가하는 추세이다. 호박 대목을 이용한 수박 접목재배는 덩굴쪼김병 등 토 양 전염성 병 관리에 유용하나, 왕성한 생육으로 과실 품질이 저하하는 문제가 제기되고 있다. 본 연구에서는 덩굴쪼김병 저항성 수박 공대를 이용한 접목재배시 소형 과 수박의 생육 및 착과 특성, 과실의 품질을 검토하였 다. 덩굴쪼김병에 저항성이 있는 것으로 평가된 야생종 수박 5종(Galactica, IT 208441, PI 482322, PI 500303, PI 593358)을 대목으로 이용하여, 편엽합접으로 접목한 뒤 비닐하우스에서 재배, 수확하여 생육과 과실 품질을 조사하였다. 접수는 소과형 전용 수박품종 ‘미니스타’를 이용하였으며, 시판 대목 참박 ‘불로장생’과 호박 ‘신토 좌’을 대조로 비교하였다. 수박 대목 ‘PI 593358’에 접 목한 처리구가 ‘신토좌’ 대목에 접목한 처리구와 함께 우수한 뿌리 발달 등 왕성한 생육을 보이며 빠른 착과 가 이루어졌으나, 과실 품질에 있어 ‘total soluble solids(TSS)’ 값과 관능평가 값이 낮았다. 반면 수박 대 목 ‘PI 482322’에 접목한 수박의 관능평가 값은 높은 편이었다. 따라서 소형과 수박 접목재배시 야생종 수박 ‘PI 482322’ 등을 대목으로 이용함으로써 안정적인 생육 및 과실 품질 확보가 가능함을 확인할 수 있었다. 검토 된 야생종 수박은 호박 및 박 대목을 대체하는 대목 개 발에 활용될 수 있을 것으로 기대된다. The interest in mini watermelon (Citrullus lanatus) with small fruits weighing 2-3 kg has increased by the increasing trend in one-person households and consequent tendency to consume small meals. Watermelon grafting onto cucurbit rootstocks is a very effective way to control soil-borne diseases, such as Fusarium wilt; however, this practice negatively impacts the fruit quality. This study was conducted to investigate the growth, fruit set, and fruit quality of mini watermelon grafted onto wild watermelon accessions (Citrullus spp.) resistant to Fusarium wilt. Five watermelon accessions (Galactica, IT 208441, PI 482322, PI 500303, and PI 593358) were evaluated as rootstocks for the mini watermelon “Ministar”. Non-grafted “Ministar” and “Ministar” grafted onto “Shintozwa” (Cucurbita maxima D. C. moschata D.) or “Bullojangsaeng” (Lagenaria leucantha) were used as controls. The roots of the transplants grafted onto “PI 593358” and “Shintozwa” weighed more than those on other rootstocks. Additionally, the transplants on “PI 593358” showed better growth and fruit set in the field than the other transplants. However, the total soluble solid contents and fruit quality indices of the transplants on “PI 593358” and “Shintozwa” were lower, whereas the total fruit quality index of those on “PI 482322” was higher. Thus, the wild watermelon accessions tested can potentially be used as basic germplasm for developing watermelon rootstocks instead of cucurbit rootstocks. The most promising accession for this purpose was found to be “PI 482322”.

日韓語のナラティブ構築過程に見られる 聞き手の評価と寄与

장윤아 한국일본어교육학회 2022 日本語敎育 Vol.- No.100

본고에서는 한일어 대화에서 화자가 내러티브를 구축해가는 도중에 청자가 화자의 내러티브의 적절성과 가치를 어떻게 평가하고 있으며 청자의 평가 발화가 내러티브 구축에 어떠한 영향을 미치고 있는지를 상호작용의 관점에서 비교분석하였다. 그 결과, 한일양국의 대화에서 <동일 시점제시> 발화의 사용비율이 유사하게 나타났다. 하지만, 일본어 대화에 비교하여 한국어 대화에서는 <감상표명> 발화와 <다른 시점제시> 발화의 사용비율이 높게 나타났으며, 일본어 대화에서는 <감정표출> 발화의 사용비율이 높게 나타났다. <다른 시점제시>의 경우, 사용양상에 한일차이가 관찰되는데, 한국어 대화에서는 내러티브의 의미와 가치를 교섭하는 수단으로 사용되지만, 일본어 대화에서는 웃음을 자아내는 농담으로 사용되어 내러티브의 가치를 평가하는 수단으로 사용되는 경향이 있다. 또한, 청자의 평가 발화가 화자의 어떠한 유형의 발화 후에 사용되는지에도 한일간에 상이점이 관찰되었다. 향후에는 화자의 내러티브의 내용적 전개에 기여하는 청자의 협동적인 언어행동에 주목하여 분석을 지속해 나아갈 필요가 있다.

