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내열성 한천분해효소를 생산하는 해양세균의 분리 및 특성
박근태,이동근,김남영,이어진,정종근,이재화,허문수,이정현,김상진,이상현,Park Ceun-Tae,Lee Dong-Ceun,Kim Nam Young,Lee Eo-Jin,Jung Jong-Ceun,Lee Jae-Hwa,Heo Moon-Soo,Lee Jung-Hyun,Kim Sang-Jin,Lee Sang-Hyeon 한국생명과학회 2005 생명과학회지 Vol.15 No.6
제주도 동북해역의 해수에서 한천분해활성을 보어는 해양성 세균을 분리하였으며 16S rDNA유전자 염기서열분석과 형태학적, 배양적 및 생화학적 특징을 조사하여 해양기원의 Agarivorans 속에 속하는 균주임을 확인하고 Agarivorans sp. JA-1으로 명명하였다. Agarivorans sp. JA-1이 생성하는 한천 분해효소(agarase)는 한천의 존재유무에 상관없이 발현되며 성장의존성인 것으로 확인되었고 효소활성을 위한 최적 pH는 pH 8.04 (50 mM glycine NaOH 완충용액)이고 최적 온도는 $40^{\circ}C$로 나타났다. 한천분해효소는 기능성올리고당 생산이 가능한 베타-한천분해효소이며 내열성을 보여 $60^{\circ}C$까지 $80\%$ 이상 그리고 $80^{\circ}C$까지 $70\%$의 잔존활성을 보이는 것으로 나타났다. 분리한 Agarivorans sp. JA-1 균주가 나타내는 한천분해효소를 이용하여 $40^{\circ}C$ 이상에서 액체상태로 있는 한천을 이용한 기능성올리고당 생산에 유용한 것으로 생각되었다. An agar-degrading bacterium was isolated from north-eastern sea of Jeju island and cultured in marine agar 2216 media. Biochemical and morphologicl characteristics and 165 rRNA gene revealed that isolated strain was member of Agarivorans genus, and named Agarivorans sp. JA-1. Agarase was produced as growth-related and expressed regardless of agar presence. Optimal pH was 8 at 50 mM Clycine-NaOH buffer, and activity was maximum at $40^{\circ}C$E Enzymatic activity was maintained over $80\%$ at $60^{\circ}C$t and $70\%$ at $80^{\circ}C$ which is thermotolerant. Hence isolated novel Agarivorans sp. JA-1 strain and its beta-agarase could be used for the production of functional oligosaccharide from agar in solution state.
국내 연안에서 분리한 Vibrio vulnificus의 특성
박근태,박민정,정초록,송춘복,이제희,여인규,전유진,허문수,Park Geun-Tae,Park Min Jung,Jung Cho-Rok,Song Choon-Bok,Lee Jehee,Yeo In-Kyu,Jeon You Jin,Heo Moon-Soo 한국생명과학회 2004 생명과학회지 Vol.14 No.6
2002년 5월에서 7월사이 한국 근해로부터 총 가검물 50례 중에서 V. vulnificus은 5균주가 분리되었고, API 20E kit의동정률이 $89.4\~93.7\%$로 나타났다. V. vulnificus 16S rDNA를 증폭하여 얻은 산물을 cloning하여 염기서열을 결정하고 분석한 결과 V. vulnificus로 확인되었다. 동정된 분리균주들의 cell lysates을 SDS-PAGE로 분석하였고 표준균주로 사용된 V. vulnificus ATCC 27562와 분리 균주들을 비교해 본 결과 단백밴드pattern이 많은 차이를 보였으나 외막단백질은 V. vulnificus간에는 공통의 밴드가 확인되었으나 V. parahaemolyticus 와는 차이를 보였다. Five strains of Vibrio vulnificus, which cause serious septicemia in human worldwidely, was isolated from marine environments of Korea costal area from May to July of 2002. The isolated strains were identified by API 20E kit and partial 16S rDNA sequence analysis. 16S rDNA sequence of the isolates showed $99\%$ similarity with V. vulnificus ATCC 27562. The proteins of V. vulnificus isolates were examined by analyzing patterns of the cell lysates and outer membrane proteins (OMP). The OMP separated from cell lysates showed the common protein band. Therefore OMP profiles might be useful for the identification of V. vulnificus sp.
페놀분해세균인 Pseudomonas sp. EL-04J로부터 Trichloroethylene 분해효소의 확인
박근태,김호성,손홍주,이건,박성훈,이상준,Park, Geun-Tae,Kim, Ho-Sung,Son, Hong-Ju,Lee, Gun,Park, Sung-Hoon,Lee, Sang-Jun 한국생명과학회 2002 생명과학회지 Vol.12 No.5
Pseudomonas sp. EL-041는 페놀로 적응된 활성오니로부터 분리되었으며, 페놀을 분해할 수 있는 동시에 trick-loroethylene(TCE)을 공동대사할 수 있는 세균이다. 본 연구에서는 Pseudomonas sp. EL-041에서 발현되는 trick-loroethylene 분해효소의 종류를 효소활성 측정 및 specific phenol oxygenase gene의 DNA sequencing을 통하여 조사하였으며, 그 결과 본 균주의 trichloroethylene 분해효소는 monooxygenase였으며, phenol hydroxylase로 추정되었다. Pseudomonas sp. EL-041 was previously isolated from phenol-acclimated activated sludge. This bacterium was capable of degrading phenol and cometabolizing trichloroethylene (TCE). In this study, we report the identification of trichloroethylene- degrading enzyme in Pseudomonas sp. EL-041 by the investigation of enzyme activity and DNA sequencing of specific phenol oxygenase gene. As the results of experiment, trichloroethylene-degrading enzyme in Pseudomonas sp. EL-041 was monooxygenase and suspected to phenol hydroxylase.
