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윤기영,차미자,신현주 서원대학교 교육연구소 2000 敎育發展 Vol.19 No.2
In this paper, we attempt to outline the present conditions and problems on discussion learning in early childhood education center. Also, proper application of discussion method in order to develop an effective teaching - learning model in Early childhood education center. After the investigation of literature, in - depth interview was conducted with 20 kindergarten teachers were experienced discussion learning. We find that kindergarten teachers have a correct understanding of the importance of discussion learning in early childhood education center, but they have no basic concept for it on the spot. As a conclusion, we proposed three items of improvement for the better discussion learning in early childhood education center. 1.Provide of sufficient condition for discussion learning, such as space, time etc. 2.Offering a workshop for the enhancement of exprtise of teachers. 3.Developing various discussion learning model for the field.
윤기영,표경남,류문숙,김현식 서원대학교 교육대학원 2003 교육논총 Vol.7 No.-
The purpose of this study is to provide desirable directions for early childhood teacher education. To serve this purpose, the character on 'early childhood education theories' lectures are studied and examine the actual conditions of the 'subject education theories' lectures in university or college early childhood education Dept. And analysis the theories related subject matter. The 'early childhood education theories' lecture was characterized are as followers ; First, 'early childhood education theories' lecture means whole child education as not subject matter education. Second, 'early childhood education theories' lecture means school level education as primary(elementary) school level education. Third, 'early childhood education theories' lecture means generate subject education as like integrated curriculum, not like seperate subject education curriculum.
RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단
배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2
Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.
주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4
Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)
항진균물질을 생산하는 Streptomyces fimbriatus AF21-3의 분리 및 동정
장주현,김정완,이우윤,윤영준,이경림,김승곤,이태수 인천대학교 2000 基礎科學論文集 Vol.11 No.1
Actinomycetes were isolated from soil to find new antibiotics. An active antimicrobial compound was isolated from the culture filtrate of Actinomycetes AF 21-3. The Actinomycetes strain which showed strong antifungal activity against Candida tropicalis and Microsporum gypseum was identified as Streptomyces fimbriatus and named as Streptomyces fimbriatus AF 21-3.
윤기영,정현숙,류칠선 서원대학교 교육연구소 2001 敎育發展 Vol.20 No.2
The purpose of this study was to develop a in-service teacher education program for student teaching in early childhood education center in order to reinforce student teacher's educational role for improving their student teaching. In this paper, the sample consisted of 20 early childhood professionals who have experienced at least 3 years in students teaching program. The survey was administered using the Delphi method. The study explored the objectives, contents, administration and method, and evaluation of the developed program. We also attempt to outline the present conditions and problems on student teaching in early childhood education center. As a conclusion, the program have to assist the able kindergarten teacher for understanding student teacher correctly, to consist and administrate a practicum program with systematic support.