Galleria mellonella 6-Tox Gene, Putative Immune Related Molecule in Lepidoptera

이준하,박승미,채권석,이인희 한국잠사학회 2010 International Journal of Industrial Entomology Vol.21 No.1

We have characterized full-length cDNA encoding Gall-6-tox protein, which was cloned from the fat body of the immunized Galleria mellonella larvae. The cloned cDNA of Gall-6-tox consists of 1301 nucleotides and contained an open reading frame of 891 nucleotides corresponding to a protein of 296 residues that includes a putative 16-residue signal sequence and a 280-residue mature peptide with a calculated mass of 30,707.73 Da. The deduced mature peptide contains conserved tandem repeats of six cysteine-stabilized alpha beta (Cs ) motifs, which was detected in scorpion toxins and insect defensins. In the sequence homology search, mature Gall-6-tox showed 34% and 28% amino acid sequence homology with Bomb-6-tox from Bombyx mori and Spod-11-tox from Spodoptera frugiperda, respectively. Gall-6-tox orthologs were only found in Lepidopteran species, indicating that this new immune-related gene family is specific to this insect order. RT-PCR analysis revealed that Gall-6-tox was expressed primarily in the larval fat bodies, hemocytes,and midgut against invading bacteria into hemocoel. Moreover, the expression time course of Gall-6-tox was examined up to 24 h in the fat bodies and midgut after injection of E. coli. Altogether, these results suggest that Gall-6-tox is derived from defensins and Gall-6-tox may play a critical role in Lepidoptera immune system.

그래핀 공진기 기반의 나노 센서에 대한 연구

이준하 한국반도체디스플레이기술학회 2017 반도체디스플레이기술학회지 Vol.16 No.1

Currently, the size of the electronic device is in the nano area. In order to control the movements of these nanoscale devices, one should be able to understand the physical phenomena in the nano area. Recently, due to carbon nanotubes and mechanical outstanding electrical conductivity and mechanical characteristics of the carbon nanotubes and Graphene behaves to apply. Efforts have been active. There are various tubes with a radius of a in a compact mass in the form of a Multi walled carbon nanotubes in different between the radius. Van der Waals force can move smoothly without friction with each other by the nanoscale motor turning, using the properties, making. This is the lightest solids per unit area on the thickness is electrical atomic layer one of the substance and the electrical conductivity, the best material and mechanical characteristics are very much. Many studies because great is the ideal nanoelectromechanical device of material is being considered. In this study, electrical resonator for a new structure proposed and the nature and methodology would like to come up.

전자 부품 검사용 광학분석 장비제어시스템에 대한 연구

이준하 한국반도체디스플레이기술학회 2015 반도체디스플레이기술학회지 Vol.14 No.4

Product of technology developed in this study is an external interface for controlling the equipment of pendant key remote control system circuit board, and it is used in the electronic component test equipment system. Main control system module is in the role as a device for controlling the various control devices that make up the integrated system for microscopic examination at the request of the host computer engineers to control the inspection equipment. The pentane-key interface module to its role as a device for controlling the various control devices that make up the integrated system for microscopic examination at the request of the host computer for the engineer to control the inspection equipment. Development of the control system can be expected in the configuration of a system for efficient and accurate inspection of high-precision parts.

Scolopendrasin I: a novel antimicrobial peptide isolated from the centipede Scolopendra subspinipes mutilans

이준하,김인우,김미애,윤은영,남성희,안미영,이영보,황재삼 한국잠사학회 2015 International Journal of Industrial Entomology Vol.31 No.1

In a previous report, we identified several candidate antimicrobial peptides through de novo RNA sequencing of the centipede Scolopendra subspinipes mutilans. Here, we identify and characterize one of these peptides, Scolopendrasin I. We identified the centipede antimicrobial peptide Cecropin from the centipede transcriptome using an SVM algorithm, and subsequently analyzed the amino acid sequence for predicted secondary structure using a GOR algorithm. We identified an alpha helical region of Cecropin and named it Scolopendrasin I. We then assessed antimicrobial and hemolytic activity of Scolopendrasin I. Scolopendrasin I showed antimicrobial activity against various microbes, including antibiotic-resistant Gram-negative bacteria, in a radial diffusion assay. Scolopendrasin I had potent antibacterial activity against acne-associated microbes in a colony count assay and showed no hemolytic activity in a hemolysis assay. In addition, we confirmed that Scolopendrasin I bound to the surface of bacteria via a specific interaction with lipoteichoic acid and lipopolysaccharide, two components of bacterial cell membranes. In conclusion, the results presented here provide evidence that this is an efficient strategy for antimicrobial peptide candidate identification and that Scolopendrasin I has potential for successful antibiotic development

