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친환경 과원의 단감 ‘부유’(Diospyros kaki ‘Fuyu’) 과실의 동상해에 미치는 나무의 식재위치와 착과방향의 영향
강성구,안광환,최성태,도경란,조광식 한국유기농업학회 2014 韓國有機農業學會誌 Vol.22 No.4
본 연구는 친환경단감과원의 부유(Diospyros kaki ‘Fuyu’) 과실을 대상으로 창원과 창녕지역에서 발생한 저온내습(2012년 11월 2일)으로 인한 가을 동상해발생과 식재위치(표고) 및 나무 내에서의 착과 방향의 관계를 알아보고자 수행되었다. 창녕지역 과원의 과중과 과실횡경은 착과방향에 의해 강하게 영향을 받았으며(p<0.01), 과실종경과 동상해발생은 식재위치(p<0.01)와 깊은 관계가 있는 것으로 나타났다. 과실경도와 과실종경은 각각 착과방향과 식재위치의 영향을 받는 것으로 밝혀졌다(p<0.05). 특히 동상해는 과원 하단에 식재된 나무의 과실(15.2%)과 잎(약 90%)에서 심하게 나타나 동상해의 주요 요인이 과원의 지형적 특징인 것으로 나타났다. 과실 동상해를 입은 과실은 과피에 수침상 반점이나 흔적이 발생했다. 한편 창원지역의 과원에서는 과실횡경이 식재위치의 영향을 받은 것(p<0.05)을 제외하고 식재위치와 착과방향이 과실의 생육 및 동상해발생에 영향을 주지 않았다. We have analyzed the effect of planting site (elevation) and direction of fruiting on the frost damage (tolerance to transparent skin and blemishes) of persimmon (Diospyros kaki Thunb) ‘Fuyu’ fruit subjected to the early fall frost (November 2, 2012) in environment-friendly orchards of Changnyeong and Changwon (Gyeongsangnam-do Korea). In Changnyeong, the direction of fruiting showed a significant effect on the fruit weight and the fruit width (p<0.01), and the fruit firmness (p<0.05) while the planting site (elevation) did a highly significant effect on the fruit width and the frost damage (p<0.01), and fruit length (p<0.05). In particular, severe frost damage of fruit (15.2%) and leaves (about 90%) was observed in the plants located in the low elevation area of orchard. The damaged fruit had an transparent skin color and/or blemishes. In Changwon, there was no effect of the planting site and the direction of fruiting on the frost damage, and the fruit characteristics except the fruit width having been related to the planting site (p<0.05). So, the main effects for the fruit frost damage was a terrain factor around the orchards.
장인배,도경란,서수정,Jin Yu,장인복,문지원,Changhoo Chun 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.4
Ginseng, a semi-shade perennial plant, is greatly aff ected by light. However, light quality has only been studied in a fewpapers involving growth tests under monochromatic light or based on hairy root cultures. A plant factory with light-emittingdiodes (LEDs) may provide high precision and standardization of ginseng seedlings for transplanting, and plant responsesto light quality should be investigated for designing the optimal lighting conditions for this environment. In this study, variousratios of red light (R):blue light (B) were set from 100:0 to 0:100% at the same photosynthetic photon fl ux density of75 μmol m −2 s −1 . As R increased, the shoot length became longer from R75B25, resulting in the single treatment of R being1.68 times that of B. Compared to monochromatic R or B treatments, the overall growth of ginseng seedlings in R50B50treatment increased. In the mesophyll structure, mixed light as R50B50 or while LEDs increased starch grains, and onlyR treatment led to dense chloroplasts in palisade and spongy parenchyma cells. Increasing R ratios had negative eff ects onCO 2 assimilation rate ( A N ), light-saturated net photosynthesis rate ( A sat ), and chlorophyll parameters. The higher the R ratio,the higher the ginsenoside content in leaves, while roots were less aff ected by spectral changes. Monochromatic R inducedmalformation and senescence of ginseng leaves, while the addition of 25% B was suffi cient to prevent the abnormal developmentof leaves and dysfunctional photosynthetic operation of ginseng seedlings. The results suggest that combinations ofR and B should be considered when designing artifi cial lighting systems for a closed-type plant factory since R aff ects themorphological characteristics and ginsenoside content of ginseng seedlings.
