백서 간재생중 H2B Histone 유전자 발현에 대한 Octamer Binding Transcription Factor의 역할

임규,방재웅,최두식,손미영,이영철,박청,김태동,김영래,박종일,윤완희,박승길,황병두 한국노화학회 2001 한국노화학회지 Vol.11 No.1

감압하에서의 김치숙성과 열처리

정자림,김미향,김미정,장경숙,김순동 대구효성가톨릭대학교 식품과학연구소 1994 식품과학지 Vol.6 No.-

김치의 숙성과 열처리 효과에 미치는 감압의 영향에 대한 실험결과를 요약하면 다음과 같다. 김치를 560㎜Hg과 360㎜Hg에서 숙성시킨 경우는 상압보다 젖산균의 생육이 촉진되는 반면호기성균이 감소되어 김치의 품질이 향상되었다. 그러나 0㎜Hg에서는 균의 생육이 저해됨과 동시에 김치조직이 손상되었다. 560㎜Hg와 360㎜Hg에서 숙성시킨 김치는 상압에서 숙성시킨 김치보다 저장성이 높았다. 숙성된 김치를 감압하에서 열처리할 경우 바람직한 감압조건은 460-260㎜Hg이었으며 80℃에서는 4분처리, 100℃에서는 2분처리, 120℃에서는 1분간 처리하는 것이 조직의 상태나 저장면에서 양호하였다. This study was carried out to investigate the effect of sub-atmosphere on the fermentation and heat treatment after fermentation of Kimchi. When the results from the conditions of atmosphere and under-atmosphere were compared, the growth of lactic acid bacteria was increased but the growth of aerobic bacteria was decreased under 560㎜Hg and 360㎜Hg. The number of total microorganism was decreased and simultaneously damaged to the tissue of Kimchi under 0㎜Hg. Kimchi fermented under 560㎜Hg and 360㎜Hg had longer storage duration than that of atmosphere. Among the several conditions, the result of heat treatment under 460-260㎜Hg was the best. The treatment for 4 minutes at 80℃, 2 minutes at 100℃, and 1 minute at 120℃ was good in tissue states and storage.

감과실의 연화에 따른 물성 변화와 헤미셀루로즈의 변화

김순동,임영숙,조성경,김미향 대구효성가톨릭대학교 식품과학연구소 1992 식품과학지 Vol.4 No.-

감의 연화에 따른 물성 변화와 이에 따른 세포벽 다당류의 조성과 함량 및 column chromatography를 통한 계략적인 분자량의 변화를 조사하였다. 조세포벽의 함량은 연화된 감에서 현저히 감소되었다. 또한 온도변화에 따른 감의 물성변화와 세포벽 다당류의 변화를 조사하였다. 그 결과 25℃에서 가장 쉽게 연화 되었으며 45℃에서는 연화가 억제되었다. 연화가 진행되는 동안에 부착성은 증가하였으나 경도는 감소되었다. 이러한 현상은 25℃에서 가장 현저하게 나타났다. 조세포벽의 함량은 연화된 감에서 현저히 감소되었은데 구체적으로 ionically associated pectin(IAP)은 59%, covalently bounded pectin(CBP)은 60%, hemicellulose fraction 2(HF2)는 74%가 감소된 반면 hemicellulose fraction 1(HF1)과 cellulose fraction(CF)은 큰 변화가 없었다. 연화된 감의 IAP, CBP에서는 pentose와 hexose의 감소율이 높았다. HF1과 HF2역시 uronic acid의 함유율이 높았는데 HF2가 현저하였다. 또 연화에 따라 HF1은 pentose와 HF2는 uronic acid와 hexose의 손실율이 높았다. 특히 HF2는 수백만 단위의 거대분자가 만단위까지 저분자화 하였다. 이상의 결과에서의 감의 연화는 CBP에 연결된 hemicellulose중 특히 HF2의 저분자화와 밀접한 관련이 있는 것으로 판단 되었다. The changes of cell wall polysaccharides were studies by gel filteration chromatography. Various cell wall polysaccharides such as ionically associated pectin(IAP), covalently bounded pectin(CBP), 2N potasium hydroxide soluble hemicellulosic fraction(HF1) and 0-3N potasium hydroxide soluble hemicellulosic fraction(HF2) were fractionated by chemical method. The content of crude cell wall remarkably decreased in the soft persimmon. The changes of texture, composition of cell wall polysaccharides during preserving of persimmon at various temperature were estimated to investigate the softening characteristics of persimmon fruit. The softening of persimmon was the most promoted at 25℃, Whereas it was inhibited at 45℃. During softening adhesiveness increased and hardness decreased. This phenomenon was obvious at 25℃. The decreasing rates of IAP, CBP and HF2 were 59, 60 and 74%, respectively, while HF1 and cellulose changed only a little during softening. The loss rate of pentose in IAP, of hexose in CBP, of hexose and uronic acid in HF2, of pentose in HF1 increased during softening. Though apparent molecular weight of all polysaccharides shifted from high molecular weight to low molecular weight polymer, the shifting degree of CBP and HF2 was especially remarkable during softening. It is suggested that the servere softening phenomenon of persimmon involved the degradation and dissolution of wall bounded-CBP and HF2 which were associated with each other.

