Evaluation of Signal Stability from Electrochemical Immunoanalysis Based on the Enzyme ‘back-filling’ Immobilization to Biorecognition Interfaces

이준황,원병연,윤현철 한국바이오칩학회 2007 BioChip Journal Vol.1 No.1

The signal stability from electrochemical immunosensors based on the enzymatic back-filling strategy was investigated. The presence of an analyte, especially antibody, which is bound to the surfaceimmobilized capture molecule, could cause a significant signal loss during subsequent handling procedures and signaling due to its limited affinity /stability. Compared to the typical method of detection, the signal from the back-filling immunoassay is generated differently from the covalently immobilized (‘back-filled’) glucose oxidase (GOX) to the sensing surface, not from the bound analyte that is usually labeled with signaling molecules. Therefore, an enhancement of signal stability would be expected with the back-filling immunoassay. To evaluate the merits of the enzymatic back-filling method, various antibody dissociation conditions from immunosensor surfaces were compared in terms of signal stability and sensitivity. As a model immunosensing reaction, the 2, 4-dinitrophenyl (DNP) group was functionalized to the biorecognition surface on a thin-film gold electrode, and anti-DNP antibody was employed as the target analyte. Cyclic voltammetry was used for immunosensor signal registration and to trace the protein adsorption/dissociation process. The back-filling method was found to be suitable for the analysis of immune reactions without labeled-antibody molecules, and the signal stability was comparable to the affinity sensor employing biotin/avidin couple.

흑홍삼혼합물이 체내 알코올대사에 미치는 효과

이준,황병환,송혜진,장선형,최수영,Lee, Jun,Hwang, Byung Hwan,Song, Hye-Jin,Jang, Seon Hyeong,Choe, Soo Young 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.5

본 시험은 흑홍삼이 함유된 혼합물의 숙취해소효능을 확인하기 위해 수행하였다. 효소원을 이용한 혼합 재료들 간의 ADH와 ALDH의 활성은 시중에 판매되고 있는 타사 비교군에 비해 현저히 높은 값을 보였다. 그리고 알코올을 투여하여 숙취를 유도한 시험동물의 혈중 에탄올의 양과 아세트알데히드의 양을 측정하여 생체 내 알코올 분해효능을 평가한 결과, 흑홍삼혼합물을 섭취한 군에서 알코올 투여 1시간 후부터 에탄올의 양은 숙취유도군에 비해 월등히 낮았으며, 시간이 지날수록 농도 의존적으로 에탄올의 양이 감소하였다. 주요 숙취 증상을 야기하는 아세트알데히드의 양은 숙취유도군에서 시간이 지날수록 증가한 반면, 흑홍삼혼합물 섭취군은 알코올 섭취 1시간 후부터 증가하였다가 시간이 지날수록 빠르게 감소함을 확인하였다. 또한 흑홍삼혼합물이 알코올분해효소의 활성에 미치는 영향을 알아보기 위해 간 조직을 분리해 ADH와 ALDH의 활성을 측정한 결과, 흑홍삼혼합물을 섭취한 군이 타사 비교군에 비해 높은 활성을 보임을 확인하였다. 이상의 시험결과로 보아 흑홍삼 혼합음료는 혈중 에탄올의 흡수를 지연시킬 뿐 아니라, ADH와 ALDH의 활성을 증가시켜 혈중 에탄올과 아세트알데히드의 농도를 효과적으로 감소시켜 숙취해소 음료로 이용이 가능하리라 기대된다. Ginsenosides are major constituents of ginseng and are known to be responsible for its pharmacological properties. This study aimed to investigate the detoxification effect of a mixture containing black red ginseng powder, red ginseng extract, Puerariae radix extract, and Hovenia dulcis extract, on SD (Sprague Dawley) rats treated with 30% ethanol. Thirty minutes before treatment, the animals were orally administered different concentrations of the mixture or water. Results revealed that the concentration of ethanol in blood serum was significantly decreased in the black red ginseng mixture treated group in a dose-dependent manner, as compared with that of the control group. The blood level of acetaldehyde increased until 1 hr after alcohol administration, but the levels rapidly decreased later. Furthermore, ADH and ALDH activities in the hepatic tissue were also increased in the black red ginseng mixture administered group, than in the control group. These results indicate that the black red ginseng mixture has the ability of decomposing alcohol by increasing the ADH and ALDH activities responsible for alcohol metabolism.