다중 도메인 데이터 기반 구별적 모델 예측 트레커를 위한 동적 탐색 영역 특징 강화 기법

이준하,원홍인,김병학 대한임베디드공학회 2021 대한임베디드공학회논문지 Vol.16 No.6

Visual object tracking is a challenging area of study in the field of computer vision due to many difficult problems, including a fast variation of target shape, occlusion, and arbitrary ground truth object designation. In this paper, we focus on the reinforced feature of the dynamic search area to get better performance than conventional discriminative model prediction trackers on the condition when the accuracy deteriorates since low feature discrimination. We propose a reinforced input feature method shown like the spotlight effect on the dynamic search area of the target tracking. This method can be used to improve performances for deep learning based discriminative model prediction tracker, also various types of trackers which are used to infer the center of the target based on the visual object tracking. The proposed method shows the improved tracking performance than the baseline trackers, achieving a relative gain of 38% quantitative improvement from 0.433 to 0.601 F-score at the visual object tracking evaluation. Visual object tracking is a challenging area of study in the field of computer vision due to many difficult problems, including a fast variation of target shape, occlusion, and arbitrary ground truth object designation. In this paper, we focus on the reinforced feature of the dynamic search area to get better performance than conventional discriminative model prediction trackers on the condition when the accuracy deteriorates since low feature discrimination. We propose a reinforced input feature method shown like the spotlight effect on the dynamic search area of the target tracking. This method can be used to improve performances for deep learning based discriminative model prediction tracker, also various types of trackers which are used to infer the center of the target based on the visual object tracking. The proposed method shows the improved tracking performance than the baseline trackers, achieving a relative gain of 38% quantitative improvement from 0.433 to 0.601 F-score at the visual object tracking evaluation.

나노전자소자로서의 관성센서 시스템에 관한 연구

이준하,Lee, Jun-Ha 한국반도체디스플레이기술학회 2009 반도체디스플레이기술학회지 Vol.8 No.4

We investigated a nanoscale inertia sensor based on telescoping carbon nanotubes, using classical molecular dynamics simulations. The position of the telescoping nanotubes is controlled by the centrifugal force exerted by the rotation platform, thus, position shifts are determined by the capacitance between carbon nanotubes and the electrode, and the operating frequency of the carbon nanotube oscillator. This measurement system, tracking oscillations of the carbon nanotube oscillator, can be used as the sensor for numerous types of devices, such as motion detectors, accelerometers and acoustic sensors.

Multi-Waled Carbon Nanotube Oscilator Behavior Analysis Using Classical Molecular Dynamics Simulations

이준하,강정원 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.2

The behaviors of multi-walled carbon nanotube (CNT) oscillators were investigated by means of classical molecular dynamics simulations. As the number of walls increases, the number of frequency peaks also increases because the walls are coupled with each other. The frequency corresponding to the maximum peak height of each frequency spectrum for the multi-walled CNT oscillators is less than the frequency for the double-walled CNT oscillators. The highest frequency of the multi-walled carbon nanotube oscillators is greater than the frequency of the double-walled carbon nanotube oscillators. The amplitudes for higher frequencies tend to decrease as the number of walls increases.

주기적 다중 응답 추적 기반의 주행 영상 속도 추론

이준하,원홍인,김민영(Min Young Kim),김병학(Byeong Hak Kim) 제어로봇시스템학회 2022 제어로봇시스템학회 국내학술대회 논문집 Vol.2022 No.6

OLETF 당뇨쥐에서 당뇨시기에 따른 방광의 기능적, 형태학적및 분자생물학적 변화

이준하,서홍진,조혁진,이용석,김현우,김상훈,김세웅,황태곤,이선주,이지열 대한비뇨의학회 2009 Investigative and Clinical Urology Vol.50 No.4

Purpose: Our study was undertaken to investigate changes in the bladder according to duration of diabetes mellitus in the Otsuka Long Evans Tokushima Fatty (OLETF) rat model, which is similar to type 2 diabetes. Materials and Methods: OLETF rats (n=14) and Long Evans Tokushima Otsuka (LETO, n=14) rats were used. LETO is a normal control of OLETF. The animals were assigned to 4 groups: L-40 group, LETO rats 40 weeks after birth (n=7); O-40 group, OLETF rats 40 weeks after birth (n=7); L-60 group, LETO rats 60 weeks after birth (n=7); and O-60 group, OLETF rats 60 weeks after birth (n=7). At 40 weeks or 60 weeks after birth, blood glucose, cystometry, bladder weight, detrusor contractility, and mRNA expression of nerve growth factor (NGF) were assessed. Results: Cystometry showed that diabetic bladders had increased compliance compared with the control groups at 40 and 60 weeks, and the O-60 group had greater compliance than the O-40 group. Contractile responses to electrical stimulation, bethanecol (250μM), and ATP (10 mM) were decreased in the experimental groups compared with the control groups at 40 and 60 weeks, and the O-60 group had a lower contractile response than the O-40 group. The mRNA expression of NGF was decreased in the experimental groups compared with the control groups, and the O-60 group had lower expression than the O-40 group. Changes in NGF were identified through immunohistochemical staining. Conclusions: The degree of diabetic cystopathy in OLETF rats was changed by duration of type 2 diabetes mellitus. Our results showed that the changes in the bladder in type 2 diabetes mellitus can be identified through a new rat model.

OLETF 당뇨쥐에서 당뇨시기에 따른 방광의 기능적, 형태학적 및 분자생물학적 변화 /

이준하,서홍진,조혁진,이용석,김현우,김상훈,김세웅,황태곤,이선주,이지열 大韓泌尿器科學會 2009 Korean Journal of Urology Vol.50 No.4