부천세종병원에서 시행된 부검의 분석

오미혜,서정욱,명나혜,서연림,송계용,윤동헌 世宗醫學硏究所 2000 世宗醫學 Vol.17 No.1

수산화칼슘 처리된 Porphyromonas endodontalis Lipopolysaccharide가 다형핵백혈구의 IL-1과 TNF-α 생성에 미치는 영향에 관한 연구

박찬제,박동성,유현미,오태석,임성삼 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.5

Bacterial lipopolysaccharide (LPS) plays a major role in stimulating the synthesis and release of the principal osteoclast-activating cytokines , namely, interleukin 1 and tumor necrosis factor-α from immune cells, Although monocytes/macrophages are the main producers of these cytokines, recent evidence has indicated that polymorphonuclear leukocytes (PMN) have the ability to release IL-1 and TNF-α. Calcium hydroxide has been shown to be an effective medicament in root canal infectionsm, reducing the microbial titre within the canal. It has been proposed that the therapeutic effect of Ca(OH)_2 may also be the result of direct inactivation of LPS. The purpose of this study was to investigate whether treatment of Porphyromanas endodonatalis LPS with calcium hydroxide alters its biological action as neasured by human PMN secretion of IL-1 and TNF-α, and it was compared with Escherichia coli LPS. P.endodonatalis ATCC 35406 was cultured in anaerobic condition, and LPS was extracted using the hot-phenol water extraction method and purified. Purchased E. coli LPS was also purified . 100 ㎍/ml of each LPS in pyrogen free water were incubated with 25mg/ml Ca(OH)_2 at 37℃ for 7 days. The supernatants were subjected to ultrafiltration. And the isolates were lyophilized and weighed. PMNs were obtained from peripheral blood by centrifugation layered over Lymphoprep. The cells were resuspended (4 × 10^6 cells/ml) in RPMI 1640 followed by treatment with various concentrations of LPS (0, 0.1, 1, 10㎍/ml) for 24 hours at 37℃ in 5% CO_2 incubator. The supernatants of cells were collected and the levels of IL-1α, IL-1β and TNF-α were measured by enzyme-linked immunosorbent assay. The results were as follows; 1. The levels of IL-1α, IL-1β, TNF-α from PMN treated with each LPS were significantly higher than those released from unstimulated PMN of the control group (p<0.05). 2. The levels of all three cytokines released from PMN stimulated with each calcium hydroxide treated LPS were significantly lower than those released from PMN stimulated with each untreated LPS (p<0.05). while they were not significantly different from those released from unstimulated PMN of the control group (p>0.05). 3. The levels of secretion or all three cytokines were affected in a dose-dependent manner in PMN stimulated with each LPS (p<0.05), but not in PMN stimulated with each calcium hydroxide treated LPS (p>0.05). 4. The levels of all three cytokines released from PMN stimulated with P.endodontalis LPS were significantly lower than those released from PMN stimulated with E. coli LPS (p<0.05).

