다층 잠재프로파일분석을 활용한 고등학생의 창의역량 유형: 학생, 학교 수준 영향요인

손윤희,김나영 경북대학교 사범대학부속중등교육연구소 2019 중등교육연구 Vol.67 No.4

The present study explored the latent profiles of high school students' creative competencies and the influential factors at student and school level using the sixth data release of the Korean Educational Longitudinal Study(KELS) 2013. Additionally, we compared the means of student agency according to each creative competency profile. The results showed as follows. First, high school students' creative competency profiles were identified five distinctive profiles: ‘superior group in creative competency’, ‘average group’, ‘low -level creative competency group’, ‘high-level knowledge information-processing competency & low- level creative thinking competency group', and 'inferior group in creative thinking competency'. Second, the latent group membership was found to be statistically influenced by student characteristics(female, prior academic achievement, learning attitudes, academic self-concept and the level of educational expectation), home background factors(household income, parents' academic and emotional support), and school level factors such as instructions based on creative competence and teachers' feedback practices. Third, the students in the superior profile in creative competency tended to have the highest level of student agency. Based on these findings, we discussed the implications to improve high school students' creative competences based on the differential instructions and support.

In vitro에서의 댑싸리하고초의 유방암예방효소 활성에 미치는 영향

손윤희,김미경,박선동,남경수,Shon, Yun-Hee,Kim, Mee-Kyung,Park, Sun-Dong,Nam, Kyung-Soo 대한동의생리학회 2006 동의생리병리학회지 Vol.20 No.6

The effect of water extract from Thesium chinese Turczaninow (TCTW) on proliferation of human breast cancer cells, nitric oxide production, nitric oxide synthase expression, and ornithine decarboxylase activity was tested. TCTW inhibited the growth of both estrogen-independent MDA-MB-231 and estrogen-dependent MCF-7 human breast cancer cells. Lipopolysaccharide-induced nitric oxide (NO) production was significantly reduced by TCTW at the concentrations of 1.0 (p<0.05) and 5.0 mg/ml (p<0.005). Expression of inducible nitric oxide synthase (iNOS) was also suppressed with the treatment of TCTW in Western blot analysis. TCTW inhibited induction of ornithine decarboxylase by 12-O-tetradecanoylphorbol-13-acetate (TPA), a key enzyme of polyamine biosynthesis, which is enhanced in tumor promotion. Therefore, TCTW is worth further investigation with respect to breast cancer chernoprevention or therapy.

Ames 및 umu assay를 이용한 감궁탕의 안전성평가

손윤희,김철호,남경수,Shon Yun Hee,Kim Cheorl Ho,Nam Kyung Soo 한국생명과학회 2005 생명과학회지 Vol.15 No.2

감궁탕의 돌연변이원성을 유무를 알아보기 위해 Salmonella typhimurium TA 98 및 TA100을 이용한 돌연변이 원성 실험에서도 감궁탕은 어느 균에서도 돌연변이원성을 나타내지 않았으며, 이는 S-9 mixture 의해 감궁탕이 대사가 된 후에도 이와 유사한 경향을 나타내었다. 또한, SOS umu test의 경우에서도 $\beta-galactosidase$활성에는 별다른 영향을 미치지 않는 것으로 보아 감궁탕은 돌연변이원성을 일으키지 않는 것으로 판정되었으며 S-9 mixture처리에 의한 대사 후에도 이와 유사한 실험결과가 나타났다. 따라서 감궁탕은 그 자체 및 대사 후에도 DNA에 별다른 영향을 미치지 못하는 비교적 안전한 생약처방으로 여겨진다. Gamgung-tang (GGT) that is included in Gamdu-tang (consists of Glycyrrhizae Radix, black beans) and Gunggui-tang(consists of Angelicae Radix and Cnidii Rhizoma) showed therapeutic effect of autoimmume thyroiditis in the previous reports. GGT was tested for the safety using Ames and umu gene expression mutagenicity tests. In Ames test, Salmonella typhimurium TA98 and TA100 were used to identify mutagenic property, and the number of histidine revertants was measured. In SOS umu test, Salmonella typhimurium TA1535 containing plasmid pSK1002 was used as a test strain, and we monitored the levels of umu operon expression by measuring the $\beta-galactosidase$ activity. Mutagenic activity in any assays we tested was not found. After treating S-9 mixture with GGT, mutagenic activity was also not found. The results of this study suggested that there was no DNA damage and mutagenicity of GGT.

단삼 에탄올추출물이 유방암 예방 및 전이에 미치는 영향

손윤희,조현정,김미경,정은정,남경수 한국생약학회 2007 생약학회지 Vol.38 No.1

Abstract − Ethanol extract from Salvia miltiorrhiza (SME) was tested for breast cancer chemoprevention and metastasis by measuring the activites of cytochrome P450 1A1, aromatase, ornithine decarboxylase (ODC), and matrix metalloproteinase (MMP)-9. SME significantly inhibited cytochrome P450 1A1-mediated ethoxyresorufin O-deethylase (EROD) activity in a dose-dependent manner in a concentration range of 100~1,200 μg/ml (p<0.01). Microsomal aromatase (estrogen synthase) activity was suppressed 54.9%~77.5% by the SME at concentration of 600~1,200 μg/ml. ODC activity induced by 12-O-tetradecanoylphorbol- 13-acetate (TPA) was significantly reduced by the SME 900 and 1,200 μg/ml (p<0.05) in MCF-7 breast cancer cells. In addition, SME (900 and 1,200 μg/ml) markedly inhibited MMP-9 activity, a key role in cancer metastasis. Therefore, SME is worth further investigation with respect to breast cancer chemoprevention or therapy.

