방광의 소세포 신경내분비 암종의 요 세포학적 소견 - 1 예 보고 -

김동훈,강동욱,김경희,김주헌,박미자,Kim, Dong-Hoon,Kang, Dong-Wook,Kim, yuug-Hee,Kim, Ju-Heon,Park, Mee-Ja 대한세포병리학회 2002 대한세포병리학회지 Vol.13 No.2

We report the cytologic features of a case of primary small cell carcinoma of the urinary bladder with high grade transitional cell and signet ring cell carcinomatous components. A 64-year-old male presented with gross hematuria for one week. Computed tomography revealed an ill-defined mass in the left lateral wall of the urinary bladder. Urinary cytology showed hypercellularity with predominantly isolated single cells and clustered cells. They have scanty cytoplasm and naked hyperchromatic nuclei with finely granular nuclear chromatin and rare nucleoli. The tumor cells occurred predominantly singe cells, but a few in clusters. Nuclear molding was prominent. No glandular formation or nesting was noted. The second tumor cells had high nuclear/cytoplasmic ratio, irregular nuclear membrane, and coarse granular chromatin. The background was inflamed and necrotic. The histoiogic findings of transurethral resection were mainly composed of small cell carcinoma, and partly transitional cell and signet ring cell carcinomatous components. Small cell neuroendocrine carcinoma have distinctive cytologic features to make a proper diagnosis.

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF)의 첨가가 생쥐 수정란의 발생과 착상관련 유전자 발현에 미치는 영향

김동훈,고덕성,이회창,이호준,강희규,김태전,박원일,김세웅,Kim, Dong-Hoon,Ko, Duck-Sung,Lee, Hoi-Chang,Lee, Ho-Joon,Kang, Hee-Gyoo,Kim, Tai-Jeon,Park, Won-Il,Kim, Seung-Samuel 대한생식의학회 2002 Clinical and Experimental Reproductive Medicine Vol.29 No.2

Objective : The purpose of the current series of experiments were to assess the effect of GM-CSF, as a medium supplement, on the development of mouse embryos and the expression of LIF and IL-1? mRNA. Materials and Methods: Mouse 2-cell embryos were collected from the oviducts of 6 weeks old ICR mice at 48 hours after hCG injection. Embryos were cultured in P-1 medium supplemented with mouse GM-CSF (0, 1, 5, 10 ng/ml). The embryo development to blastocysts and hatching blastocysts was assessed and the cell number in blastocyst was also examined. Using RT-PCR, the expressions of LIF and IL-1? mRNA in blastocyst were evaluated in the GM-CSF supplemented group and control group. Results: In mouse, the addition of GM-CSF increased the percentage of blastocysts (65.5%, 68.6%, 73.0% and 76.1% for control and 1, 5 and 10 ng/ml, respectively), and increased the proportion of hatching blastocysts (35.2%, 36.4%, 43.2% and 53.0% for control and 1, 5 and 10 ng/ml, respectively). The mean cell numbers in blastocyst were significantly increased in GM-CSF supplemented groups compared to control group. LIF and IL-1? expression in blastocyst were significantly higher in GM-CSF supplemented group than in control group. Conclusion: The results of experiment by mouse embryos showed beneficial effects of GM-CSF as a medium supplement. Furthermore, the addition of GM-CSF significantly increased the expression of LIF and IL-1? in mouse embryos. These results suggest that GM-CSF might be a important molecule in embryo implantation.