日韓両国の同性間の日常会話における 「同意・共感」の相互行為 -ジェンダー差を中心に-

장윤아 한국일어일문학회 2018 日語日文學硏究 Vol.107 No.1

The purpose of this study is to analyze utterances which express‘Agreement /Empathy’ in Korean and Japanese same-sex conversation between friends. I have found the following similarities and gender differences. (A) Comparing the frequency of usage: Both Japanese and Korean women tend to use more ‘Agreement/Empathy’ utterances then men do. (B) Comparing the proportions of usage: In Japanese and Korean men’s conversation, the proportions of single speaker expressing ‘Agreement/ Empathy’ were higher then women’s conversation. In the other hand, in Japanese and Korean women’s conversations, the proportions of both speakers producing ‘Agreement/Empathy’ were higher then men’s conversation. (C) Comparing the interactions: Both Japanese and Korean men tend to express ‘Agreement/Empathy’ only once and addressees who affirmed the common ground tend to developed conversation without showing any response. (D) Comparing the interactions: Both Japanese and Korean women tend to repeat their ‘Agreement/Empathy’ utterances and women tend to developed conversation by adding ‘Agreement/Empathy’ utterances to what previous speaker has said. The gender differences in frequency of usage and interaction patterns of ‘Agreement/Empathy’ show that there may be different norms and different way of usage for men and women. 본 연구에서는 한일 양국 동성친구간의 일상적인 대화에서 「동의・공감」의 발화가 어떻게 사용되고 있는지를 상호행위 레벨에서 분석하고 그 사용양상을 젠더차의 관점에서 살펴보았다. 그 결과는 다음과 같다. (A)「동의・공감」발화의 사용빈도는 한일 모두 남성간의 대화에 비해 여성간의 대화에서 사용빈도가 높았다. (B) 단독적인「동의・공감」의 사용비율은 남성간의 대화가 높았고, 상호적인「동의・공감」의 사용비율은 여성간의 대화에서 보다 높게 나타났다. (C) 남성간의 대화에서 「동의・공감」은 한 명의 화자에 의해 단발적으로 발화되는 경향이 있고,「동의・공감」발화의 수신자는 상대와의 공통기반을 확인한 후, 그대로 대화를 전개시키는 경향이 강했다. (D) 여성간의 대화에서 「동의・공감」은 한 명의 화자에 의해 연사적으로 발화되는 경향이 있으며, 두 명의 대화 참가자가 「동의・공감」의 발화를 서로 쌓아가며 대화를 전개시키는 상호행위가 이루어지는 경향이 있다. 「동의・공감」의 상대사용빈도와 상호행위 패턴에 나타나는 젠더차이는 상호행위 속에서 남녀가 「동의・공감」의 발화를 어떠한 수단으로 사용하고, 「동의・공감」발화를 어떻게 해석하고 있는지에 대한 차이와 깊게 관련된다고 볼 수 있다.

Functional Defects in Type 3 Innate Lymphoid Cells and Classical Monocytes in a Patient with Hyper-IgE Syndrome

장윤아,강성윤,김지현,강혜련,김혜영 대한면역학회 2017 Immune Network Vol.17 No.5

Hyper-IgE syndrome (HIES) is a very rare primary immune deficiency characterized by elevated serum IgE levels, recurrent bacterial infections, chronic dermatitis, and connective tissue abnormalities. Autosomal dominant (AD) HIES involves a mutation in signal transducer and activator of transcription 3 (STAT3) that leads to an impaired TH17 response. STAT3 signaling is also involved in the function of RORγt+ type 3 innate lymphoid cells (ILC3s) and RORγt+TH17 cells. The aim of this study was to investigate the role of innate immune cells such as innate lymphoid cells (ILCs), granulocytes, and monocytes in a patient with HIES. Peripheral blood mononuclear cells (PBMCs) from a patient with HIES and three age-matched healthy controls were obtained for the analysis of the innate and adaptive immune cells. The frequencies of ILCs in PBMCs were lower in the patient with HIES than in the controls. Moreover, granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-17A produced by ILC3s in PBMCs were lower in the patient with HIES than the controls. Compared with the controls, classical monocytes (CD14+CD16low), which have a high antimicrobial capability, were also lower in the patient with HIES, while non-classical monocytes (CD14lowCD16+) as well as intermediate monocytes (CD14+CD16intermediate) were higher. Taken together, these results indicate that the impaired immune defense against pathogenic microbes in the patient with HIES might be partially explained by functional defects in ILC3s and inflammatory monocytes.

