서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Mechanical Analysis of Woven Composites at High Strain Rates and Its Application to Predicting Impact Behavior

( Han Sun Ryou ),( Kwan Soo Chung ),( Ji Ho Lim ) 대한금속재료학회 ( 구 대한금속학회 ) 2008 METALS AND MATERIALS International Vol.14 No.6

The deformation behavior of woven composites at high strain rates was analyzed using a constitutive equation developed to describe the nonlinear, anisotropic/asymmetric and rate-dependent mechanical behavior of woven composites. The rate-dependent nonlinear behavior of woven composites was characterized at high strain rates (1 s-1 to 100 s-1) using a tensile testing method first proposed in this research. The material properties for the developed constitutive equation were determined and subsequently used in a finite element analysis of the deformation behavior of woven composites at high strain rates. Finally, the impact behavior of woven composites was predicted using the constitutive equation and the results were compared with experiments, showing that the current constitutive equation including the characterization method is adequate to describe the deformation behavior of woven composites at high strain rates up to impact level.

Numerical Evaluation of Crashworthiness of Automotive Sheets

Ryou, Han Sun,Lee, Myoung Gyu,Kim, Chong Min,Chung, Kwan Soo Trans Tech Publications, Ltd. 2007 Key Engineering Materials Vol.345-346 No.-

<P>Crash simulations were performed for automotive sheets. To understand the influence of crystal structures in sheet materials on crashworthiness, the effect of the yield function shape was studied by adopting the recently developed non-quadratic anisotropic yield surface, Yld2004-18p. The effect of the back-stress was also investigated by comparing simulation results obtained for the isotropic, kinematic and combined isotropic-kinematic hardening laws based on the modified Chaboche model. In addition, the effects of anisotropy and sheet thickness on crashworthiness were evaluated.</P>

공동체 제의의 전승과 지역민의 실천 -옛 자인현 한장군 제의를 중심으로-

류한선(Ryou, Han Sun),이은정(Lee Eun Jeong) 비교민속학회 2021 비교민속학 Vol.- No.74

Session 2. 곤충매개 인수공통전염병: Vectorborne Zoonotic Diseases : S2-2 ; Severe Fever with Thrombocytopenia Syndrome: Molecular and Serological Diagnosis

( Sun Whan Park ),( Jung Sang Ryou ),( Seok Min Yun ),( Chan Park ),( Won Ja Lee ),한명국 ( Myung Guk Han ) 대한인수공통전염병학회 2014 창립총회 및 학술대회 초록집 Vol.2014 No.1