리튬이온이차전지용 LiCoO₂-유기전해액의 충전상태에 따른 열적 안정성

김동훈,이영호,신혜민,정영동,도칠훈,진봉수,김현수,문성인,오대희,김기원,Kim, Dong-Hun,Lee, Young-Ho,Shin, Hye-Min,Chung, Young-Dong,Doh, Chil-Hoon,Jin, Bong-Soo,Kim, Hyun-Soo,Moon, Seong-In,Oh, Dae-Hui,Kim, Ki-Won 한국전기전자재료학회 2007 전기전자재료학회논문지 Vol.20 No.5

Thermal behavior of $Li_{1-x}CoO_2$ has been investigated employing DSC (Differential Scanning calorimetry) and TGA (Thermogravimetry Analyzer), and the crystal parameters were calculated from XRD (X-ray diffraction).for the commercial rectangular pouch cell(1000 mAh).The cathode materials coated over aluminium foil current collector is made up of a blend consisting of active material $LiCoO_2$(size $20\;{\mu}m$, 94 wt%), conducting material super p black (SPB, 3 wt%) and binder polyvinylidene fluoride (PVDF, 3 wt%). The anode is a mix consisting of carbon (92 wt%) and PVDF(8 wt%) coated over copper foil. The cells for the experiments were first preconditioned by cycling three times and stabilized at OCV=3.0, 3.5, 4.2, 4.35 and 4.5 V. The stabilized cathode material was used for thermal and crystal parameter investigations.

한열성향(寒熱性向)에 따른 신문혈(神門穴) 애구(艾灸) 효능(效能)의 비교(比較) 연구(硏究)

김동훈,김종덕,김은정,김경태,류성룡,정지철,박영배,Kim, Dong-hoon,Kim, Jong-deog,Kim, Eun-jung,Kim, Kyung-tae,Rhu, Seong-ryong,Jung, Ji-chul,Park, Young-bae 대한침구의학회 2004 대한침구의학회지 Vol.21 No.4

Objective: Moxibustion is one of major healing technique in oriental medicine. It has been widely used in many disease. There is an text which suggest moxibustion is more efficient to the situation of han(寒) and heo(虛) than yeal(熱) and sil(實) in Huangdineijing <黃帝內經>. The aim of this study is to research the effect of moxibustion at Shinmun(H7) is different according to cold or heat tendendy man by analyzing the electroencephalogram(EEG). Methods: We classified objects by their cold or heat tendency using questionnare for cold - heat patternization. (12 cold tendency man, 19 heat tendency man) Before and after moxibustion at Shinmun(H7), EEG raw data were measured during 5 minutes. The correlation dimension(D2), the correlation dimension variability rate(${\Delta}D2$), largest lyapunov exponent(L1) and largest lyapunov exponent variability rate(${\Delta}L1$) were calculated. We analyzed D2, ${\Delta}D2$, L1, ${\Delta}L1$ to see the effect of moxibustion at Shinmun(H7) was statistically different according to Cold or Heat tendendy man. Results : Paired t-test showed significant differences between before and after moxibustion at Shinmun(H7) on the Fp2 in D2(p<0.05), on the Fp2, F3 and F4 in ${\Delta}L1$(p<0.05). Student Hest showed significant differences between cold and heat tendendy man on the F3 in ${\Delta}L1$(p<0.05). Conclusion: These results suggest that moxibustion at Shinmun has an effect on stabilizing mind and it is more efficient to the cold tendendy man than the heat tendendy man.

다강성 나노복합체 박막

김동훈,Kim, Dong-Hun 한국전기전자재료학회 2017 전기전자재료 Vol.30 No.4

생식보조시술시 단백질원으로서 인간난포액의 적합성 및 효율성에 관한 연구;II. 인간난포액이 생쥐수정란의 체외발달에 미치는 효과

김동훈,지희준,김지연,구정진,장상식,정길생,Kim, D.H.,Chi, H.J.,Kim, J.Y.,Koo, J.J.,Chang, S.S.,Chung, K.S. 대한생식의학회 1996 Clinical and Experimental Reproductive Medicine Vol.23 No.1