나노바이오센서기술 개발 랩온어칩(Lab-on-a-chip)을 이용한 나노-바이오센서 기술개발

이준황 ( Jun Hwang Lee ),윤현철 ( Hyun C. Yoon ) 한국공업화학회 2006 공업화학전망 Vol.9 No.2

Electrocatalytic immunosensing on a charge-selective monolayer

Jun Hwang Lee(이준황),Sung Il Jeon(전성일),Jung Woo Hong(홍정우),Seung Yeol  Park(박승열),Hyun C. Yoon(윤현철) 한국생물공학회 2005 한국생물공학회 학술대회 Vol.2005 No.10

Scolopendrasin I: a novel antimicrobial peptide isolated from the centipede Scolopendra subspinipes mutilans

이준하,김인우,김미애,윤은영,남성희,안미영,이영보,황재삼 한국잠사학회 2015 International Journal of Industrial Entomology Vol.31 No.1

In a previous report, we identified several candidate antimicrobial peptides through de novo RNA sequencing of the centipede Scolopendra subspinipes mutilans. Here, we identify and characterize one of these peptides, Scolopendrasin I. We identified the centipede antimicrobial peptide Cecropin from the centipede transcriptome using an SVM algorithm, and subsequently analyzed the amino acid sequence for predicted secondary structure using a GOR algorithm. We identified an alpha helical region of Cecropin and named it Scolopendrasin I. We then assessed antimicrobial and hemolytic activity of Scolopendrasin I. Scolopendrasin I showed antimicrobial activity against various microbes, including antibiotic-resistant Gram-negative bacteria, in a radial diffusion assay. Scolopendrasin I had potent antibacterial activity against acne-associated microbes in a colony count assay and showed no hemolytic activity in a hemolysis assay. In addition, we confirmed that Scolopendrasin I bound to the surface of bacteria via a specific interaction with lipoteichoic acid and lipopolysaccharide, two components of bacterial cell membranes. In conclusion, the results presented here provide evidence that this is an efficient strategy for antimicrobial peptide candidate identification and that Scolopendrasin I has potential for successful antibiotic development

고구마 조기재배시 품종별 생육과 괴근비대 특성

황엄지,남상식,이준설,이형운,양정욱 한국작물학회 2017 한국작물학회지 Vol.62 No.1

To obtain basic data on the effects of differences cultivation periods and methods on growth and development of sweet potato tuber. We investigated growth and development of six major cultivars showing different characteristics, such as dry type(Yulmi, YM; Jinhongmi, JHM), intermediate type(Pungwonmi, PWM; Hogammi, HGM), and purple type(Sinjami, SJM; Danjami, DJM). The average tuber weight of YM and JHM was 4.1 and 8.6 g respectively, 50 days after transplant. At 80 days, the weight of YM and JHM rapidly increased to 25.2 and 26.1 g, respectively. Finally, at 120 days, the weight of YM and JHM increased to 120.7 and 79.4 g, respectively. The average tuber weight of PWM and HGM was 13 and 3.3 g, respectively, 50 days after transplant. The development pattern of PWM showed a consistent increase in tuber weight during the cultivation period, an average weight of 104.2 g at 120 days. However, the tuber development pattern of HGM was different from that of PWM. The average tuber weight of HGM rapidly increased for 100 days after transplant and then showed no increase at 120 days. In case of purple type sweet potato, the average tuber weight of SJM consistently increased during the cultivation period, whereas DJM showed rapid increase in average tuber weight at 80 days after transplant. Korean consumers prefer small, round sweet potato approximately 100g in weight. Based on the results, YM, PWM and SJM are classified as early developing cultivars that can be harvested approximately 120 days after transplant. On the other hand, JHM, HGM, and DJM are classified as late developing cultivars that can be harvested approximately 130 days after transplant.

흰점박이꽃무지 유래 항균 펩타이드 프로테티아마이신 2의 항염증활성

이준하(Joon Ha Lee),백민희(Minhee Baek),이화정(Hwa Jeong Lee),김인우(In-Woo Kim),김선영(Sun Young Kim),서민철(Minchul Seo),김미애(Mi-Ae Kim),김성현(Seong Hyun Kim),황재삼(Jae Sam Hwang) 한국생명과학회 2019 생명과학회지 Vol.29 No.11