Severe fever with thrombocytopenia syndrome (SFTS) is a new emerging viral infectious disease, first reported in China in 2010. Patients with SFTS have been reported recently in South Korea and Japan in 2013. Totally 36 cases of SFTS were identified in Korea in 2013 with case fatality rate of 47%. The SFTS is characterized by acute febrile illness, thrombocytopenia, leucopenia, gastrointestinal symptoms, elevated serum enzymes and multi-organ failure which are not specific signs and symptoms for SFTS. The SFTS virus (SFTSV) causing SFTS belongs to the genus Phlebovirus in the family Bunyaviridae. Heartland viruses and Hunter Island Group virus (HIGV) which are related to but distinctly different from SFTSV were isolated from patients in the US and ticks in Australia, respectively. The patients infected with Heartland virus presented a similar signs and symptoms to SFTS. HIGV was isolated from ticks collected from shy albatross on Albatross Island, a small island in the Hunter Island Group in northwestern Tasmania, Australia. The SFTSV has been detected in Haemaphysalis longicornis and Rhipicephalus microplus ticks suggesting that the causative agent of SFTS, SFTSV is transmitted possibly to humans by ticks, such as H. longicornis which is considered as the principal vector of SFTSV. Recently Amblyomma testudinarium and Ixodes nipponensis are also implicated as the vector of SFTSV. Although cases of person-to-person transmission through contact with infected patient’s blood or mucous have also been reported in China, transmission of SFTSV takes place by biting of ticks infected with SFTSV. The SFTS presents with clinical manifestations similar to those of other infectious vector-borne diseases, such as hemorrhagic fever with renal syndrome (HFRS), scrub typhus, leptospirosis and anaplasmosis, strongly suggesting the need for differential diagnosis of SFTS from other infectious diseases. HFRS, leptospirosis and anaplasmosis are caused by Hantavirus, Leptospira interrogans and Anaplasma phagocytophilum, respectively. Wild rodents (Apodemus agrarius) play the role of the primary natural reservoir for these pathogens. HFRS, scrub typhus and leptospirosis are endemic in eastern and south-east Asia including Korea. In terms of distribution of hosts and reservoirs of vector-borne pathogens in the environment, and current coexistence of these diseases in the same epidemic area, concurrent infections of these vector-borne diseases can occur. Therefore, a reliable SFTSV detection tool is urgently required to provide early diagnosis of SFTS to support clinical care, infection control and epidemiological surveillance. Laboratory diagnosis of SFTSV infection is carried out by various ways, including nucleic acid amplification, detection of viral antigen, virus isolation and antibody detection to SFTSV using by real-time RT-PCR, Vero E6 cell culture, enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent assay (IFA). Conventional RT-PCR and real-time RT-PCR was developed in our laboratory and applied to detect SFTSV from hospitalized patients who presented SFTS symptoms. The SFTSVs were detected from 35 specimens among more than 300 serum specimens in 2013 and the nucleotide sequence was analyzed for identification of the SFTSV. The Korean isolates of SFTSV showed 93-98% similarityof the nucleotide sequences to Chinese and Japanese isolates of SFTSV and were distinctly different from Heartland virus. SFTSVs were also isolated by Vero E6 cell culture and identified by the nucleotide sequence, IFA using monoclonal antibodies, and electron microscopy. As reported, antibody to SFTSV can be detected as early as 2 to 4 days after illness onsets by serological assays and persists in some patients even one year after recovery. Seroconversion against SFTS V in patients with SFTS occurs mostly more than 3 weeks after onsets of illness. We developed IFA for laboratory diagnosis and determined serologic cross reactivity of SFTSV to Hantavirus and rickets. Slides for IFA were prepared with Vero E6 cells infected with SFTSV and Hantaan virus (HTNV). Commercial IFA kits for Anaplasma, Ehrlichia, Leptosira and Origentia spp were also used in the study. Serum specimens of SFTSV patients, sera of HTNV IgG antibody of more than 512 and paired sera of scrub typhus patients were tested with IFA. None of sera specimens showed specific antibody reaction to SFTSV infected cells and antigens assayed by IFA. IgG titers to each homogeneous antigen of HTNV and O.tsutsugamushi assayed by IFA were ranged from 512 to 4,096 and from 0 to 8,192. These results suggest that SFTSV does not have cross reactivity to at least, HTNV and O. tsutsugamushi. Considered currently occurrence of SFTSV, HTNV and O. tsutsugamushi in the same epidemic of the country, concurrent infection can be identified by serological assays. In the presentation, we report a case of coinfection with SFTSV and Hantavirus in humans. To the best of our knowledge, this is the first case report of coinfection with SFTSV and Hantavirus. A 66-year-old farm-dwelling woman was admitted to the hospital with a 6-day history of worsening fever and myalgia. Neutropenia and thrombocytopenia were evident on admission to the intensive care unit. SFTSV infection was suspected based on clinical findings and laboratory test results, although the patient had no recollection of a tick bite and there was no evidence of tick bites. She was treated with plasma exchange and oral ribavirin (4.0g/day) after 10 days of illness onset and had fully recovered at 15 days after illness onset Considering the concurrence of SFTS, HFRS and ricketiisial diseases in the endemic area and the higher possibility of exposure to pathogens due to the patient’s area of residence and occupation as a farmer, coinfection with SFTSV, hantavirus and ricketiisial agents in the patient is suggested. Determining the effects of coinfection on disease prognosis and laboratory diagnosis could be helpful in deciding patient treatment and management.

Serum MicroRNA Expression Profiling in Mice Infected with Rabies Virus

Han, Myung Guk,Park, Jun-Sun,Lee, Cho Soon,Jeong, Young Eui,Ryou, Jung Sang,Cho, Jung Eun,Ju, Young Ran,Lee, Kyoung-Ki Korea Centers for Disease Control and Prevention 2011 Osong Public Health and Research Persptectives Vol.2 No.3

<P><B>Objectives</B></P><P>Serum or plasma microRNAs (miRNAs) are potential biomarkers for the diagnosis for cancer and prenatal diseases. This study was conducted to investigate whether rabies virus causes a change in serum miRNA expression.</P><P><B>Methods</B></P><P>ICR mice were intramuscularly inoculated with rabies virus and were sacrificed weekly to collect serum and brain tissue for 4 weeks postinoculation. Mice were assigned to four groups based on the results of indirect immunofluorescent assays, enzyme-linked immunosorbent assay, and nested reverse transcription-polymerase chain reaction and the expression profiles of serum miRNAs were compared using a commercial mouse miRNA expression profiling assay.</P><P><B>Results</B></P><P>The expression levels of miRNAs changed significantly with the different stages of the disease. The expression level of 94 serum miRNAs in infected mice changed at least twofold. Seven microRNAs of them were significantly upregulated or downregulated in all infected mice regardless of disease status. The number of miRNAs with an expression level change decreased with the progression of the disease. In a hierarchical cluster analysis, infected mice clustered into a group separate from uninfected control mice.</P><P><B>Conclusions</B></P><P>Based on the relationship of miRNAs to gene expression regulation, miRNAs may be candidates for the study of viral pathogenesis and could have potential as biomarkers.</P>

Hantaan virus nucleocapsid protein stimulates MDM2-dependent p53 degradation

Park, Sun-Whan,Han, Myung-Guk,Park, Chan,Ju, Young Ran,Ahn, Byung-Yoon,Ryou, Jungsang Society for General Microbiology 2013 The Journal of general virology Vol.94 No.11