This study was performed to investigate the effect of human follicular fluid (HFF) on development of mouse embryos, for evaluating the suitability of HFF as a substitutive material of human fetal cord serum in ART program. The various concentrations of HFF were added into the culture medium and the effects of HFF concentrations were examined to identify the optimal concentration of HFF for embryo development. The potency of HFF in improving embryo development was compared to that of other protein supplement. Collected HFFs were classified with the maturity of the containing oocytes; mature, immature, atretic, and then the effects of the classified HFFs on embryo development were examined. Also, HFF was separated into the low (<30,000 Da) and high (>30,000 Da) molecular weight fractions and the effects of the fractions on embryo development were investigated. The highest development rate was found in culture medium supplemented with 20% HFF, bnt this rate was reversely reduced at the concentrations of HFF higher than 20%. The development rates to the blastocyst, hatching blastocyst, attachment and outgrowth cultured in mature HFF was significantly higher than those in immature and atretic HFF, and mean cell number in blastocyst was higher in mature HFF than in immature and atretic HFF. The development rates of mouse embryos according to protein sources were significantly higher in HFF than in fetal cord serum (FCS), maternal serum (MS) and bovine serum albumin (BSA), and mean cell number in blastocyst cultured in HFF was higher than that in FCS, MS and BSA. The development rates of embryo and mean cell number in blastocyst cultured in high molecular weight fraction of HFF were higher than those in low molecular weight fraction, but the results of high molecular weight fraction were lower than those of whole HFF. Therefore, these results indicated that human mature follicular fluid was useful for improving the development of mouse embryos, which suggests a possibility that HFF also may be used efficiently for improving the culture condition in human ART program as a protein supplement.

요추간반절제술후에 발생한 복부 가성 대동맥류: 증례 보고$^1$

김동훈,Kim, Dong-Hun The Korean Radiological Society 2003 대한영상의학회지 Vol.48 No.1

요추간반 수술중 발생하는 혈관외상은 드물지만 다양한 임상증상 때문에 간과될 때는 심각한 합병증을 남긴다. 따라서 외과의사와 방사선과의사는 증상에 따라 요추간반 수술중 발생한 혈관외상을 조기진단하여 치명적인 합병증을 예방하는데 중점을 두어야한다. 최근 저자들이 경험한 요추간반 수술후 발생한 복부 가성 대동맥류를 문헌고찰과 함께 소개한다. Vascular injuries which occur during lumbar disk surgery, although rare, can give rise to potentially fatal complications which may be overlooked due to a broad range of clinical manifestations, and which surgeons and radiologists should be aware of. We report a recently encountered case of pseudoaneurysm of the abdominal aorta after lumbar disc surgery, and review the associated literature.

트위터 오피니언 마이닝을 통한 코로나19 기간 대학 비대면 수업에 대한 의견 고찰

김동훈,강정,주영준,Kim, Donghun,Jiang, Ting,Zhu, Yongjun 한국문헌정보학회 2021 한국문헌정보학회지 Vol.55 No.4