급성 복통을 주소로 진단된 자발성 양측 부신 출혈에 의한 부신피질부전

조아름,홍주영,김동호,박민지,권미혜,박근용,원희관,임동미 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.S

Thomas Addison은 1855년 발표된 그의 논문에 일차성 부신피질부전에 의하여 발생한 근력 약화, 피로, 식욕부진, 복통, 체중감소, 색소침착 등에 대하여 처음으로 기술하였다[1,2]. 부신피질부전은 부신피질의 파괴에 의해 발생하는 당질 코르티코이드 결핍과 관련된 일차성과 뇌하수체에서 혈청 부신피질자극 호르몬(adrenocorticotropic hormone, ACTH)의 분비장애에 의해 발생하는 이차성으로 구분할 수 있다. 일차성과 이차성의 중요한 차이는 무기질 코르티코이드의 결핍이 일차성에는 있고 이차성에 는 없다는 점이다. 일차성 부신피질부전의 원인으로 이전에는 결핵에 의한 양측 부신 파괴가 가장 흔한 원인이었으나, 결핵 진단율 및 치료약제 보급률이 좋아진 20세기 후반부터는 감소 추세를 보이고 있으며, 선진국에서는 자가면역성 부신염에 의한 일차성 부신피질 부전이 80~90%로 가장 흔한 것으로 보고되었다[3]. 이외에 다른 감염성 질환, 전이성 암, 림프종, 부신출혈 또는 경색, 약물 등의 원인을 들 수 있다. 일차성 부신피질부전의 임상 증상과 징후로는 전신무력감, 식욕부진, 피로감, 체중감소, 과다한 색소침착, 오심, 복통, 기립성 저혈압, 저혈당, 전해질 장애 등이 올 수 있고, 심하면 탈수, 저혈압, 의식장애 및 쇼크가 동반될 수 있다. 일차성 부신피질부전은 본 증례와 같이 드물게 양측 부신 출혈성 경색으로 인해 발생되는 경우가 보고되었는데, 위험인자로는 항응고제 또는 헤파린 투여, 혈전성 질환, 항인지질 증후군과 같은 과응고 상태, 물리적 외상, 감염, 심한 스트레스와 관련되어 발생할 수 있으며 드물게 임신, 부신 종양, 쇼크 등이 원인이 되고 있다[4,5]. 그러나 본 증례와 같이 원인 없이 발생한 자발성 출혈에 의한 부신피질부전의 보고는 매우 드물다. 양측 부신 출혈의 임상 증상과 징후로는 저혈압, 쇼크, 복부, 등, 옆구리, 흉부의 통증, 발열, 의식저하, 복부 강직, 반발 압통 등이 있다[6]. 적절한 치료를 시행하지 않으면 쇼크가 진행하여 혼수와 사망에 이를 수 있어 정확하고 빠른 진단과 치료가 필요하다. 환자가 생존한다면 부신 기능은 드물게 수개월 후 정상으로 회복될 수 있다[7]. 저자들은 상복부 통증을 주소로 내원한 환자에서 매우 드물게 원인 없이 발생한 자발성 양측 부신 출혈에 의한 급성 부신피질부전 1예를 경험하였기에 보고하는 바이다.