황금배(Pyrus pyrifolia Nakai) 잎 조직으로부터 기내 신초 재분화
천재안,도경란,김세희,조강희,김현란,황해성 한국식물생명공학회 2012 식물생명공학회지 Vol.39 No.4
본 연구는 ‘황금배’(‘Whangkeumbae’)의 엽 절편체로부터형질전환 체계 확립을 위한 효율적인 재분화 체계 확립을 위해 식물 생장조절제와 주요 탄소원인 sucrose,sorbitol이 식물체 재분화에 미치는 영향을 조사하기 위해수행되었다. 잎 절편체를 MS 배지에 TDZ 농도를 각각0.1, 0.25, 0.5, 1, 2.5, 5 mg/L으로 하여 IBA 0.3 mg/L을 혼용처리한 결과 TDZ 0.25 mg/L 농도에서 61.1%의 가장 양호한 재분화율을 보였으며 TDZ 2.5 mg/L 이상의 농도에서는 신초의 재분화율이 급격히 감소하였다. IBA와 IAA 농도에 의한 신초의 재분화율은 TDZ 0.5 mg/L와 IAA 혼용 처리한 구에서 전체적으로 높은 신초의 재분화율을보였으며 특히 TDZ 0.5 mg/L + IAA 0.3 mg/L 처리구에서76.7%의 가장 높은 재분화율을 보였다. 서로 다른 탄소원으로써 sucrose와 sorbitol의 영향에 대하여 15 g/L, 30 g/L 농도로 처리한 결과 sorbitol 30 mg/L에서 가장 높은 재분화율을 보였고 또한 절편체 당 신초의 수도 3.5개로 가장양호하였다. 따라서 sorbitol이 sucrose보다 `황금배`의 신초의 재분화에 있어서 효과적이었다. In order to establish an efficient adventitious shoot regeneration conditions from leaf explants for Asian pear ‘Whangkeumbae’, the effect of concentration and kinds of plant growth regulator and carbon source was investigated. Leaf explants of cultures grown on Murashige and Skoog (MS) medium containing 8 g/L plant agar were used. When the medium contained 0.25 mg/L thidiazuron (TDZ) and 0.3 mg/L indolebutyric acid (IBA), the adventitious shoot regeneration rate (ASRR) was greater as 61.1% than others treated and higher TDZ concentrations (2.5 and 5 mg/L) treatment significantly reduced the ASRR. As the effect of IBA and indoleacetic acid (IAA)concentration on the ASRR, 0.5 mg/L TDZ plus different concentration of IAA exhibited relatively high ASRR and 0.5 mg/L TDZ plus 0.3 mg/L IAA showed the highest ASRR of 76.7%. Also the effect of sucrose and sorbitol as carbon source on regeneration was examined. The highest ASRR and the most shoots per explants averaged 94.4% and 3.49 by treatment of 30 mg/L sorbitol, respectably. Sorbitol is considered better carbon source than sucrose for shoot regeneration of ‘Whangkeumbae’ pear.
장기간 계대배양 된 장미 배발생 캘러스로부터 식물체 재분화 및 비정형체로부터 새로운 배발생캘러스 재생
이수영,도경란,천경성,김원희,권오현,이혜진 한국식물생명공학회 2014 식물생명공학회지 Vol.41 No.2
Long-term subcultured rose embryogenic calluses,which had been maintained for more than 5 to 6 years sincethe first embryogenesis from calluses induced from in vitroroots of rose, were identified as potential material for thedevelopment of transgenic plants. The first embryogeniccalluses from ‘Sweet Yellow’ and two breeding lines (KR056002and KR056006) were obtained in 2007 and 2009, respectively. Subsequently, we found that plants regenerated from longtermembryogenic calluses (LEC). Whereas the LEC from‘Sweet Yellow’ takes 3 to 4 months to regenerate plants,those of the two breeding lines take 4 to 5 months. Thisperiod of time is the same as that taken for plants to regeneratefrom the first embryogenic callus. New embryogenesis wasobserved from atypical bodies (ABs) that appeared duringthe process of long-term subculture. We found that it ispossible to use the AB as a material for new embryogenesis.
장인배,도경란,Hyunseung Hwang,서수정,Jin Yu,장인복,문지원,Changhoo Chun 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.4
To determine the optimal light intensity and enable plants to cope with various environmental stresses in plant factories, the morphological and photosynthetic characteristics of ginseng seedlings, including the secondary metabolites, were investigated under six light intensities: 25, 50, 75, 100, 125, and 150 μmol m−2 s−1. The shoot length increased with lower light intensity up to 75 μmol m−2 s−1, and the leaf area and specific leaf weight were greatest at 100 and 75 μmol m−2 s−1, respectively. Stomata frequency was higher from 100 μmol m−2 s−1 with higher light intensity, and single stomatal pore length also increased at 150 μmol m−2 s−1. Net photosynthetic rate at light saturation and net photosynthetic rate in growth condition increased sequentially up to 100 μmol m−2 s−1 before rapidly decreasing at 150 μmol m−2 s−1. The dark respiration rate and light compensation point were significantly high at 150 μmol m−2 s−1 only. Fv/Fm and chlorophyll content statistically differed from 63 and 84 days after seedling stand, respectively. Thus, the leaves withered to death with higher light intensity. The ginsenoside content in the roots significantly increased according to the light intensity, and the panaxadiol/panaxatriol (PT) ratio tended to decrease as the PT-type ginsenosides increased further. Taken together, the range of 75–100 μmol m−2 s−1 was found to be beneficial for growth, photosynthetic acclimation response, and total accumulated ginsenosides of ginseng seedlings.