Effects of Photosynthetic Photon Flux and Carbon Dioxide Concentration on the Photosynthesis and Growth of Grafted Pepper Transplants during Healing and Acclimatization

장윤아,문보흠,도경란,엄영철,전창후 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.5

In the production of grafted transplants, healing and acclimatization are the most critical processes forsurvival. We investigated the influence of the photosynthetic photon flux (PPF) and the carbon dioxide (CO2)concentration during healing and acclimatization on the photosynthetic characteristics and growth of grafted peppertransplants to determine the optimum environmental conditions for healing and acclimatization in a healing chamberwith artificial lighting source. Grafted pepper transplants were healed and acclimatized under two levels of CO2 (374or 1,013 μmol·mol-1) and four levels of PPF (dark, 50, 98 or 147 μmol·m-2·s-1) for six days. The CO2 exchange ratesof the grafted pepper transplants significantly increased with increasing PPF during healing and acclimatization. TheCO2 exchange rates were higher under elevated CO2 concentrations than ambient CO2 concentration. The effect ofCO2 enrichment was greater in low light intensity. The CO2 exchange rates at 50, 98 or 147 μmol·m-2·s-1 under elevatedCO2 concentrations were 511, 261, and 172%, respectively, compared to the ambient CO2 concentrations. The increaseof photosynthesis led to an improvement in growth. The SPAD value, dry weight and leaf area were greater underhigher PPF and CO2 concentrations. PPF also influenced the anatomical structures of the leaves, and the palisade andspongy tissue cells of the leaves irradiated with higher PPF were aligned more densely, with more chloroplasts andsmall empty space. When compared to the tunnel in the greenhouse with natural light, healing and acclimatizationunder high CO2 (1,000 μmol·mol-1) and PPF (150 μmol·m-2·s-1) conditions in the healing chamber promoted the growthand graft union formation of grafted pepper transplants. The results suggested that high-quality grafted pepper seedlingscould be achieved by healing and acclimatization in a healing chamber where optimal conditions such as high PPFand CO2 were maintained within the range evaluated in this experiment.

Effect of Grafting on Growth and Incidence of Phytophthora Blight and Bacterial Wilt of Pepper (Capsicum annuum L.)

장윤아,양은영,조명철,엄영철,고관달,전창후 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.1

This study was conducted to investigate the effect of grafting using commercial rootstocks and breeding lines on growth and resistance to both Phytophthora blight (Phytophthora capsici) and bacterial wilt (Ralstonia solanacearum),and to evaluate the breeding lines as candidates rootstocks for grafted peppers. Capsicum annuum ‘Nokkwang’ (scion)was grafted onto five commercial rootstocks (‘Kataguruma’, ‘Konesianhot’, ‘Koregon PR-380’, ‘PR-power’, and ‘Tantan’)and nine breeding lines (‘PR 901’, ‘PR 919’, ‘PR 920’, ‘PR 921’, ‘PR 922’, ‘PR 927’, ‘PR 928’, ‘PR 929’, and ‘PR 930’). Graft-take percentage was over 80% in all the grafted seedlings except those grafted onto ‘PR 901’ (75%). The number of leaves, stem diameter, leaf area, and dry weight of shoot of grafted seedlings were greater than those of non-grafted seedlings. Mineral contents in the shoots of grafted seedlings were significantly different, depending on rootstock genotypes. Marketable yield ranged from 1.35 to 1.96 kg per plant depending on rootstock genotypes, and was highest in peppers grafted onto ‘PR 901’ (1.95 kg) and non-grafted peppers (1.96 kg), and lowest in those grafted onto ‘PR 928’ (1.35 kg). Gross yield was highest in peppers grafted onto ‘Konesianhot’ (2.64 kg). Among nine breeding lines, three rootstocks (‘PR 920’, ‘PR 921’, and ‘PR 922’) were selected as candidate rootstocks for the grafted pepper based on graft-take, growth, yield, fruit quality, and resistance to diseases. Three major pepper cultivars (‘Nokkwang’,‘Saengsaeng Matkkwari’, and ‘Shinhong’) were grafted onto those three breeding lines and ‘Tantan’ (control). Peppers grafted onto breeding lines of ‘PR 920’, ‘PR 921’, and ‘PR 922’ showed greater resistance to both Phytophthora blight and bacterial wilt without the decrease in yield and fruit quality. Accordingly, they were considered to be used as rootstocks resistant to both Phytophthora blight and bacterial wilt for pepper production.