Helicobacter pylori에 의한 위세포독성 및 interleukin-8 생성에 미치는 무의 억제효과

손윤희,서정일,박인경,황철원,김철호,남경수,Shon Yun Hee,Suh Jeong Ill,Park In Kyung,Hwang Cher Won,Kim Cheorl Ho,Nam Kyung Soo 한국생명과학회 2005 생명과학회지 Vol.15 No.4

무(한국 품종 및 일본 품종)를 사용하여 H. pylori에 의한 위세포독성 및 IL-8생성에 미치는 영향을 살펴보았다. 그 결과 무는 H. pylori에 의한 위세포독성을 농도의존적으로$(2\~10 mg/ml)$ 억제하였으며 또한 위암세포인 KATO III에서 분비되는 IL-8의 생성을 억제시킴$(5\~10mg/ml)$을 알았다. 이러한 사실은 무가 H. pylori감염에 의한 위점막세포의 손상을 방지할 수 있음을 나타낸다. The efforts of Korean and Japanese radishes on the viability and interleukin (JL)-8 production by Helicobacter pylori were investigated in human gastric epithelial cell. Cell viability was significantly decreased when they were incubated with H. pylori toxin (p <0.05, p<0.01 and p<0.005). Co-incubation with Korean or Japanese radish increased H. pylori toxin-inhibited cell growth in a concentration-dependent manner. The production of IL-8 was greatly increased in H. pylori-infected gastric epithelial cell in concentration- and time-dependent manners. The increased production of IL-8 was significantly inhibited by Korean (p<0.05 and p<0.01) or Japanese (p<0.05) radishes $(5\~10mg/ml)$. These results indicate that Korean and Japanese radishes have protective effects on H. pylori-inhibited cell growth and H. pylori-induced gastric mucosal cell inflammation by suppressing the production of inflammatory cytokine (IL-8) from gastric epithelial cell.

천궁 물추출물이 간암예방효소계에 미치는 영향

손윤희,김한규,남경수 한국생약학회 2003 생약학회지 Vol.34 No.4

천궁이 Phase II 효소 유도와 Ornithine Decarboxylase 활성에 미치는 영향

손윤희,김미경,조현정,남경수,Shon, Yun-Hee,Kim, Mee-Kyung,Cho, Hyun-Jung,Nam, Kyung-Soo 대한동의생리학회 2006 동의생리병리학회지 Vol.20 No.6

Water extract from Cnidii Rhizoma (CRW) was tested for colon cancer chemopreventive activity by measuring the induction of phase II detoxification enzyme activity [quinone reductase (QR) and glutathione S-transferase (GST)] and glutathion (GSH) levels and ornithine decarboxylase (ODC) activity in cultured human colorectal adenocarcinoma HT-29 cells. CRW inhibited cell proliferation in cultured HT-29 cells. CRW induced QR activity in a dose-dependent manner in a concentration range of 0.1${\sim}$5.0 $mg/m{\ell}$. GST activity was also induced with the treatment of CRW in HT-29 cells. In addition GSH levels was increased with CRW. CRW inhibited ODC activity, a key enzyme of polyamine biosynthesis, which is enhanced in tumor promotion. These results suggest that CRW has colon cancer chemopreventive activity by increasing phase II enzyme activity and GSH levels and inhibiting ODC activity in vitro.

별불가사리 렉틴의 복수암에 대한 항암효과

손윤희,전경희,최수정,정시련 영남대학교 약품개발연구소 1998 영남대학교 약품개발연구소 연구업적집 Vol.8 No.-

The lectin from starfish, Asterina pectinifera, was purified and tested for its potential antitumor activity. It was shown to possess considerable toxicity toward various tumor cell lines. Concentration of Asterina pectinifera lectin (APL) at 4 mg/5×10^(5) cells resulted in 28% death of Ehrlich ascites tumor cell. 40% of L929, 60% of A549, and 52% of HeLa cells after 48 hours incubation. Toxicity of APL to L929. Ehrlich ascites, A549, and HeLa cells revealed a reduction in cell viability of approximately 70% at APL concentration of 8 mg/5×10^(5) cells after 48 hours incubation. Administration of APL (100 ㎍/day or 300 ㎍/day) inhibited the growth of Ehrlich ascites cells in vitro. Mice given only Ehrlich cells survived an average of 15±1 (S.E.) days. Mice given Ehrlich cells and 100 ㎍ or 300 ㎍ APL had 58% and 67% survival, respectively, after 20 days. These results suggest that APL has antitumor activity.

재정압박이 지방재정지출에 미치는 영향

손윤희 한국지방재정학회 2018 한국지방재정학회 세미나자료집 Vol.2018 No.3

2008 경남 세계 여성인권대회 기념 3부작 특집방송 성범죄보고서

손윤희 경남연구원 2008 경남발전 Vol.- No.94