産後 諸般 症狀에 對한 臨床的 考察

嚴殷奭,李東寧,林垠美 대한한의학회 부인과학회 2001 大韓韓方婦人科學會誌 Vol.14 No.1

The purpose of this study is to investigate the various postpartum symptoms, and provide fundamental data about postpartum health care. The subjects of the present study were 148 women (74 women who completed vaginal delivery and 74 women who delivered by cesarean section) who completed labor between January 1 and June 30, 1999, at the department of OB & GYN in Dong-Inchon Gil Hospital. We investigated the various symptoms which occurred from the moment of hospital discharge to 5 months after parturition, and examined the frequency of symptoms. Then we systemically classified the symptoms. Next, we subdivided the major categories of symptoms. After that, we classified the symptoms by the age of patients, the obstetric history of patients, and the method of delivery. Finally we compared the symptoms of these results and the standard textbook (Textbook of Oriental Gynecology edited by Byoung-Key Song, 1994). The results of this investigation were summarized as follows: 1. Systemic classification of various postpartum symptoms: (1) Nervous and psychiatric symptoms accounted for 14.2% of the postpartum symptoms; (2) circulatory symptoms, for 23.7%; (3) respiratory symptoms, for 7%; (4) gastrointestinal symptoms, for 10%; (5) musculoskeletal symptoms, for 7.3% of which waist and hip joint accounted for 33% of the arthralgia; knee joint, for 25%; wrist joint, for 13.7%; (6) urinary symptoms, for 2.7%; (7) reproductive symptoms, for 23.3%; (8) the rest, for 11.8%. 2. Subdivision the major categories of various postpartum symptoms: (1) Discomfort at the incision site accounted for 92.6% of the 148 women; (2) increase of sweating, for 77%; (3) arthralgia, for 66.9%; (4) common cold, for 63.5%; (5) hair loss, for 62.2%; (6) dizziness, for 52.7%; (7) depression, for 47.3%; (8) toothache, for 43.2%; (9) coldness of the extremities and lower abdomen, for 37.8%; (10) edema, for 37.2%. 3. There were no remarkable differences in the frequency of symptoms according to the age of patients, the obstetric history of patients, and the method of delivery. 4. We observed some symptoms not mentioned in the textbook. On the other hand, we did not observe some of the textbook symptoms. Thus, if we continue investigation about various postpartum symptoms by other methods (incidence duration, symptoms' degree, etc) and compare with the textbook, we can update the textbook. There will be significant data which are helpful for diagnosis and oriental medical treatment of the patient after parturition.

산채류의보존과 일본식 절임

오영애,하귀현,박인경,김미향,김미정,김미경,정자림,이명숙,김순동 대구효성가톨릭대학교 식품과학연구소 1992 식품과학지 Vol.4 No.-

This paper showed the summarization of the preservative method of wild vegetables in the house and the precessing method of Japanese pickles. The preservative methods of wild vegetables were explained devided into long, short term, fresh state, heat treatment and drying. Salt, soybean fermented sauce, soybean fermented paste, lees of refined rice wine and residue in the preparation of bean curd were used as the materials of Japanese pickles.

장기간 보관한 멸균물품의 오염 상태에 관한 연구

주홍순,조순덕,정재심,신동분,임바올라,노은지,이선희,송영미,최정화,백병윤,김미나 대한병원감염관리학회 2003 의료관련감염관리 Vol.8 No.1

Hepatocyte nuclear factor 1-alpha mutation in normal glucose-tolerant subjects and early-onset type 2 diabetic patients

( Dong Mee Lim ),( Nam Huh ),( Keun Yong Park ) 대한내과학회 2008 The Korean Journal of Internal Medicine Vol.23 No.4

Background/Aims: The prevalence of diabetes in Korea is reported to be approximately 10%, but cases of maturity-onset diabetes of the young (MODY) are rare in Korea. A diagnostic technique for autosomal dominant MODY is being actively sought. In this regard, we used a DNA chip to investigate the frequency of mutations of the MODY3 gene (hepatocyte nuclear factor-1α) in Korean patients with early-onset type 2 diabetes. Methods: The genomic DNA of 30 normal individuals [age, 24.9±8.6 years] and 25 patients with early-onset type 2 diabetes (age, 27±5.9 years) was extracted, and the MODY3 gene was amplified. The amplified DNA was hybridized onto a MODY3 chip, which has oligonucleotides of 15-25 bases, representing wild-type and mutant MODY3 sequences in both forward and reverse orientations, immobilized on its surface. Results: Among the normal subjects, there was no mutation of MODY3. Among those with early-onset type 2 diabetes, there was one case of MODY3 mutation. Conclusions: Our results indicate that MODY3 mutations are not rare in Korean early-onset type 2 diabetes patients in Korea and suggest that MODY3 mutations in patients with early-onset type 2 diabetes need to be further evaluated.