흰점박이꽃무지는 약용으로서 매우 유용하고 중요한 곤충종이다. 선행연구를 통해 전사체 분석결과를 바탕으로 인실리코(in silico) 분석을 실시하여 전사체유래 항균 펩타이드를 스크리닝하고 선발하여 항균활성 및 용혈활성을 확인하였다. 그 결과 선발된 항균 펩타이드들은 박테리아와 칸디다 진균에 강한 항균활성을 나타낸 반면 적혈구에 대한 용혈활성은 전혀 없었다. 선발한 펩타이드들 중 프로테티아마이신 2로 명명한 양이온 항균 펩타이드를 이용하여 항균활성뿐만 아니라 마우스의 대식세포 Raw264.7 세포주를 이용하여 프로테티아마이신 2의 항염증활성을 확인하였다. 그 결과 프로테티아마이신 2는 LPS로 유도된 Raw264.7 세포들의 산화질소 생성을 감소시키는 결과를 보여주었다. 또한 프로테티아마이신 2가 산화질소 합성효소(iNOS), 고리형 산소화 효소-2(COX-2)의 발현을 감소시킨다는 것을 실시간 역전사 중합효소 연쇄반응(qRT-PCR) 방법과 웨스턴 블랏(western blot)을 통해 확인하였다. 더욱이 Raw264.7 세포에서 전염증성 사이토카인(TNF-α, IL-6, IL-1β)의 발현이 MAPKs와 NF-κB 신호전달과정을 통해 약화되어짐을 알 수 있었다. 게다가 프로테티아마이신 2가 LPS와 상호작용을 통해 결합한다는 것을 확인하였다. 종합하여 보면 프로테티아마이신 2는 항균활성과 함께 LPS로 유도된 Raw264.7 세포에서 항염증활성도 가지고 있음을 나타내었다. The white-spotted flower chafer Protaetia brevitarsis seulensis is a medicinally beneficial and important edible insect species. We previously performed an in silico analysis of the Protaetia brevitarsis seulensis transcriptome to identify putative antimicrobial peptides and then tested their antimicrobial and hemolytic activities. These peptides had potent antimicrobial activities against bacteria and yeast without inducing hemolysis. In the present study, the cationic antimicrobial peptide, protaetiamycine 2, was selected for further assessment of its anti-inflammatory properties in mouse macrophage Raw264.7 cells. Protaetiamycine 2 treatment of Raw264.7 cells suppressed LPS-induced nitric oxide production and reduced the expression of inducible nitric oxide synthase and cyclooxygenase-2, as determined by real-time PCR and western blotting. The expression of proinflammatory cytokines (TNF-α, IL-6, and IL-1β) was also attenuated through the MAPKs and NF-κB signaling. We also confirmed that protaetiamycine 2 bound to bacterial cell membranes by a specific interaction with LPS. Collectively, these data obtained from LPS-induced Raw264.7 cells indicated that protaetiamycine 2 could have both antimicrobial and anti-inflammatory properties.

UWB 시스템을 위한 1.8V 8-bit 500MSPS 저 전력 CMOS D/A 변환기의 설계

이준흥,송민규,황상훈 대한전자공학회 2006 電子工學會論文誌-SD (Semiconductor and devices) Vol.43 No.12

In this paper, 1.8V 8-bit 500MSPS Low-power CMOS Digital-to-Analog Converter(DAC) for UWB(Ultra Wide Band) Communication Systeme is proposed. The architecture of the DAC is based on a current steering 6+2 full matrix type which has low glitch and high linearity. In order to achieve a high speed and good performance, a current cell with a high output impedance and wide swing output range is designed. Further a thermometer decoder with same delay time and low-power switching decoder for high efficiency performance are proposed. The proposed DAC was implemented with TSMC 0.18um 1-poly 6-metal N-well CMOS technology. The measured SFDR was 49dB when the output frequency was 50MHz at 500MS/s sampling frequency. The measured INL and DNL were 0.9LSB and 0.3LSB respectively. The DAC power dissipation was 20mW and the effective chip area was 0.63mm2. 본 논문에서는 UWB(Ultra Wide Band)통신시스템을 위한 1.8V 8-bit 500MSPS의 D/A 변환기를 제안한다. 전체적인 D/A 변환기의 구조는 높은 선형성과 낮은 글리치 특성을 갖는 상위 6-MSB(Most Significant Bit) 전류원 매트릭스(Current Cell Matrix)와 하위 2-LSB(Least Significant Bit) 전류원 매트릭스로 구성된 2단 매트릭스 구조로 설계하였다. 또한 동일한 지연시간을 갖는 Thermometer Decoder와 고속 동작에서 전력을 최소화하기 위한 저 전력 스위칭 디코더(Current Switching Decoder Cell)를 제안함으로서 D/A 변환기의 고속 동작에서 성능을 향상시켰다. 설계된 DAC는 1.8V의 공급전압을 가지는 TSMC 0.18㎛ 1-poly 6-metal N-well CMOS 공정으로 제작되었으며, 제작된 D/A 변환기의 측정결과, 매우 우수한 동적성능을 확인하였다. 500MHz 샘플링 클럭 주파수와 50MHz의 출력신호에서 SFDR은 약 49dB, INL과 DNL은 각각 0.9LSB, 0.3LSB 이하로 나타났으며, 이 때의 전력소비는 약 20mW로 기존의 8-bit D/A변환기에 비해 매우 낮음을 확인 할 수 있었다. D/A 변환기의 유효 칩 면적은 0.63mm2(900um×700um) 이다.