본 연구는 코로나바이러스감염증-19 (이하 코로나19) 확산 이후 대학의 부분 또는 전면 비대면 수업으로의 전환에 대해 소셜 미디어 플랫폼 중 하나인 트위터에서 이를 어떻게 생각하고 논의하고 있는지를 파악하기 위해 진행되었다. 이를 위해 트위터에서 비대면 수업 관련 트윗을 수집한 후 감성분석 및 시계열 주제 분석을 실시하였다. 감성분석결과, 전반적으로 긍정적인 여론보다 부정적인 여론이 많았지만 시간이 지남에 따라 점차 부정적인 여론이 줄어드는 경향이 나타남을 확인하였다. 또한 월별 감성점수분포를 통해 학기 중이 방학기간보다 감성점수 분포의 폭이 넓음을 확인하였고, 이를 통해 학기 중일 때가 방학 때보다 비대면 수업에 대해 더 다양한 감정과 의견을 교환한다는 사실을 확인할 수 있었다. 다음으로 긍정트윗과 부정트윗을 구분하여 시계열 주제 분석을 실시한 결과, 긍정트윗에서는 수업환경 및 장비, 긍정적인 감정 표현, 강의시청장소, 언어수업, 시험 및 과제와 같은 다섯 가지 주요한 주제가 나타났으며, 부정트윗에서는 시간(수업시간, 쉬는시간), 시험 및 과제, 부정적인 감정 표현, 수업환경 및 장비와 같은 네 가지 주요한 주제가 나타남을 확인하였다. 또한 각 주제별 대표 키워드들의 비율을 통해 시간에 따른 주제의 변화를 파악함으로써 비대면 수업에 대한 여론의 트렌드를 살펴 보고자 하였다. 본 연구는 기존 비대면 수업 관련 연구들과는 달리 소셜 미디어 중 하나인 트위터를 활용하여 국내 대학의 비대면 수업에 대한 전반적인 의견을 파악하고자 하였으며, 감성분석과 시계열 주제 분석을 활용하여 비대면 수업에 대한 긍부정 여론을 나누어 식별 및 시간의 흐름에 따른 트렌드의 변화를 파악하였다는 점에서 학문적 함의를 지닌다. 또한 연구결과는 국내 대학에서의 비대면 수업에 대한 구성 및 개선방안 등에 활용될 수 있으며, 비대면 수업을 설계하는 대학 및 교수자들에게 도움이 될 수 있다는 점에서 실질적인 함의를 지닌다. This study aimed to understand how people perceive the transition from offline to online classes at universities during the COVID-19 pandemic. To achieve the goal, we collected tweets related to online classes on Twitter and performed sentiment and time series topic analysis. We have the following findings. First, through the sentiment analysis, we found that there were more negative than positive opinions overall, but negative opinions had gradually decreased over time. Through exploring the monthly distribution of sentiment scores of tweets, we found that sentiment scores during the semesters were more widespread than the ones during the vacations. Therefore, more diverse emotions and opinions were showed during the semesters. Second, through time series topic analysis, we identified five main topics of positive tweets that include class environment and equipment, positive emotions, places of taking online classes, language class, and tests and assignments. The four main topics of negative tweets include time (class & break time), tests and assignments, negative emotions, and class environment and equipment. In addition, we examined the trends of public opinions on online classes by investigating the changes in topic composition over time through checking the proportions of representative keywords in each topic. Different from the existing studies of understanding public opinions on online classes, this study attempted to understand the overall opinions from tweet data using sentiment and time series topic analysis. The results of the study can be used to improve the quality of online classes in universities and help universities and instructors to design and offer better online classes.

M2M(Mobile to Machine) 환경에서의 유비쿼터스 기반 공작기계 운용기술

김동훈,송준엽,Kim, Dong-Hun,Song, Jun-Yeop 재료연구소 2007 機械와 材料 Vol.19 No.1

Detection of Representative Enteropathogenic Bacteria, Vibrio spp., Pathogenic Escherichia coli, Salmonella spp., Shigella spp., and Yersinia enterocolitica, Using a Virulence Factor Gene-Based Oligonucleotide Microarray

김동훈,Bok-Kwon Lee,Yong-Dae Kim,이성근,Young-Chang Kim 한국미생물학회 2010 The journal of microbiology Vol.48 No.5

Rapid identification of enteropathogenic bacteria in stool samples is critical for clinical diagnosis and antimicrobial therapy. In this study, we describe the development of an approach that couples multiplex PCR with hybridization to a DNA microarray, to allow the simultaneous detection of the 10 pathogens. The microarray was synthesized with 20-mer oligonucleotide probes that were designed to be specific for virulencefactor genes of each strain. The detection limit for genomic DNA from a single strain was approximately 10 fg. In the presence of heterogeneous non-target DNA, the detection sensitivity of the array decreased to approximately 100 fg. We did not observe any non-specific hybridization. In addition, we successfully used this oligonucleotide-based DNA microarray to identify the causative agents in clinical stool samples from patients with food-borne enteritis.