Lectins from Marine Shells (VII) : Partial Purification and Characterization of New Lectin from a Top Shell, Chlorostoma argyrostoma lischkei

정시련,손경숙,소명숙,전경희,Chung, See-Ryun,Son, Kyeun-Suk,So, Myung-Suk,Jeune, Kyung-Hee 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.3

밤고둥의 생리식염수용액 추출물은 사랑 및 각종 동물 적혈구를 비특이적으로 응집시켰으며 림프구도 응집시켰다. 밤고둥의 새로운 렉틴 성분을 생리식염수용액추출, 황화암모늄 침전, 이온교환 크로마토그래피 등의 방법으로 분리 정제하였다. 이는 전기영동상에서 하나의 주된 띠와 다른 몇개의 띠를 나타내었으며 주된 띠에서만 렉틴활성을 나타냈다. 부분 정제된 밤고둥 렉틴은 pH변화에는 비교적 안정하였으나 열에는 불안정하였다. 적혈구 응집은 lactose와 D-galactose의 각각 10mM과 100mM농도에서 저해되었다. 또한 밤고둥 렉틴은 마우스의 림프구에 대한 분열자극효과를 나타냈다. The whole body extracts of a top shell, Chlorostoma argyrostoma lischkei, agglutinated nonspecifically human and other animal erythrocytes and also agglutinated rat and murine splenic lymphocytes. New lectin was purified by the following procedures: 0.15M NaCl extraction, salt fractionation and ion exchange column chromatographies. The purified lectin showed one major and a few minor bands in polyacrylamide gel electrophoresis, but in only one major band had lectin activity. The partially purified lectin was relatively stable at various pH, but heat labile. Among the tested sugars, only lactose and D-galactose inhibited lectin activity at a concentration of 10mM and 100mM, respectively. The lectin was a mitogenic toward murine splenic lymphocytes

천연물에서 얻은 렉틴 성분과 이 물질의 생명과학에의 응용

정시련,전경희 영남대학교 약품개발연구소 1994 영남대학교 약품개발연구소 연구업적집 Vol.4 No.-

해양 천연물로부터 면역기능 조정제 렉틴 개발 : MLA-Ⅰ, MLA-Ⅱ, MLA-Ⅲ의 특성 Characteristics of the MLA-Ⅰ, MLA-Ⅱ and MLA-Ⅲ

정시련,김장환,전경희 영남대학교 약품개발연구소 1995 영남대학교 약품개발연구소 연구업적집 Vol.5 No.-

Three new lectins. MLA-Ⅰ, MLA-Ⅱ and MLA-Ⅲ, have been isolated and purified from the hemo-lymph of Meretrix lusoria and reported previously. Biophysicochemical characteristics were investigated with these three MLA lectins. The MLA lectins agglutinated human erythrocytes non-specifically and proved as D-galactose group carbohydrate specific. Molecular weight of MLA-Ⅰ, Ⅱ and Ⅲ were estimated to be 330, 500 and 310KD, respectively, by gel filtration on Sepharose CL-6B column. On SDS-polyacrylamide gel electrophoresis. MLA-Ⅰ was dissociated into a single subunit of 42KD. MLA-Ⅱ was into the twelve subunits of 46, 32, 30, 28, 25, 23, 22, 20, 19, 16, 15, and 14KD, and MLA-Ⅲ was into the two subunits of 72 and 44KD. The pI of MLA-Ⅰ, Ⅱ, Ⅲ were 4.0, 4.9 and 5.0. Amino acid analysis revealed a high contents of acidic and hydroxy amino acids. and a paucity of sulfur containing amino acids. Proline was not contained in MLA-Ⅱ.

한국산 식물 자원으로 부터 새로운 렉틴 성분의 분리 정제 (I) : 콩과 식물의 렉틴

정시련,전경희,Chung, See-Ryun,JeuneChung, Kyung-Hee 생화학분자생물학회 1981 한국생화학회지 Vol.14 No.3

In order to find new lectins from Korean natural products, 55 kinds of plants belonging to 31 families were screened by using several different type of blood cells. Phaseolus vulgaris C(W. K. B.) and Cercis chinenesis of Leguminosae contained strong hemagglutinating proteins. The crude lectins from W. K. B. were purified by DAEA Sephadex A-50 column chromatography. The 0.1 M peak from this procedure demonstrated acceptable criteria, e.g. yield, optical density and hemagglutinating activity and three bands were observed in polyacrylamide disc gel electrophoresis. A further purification was achieved by hydroxyapatite column chromatography and strong hemagglutinating activity was recovered in 0.1 M peak. It was elecrophoresed and found to migrate as a single band. The condition of purification for W. K. B. lectin was encourageable but did not brought agreeable satisfaction to the Cercis chinensis lectin. 한국산 식물 자원으로 부터 새로운 렉틴 성분을 개발하고 저 55종의 식물 자원을 대상으로 연구한 결과를 토대로 본 연구에서는 16종의 콩과식물 중 개발 가능성이 큰 Phaseolus vulgaris C와 Cercis chinensis로 부터 이 성분의 분리 정제를 시도하였다. DEAE-Sephadex A-50 column과 hydroxyapatite column으로 분리 정제를 하였고, polyacrylamide disc gel electrophoresis, UV spectrophotometry, 그리고 적혈구와의 agglutinating activity등으로 분리 정제된 렉틴을 비교 검토하였다. 본 연구에서 Phaseolus vulgaris 렉틴은 Cercis chinensis 렉틴에 비해 분리 정제 효과가 대단히 양호하였음을 관찰 하였다.

