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돼지 β-casein을 이용한 EGFP 발현 Knock-in 벡터의 구축 및 발현 검증
이상미,김혜민,문승주,강만종 사단법인 한국동물생명공학회 2008 Reproductive & developmental biology Vol.32 No.3
This study was carried out to develop knock-in vector for EGFP (enhanced green fluorescent protein) expression in porcine β-casein locus. For construction of knock-in vector using porcine β-casein gene, we cloned the β-casein genome DNA from porcine fetal fibroblast cells, EGFP and SV40 polyA signal using PCR. The knock-in vectors consisted of a 5-kb fragment as the 5' recombination arm and a 2.7-kb fragment as the 3' recombination arm. We used the neomycin resistance gene (neor) as a positive selectable marker and the diphtheria toxin A (DT-A) gene as a negative selectable marker. To demonstrate EGFP expression from knock-in vector, we are transfected knock-in vector that has EGFP gene in murine mammary epithelial cell line HC11 cells with pSV2 neo plasmid. The EGFP expression was detected in HC11 cells transfected knock-in vector. This result demonstrates that this knock-in vector may be used for the development of knock-in transgenic pig.
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Analysis of Brain Waves Before and After Plant Cutting Procedure
이상미,김경미,정선희,정순진,한경숙,채영,장윤아,이숙,장현진 인간식물환경학회 2018 인간식물환경학회지 Vol.21 No.5
The purpose of this study was to analyze brain waves before and after the plant cutting procedure in 34 adult subjects. After plant cutting procedure, RT(Relative power of theta) decreased and RB(Relative power of beta), RMB(Relative power of mid beta), RHB(Relative power of high beta), RST(Ratio of SMR to theta spectrum), RMT(Ratio of mid beta to theta), and RSMT(Ratio of SMR~mid beta to theta) increased, while RA(Relative power of alpha), RLB(Relative power of low beta), and RG(Relative power of gamma) did not change significantly. The decrease in the RT indicator means that the cutting did not cause anxiety as a stable work rather than a high level task. Therefore, it is considered that the cutting activity can be applied to the subjects with low cognitive level such as children, dementia, and intellectual disabilities and those who should avoid the psychological burden due to the performance of the work. The increases in RB, RMB, and RHB indicators mean a state of mental awakening, inducing concentration similar to meditation. Therefore, the activity of cutting can be applied to people who use psychological energy in daily life, psychological confusion, excessive unnecessary thought, troublesome problems. The increases in the RST, RMT, and RSMT indicators mean an increase in concentration. Therefore, it is possible to apply the cutting activity for the purpose of strengthening the attention and intensifying induction. The results of this study are meaningful that it was confirmed that the cutting activity could induce the brain to be awakened by inducing concentration through conscious activities without giving an intellectual anxiety or psychological burden.
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Analyzing the Effects between Antecedents, Nurses' Trust in Organization, and Outcome Variables
이상미,김흥국 피터드러커 소사이어티 2015 창조와 혁신 Vol.8 No.3
Despite the significance of trust in organization(TIO) for improving individual well-being and organizational effectiveness, little relevant research has been conducted so far in nursing. Noting the research need, the primary aim of the present study was to examine the causal relationships between nurses' TIO, its antecedents(organizational consideration(OCN), procedural justice(PJ), trust propensity(TP)), and outcome variables(organizational commitment(OC), turnover intention(TI)). The study also aimed to test the moderating effects of nurses' TP on the relationships between OCN and TIO, and between PJ and TIO. Research hypotheses were proposed, given the character-based and relationship -based perspectives on trust. For collecting the data, we conducted a questionnaire survey for the hospital clinical nurses. We tested the hypotheses, using the PLS(Partial Least Squares) statistical package program. The results indicate that OCN, PJ, and TP positively influence nurses' TIO, and that nurses' TIO has significant effects on OC and TI, as hypothesized. Meanwhile, the moderating effect of TP on the relationships between OCN and TIO was rejected, whereas the moderating effect of TP on the relationships between PJ and TIO was supported. The research findings demonstrate that OCN, PJ, and individual TP are important predictors of nurses' TIO, and in turn, OC and TI. The major contribution of the study is that, first, it tested the moderating effect of TP on the relationships between OCN and TIO, and between PJ and TIO, although the hypotheses were partially supported. Second, this study provides considerable theoretical and empirical contributions in that the integrative research model showed a high explanatory power for nurses' TIO, by incorporating OCN and TP as antecedents which were little addressed in prior nursing research. The study results also provide practical implications that innovative system changes in OCN and PJ are needed for enhancing nurses' TIO, and in turn, increasing OC and decreasing TI. Furthermore, we suggested the need for implementing the TP training program in order to enhance nurses' TP.
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‘이완-사회적지지-인지행동’ 전략에 기반 한 원예치료 프로그램이 여성 암환자의 삶의 질, 스트레스, 세로토닌 변화에 미치는 영향 - 실내 활동을 중심으로 -
이상미,최연하,김경미,정선희,전미선,이숙 한국인간·식물·환경학회 2016 인간식물환경학회지 Vol.19 No.4
This study was conducted to evaluate effects of indoor horticultural therapy program based on ‘relaxation - cognitive behavior - social support’ strategy on quality of life, stress and serotonin of woman cancer patients. Subjects were 18 female cancer patients. Experimental group of 9 members received a horticultural therapy program and control group of 9 members did not receive a horticultural therapy program. The horticultural therapy program was conducted, for 90 minutes per session, once a week, total 8 times. And this study was performed from 9. Nov. to 28. Dec. in 2015. As a results, horticultural therapy program made significant improvement in emotional condition among sub-category of quality of life, nervousness, depression and frustration among sub-category of stress. Also the program decreased the total stress score with significant increased level of serotonin comparing to control group. This result means that depression mood in cancer patients was improved. In summary, horticultural therapy program focused on indoor activities based on ‘Relaxation-social support-cognitive behavioral’ strategy could be utilized as supporting program for psychological health for cancer survivors.
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