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황철원,신현길,도명술,김윤주,박종흠,최영섭,주우홍 Institute of Genetic Engineering Changwon National 2001 Gene and Protein Vol.5 No.-
포항 지역에서 생산되는 부추를 중심으로 부추의 식품기능성 분석을 한 결과 암예방 지표 효소인 QR의 유도능을 확인하였고 항산화 효소의 유도능은 음성대조구에 비해 약 2배 이상의 활성을 보여 앞으로 더 많은 연구를 기대하게 한다. 지역에 따른 QR 유도능과 SOD 유사 활성 유도능의 차이는 각 지역의 토질과 환경에 의한 차이에 기인하는 것으로 판단된다. 이에 대한 구체적인 연구는 토양분석 등 기후 특성에 대한 연구가 이루어져야 할 것으로 사료된다. 부추의 항비만기능성 식품으로서의 가능성을 지방세포 분해능에 의해 측정한 결과 50㎍/㎕ 농도에서는 음성 대조구의 약 3배 이상의 분해능을 나타내어 항비만 기능성 식품으로서 효과가 큰 것으로 기대된다. Leek(Allium tuberosum Rotter) is a korean traditional herb of which leaves are used for daily food. In order to improve the potency of leek as a functional food, we investigated the several biochemical functions in vitro. The quinone reductase activity, and anticarcinogenic enzyme, was induced 2 times at the concentration of 1㎎/㎖ of leek as compared to that of negative control(80% methanol). The induction of SOD(Superoxide dismutase) activity was also demonstrated. But the functional activities of leek were shown to be a little difference depending on the place where the leek was produced. The difference might to be due to the different environmental factors where affects the growth of leek. In an experiment to test the lypolytic activity of leek, the glycerol release was significantly increased(about 3 times to that of negative control). This was suggested that leek can be considered as a potential food for diet. The above results provide the potential food, and more profound research is requested.
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황철원,송일석,김세광,오천환,김태현,정병환,박은혜,김종수,최금찬 한국환경보건학회 2019 한국환경보건학회지 Vol.45 No.5
Objectives: This study was performed to investigate the emission characteristics of aldehydes emitted by the printing industry and to evaluate their impact on adjacent residential areas. Methods: Aldehydes and THC were measured from the front of the control device in the printing and coating process. Aldehydes and ketones were measured by workplace area, residential area, and background area to evaluate their impact. Results: The concentrations of formaldehyde (<0.047 ppm) and acetaldehyde (<0.068 ppm) in the printing and coating process were relatively low, and the methyl ethyl ketone used as the primary solvent was the main carbonyl compound in the printing process. The daily mean concentrations of formaldehyde and acetaldehyde were not significantly different by workplace area, residential area, or background area. However, the concentration of methyl ethyl ketone was high in the order of workplace area, residential area, and background area. Conclusions: The concentrations of formaldehyde and acetaldehyde in the adjacent residential areas are considered to be more influenced by secondary sources of photochemical reactions than by primary sources. Methyl ethyl ketone is considered to be highly influenced by the primary source, which is printing facilities.
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기름으로 오염된 토양에서 작물생육을 위한 계면활성제 생산 Bacteria의 활용에 관한 연구
황철원,장혜원,최용락,Hwang, Cher-Won,Chang, Hae-Won,Choe, Yong-Rak 한국생명과학회 2007 생명과학회지 Vol.17 No.7
Bacillus LPO3 (계면활성제와 같이 유화물질을 생산하는)을 hydrocarbon을 감소시키는 bio-control agent로 사용했다. 토양 (시중구입)은 gasoline을 섞어 오염시킨 토양을 사용하였다. 13일 동안 bacteria의 성장, hydrocarbon의 감소, 그리고, Bacillus LP03 의 작물 성장에 미친 요인(식물의 싹과 뿌리의 길이를 포함한)을 관찰하였다. 우리는 이 bacteria의hydrocarbon을 감소 시키는 물질로서 이 균이 생산하는 계면활성제일 것이라는 가능성을 이미 확인 하였으며 이는 식물의 싹과 뿌리의 생장을 촉진하였고, 회색 곰팡이에 대하여항 진균 활성을 보였다. 본 연구에서는 bacteria를 넣지 않은 기름으로 오염된 토양 실험 군에서는 식물이 자라나지 않았을 뿐 아니라 살아남지도 못했다. 실험 결과로 볼 때, gasoline의 hydrocarbon이 시간이 지남에 따라 감소되었고, RNA blotting실험에서는 오염된 토양에서 균의 증식과 더불어 계면활성제관련 유전자 산물의 증가를 확인하였다 결과적으로, 이 bacteria (계면활성제를 생산하는)는 회색 곰팡이에 감염된 토양이나 기름으로 오염된 토양에서 작물이 자랄 수 있게 하기 위한 bio-control agent로서 유효한 균으로 생각된다. Bacillus sp.LPO3 (producing emulsifying substances such as bio-surfactant) was used as a bio-control agent to degrade hydrocarbon (gasoline in oil spilled crop soil). The soil (brought from fertilizer store)was mixed with gasoline-spilled soil (made with Diatomaceous Earth, Sigma.U.S.A). The study was conducted for a period of 13 days, 13 days during which bacterial growth, hydrocarbon degradation and growth parameters of Bacillus sp.LP03 including shoot and root length were studied. We found that the effective of bacterial producing substance might bio-surfactants let the plants survive even more promote the growth of shoot and root length and showed antifungal activity against gray mold. Without the bacteria, they couldn't grow in oil-spilled soil not even survive. According to the results of the above experiments, we can see with following results, hydrocarbon in gasoline was reduced, day by day, then RNA dot blotting was done and it fit the results we had done. Finally, this Bacteria(producing bio-surfactant) were found to have effective bio-control agent for cropping in oil spilled soil and infected by gray mold.