상용 한약의 항균효과

정시련 약사공론 1993 대한약사회지 Vol.1993 No.2

해양패류렉틴 ( Ⅶ ) 밤고둥 ( Chlorostoma argyrostoma lischkei ) 렉틴의 부분 정제 및 특징

정시련,손경숙,소명숙,전경희 ( See Ryun Chung,Kyeung Suk Son,Myung Suk So,Kyung Hee Jeune - Chung ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.3

The whole body extracts of a top shell, Chlorostoma argyrostoma lischkei, agglutinated nonspecifically human and other animal erythrocytes and also agglutinated rat and murine splenic lymphocytes. New lectin was purified by the following procedures: 0.15M NaCl extraction, salt fractionation and ion exchange column chromatographies. The purified lectin showed one major and a few minor bands in polyacrylamide gel electrophoresis, but in only one major band had lectin activity. The partially purified lectin was relatively stable at various pH, but heat labile. Among the tested sugars, only lactose and D-galactose inhibited lectin activity at a concentration of 10mM and 100mM, respectively. The lectin was a mitogenic toward murine splenic lymphocytes.

생약, 천연물 연구 30년 : 렉틴 연구 25년

정시련 영남대학교 약품개발연구소 2002 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-

사이토카이닌과 식물 라이보좀과의 분자 결합

정시련,베르나 듀랑 ( See Ryun Chung,Bernard Durand ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.3

The binding interactions between cytokinin analogs and ribosomes of a dioecious plant are studied. Molecular interactions between cytokinins and ribosomes have been investigated and compared to male and female by using radioactively labeled 6-benzylamino [8-^(14)C] purine as a probe. Further results on the binding of benzylaminopurine to male ribosomes in connection with kinetin, 6-furfurylaminopurine, and their respective ribosides are reported presently.

한국산 식물 자원으로 부터 새로운 렉틴 성분의 분리 정제 ( 1 ) 콩과 식물의 렉틴

정시련,전경희 ( See Ryun Chung,Kyung Hee Jeune Chung ) 생화학분자생물학회 1981 BMB Reports Vol.14 No.3

In order to find new lectins from Korean natural products, 55 kinds of plants belonging to 31 families were screened by using several different type of blood cells. Phaseolus vulgaris C(W, K. B.) and Cercis chinenesis of Leguminosae contained strong hemagglutinating proteins. The crude lectins from W. K. B. were purified by DAEA Sephadex A-50 column chromatography. The 0.1 M peak from this procedure demonstrated acceptable criteria, e. g. yield, optical density and hemagglutinating activity and three bands were observed in polyacrylamide disc gel electrophoresis. A further purification was achieved by hydroxyapatite column chromatography and strong hemagglutinating activity was recovered in 0. 1 M peak. It was elecrophoresed and found to migrate as a single bans. The condition of purification for W. K. B. lectin was encourageabie but did not brought agreeable satisfaction to the Cercis chinensis lectin.

해양패류로부터 렉틴성분 개발연구 조각매물고둥 렉틴의 분리 , 정제 및 특성

정시련,김장환,전경희 ( See Ryun Chung,Jang Hwan Kim,Kyung Hee Jeune - Chung ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.4

A New lectin from shellfish, Neptunea intersculpta, was purified by the following steps; 0.15 M NaCl extraction, (NH₄)₂SO₄ precipitation, DEAE-cellulose, hydroxyapatite and gel filteration on Sephadex G-100 column chromatography. The NIA (Neptunea intersculpta)lectin was shown one major band and two faint minor bands on polyacrylamide gel electrophoresis. Agglutinating activity was specifically inhibited by lectose and no carbohydrate was found by anthrone test and thin layer chromatography. Immunochemical techniques were employed to examine structural similarities between NIA lectin and other crude lectin. The result of immunodiffusion showed that NIA lectin was partialy identified with white kidney bean crude lectin, but was not identified with Agaricus bisporus crude lectin. Further characterization of the biological and chemical properities of this lectin are still in progress.