<P>Apoptosis has been shown to be induced and downregulated by the Hantaan virus (HTNV) nucleocapsid (N) protein. To address these conflicting data, expression of the p53 protein, one of the key molecules involved in apoptosis, was assessed in the presence of the N protein in A549 and HeLa cells. The amount of p53, increased by drug treatment, was reduced when cells were infected with HTNV or transfected with an expression vector of the HTNV N protein. When cells were treated with a proteasome inhibitor (MG132) or an MDM2 antagonist (Nutlin-3), <I>p53</I> expression was not reduced in N protein-overexpressed cells. We concluded that the HTNV N protein ubiquitinates and degrades p53 MDM2-dependently. Here we report downregulation of p53 expression through a post-translational mechanism: MDM2-dependent ubiquitination and degradation by the HTNV N protein. These results indicate that N protein-dependent p53 degradation through the ubiquitin proteasome system is one of the anti-apoptotic mechanisms employed by HTNV.</P>

손바닥 선인장 열매 섭취가 한림지역 해녀의 혈중지질, 혈소판 응집성 및 항산화 효과에 미치는 영향

한순금(Han, Sun-Geum),강민숙(Kang, Min-Sook),류성희(Ryou, Sung-Hee),황승욱(Hwang, Sung-Wok),강정숙(Kang, Jung-Sook) 한국영양학회 2012 Journal of Nutrition and Health Vol.45 No.5

We investigated dietary effects of prickly pear cactus (Opuntina ficus-indica) on plasma lipids, platelet aggregation (PA), hemolysis, plasma TBARS and liver enzymes. Twenty eight volunteer diving women in Jeju island had daily 20 g cactus tea containing 27% prickly pear cactus (PPC) powder for 4 weeks, and data for the study subjects were analyzed, on the basis of diagnostic criteria for blood pressure (BP)(≥ 140/90 mmHg), plasma cholesterol (≥ 200 mg/dL) and triglyceride (≥ 150 mg/dL). The subjects with higher BP had higher plasma total cholesterol (TC) and triglyceride (TG) concentrations than those with normal BP. Those with higher TC also had higher TG. Subjects with normal BP or normal TC had higher initial slope of PA than their higher counterpart in BP and TC. PPC intake decreased plasma TG in those with higher BP. PPC intake significantly decreased the elevated initial slope in groups with normal BP, TC, and TG. Hemolysis after PPC intake decreased significantly in all the subjects and plasma TBARS decreased in the subjects with higher plasma TC and higher TG. Glutamic-oxaloacetic transaminase (GOT) significantly increased and total bilirubin significantly decreased in all the subjects after PPC intake. The present study with diving women showed that beneficial effects of short term intake of prickly pear cactus might differ depending on the subject conditions in term of blood pressure, and plasma lipids. However, long term usage of prickly pear cactus may provide preventive effects of cardiovascular diseases to all the population, presumably by hypolipidemic, antithrombotic, and antioxidant actions of its bioactive flavonoids and soluble fiber. (Korean J Nutr 2012; 45(5): 462 ~ 469)

Epidemiological and Clinical Features of Severe Fever with Thrombocytopenia Syndrome during an Outbreak in South Korea, 2013–2015

Park, Sun-Whan,Ryou, Jungsang,Choi, Woo-Young,Han, Myung-Guk,Lee, Won-Ja Allen Press, etc 2016 The American journal of tropical medicine and hygi Vol.95 No.6

<P>Since the first reported case of severe fever with thrombocytopenia syndrome (SFTS) in South Korea in 2013, between 2013 and 2015, we collected 1,697 serum samples from suspected patients who experienced symptoms of SFTS. We performed reverse transcriptase polymerase chain reaction using total RNA extracted from the patients' sera. When viral RNA was detected in the sera, SFTS was diagnosed. Among the 1,697 samples, 170 were positive for SFTS virus. We then analyzed the epidemiologic features of these 170 cases. As a result, we found that the annual number of cases increased steadily. However, the annual case fatality rate exhibited a downward trend. The majority of patients were aged ≥ 60 years, and most cases occurred during May–October in the eastern and southern parts of the country. These results may be useful for effective SFTS control by describing the clinical and epidemiologic features of the disease in South Korea.</P>

Severe Fever with Thrombocytopenia Syndrome Virus, South Korea, 2013

Park, Sun-Whan,Han, Myung-Guk,Yun, Seok-Min,Park, Chan,Lee, Won-Ja,Ryou, Jungsang U.S. Department of Health and Human Services * Cen 2014 Emerging Infectious Diseases Vol.20 No.11

<P>During 2013, severe fever with thrombocytopenia syndrome was diagnosed in 35 persons in South Korea. Environmental temperature probably affected the monthly and regional distribution of case-patients within the country. Phylogenetic analysis indicated that the isolates from Korea were closely related to isolates from China and Japan.</P>