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토양에서 분리된 Xanthomonas sp. 의 Chitinase 유전자 cloning 과 E.coli 에서의 발현
황철원,김호상,성기영,은무영 ( Cher Won Hwang,Ho Sang Kim,Ki Young Seong,Moo Young Eun ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.2
Xanthomonas sp. isolated from soil exhibited cell wall lytic activity of Candida albicans and secreted chitinase in chitin media. Especially, the chitinase activity was induced by chitin and reached a maximum level at 3 days culture in chitin media. We constructed genomic library of Xanthomonas sp. using cosmid vector in E. coli. Oligonucleotide probe, was synthesized from the consensus sequence corresponding to chitinase active site, which was derived from the comparison of amino acid sequences of bacterial chitinase genes. Using this oligonucleotide probe, we screened the genomic library. By restriction enzyme mapping of the positive clones, we identified 4 independent clones which may contain the chitinase gene. One of the clones, named pXCH1 (1.2 kb insert), was further analyzed. Northern blot analysis indicated that is transcripts, 1 kb and 0.8 kb, were induced by chitin. When the cloned gene was induced by IPTG in E.coli cell, chitinase activity which was secreted onto culture media was not observed. However, when the cell was disrupted by using sonicator and then centrifuged, the supernatant exhibited chitinase activity. SDS-PAGE of the supernatant indicated that about 35 kDa protein was induced by IPTG. From these results, it was concluded that the cloned DNA was one of the chitinase genes of Xanthomonas sp.
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항 곰팡이 단백질 유전자 분석에 의한 국내 무 품종간 유연성에 관한 연구
황철원,Hwang, Cher-Won 한국생명과학회 2007 생명과학회지 Vol.17 No.9
국내 시판 무 (Baekwoon) 의 씨앗으로부터 항 진균 단백질들을 (RAP-l,2)분리 하였으며[12] 이들 항 진균 단백질을 MALDI-TOF실험결과, 2S storage albumin, Rs-AFP등 지하부 식물의 defensin protein과[15] 일치함을 확인하였고 이에 시판되는 7종의 각각의 무 씨앗으로부터 조 단백질과 Total RNA를 분리 하여 항 효모 (Saccharomyces cerevisiae, Candida albicans.) 및 항 곰팡이 (Botrytis cenma)에 대한 항 진균성을 실험한 결과 항 곰팡이 활성은 모든 품종에서 보였으나 항 효모 활성은2 종 (Myungsan, Baekwoon) 의 무에서만 보였 다 . 또한 기존에 알려진 항 진균 단백질 (Rs-AFP)의 유전자를 Gene Bank/EMBL 에서 획득하여 씨앗으로부터 분리한 Total RNA 에 RT-PCR 한결과, 7종 중 2종은 0.2kb 의 산물이 보이지 않았다. 이들 Ks-AFP 유전자산물을 염기서열을 분석하였으며 이 염기서열에서 얻어진 아미노산 서열을 Clustal W를 이용한 pairwise alignment 분석에 의해 국내 시판 무 의 품종간 각clone의 계통수를 분석한 결과를 보고한다. To define diversity of domestic radish, we analysis genetic relationship of anti-fungal protein genes from several domestic radish (Raphanus sativus L.) seeds. We have isolated from domestic radish (Baekwoon) anti-fungal protein named RAP[12]. In this report, we isolate RNAs and raw protein from radish seeds then, RT-PCR analysis was done with another known anti-fungal sequences of radish from Gene Bank/EMBL and anti-fun- gal, anti-yeast activity were done against Bot교tis cenerea, Saccharomyces cerevisiaeι Candida albicans with it's raw proteins. The anti-fungal activity was shown used all seeds but anti-yeast activity was shown only two seeds (Myungsan, Baekwoon). RT-PCR products (about 0.2 Kb) were not shown only two seeds. To identify the sequencing relationship of the domestic radish, we have cloned and sequenced RAP genes of the radish and analysis the sequence relationship with clustalw program. Thus we report the result that there are some different relationship between domestic radish and known other radish's anti- fungal protein[15].
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