박근태,문경환,김형태,박찬정,정호철,임영희,Park, Keun-Tae,Moon, Kyong-Whan,Kim, Hyung-Tae,Park, Chan-Jung,Jeong, Ho-Chul,Lim, Young-Hee 한국환경보건학회 2011 한국환경보건학회지 Vol.37 No.4
Objectives: Respiratory virus infections are the most common disease among all ages in all parts of the world and occur through airborne transmission. The purpose of this study was to detect and quantitate human respiratory viruses in residential environments. Methods: Air samples were collected from the residential space of apartments in the Seoul/Gyeonggi-do area. The samples were collected from indoor and outdoor air. Among respiratory viruses, influenza A virus, influenza B virus, parainfluenza virus, metapneumovirus, respiratory syncytial virus, and adenovirus were investigated by multiplex polymerase chain reaction. Among the virus-positive samples, we performed adenovirus quantification by real-time polymerase chain reaction. Results: Virus detection rates were 44.0%, 3.8%, 3.4%, and 17.3% in spring, summer, autumn, and winter, respectively. The virus detection rate was higher in winter and spring than in summer and autumn. Adenovirus was most commonly detected, followed by influenza A virus and parainfluenza virus. Virus distribution was not significantly different between indoor and outdoor environments. Conclusions: Although virus concentrations were not high in residential environments, residents in houses with detected viruses may have an increased risk of exposure to airborne respiratory viruses, especially in winter and spring.
노심보충수탱크의 직접접촉응축에 대한 MARS의 계산능력평가
박근태(Keun Tae Park),박익규(Ik Kyu Park),이승욱(Seung Wook Lee),박현식(Hyun Sik Park) 한국전산유체공학회 2014 한국전산유체공학회지 Vol.19 No.1
This study aimed at assessing the analysis capability of thermal-hydraulic computer code, MARS for the behaviors of the core make-up tank (CMT). The sensitivity study on the nodalization to simulate the CMT was conducted, and the MARS calculations were compared with KAIST experimental data and RELAP5/MOD3.3 calculations. The 12-node model was fixed through a nodalization study to investigate the effect of the number of nodes in the CMT (2-, 4-, 8-, 12-, 16-node). The sensitivity studies on various parameters, such as water subcooling of the CMT, steam pressure, and natural circulation flow were done. MARS calculations were reasonable in the injection time and the effects of several parameters on the CMT behaviors even though the mesh-dependency should be properly treated for reactor applications.
상백피 추출물을 효소전환 하여 생성한 oxyresveratrol 및 oxyresveratrol-3-O-glucoside의 피부 자극성 및 감작성 평가
박근태(Keun-Tae Park),김정근(Jeong-Keun Kim),임영희(Young-Hee Lim) 한국식품과학회 2012 한국식품과학회지 Vol.44 No.2
상백피 추출물의 연구결과로는 항산화효과, 미백, 주름개선 등의 효과가 있는 것으로 확인되었으나 화장품원료로 사용하기 위한 피부자극, 감작연구는 이루어지지 않은 상태이다. 본 연구에서는 guinea pig를 이용하여 피부 자극시험을 평가한 결과 OXY 및 OXY-3 처리군 모두에서 P.I.I 수치 0.5 이하에 해당하는 비자극성으로 판정되었다. 또한 GPMT법을 이용한 피부감작 시험 평가 결과 OXY 및 OXY-3 처리군 모두에서 발적, 홍반, 부종 등이 전혀 관찰되지 않았고, 반응의 빈도도 0%로 나타나 알러지 유발능이 없는 것으로 판명되었다. 결론적으로, 본 시험조건하에서 guinea pig에 대한 상백피 유래 미백성분들은 1% 이하의 농도에서 사용시 무자극성이며 면역반응에 의한 감작성 부작용을 유발하지 않는 것으로 판단되므로 1% 이하의 시료 물질을 화장품 원료 등에 적용시 약물에 의한 피부 자극성 및 면역반응에 의한 피부 감작성 부작용이 나타날 가능성은 비교적 낮을 것으로 판단된다. Stilbenes are known as antioxidants and some of them demonstrate anti-pigmentation activity. The purpose of the present study was to investigate whether two stilbene compounds produced by biotransformation of the extract of Morus alba root show skin irritation and sensitization. In skin irritation test, 1% oxyresveratrol (OXY), and 5% OXY, and 1% oxyresveratrol-3-O-glucoside (OXY-3) showed a P.I.I score of 0, 0.04, and 0, respectively. Accordingly, the two stilbenes were evaluated to be virtually ‘non-irritant’ materials. In a skin sensitization study by GPMT, 1% OXY, 5% OXY, and 1% OXY-3 did not cause edema and erythema at 24 h and 48 h after topical application and exhibited a sensitization score of 0 and a rate of 0%. Consequently, it was confirmed that OXY and OXY-3 had no contact allergic sensitization in GPMT. Therefore, OXY and OXY-3 might be potential candidates as skin-whitening agents without posing any serious side effects.