곤충유래 항균 펩티드의 의약학적 적용

이준하(Joon Ha Lee),김인우(In-Woo Kim),김미애(Mi-Ae Kim),윤은영(Eun Young Yun),황재삼(Jae Sam Hwang) 한국생명과학회 2016 생명과학회지 Vol.26 No.6

현재까지 곤충 항균 펩티드는 1980년에 세크로피아나방(Hyalophora cecropia) 번데기의 혈림프로부터 세크로핀(cecropin)이 처음으로 정제된 이후로 150개 이상의 펩티드가 분리되어 특성들이 보고되어 왔다. 그러므로 곤충은 항균 펩티드 선발을 위한 좋은 재료로서 고려되어 왔다. 곤충 항균 펩티드는 분자량이 작으며 양전하를 띠고 다양한 길이와 서열 및 구조를 갖는 양친매성의 특징을 갖는다. 곤충 항균 펩티드는 박테리아, 진균, 기생충, 그리고 바이러스와 같은 병원체들의 침입에 대항하여 곤충의 선천성 면역체계에서 중요한 역할을 수행한다. 대부분의 곤충 항균 펩티드들은 상처가 나거나 면역화 시 지방체와 다른 특정 조직들에서 유도 합성된다. 이어서 그 항균펩티드들은 미생물들에 대항하여 작용하기 위해 혈림프로 분비되어 나온다. 이들 펩티드들은 항암활성을 포함하여 다양한 미생물들에 대해 광범위한 항균활성을 나타낸다. 곤충 항균 펩티드는 구조 및 서열상의 특징들에 기초하여 크게 4개의 패밀리로 나누어질 수 있다. 다시 말해서 α-나선형 펩티드, 시스테인-풍부 펩티드, 프롤린-풍부 펩티드, 그리고 글리신-풍부 펩티드/단백질이 그것이다. 예를 들면, 세크로핀, 곤충 디펜신(defensin), 프롤린-풍부 펩티드, 그리고 아타신(attacin)이 일반적인 곤충 항균 펩티드들인데, 글로베린(gloverin)과 모리신(moricin)은 나비목 종들에서만 확인되어 왔다. 본 총설에서는 곤충의 항균 펩티드들에 초점을 맞추어 곤충 항균 펩티드들의 적용 가능성 및 방향과 함께 현재의 지식들과 최근의 진전된 사항들에 대하여 논의하고자 한다. By this time, insect antimicrobial peptides (AMPs) have been characterized more than 150 peptides since purification of cecropin in the hemolymph of pupae from Hyalophora cecropia in 1980. Therefore, it is considered that insects are good sources of AMP selection. Insect AMPs are small (low molecular weight) and cationic, and amphipathic with variable length, sequence, and structure. They perform a pivotal role on humoral immunity in the insect innate immune system against invading pathogens such as bacteria, fungi, parasites, and viruses. Most of the insect AMPs are induced rapidly in the fat bodies and other specific tissues of insects after septic injury or immune challenge. Then the AMPs subsequently released into the hemolymph to act against microorganisms. These peptides have a broad antimicrobial spectrum against various microbes including anticancer activities. Insect AMPs could be divided into four families based on their structures and sequences. That is the α-helical peptides, cysteine-rich peptides, proline-rich peptides, and glycine-rich peptides/proteins. For instance, cecropins, insect defensins, proline-rich peptides, and attacins are common insect AMPs, but gloverins and moricins have been identified only in lepidopteran species. This review focuses on AMPs from insects and discusses current knowledge and recent progress with potential applications of insect AMPs.

전신성 홍반성 루푸스 환아에서 병발한 혈구 탐식성 조직구 증식증 1례

황자영,노석만,이진,장필상,김영훈,김진택,이준성,Hwang, Ja Young,No, Suk Man,Lee, Jin,Jang, Pil Sang,Kim, Young Hoon,Kim, Jin Tack,Lee, Joon Sung 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.10

본 저자들은 전신성 홍반성 루푸스와 미만성 증식성 사구체신염으로 진단된 남아의 치료과정 중 발열과 혈구 감소증이 지속된 환아에서 이차성 혈구 탐식성 조직구 증식증을 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Hemophagocytic lymphohistiocytosis is a reactive disorder characterized by a generalized non-malignant histiocytic proliferation with prominent hemophagocytosis by stimulated histiocytes in the bone marrow and reticuloendothelial systems resulting in pancytopenia and liver dysfunction. Several diseases including infection, malignancy and autoimmune disease are known to be causative disorders. This case demonstrated histiocytic hemophagocytosis in the bone marrow, resulting in pancytopenia during treatment of systemic lupus erythematosus and did not show any underlying disease.